58 results about "Polysaccharide peptide" patented technology

The invention belongs to the field of monosaccharide component analysis, and in particular relates to a method for detecting monosaccharide components in rainbow conk glycopeptide. The method comprises the following steps of: carrying out a test on HPLC (High Performance Liquid Chromatography) chromatographic condition and systematic compatibility; subsequently respectively absorbing a mixed reference solution and a test solution to be injected into the HPLC chromatographic instrument so as to obtain a chromatogram, wherein the reference solution is a 1phenyl-3-methyl-5-pyrazolone derivative solution of D-glucose, D-mannose, D-xylose, L-xylose, D-galactose, D-arabinose, D-hydrochloric acid glucosamine and L-rhamnose reference solutions; and the test solution is a 1-phenyl-3-methyl-5-pyrazolone derivative solution of a rainbow conk glycopeptide acid hydrolysis product. Polysaccharide peptide products such as rainbow conk glycopeptide, rainbow conk intracellular glycopeptide, rainbow conk extracellular glycopeptides and coriolus versicolor polysaccharide are distinguished by detecting the mole ratio of eight monosaccharide ingredients in a test sample, so that the quality and the curative effect of the rainbow conk glycopeptide are ensured.

The present invention belongs to the field of Chinese medicine technology, in the concrete, it relates to a Chinese medicine composition containing several ganoderma effective components and its preparation method. Said invention uses Chinese medicinal material ganoderma as main raw material, and adopts the processes of dynamic extraction and spray-drying, then detects the contents of several ganoderma effective components and yield of ganoderma extract, and adds the wall-broken ganoderma spore, and uses the contents of 3-50% of ganoderma polysaccharide, 0.2-10% of triterpene and 0.1-15% of polysaccharide peptide as quality standard. The invented Chinese machine composition can be made into powder preparation, capsule preparation, tablet preparation and soft capsule preparation.

The invention discloses an active Ganoderma lucidum polysaccharide peptide reference substance, and a preparation method and application of the reference substance, and provides an active Ganoderma lucidum polysaccharide peptide reference substance for measuring and evaluating the quality of Ganoderma lucidum and Ganoderma lucidum products. The reference substance can correct the errors in polysaccharide measurement generated using glucose as the reference substance after addition of starch as an adjuvant during the deep processing process of Ganoderma lucidum. The preparation method uses Ganoderma lucidum as a raw material and comprises the steps of soaking in water, boiling, collecting an extractive solution, concentrating, centrifuging, dialyzing, entrapping polysaccharide protein GL-PP with an active macromolecular site, further carrying out ethanol gradient separation of the GL-PP, centrifuging, and collecting precipitates; and separately carrying out column separation, and selecting active polysaccharide peptide GL-PPS as the reference substance for detecting polysaccharide peptides, wherein the reference substance can be used for accurate measurement of the content of trace active polysaccharide peptides in a UV (ultraviolet) instrument. The pure product of the reference substance disclosed by the invention can be used for detecting the quality of Ganoderma lucidum products, and can eliminate the interference of starch and other adjuvants in the Ganoderma lucidum products, so as to improve the quality standards of Ganoderma lucidum products.

The invention discloses a synchronous production technology for preparing a dendrobium officinale oral liquid and a piece beverage, which is a method for extracting polysaccharide by exploding dendrobium plant cells combined an electronic program control (UHP) technology and ultrasonic water jet, and completely releases and dissolves peptidoglycan which is difficult to be extracted from dendrobium, and synchronously prepares an oral liquid or a beverage decoction piece. Low temperature, superhigh pressure and continuous flow cell disruption enables the plant cells to be instantly exploded and released, and under action of energy shearing effect, pressure intensity cavity effect and instant explosion effect, the plant cell is disrupted. Especially, dispersion, emulsification and particle nanocrystallization of the dendrobium officinale peptidoglycan substances maintain activity of the original substances, solves heat radiation difficulty, and avoids heat sensitivity substances to be destroyed. The technology is mainly applied to batch production of the high-content dendrobium peptidoglycan, and enables the dendrobium polysaccharide to be rapidly absorbed by a human body for prolonging life span.

