173 results about "D-galactal" patented technology

The present invention relates to methods for modulating the glycosylation profile of recombinantly-expressed proteins. In particular, the present invention relates to methods of controlling the galactosylation profile of recombinantly-expressed proteins by supplementing production medium, e.g., a hydrolysate-based or a chemically defined medium, with manganese and/or D-galactose.

The invention discloses a preparation method for fast separating flavonoid glycosides from oil-tea-cakes with a medium pressure column, which comprises the following steps of: decorticating and crushing camellia oleifera abel seeds, degreasing the camellia oleifera abel seeds with non-polar solvents, performing extraction with ethanol water, filtering and condensing the extract to obtain crude extract concrete, performing fast separation with the medium pressure column to obtain an over 90 percent flavonoid glycoside mixture, and further adopting a high performance liquid chromatography to prepare over 95 percent flavonoid glycoside monomers, wherein the flavonoid glycoside monomers are kaemplerol 3-O-[2-O-beta-D-galactose-6-O-alpha-L-rhamnose]-beta-D-glucoside (I) and kaemplerol 3-O-[2-O-beta-D-xylose-6-O-alpha-L-rhamnose]-beta-D-glucoside (II) respectively. The method can be used for batch preparation, and has the advantage of providing quality raw materials for the development of flavonoid glycoside medicaments and healthcare functional products in the oil-tea-cakes.

This invention relates to a cationically and amphiphilically modified polygalactomannan having repeating units with an average D-mannosyl to D-galactosyl residue ratio of at least 5 to 1 and to compositions containing same. A portion of the hydrogen atoms of hydroxyl groups situated on the mannosyl and galactosyl residues of the galactomannan are replaced with an amphiphilic and a cationic substituent.

The invention belongs to the field of monosaccharide component analysis, and in particular relates to a method for detecting monosaccharide components in rainbow conk glycopeptide. The method comprises the following steps of: carrying out a test on HPLC (High Performance Liquid Chromatography) chromatographic condition and systematic compatibility; subsequently respectively absorbing a mixed reference solution and a test solution to be injected into the HPLC chromatographic instrument so as to obtain a chromatogram, wherein the reference solution is a 1phenyl-3-methyl-5-pyrazolone derivative solution of D-glucose, D-mannose, D-xylose, L-xylose, D-galactose, D-arabinose, D-hydrochloric acid glucosamine and L-rhamnose reference solutions; and the test solution is a 1-phenyl-3-methyl-5-pyrazolone derivative solution of a rainbow conk glycopeptide acid hydrolysis product. Polysaccharide peptide products such as rainbow conk glycopeptide, rainbow conk intracellular glycopeptide, rainbow conk extracellular glycopeptides and coriolus versicolor polysaccharide are distinguished by detecting the mole ratio of eight monosaccharide ingredients in a test sample, so that the quality and the curative effect of the rainbow conk glycopeptide are ensured.

The invention discloses an abelmoschus manihot polysaccharide with an anti-tumor activity and a preparation method thereof. The polysaccharide is formed by beta-D-glucopyranose, alpha-D-mannopyranose, alpha-D-galactose and alpha-L-fucopyranose in a mol ratio of 1: 0.91: 2.14: 1.0, and has the molecular weight of 8867. The structural formula of a basic unit of the polysaccharide is that alpha-D-galactose (1->6) and alpha-D-mannopyranose (2->6) are taken as main chains and mannose C-3 branches are connected with the alpha-L-fucopyranose (1->3) and connected with the beta-D-glucopyranose (->1). According to the abelmoschus manihot polysaccharide disclosed by the invention, an extraction and separation process is selected through a large number of experiments; firstly, a water extraction and alcohol precipitation method is used for obtaining a rough polysaccharide and then protein is removed; and then, a DEAE-52 (Diethyl Aminoethanol-52) cellulose resin and SephadexG-100 gel resin are combined for purifying to prepare the abelmoschus manihot polysaccharide with high purity. Anti-tumor experiment results show that the abelmoschus manihot polysaccharide has very good anti-tumor activity and has no untoward effects after being used for a long period; and the abelmoschus manihot polysaccharide can be conveniently prepared into medicines with various preparations with pharmaceutically acceptable carriers, and is convenient to take clinically.

The invention relates to procedures for the manufacturing of D-galactose and D-galactose containing compositions. More specifically, the invention provides a method for isolating D-galactose from legume material, which method comprises subjecting a legume composition containing non-homologuous soluble polysaccharide, commonly referred to as oligosaccharide, to one or more treatments, resulting in a preparation comprising at least 30% oligosaccharides, and subsequent hydrolysis of the oligosaccharides in this preparation into mainly monosaccharides.

