36 results about "Tagatose" patented technology

Disclosed herein are formulations (including sweet proteins and rare sugars) for improving the sweetness of low glycemic index rare sugars without altering the texture and flavor of the rare sugars and methods for improving the sweetness of rare sugars. More specifically, the present invention discloses methods for improving the sweetness of rare sugars without altering the sweetness profile, texture and flavor of the primary sugars. The invention also discloses the addition of Brazitame protein with liquid rare sugars at different temperatures during the synthesis of the rare sugars, before crystallization or before or after caramelization of the rare sugars, to increase sweetness. The Brazilian sweet protein increases the sweetness of rare sugars, but is not limited to D-psicose, D-tagatose, D-allose, isomaltulose, trehalose, sorbose, pinose, leucobiose and the like. The invention also provides for the evaluation of non-nutritional sweeteners (such as aspartame, acesulfame, sodium cyclamate, neotame, saccharin, sucralose, stevioside, etc.), as each has a limitation of taste quality if used with rare sugars. However, the use of Brazilian sweet protein does not alter the flavor and texture of rare sugars, and the taste profile matches sucrose.

The present invention relates to a use of an enzyme belonging to the aldolase group and comprising a polypeptide having at least 30% identity with an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 4, for stereoselectively preparing D-tagatose-1,6-bisphosphate (TBP) or D-tagatose-1-phosphate. The present invention also relates to a method for preparing D-tagatose-1,6-bisphosphate (TBP) or D-tagatose-1-phosphate, comprising a step of reacting, in a suitable reaction medium, dihydroxyacetone phosphate (DHAP) or at least an isomer thereof or analog thereof, and optionally D-glyceraldehyde, with an enzyme belonging to the aldolase group, said enzyme comprising a polypeptide having at least 30% identity with an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 4. Finally, the present invention relates to a process for preparing D-tagatose.

To provide an immobilized enzyme composition for hexose production or an improved process for hexose production. [Solution] The present invention relates to an immobilized enzyme composition for the preparation of hexoses. Examples of hexoses include tagatose, psicose, fructose, allose, mannose, galactose, altrose, talose, sorbose, gross, idose, and inositol. The present invention also relates to an enzymatic process for preparing hexoses from sugars by contacting starch derivatives with the immobilized enzyme composition of the present invention.

The utility model discloses an ion exchange device for tagatose production, which relates to the technical field of ion exchange and comprises a base, a fixing support is fixedly connected to the upper end of the base, and four fixing rods are fixedly connected to the side wall of the fixing support. The end, away from the fixing support, of the fixing rod is fixedly connected with a first exchange assembly, a second exchange assembly, a third exchange assembly and a fourth exchange assembly respectively, and the first exchange assembly, the second exchange assembly, the third exchange assembly and the fourth exchange assembly each comprise an installation cylinder. The position of the inner cylinder in the mounting cylinder can be conveniently adjusted, so that the height of the whole ion exchange assembly can be flexibly adjusted, the device can meet the mounting requirements of equipment with different heights, the applicability and the flexibility of the device are greatly enhanced, and in the mounting or dismounting process, only the limiting cylinder needs to be simply rotated, and the mounting and dismounting are convenient. Therefore, the pipeline can be quickly spliced with or detached from the liquid outlet at the lower end of the mounting cylinder and the liquid inlet at the upper end of the inner cylinder.

The present application relates to the technical field of biotechnology, and particularly relates to a tagatose-6-phosphate phosphatase mutant and a method for synthesizing tagatose from scratch. The method provided by the present application is a one-pot method for biosynthesizing D-tagatose by using starch or dextrin as a substrate, adopting a multi-enzyme cascade reaction system composed of a tagatose-6-phosphate phosphatase mutant, a glycogen-debranching enzyme, an alpha-glucan phosphorylase, a phosphoglucomutase, a glucose-6-phosphate isomerase, a D-tagatose-6-phosphate-3-epimerase and a 4-alpha-glucan transferase. The method can prepare tagatose from ordinary and inexpensive starting materials (starch or dextrin) at a high yield, improves the overall conversion rate of the system and the final yield of tagatose, provides a new technical approach for realizing low-cost and green production of tagatose, and has the advantages of high yield, low cost and being more suitable for industrial production.

This invention belongs to the field of biosynthesis technology and relates to a method for producing D-tagatose under nickel-free conditions and its application. The method includes: mixing tagatose 4-epimerase with a substrate solution, reacting the mixture, and collecting the supernatant by centrifugation to obtain the product. The substrate solution is prepared from D-fructose, glycine, sodium hydroxide, and water. This invention uses tagatose 4-epimerase to catalyze the production of D-tagatose, and the reaction system does not contain nickel ions or other heavy metal ions, thus avoiding the safety hazards caused by nickel residues. This method has a wider range of applications and higher safety.