The present invention is one kind of high activity golden mushroom polysaccharide-peptide- Fe2+ chelate, which has molecular weight of 20-50 KD, and contains polysaccharide in 69.9-70.2 wt%, peptide in 29.3-29.7 wt% and Fe2+ in 0.3-0.6 wt%. The golden mushroom polysaccharide-peptide- Fe2+ chelate is prepared with iron-rich sporophore as material and through the following steps: enzymolyzing with cellulose, hemicellulase or papain to break cell wall to raise the content of effective components, especially polysaccharide; the subsequent degradation of golden mushroom polysaccharide-peptide- Fe2+ chelate under the action of polysaccharidase; and final two-stage ultrafiltering membrane separation to obtain high activity golden mushroom polysaccharide-peptide- Fe2+ chelate with molecular weight of 20-50 KD. The high activity golden mushroom polysaccharide-peptide- Fe2+ chelate can improve systemic immune function, inhibit tumor and replenish iron effectively.

The invention relates to a polysaccharide-peptide with high content and high activity, a preparation method thereof and an application thereof. The polysaccharide-peptide is freely soluble in water; the total amount of glycopeptides is equal to or more than 70 percent; the polysaccharide-peptide with the average weight over 10kd takes up more than 80 percent of the total amount of glycopeptides, wherein, the sugar content is 50 to 80 percent, and the peptide content is 16 to 30 percent; the preparation method is as follows: (1) manyzoned polypore crude extract is made available to be suspended or dissolved in the water at the temperature of 80 to 100 DEG C; the manyzoned polypore crude extract solution is refined and separated, or the hot water with the temperature of 80 to 100 DEG C is used for extracting polyporus versicolor mycelium or manyzoned polypore fruit body for producing the manyzoned polypore crude extract solution; (2) the pH value of the manyzoned polypore crude extract solution or the manyzoned polypore crude extract suspended solution is controlled to be between 4 to 8; the lauter extracting solution can be obtained after filtering or centrifugal separation, which is concentrated to the specific gravity of 1.05 to 1.3; (3) a decoction and alcohol sedimentation method is used for purification. Compared with prior polysaccharide-peptide, the product obtained has more effective anti-tumor effect and better protective effect on marrow function and immune function, which are suitable for preventing and treating tumors.

The present invention provides a polysaccharide peptide standard product, its preparation method and application. It is obtained by using ganoderma lucidum as raw material mother body extract and making said raw material mother body extract undergo the processes of concentration, centrifugalization, dialysis to trap polysaccharide peptide GL-PP with high molecular weight, further separation and purification so as to obtain the invented product. Said standard product can be used for detecting polysaccharide peptide content in ganoderma or ganoderma spore products so as to raise quality of ganoderma products.

By providing a new modern bio-fermentation method for intracellular glycopeptides of Yunzhi, that is, in the production process, continuous feed feeding is adopted and the stirring speed of fermentation is controlled to increase the amount of Yunzhi mycelium, and a new extraction method is adopted at the same time, In order to overcome the problems of low yield of intracellular glycopeptides of Yunzhi in the prior art, etc., the yield and output of intracellular glycopeptides of Yunzhi can be effectively improved. The low-temperature drying process is adopted in the drying step, which overcomes the destruction of the polysaccharide peptide in the intracellular glycopeptide of Versicolor versicolor by the existing high-temperature drying technology, and improves the content of the absorbable polysaccharide peptide, so that it can meet the requirements of the national quality standard and reach the The quality standard of similar products in Japan.

