Detection method of selenium forms in plants

Abstract

The invention relates to a detection method of selenium forms in plants. The method comprises the steps of: 1, previous preparation of detection: determining instruments and reagents for detection; 2, establishing high performance liquid chromatography and inductively coupled plasma mass spectrometry, that is, HPLC-ICP-MS hyphenated techniques; 3, establishing chromatograms of five selenium form standard solutions; 4, pretreatment of plant samples and extraction and detection of selenium forms: cleaning plant materials to be detected with ultrapure water, carrying out freeze drying, smashing and filtering, measuring the water content, sealing, placing and storing at 4 DEG C for standby; or performing ultrasonic extraction at 37 DEG C for 30 minutes by enzyme immediately, taking supernatant after centrifugalization, detecting the selenium forms according to the HPLC-ICP-MS hyphenated techniques, and calculating the content of selenium forms in plants according to peak areas corresponding to the selenium forms in the samples shown in collected maps. According to the method for detecting the selenium forms in plants, forms are separated completely, pretreatment of samples is simple, convenient and rapid, the moving phase is single in component and simple to prepare, the repeatability and the precision are good, and the quantitative result is accurate and reliable.

Description

technical field [0001] The invention relates to a method for detecting the form of plant selenium. Background technique [0002] Selenium is an essential trace element for the human body. It has anti-cancer and anti-cancer effects, scavenging free radicals in the body and anti-aging effects. Plants are biochemical factories for the synthesis of natural organic selenium. Plant organic selenium has high bioavailability and biological activity. The measurement of the biological function level of selenium is not only related to the total selenium content, but also related to the chemical form of selenium. Therefore, the detection of selenium Different chemical forms have their important practical significance. As a selenium-enriched carrier, plants can convert inorganic selenium into organic selenium, but the enzymes required in the metabolic process of selenium are extremely complex, and the instability of selenium affects people's further understanding of the form of selenium...

AI Technical Summary

Problems solved by technology

As a selenium-enriched carrier, plants can convert inorganic selenium into organic selenium, but the enzymes required in the metabolic process of selenium are extremely complex, and the instability of selenium affects people's further understanding of the form of selenium. The current detection method of selenium The research is developing from trace detection to selenium speciation detection, so practical detection and analysis methods, simplification and standardization of instruments are an important direction of selenium speciation research

In recent years, electrospray mass spectrometry (ES-MS) has been widely used in the analysis of selenium-rich plants (garlic, onion, nuts), but its sensitivity and accuracy are not high; with the development of inductively coupled plasma mass spectrometry (ICP- With the development of collision reaction cell technology in MS), high performance liquid phase and inductively coupled plasma mass spectrometry HPLC-ICP-MS technology will become a kind of plant selenium speciation analysis with its high separation efficiency and low detection limit. New technical means, no relevant data reports have been found after searching

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