Method for extracting xylitol and D-galactitol from xylose mother solution

A technology for preservation of xylose and strains, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of reducing corporate profits, high viscosity, complex components, etc.

Inactive Publication Date: 2010-10-27
GUANGXI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its high sugar concentration, its viscosity is very high, and its composition is relatively complex. In addition to the above-mentioned monosaccharides, it also contains a large amount of pigments and colloids (Applied Chemical Industry, 38(1): 8-10.2009), which can no longer pass the current crystallization process. Technology to separate the xylose in it
Therefore, at present, major manufacturers are selling at low prices, which greatly reduces the profits of enterprises.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Inoculate the engineered strain BSxyl into 10ml LB and cultivate overnight to be the primary seed, transfer 5ml of the primary seed to 50ml fermentation medium (12% xylose mother liquor, 1.0% yeast powder, 0.5% peptone, pH6.5) and culture at 200rpm Obtain secondary seeds overnight, inoculate all 50 ml of secondary seeds into 450 ml of the same fermentation medium, culture at 200 rpm for 48 hours, and take samples to detect the content and purity of xylose. Calculated by combining xylose and D-galactose, after 48 hours of purification, the metabolism of L-arabinose and glucose is incomplete, and the total purity of xylose and galactose in the fermentation broth reaches 70.6%.

Embodiment 2

[0013] Inoculate the engineered strain BSxyl into 10ml LB and cultivate overnight to be the primary seed, transfer 5ml of the primary seed to 50ml fermentation medium (12% xylose mother liquor, 1.0% yeast powder, 0.5% peptone, pH6.5) and culture at 200rpm Obtain secondary seeds overnight, inoculate all 50 ml of secondary seeds into 450 ml of the same fermentation medium, culture at 200 rpm for 60 hours, and take samples to detect the content and purity of xylose. Calculated by combining xylose and D-galactose, after 60 hours of purification, both L-arabinose and glucose are completely metabolized, and the total purity of xylose and galactose in the fermentation broth reaches 88.8%.

Embodiment 3

[0015] Inoculate the engineered strain BSxyl into 10ml LB and cultivate overnight to be the primary seed, transfer 5ml of the primary seed to 50ml fermentation medium (12% xylose mother liquor, 1.0% yeast powder, 0.5% peptone, pH6.5) and culture at 200rpm Obtain secondary seeds overnight, inoculate all 50 ml of secondary seeds into 450 ml of the same fermentation medium, culture at 200 rpm for 72 hours, and take samples to detect the content and purity of xylose. Calculated by combining xylose and D-galactose, after 72 hours of purification, both L-arabinose and glucose were completely metabolized, and the total purity of xylose and galactose in the fermentation broth reached 89.2%.

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Abstract

The invention discloses a method for extracting xylitol and D-galactitol from xylose mother solution, which adopts a homologous exchange method to construct a bacillus subtilis 168 xylose isomerase gene (xylA) deficient strain Bsxyl, utilizes the engineering strain Bsxyl to purify xylose mother solution to obtain xylose and D-galactose and further carries out catalytic hydrogenation and crystallization separation to obtain xylitol and galactitol. The Bsxyl strain is already stored in the China General Microbiological Culture Collection Center on March 25, 2010, the strain accession number of the Bsxyl strain is CGMCC No.3692, and the strain can purify xylose mother solution to obtain xylose and D-galactose. The strain can purify fermentation culture solution containing xylose mother solution, so that the total purity of xylose and galactose can reach more than 88.8 percent, and after further catalytic hydrogenation and crystallization separation, obtaining xylitol and D-galactitol the purity of which is more than 98 percent.

Description

technical field [0001] The invention relates to a method for preparing sugar alcohols in the field of biotechnology, in particular to obtaining xylose and D-semi Lactose provides a convenient and effective method for purifying xylose and D-galactose from xylose mother liquor to further generate xylitol and D-galactitol. Background technique [0002] The acid hydrolyzate of bagasse and corncob undergoes purification processes such as neutralization, decolorization, ion exchange, and concentration to obtain a xylose-rich massecuite, and the tea-brown viscous liquid left after the xylose is separated by gradient cooling crystallization is the xylose mother liquor. On average, one ton of xylose is obtained for every ton of xylose separated by crystallization. At present, there are more than ten xylose manufacturing enterprises in my country, with an annual output of about 50,000 tons of xylose, that is, about 50,000 tons of xylose mother liquor. Xylose mother liquor is rich in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12P19/02C12R1/125
Inventor 姜明国杨立芳王犇
Owner GUANGXI UNIV FOR NATITIES
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