The invention discloses a preparation technology of polygonatum polysaccharide peptide. The polygonatum polysaccharide peptide is prepared from polygonatum by extraction, preliminary purification and separation and purification steps, and has the advantages of high purity, low generate cost, good product quality and three less wastes.

A herbaceous ganoderic polyose is prepared from the herbaceous ganoderma through culturing ganoderma with fungal herb to generate sporophores, washing, drying, crushing, refluxing with alcohol, extracting in hot water, concentrating, depositing in alcohol to obtain the first polyose peptide, washing respectively with alcohol, acetone and ether three times of each, centrifugal separation to removesupernatant, and low-temp drying to obtain crude polyose peptdide. Its advantages are low cost, high extracting rate and high purity.

The invention relates to a method for simply, quickly, directly and accurately measuring the connect of cordyceps sinensis polysaccharide peptide in a cordyceps sinensis product. The method comprises the following steps: performing simple pretreatment on a sample, and qualitatively and quantitatively analyzing through a high performance liquid chromatography photo-diode array (HPLC-PDA) detector. By utilizing the advantages of accurate quantification of high performance liquid chromatography, accurate spectral analysis qualification of the PDA detector and the like, the method can be used for simply, quickly, accurately and directly measuring the connect of cordyceps sinensis polysaccharide peptide in the ordyceps sinensis product, and a simple, reliable and easily-popularized method is supplied to authentication analysis, quality evaluation and quality control of health efficacy factors in the cordyceps sinensis product.

Peptides that mimic the antigenic features of non human-cross-reactive protective epitopes of the MenB CP and nucleic acids encoding the peptide mimetics are disclosed. Antibodies elicited by these peptides do not bind to polysialic acid in host tissue and thus provide a safe and efficacious method for the treatment and/or prevention of Meningitis B.

The invention provides a pig interferon inducer. The active components of the pig interferon inducer consist of purified pig alpha-interferon, ginseng polysaccharide, ginsenoside, hydroxymethyl tuckahoe polysaccharide and polysaccharide-peptide. By weighing up the active components and adding with glycerin and gelatin for fully mixing, a suspending liquid is prepared, and the suspending liquid needs to be stored in light-shading places. For porcine reproductive respiratory syndrome, hog cholera and pseudorabies, the effective prevention rate of the pig interferon inducer provided by the invention is more than 70 percent. The invention provides a veterinary medicine which is safe, effective and low-priced, and can prevent diseases, and the cells are induced to generate the interferon by stimulating an animal body, so that the veterinary medicine has various biological activities of resisting virus in the broad spectrum, improving body immunity and the like, and the aims of preventing and curing viral diseases are achieved. The pig interferon inducer has the advantages of solving the problems of high production cost and inconvenient use existing in the leukocyte interferon and the recombination interferon of the pig, along with simple production process, low production cost and convenient popularization.

The invention discloses a method for extracting abelmoschus esculentus polysaccharide peptide from abelmoschus esculentus. The method comprises the following steps: (1) selecting fresh abelmoschus esculentus fruits and cleanly washing; crushing the abelmoschus esculentus fruits by a crushing machine; (2) heating, reflowing and extracting the crushed abelmoschus esculentus fruits with an ethanol water solution; filtering, and heating, reflowing and extracting filter residues with pure water; filtering; combining filtrates, decompressing and concentrating to obtain liquid 1; (3) carrying out alcohol precipitation with the ethanol water solution; filtering and washing sediment; drying in vacuum and dissolving a dry matter into distilled water to prepare a solution; dialyzing and concentrating dialysate to obtain liquid 2; carrying out alcohol precipitation; filtering and washing sediment with ethanol; drying to obtain raw sugar peptide; and (4) purifying the raw sugar peptide to obtain the abelmoschus esculentus polysaccharide peptide. The method has low production cost; the obtained abelmoschus esculentus polysaccharide peptide has high purity and few three wastes. An anti-saccharifying test shows that the abelmoschus esculentus polysaccharide peptide extracted from abelmoschus esculentus has the anti-saccharifying effect.

