The invention relates to a detection method of selenium forms in plants. The method comprises the steps of: 1, previous preparation of detection: determining instruments and reagents for detection; 2, establishing high performance liquid chromatography and inductively coupled plasma mass spectrometry, that is, HPLC-ICP-MS hyphenated techniques; 3, establishing chromatograms of five selenium form standard solutions; 4, pretreatment of plant samples and extraction and detection of selenium forms: cleaning plant materials to be detected with ultrapure water, carrying out freeze drying, smashing and filtering, measuring the water content, sealing, placing and storing at 4 DEG C for standby; or performing ultrasonic extraction at 37 DEG C for 30 minutes by enzyme immediately, taking supernatant after centrifugalization, detecting the selenium forms according to the HPLC-ICP-MS hyphenated techniques, and calculating the content of selenium forms in plants according to peak areas corresponding to the selenium forms in the samples shown in collected maps. According to the method for detecting the selenium forms in plants, forms are separated completely, pretreatment of samples is simple, convenient and rapid, the moving phase is single in component and simple to prepare, the repeatability and the precision are good, and the quantitative result is accurate and reliable.