This invention relates to a cationically and hydrophobically modified polygalactomannan having repeating units with a D-mannosyl to D-galactosyl residue ratio of at least 5 to 1 and to compositions containing same. A portion of the hydrogen atoms of hydroxyl groups situated on the mannosyl and galactosyl residues of the galactomannan are replaced with a hydrophobic substituent and a cationic substituent.

The invention discloses a serum free medium for expressing erythropoietin in CHO (Chinese hamster ovary) cells, and a culture method for high expression of erythropoietin in CHO cells. The serum free medium contains additives D-glucose and sodium butyrate, and also contains one or more of D-galactose, D-mannitose and N-acetylglucosamine. The culture method comprises the steps of: performing serumculture on activated seed cells by using a serum medium, and then performing serum free culture by the serum free medium. By adopting the medium and culture method disclosed by the invention, recombinant human erythropoietin protein of high and stable expression can be obtained, and the glycosylation degree of erythropoietin is improved, i.e. the specific gravity of EPO (erythropoietin) sialic acid is improved. As high as 1.0*107IU recombinant human erythropoietin can be obtained from one liter of culture fluid by the culture method provided by the invention, the expression is stable, and theculture method is simple and suitable for large-scale production.

The invention discloses an anti-oxidation active extract of citrus peels as well as an extraction process and application thereof. The active extract of the citrus peels mainly contains a citrus flavonoid substance and pectic polysaccharide. The extraction process of the active extract of the citrus peels comprises the steps of preprocessing raw materials, refluxing and extracting by a dipping method, decompressing and concentrating and drying in vacuum. The invention takes the in-vitro oxidation resistance of the extract to combine with the extraction yield as indexes, adopts a response surface method to optimize the extraction process, has reasonable technological conditions and good effect stability and is suitable for industrialized production. The active extract of the citrus peels, which is obtained by extraction, has an obvious effect to a model mouse with senility caused by D-galactose and can lower the content of the MDA (malondialdehyde) of the plasma, the liver and the brain obviously; the activity of the SOD (superoxide dismutase) of the plasma, the liver and the brain is obviously enhanced; the activity of the GSH-Px (glutathione peroxidase) of the plasma and the liver is obviously enhanced; and the invention can be used for preparing an anti-oxidation medicine, an anti-oxidation food or an anti-oxidation health-care product.

The invention discloses a preparation method of polysaccharide in pine cone from Pinus koraiensis, relating to a preparation method of polysaccharide in pine cone from Pinus koraiensis. The invention solves the problems that the traditional preparation method of the polysaccharide in pine cone from Pinus koraiensis cannot completely remove chemical components with weak polarity and medium polarity in the pine cone from Pinus koraiensis and the protein removal rate is relatively lower. The preparation method comprises the following steps: 1. degreasing; 2. extracting; 3. preparing coarse polysaccharide; 4. deproteinizing; and 5. decoloring, collecting precipitates, freezing and drying to obtain the refined polysaccharide in pine cone from Pinus koraiensis. The extraction rate of the neutral polysaccharide in pine cone from Pinus koraiensis prepared by the invention can reach 1.15-1.52 percent, the protein removal rate is up to over 70 percent and the pigment removal rate is up to over 95 percent. The chromatography test shows that the neutral polysaccharide mainly comprises seven monosaccharides, namely D-ribose, L-rhamnose, L-arabinose, D-xylose, D-mannose, D-glucose and D-galactose, and the preparation method of polysaccharide in pine cone from Pinus koraiensis is applied to the field of preparation of neutral polysaccharide pine cone from Pinus koraiensis.

The invention discloses a method for extracting L-arabinose and D-galactose from Arabic gum, which comprises the following steps of: (1) performing catalytic hydrolysis on the Arabic gum by using an acid catalyst A1, neutralizing, and evaporating out moisture from the obtained reaction liquid to obtain mixed thick syrup; (2) adding an acid catalyst A2 and ketone compounds into the mixed thick syrup for etherification reaction, and after the reaction is finished, neutralizing, distilling to recover the ketone compounds, dissolving the residue by using a mixture of alcohol compounds and water, and performing extraction treatment to respectively obtain arabinose etherate and galactose etherate; and (3) dehydrating and de-etherizing the obtained arabinose etherate and galactose etherate under the catalysis of an acid catalyst A3, neutralizing, and performing decoloration, drying by distillation and crystallization on the obtained solution to obtain the L-arabinose and the D-galactose. The method has the advantages of simple operation, high quality and yield of products, low production cost, capacity of recycling a solvent, suitability for large-scale industrial production and the like.

The invention discloses application of ethyl asterrate in preparation of an anti-alzheimer medicine, and in particular relates to application of diphenyl ether compound ethyl asterrate shown in the structural formula (I) in inhibition of the activity of acetylcholine esterase and preparation of an anti-alzheimer medicine. Experiments prove that the ethyl asterrate can remarkably inhibit the activity of acetylcholine esterase, can remarkably improve the memory and space exploration capability of an alzheimer rat model caused by D-galactose and AlCl3, can be applied to preparation of the anti-alzheimer medicine, and indicates an excellent market application prospect.