The invention provides a method for rapidly determining polysaccharide peptide in a lucid ganoderma product. The method takes a high performance liquid chromatography-evaporative light-scattering detector (HPLC-ELSD) as a detection means and takes lucid ganoderma polysaccharide peptide as a comparison product to rapidly detect the content of the polysaccharide peptide in the lucid ganoderma product. The pre-treatment of a sample only needs simple extraction operation and is convenient and rapid; finally, after the sample is subjected to chromatography high-performance separation, the evaporative light-scattering detector is used for carrying out quantitative analysis on the polysaccharide peptide in the product; a determination result is high in accuracy and good in stability; the method is better than a current common spectrophotometric method, so as to provide a very effective analyzing method for quality identification and quality control of the lucid ganoderma product.

The invention discloses ganoderma active component polysaccharide peptide, a polysaccharide peptide contrast and a polysaccharide peptide separation and purification method. Ganoderma is pulverized, the pulverized ganoderma is soaked with water to obtain a ganoderma water extract, purification treatment is carried out, molecular-weight-level membrane separation is performed, a 10KD dialysis membrane is adopted to intercept a high molecular weight permeable liquid, the permeable liquid undergoes ethanol gradient separation, Bio-GL, and P-10 column chromatographic separation, purification is carried out to obtain an active component polysaccharide peptide contrast, and component identification, taste identification and content determination are carried out in a high-efficiency chromatographic condition. A molecular-weight-level membrane separation is performed to separate a bitter low molecular part. Compared with traditional ethanol extraction, the method is small in risk, high in security, free of toxic or side effect, and easy for industrial production.

The invention provides a rice bran polysaccharide-peptide compound with high oxidation resistance and a preparation method thereof, and belongs to the technical field of rice bran compound preparation, and the method comprises the following steps: 1) taking defatted rice bran as a raw material, mixing the defatted rice bran with water, adding tartaric acid to adjust the pH value, pouring the mixture into a high-pressure homogenizer, conducting homogenizing to obtain a rice bran polysaccharide extracting solution, centrifuging the rice bran polysaccharide extracting solution, and conducting freeze-drying to obtain the rice bran polysaccharide with the relative molecular mass of 10kDa-50kDa; and 2) mixing the rice bran polysaccharide with the rice bran peptide, and carrying out Maillard reaction by adopting a dry method to obtain the polysaccharide-peptide compound. According to the method disclosed by the invention, the polysaccharide-peptide compound with high oxidation resistance can be obtained.

The invention discloses a ganoderan tripeptide filtering system process device. The ganoderan tripeptide filtering system process device is composed of a transporting pump, a stock solution tank, a stop valve, a control valve, a high-pressure pump, a pressure generator, a four-way diverter valve, a series tube, a second four-way diverter valve, a shunt tube, an ultrahigh pressure reaction tank, a polysaccharide peptide storage, a degradation reactor, a refluxing separator, a vacuum pump, a beta-reactor, a protein separator, an ion separator, an ion pump, a homogeneous purifier, a draft tube and a return tube. The ganoderan tripeptide filtering system process device is characterized in that the high-pressure pump and the pressure generator are connected with one end of the refluxing separator through a return tube screw and the other end of the refluxing separator is connected with the screw double-head connector of the high pressure reaction tank through a draft tube screw; a higher pressure than that of the supercritical fluid extracting method is adopted, an ultrahigh pressure is adopted to substitute the supercritical fluid needed temperature cooperation and the damage of the partial substances under high temperature can be avoided; the device is a pure physical extracting process device, the temperature is not higher than 20 DEG C when the ultrahigh pressure is 600 MPa, the low temperature process purifying degree is higher than that of the supercritical fluid extracting method, and the device is an innovative process device for producing the ganoderan peptide injection.

