Method for detecting monosaccharide component in rainbow conk glycopeptide

A technology of versicolor glycopeptide and detection method, which is applied in the directions of measuring devices, material separation, and analysis materials, etc., which can solve problems such as failure to achieve effective separation, and achieve the effect of ensuring quality and ensuring curative effect

Inactive Publication Date: 2013-04-03
SHANGHAI NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

1-Phenyl-3-methyl-5-pyrazolone pre-column derivatization HPLC has been tried for the analysis of monosaccharide components in versicolor glycopeptides, al

Method used

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  • Method for detecting monosaccharide component in rainbow conk glycopeptide
  • Method for detecting monosaccharide component in rainbow conk glycopeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] According to Chinese Pharmacopoeia 2010 Edition (Part One) Appendix VI D high performance liquid chromatography.

[0025] Chromatographic conditions and system suitability test: use Phenomenex Luna C 18 (4.6mm×250mm, 5μm) column; the mobile phase is acetonitrile-phosphate buffer solution, the volume percentage of acetonitrile is 18%, the concentration of phosphate buffer solution is 0.05mol / L, the pH value of phosphate buffer solution is 7.0, UV detection The detector detection wavelength is 255nm, and the number of theoretical plates is 6560 based on the glucose 1-phenyl-3-methyl-5-pyrazolone derivative.

[0026] Preparation of mixed reference solution: Accurately weigh D-glucose, D-mannose, D-xylose, L-fucose, D-galactose, D-arabinose, D-hydrochloric acid dried at 105°C to constant weight Add appropriate amount of glucosamine and L-rhamnose reference substances, add water to prepare a mixed reference solution with a concentration of each monosaccharide of 2mmol / L, ta...

Embodiment 2

[0030] According to Chinese Pharmacopoeia 2010 Edition (Part One) Appendix VI D high performance liquid chromatography.

[0031] Chromatographic conditions and system suitability test: use Hypersil BDS C 18 (4.6mm × 200mm, 5 μm) column; mobile phase is acetonitrile-phosphate buffer, wherein the volume percentage of acetonitrile is 16%, the concentration of phosphate buffer is 0.03mol / L, the pH value of phosphate buffer is 6.9, ultraviolet detection The detector detection wavelength is 250nm, and the number of theoretical plates is 8308 based on the glucose 1-phenyl-3-methyl-5-pyrazolone derivative.

[0032] Preparation of mixed reference substance solution: the same as the preparation method of mixed reference substance standard solution in Example 1.

[0033] Preparation of the test solution: take 10 Yunzhi glycopeptide capsules, pour out the contents and grind them finely in a mortar, accurately weigh 5.0 mg and put them in a 5 mL ampoule, add 1 mL 4mol / L trifluoroacetic ac...

Embodiment 3

[0036] According to Chinese Pharmacopoeia 2010 Edition (Part One) Appendix VI D high performance liquid chromatography.

[0037] Chromatographic conditions and system suitability test: use Phenomenex Gemini C 18 (4.6mm×250mm, 5μm) column; the mobile phase is acetonitrile-phosphate buffer, the volume percentage of acetonitrile is 20%, the concentration of phosphate buffer is 0.07mol / L, and the pH of phosphate buffer is 7.1 , the detection wavelength of the ultraviolet detector is 260nm, and the number of theoretical plates is calculated as 12310 based on the glucose 1-phenyl-3-methyl-5-pyrazolone derivative.

[0038] Preparation of mixed reference substance solution: the same as the preparation method of mixed reference substance standard solution in Example 1.

[0039] Preparation of the test solution: Take 10 pieces of Yunzhi glycopeptide tablets and grind them finely in a mortar, accurately weigh 5.0mg and place them in a 5mL ampoule, add 1mL 2mol / L trifluoroacetic acid and...

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Abstract

The invention belongs to the field of monosaccharide component analysis, and in particular relates to a method for detecting monosaccharide components in rainbow conk glycopeptide. The method comprises the following steps of: carrying out a test on HPLC (High Performance Liquid Chromatography) chromatographic condition and systematic compatibility; subsequently respectively absorbing a mixed reference solution and a test solution to be injected into the HPLC chromatographic instrument so as to obtain a chromatogram, wherein the reference solution is a 1phenyl-3-methyl-5-pyrazolone derivative solution of D-glucose, D-mannose, D-xylose, L-xylose, D-galactose, D-arabinose, D-hydrochloric acid glucosamine and L-rhamnose reference solutions; and the test solution is a 1-phenyl-3-methyl-5-pyrazolone derivative solution of a rainbow conk glycopeptide acid hydrolysis product. Polysaccharide peptide products such as rainbow conk glycopeptide, rainbow conk intracellular glycopeptide, rainbow conk extracellular glycopeptides and coriolus versicolor polysaccharide are distinguished by detecting the mole ratio of eight monosaccharide ingredients in a test sample, so that the quality and the curative effect of the rainbow conk glycopeptide are ensured.

Description

technical field [0001] The invention belongs to the field of analysis of polysaccharide components, and in particular relates to a method for detecting monosaccharide components in versicolor glycopeptides. Background technique [0002] Yunzhi glycopeptide is a Chinese patent medicine extracted from the liquid fermented mycelia of the basidiomycete Yunzhi Cov-1 strain. Its active ingredient is binding proteoglycan. Tumor, anti-oxidation, lowering blood fat, promoting the recovery of damaged liver cells, etc., for esophageal cancer, gastric cancer and primary lung cancer patients with deficiency of both qi and yin and deficiency of heart and spleen caused by radiotherapy and chemotherapy. Yunzhi glycopeptide is a kind of natural macromolecular organic matter with complex and diverse structures. The national standard WS3-228(Z-047)-2003(Z) of Yunzhi Glycopeptides records the main quality control indicators such as the determination of polysaccharide content and protein conten...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 袁萍茅仁刚叶晓平陈浙江林东昊金丹凤吕丹
Owner SHANGHAI NORMAL UNIVERSITY
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