A monosaccharide production system is disclosed. The production system can be directed to processes for producing a D-galactose preparation, a D-galactose preparation and an isoflavones preparation, a tagatose preparation, and a tagatose preparation and an isoflavones preparation.

The invention relates to a method for identifying stigma maydis polysaccharide by utilizing thin layer chromatography and belongs to the technical field of medicine authentication. The method comprises the following steps that: a thin layer plate is prepared by silica gel G and sodium carboxymethylcellulose (CMC-Na); D-glucose, D-xylose, D-mannose, L-Arabinose, Alfa-rhamnose, D-galactose and D-galacturonic acid are used as reference substances; corn stigma polysaccharide crude product extracted by water extraction and alcohol precipitation is used as a sample, the sample is dissolved by 50% ethanol, trifluoroacetic acid is utilized to carry out hydrolysis, hydrogen peroxide is used for decoloration, normal butanol methanol, chloroform, glacial acetic acid and water are used as developing solvents, and aniline, diphenylamine, phosphoric acid and water solution are used for color development. The spot position and color of the sample and the reference substances are consistent, so that the type of saccharine contained in the corn stigma can be judged. The method has high specificity, is simple to operate and can be used for accurately identifying the type of monosaccharide in the stigma maydis polysaccharide.

The invention provides a neoagarobiose hydrolase. The amino acid sequence of the neoagarobiose hydrolase is SEQ IDNO:1. The neoagarobiose hydrolase can degrade neoagarobiose to generate 3,6-anhydro-L-galactose (L-AHG) and D-galactose, and therefore the neoagarobiose hydrolase can be used for preparing pure L-3,6-inner ether galactose. Serving as a biocatalyst used for producing the L-AHG, the neoagarobiose hydrolase has a user-friendly reaction environment, high catalytic activity, a certain advantage over a chemical method and good application potential and prospects. The L-AHG is a kind of levoglucosan, has good skin whitening performance and anti-inflammatory activity and has very good potential application value in the aspects of medicine and cosmetics. According to recombined escherichia coli strains, recombined agarase expressed by pETNA/BL21 accounts for 28% of total protein. The expression level is high, the recombined agarase is easily purified and expression quantity is not reduced after continuous passage is carried out. The neoagarobiose hydrolase is adopted for preparing recombined neoagarobiose hydrolase and the L-AHG in a mass mode.

The invention discloses a method for extracting xylitol and D-galactitol from xylose mother solution, which adopts a homologous exchange method to construct a bacillus subtilis 168 xylose isomerase gene (xylA) deficient strain Bsxyl, utilizes the engineering strain Bsxyl to purify xylose mother solution to obtain xylose and D-galactose and further carries out catalytic hydrogenation and crystallization separation to obtain xylitol and galactitol. The Bsxyl strain is already stored in the China General Microbiological Culture Collection Center on March 25, 2010, the strain accession number of the Bsxyl strain is CGMCC No.3692, and the strain can purify xylose mother solution to obtain xylose and D-galactose. The strain can purify fermentation culture solution containing xylose mother solution, so that the total purity of xylose and galactose can reach more than 88.8 percent, and after further catalytic hydrogenation and crystallization separation, obtaining xylitol and D-galactitol the purity of which is more than 98 percent.

The invention discloses hawthorn fruit cyanidin-3-hydroxyl glycosyl transferase and encoding genes and application thereof. The invention provides protein. The protein is protein as shown in sequence 2 in a sequence table or protein derived from the sequence 2. The protein derived from the sequence 2 has the same function of the protein as shown in the sequence 2 and is obtained by subjecting the amino acid sequence as shown in the sequence 2 in the sequence table to one or more amino acid residue substitution and/or loss and/or adding. Hawthorn fruit cyanidin-3-O-galactoside transferase (Cp3OGT) genes are cloned from hawthorn fruit for the first time. In-vitro experiments show that Cp3OGT has the activity for catalyzing cyanidin and UDP-D-galactose to generate cyanidin-3-O-galactoside.

The invention provides a method for establishing an animal model of an Alzheimer's disease. The method includes feeding a rat for at least 60 days under the condition of exposing to a power-frequency magnetic field; feeding the rat with drug D of galactose and beta of amyloid protein liquid during the process of feeding, and accordingly obtaining the animal model of the Alzheimer's disease. Thus, the method can be a useful supplement of a single-factor-induced animal model of the Alzheimer's disease. According to an embodiment of the method, characteristic pathological changes of the animal of the Alzheimer's disease can be expressed; the method has a characteristic of learning and memory impairment, is consistent with the environmental requirements of long-time and lasting exposure to the power-frequency magnetic field, and has the advantages of stable performance, good reproducibility, and low cost.