The invention belongs to the technical field of biological extraction, and in particular relates to a preparation method of Guangxi camellia nitidissima polysaccharide peptide. The preparation methodof the Guangxi camellia nitidissima polysaccharide peptide comprises the steps of performing degreasing pretreatment, extracting, performing alcohol precipitation and purifying. The preparation process of the Guangxi camellia nitidissima polysaccharide peptide is reasonable, the extraction efficiency of the of the Guangxi camellia nitidissima polysaccharide peptide is high and the purity is up to86.3 percent; in addition, the prepared polysaccharide peptide has high anti-oxidation activity, the removal rate of hydroxyl free radical reaches to 73 percent, the removal rate of superoxide anion free radical reaches to 81 percent, and theoretical basis is provided for development of the camellia nitidissima serving as anti-oxidation and anti-ageing health-care food.

The invention discloses an extraction method of coriolus versicolor beta-glucan peptide and application of the coriolus versicolor beta-glucan peptide to obesity or related diseases. The extraction method comprises the following steps: washing coriolus versicolor sporophore or spore powder and drying; extracting for 3 times by utilizing water at 100 DEG C, wherein the water which is added for thefirst time is 15 times of coriolus versicolor and the water which is added for the second time is 10 times of the coriolus versicolor; combining extracting solutions of two times; concentrating and adding ethanol to carry out alcohol precipitation; when the alcohol precipitation is carried out for 24 h, filtering and collecting sediment; centrifuging, freezing and drying sediment to obtain a coriolus versicolor polysaccharide crude product; eluting the coriolus versicolor polysaccharide crude product through water, a sodium chloride water solution and the sodium chloride water solution in sequence on a DEAE-Sepharose-fast-flow column; tracking an elution curve by utilizing a sulfuric acid-phenol method, and collecting effective parts obtained by eluting through the sodium chloride water solution and the sodium chloride water solution respectively; concentrating, dialyzing, concentrating and freeze-drying effective partscomponents which are the effective parts components collected and eluted according to the elution curve, so as to obtain a series of beta-glucan peptide. The polysaccharide peptide has the effect of alleviating or controlling the obesity or the related diseases.

The invention relates to a preparation method of low-tyrosine and low-salt flavored soybean paste. The method comprises the following steps of (1) soy sauce residue pretreatment; (2) functional raw material compound formulation; (3) soybean pretreatment; (4) sterilization treatment; (5) flour stir-frying; and (6) preparation of a loofah sponge casein-removed fiber material, wherein the fiber material contains tyrosine aminotransferase; (7) preparation of yeast for making hard liquor; (8) preparation of potassium ion saline water, wherein edible salt, KCl and water are mixed uniformly in a massratio of (1.2-1.5): 0.5: 10; (9) preparation of a W1/O/W2 emulsified bacteria solution; and (10) soybean paste fermentation. Added hericium erinaceus powder, pleurotus eryngii powder, hypsizygus marmoreus powder, cantharellus flocclsus schw powder and tremella powder are rich in polysaccharide, peptide and bioactive substance, and have the effects of helping digestion, benefiting five internal organs, resisting cardiovascular diseases, nourishing the body and the like. In addition, composite mushroom powder is rich in glutamic acid, aspartic acid, pleurotus eryngii polypeptide and the like, and can achieve a natural freshness improving effect.

The invention relates to a nutrition preparation, a preparation method and an application of the nutrition preparation. The nutrition preparation is used for treating refractory pulmonary infection. The nutrition preparation comprises the following ingredients in parts by weight: 35-45 parts of hydrolyzed micromolecular protein powder, 45-60 parts of edible mushroom polysaccharide peptide and 0.73-1.25 parts of vitamin. The nutrition preparation is prepared by mixing the micromolecular protein powder, edible mushroom polysaccharide peptide and the vitamin in proportion. The nutrition preparation can improve nutrition conditions and immunologic functions of a patient with the refractory pulmonary infection.