The invention discloses a method for producing D-tagatose with high efficiency and low cost. According to the invention, a double-enzyme cascade engineering strain B.subtilis WB800N-pHT01-araA-lacZ, which can be used for simultaneously expressing a beta-galactosidase gene lacZ and an L-arabinose isomerase gene araA, is constructed, and the double-enzyme cascade engineering strain B.subtilis WB800N-pHT01-araA-lacZ is constructed; culturing the engineering strain, centrifugally collecting thalli, resuspending the collected thalli by using a substrate solution containing whey powder (lactose), and reacting at constant temperature; and finally, centrifuging the reaction liquid after the reaction is finished, taking supernate, filtering the supernate through a 0.22 m water system small filter, and determining the D-tagatose in the supernate by high performance liquid chromatography, the yield of the D-tagatose being up to 77.5 g / L. The method provided by the invention can realize efficient reutilization of the milk product byproduct whey powder, and has the advantages of simple operation method, mild reaction conditions, high efficiency, low cost and the like.

The present invention relates to a process for preparing tagatose from a biomass or any carbohydrate source derived sugar. The present invention includes methods for producing tagatose using a range of enzymes to convert biomass, such as, but not limited to, starch, maltose, maltodextrin, cellulose, sucrose from juice, and monosaccharides, such as, but not limited to, glucose and / or fructose, etc. The invention also focuses on the use of enzymes alone and / or fused with enzymes involved in successive steps for the economical, efficient conversion of biomass sugars into tagatose.

The invention discloses recombinant escherichia coli for producing D-tagatose by using redox enzyme driven by cofactor regeneration as well as a construction method and application of the recombinant escherichia coli, and belongs to the technical field of biological engineering. The method comprises the following steps: introducing xylose reductase xyrB, glucose dehydrogenase Gox2015, galactitol dehydrogenase RlGDH mutant T193G / G98C and water-producing NADH oxidase SpNox into escherichia coli, so as to construct recombinant escherichia coli; according to the method disclosed by the invention, a way for synthesizing the D-tagatose by regenerating and driving oxidoreductase through cofactors is designed and constructed, and lactose in whey can be completely converted into the D-tagatose and sodium gluconate by combining the way with beta-galactosidase, so that high-valued utilization of a dairy product processing by-product whey is realized.

The invention discloses a D-tagatose-3-epimerase mutant and application thereof, the D-tagatose-3-epimerase mutant has a single-point mutation or a multi-point mutation in the 45th mutation, the 48th mutation, the 50th mutation, the 80th mutation, the 93th mutation, the 98th mutation and the 144th mutation relative to an amino acid sequence SEQ ID NO.1 of a wild type D-tagatose-3-epimerase, and the D-tagatose-3-epimerase mutant has a single-point mutation or a multi-point mutation in the 45th mutation, the 48th mutation, the 50th mutation, the 80th mutation, the 93th mutation, the 98th mutation and the 144th mutation. The invention also provides a corresponding coding gene, a recombinant vector, a genetically engineered bacterium, a biocatalyst and application in preparation of rare sugars. The invention develops the D-tagatose-3-epimerase mutant which has stronger adaptability with glucose isomerase and is more stable in a cascade reaction, is beneficial to improving the efficiency of reaction for preparing rare sugar by using xylose, can simultaneously consider higher substrate concentration and D-xylose conversion rate, and reduces the production cost of the product.

The invention discloses bread capable of regulating and controlling protein accumulation behaviors through tagatose coupled vital gluten and biologically modified dietary fibers to achieve low GI and a preparation method of the bread, and relates to the technical field of baking. Kneading dough; making dough; performing fermentation; performing baking; the raw materials of the compound functional sugar substitute bread not containing the butter are evenly mixed, water is added, the mixture is kneaded into dough, the dough is whipped, the whipping is divided into high-speed whipping and low-speed whipping, and the tagatose is coupled with the vital gluten and the biologically modified dietary fibers to regulate and control the protein accumulation behavior, so that the low-GI bread is achieved. By replacing 60% or more of cane sugar with tagatose, replacing 15% or more of high-gluten flour with biologically modified dietary fiber and replacing 7% or more of high-gluten flour with vital gluten for bread making, the bread made by the method has high specific volume and good flavor, the use amount of cane sugar in bread making is greatly reduced, and the bread is more suitable for bread making. And the use amount of the high-gluten wheat flour is reduced to a certain extent.

The invention discloses a tagatose-4-epimerase and an application of the tagatose-4-epimerase in preparation of D-tagatose. The amino acid sequence of the tagatose-4-epimerase comprises a sequence as shown in SEQ ID NO. 1 to SEQ ID NO. 9. The invention also discloses a preparation method of the tagatose-4-epimerase. The tagatose-4-epimerase disclosed by the invention is used as a catalyst to catalyze D-fructose, and the yield of D-tagatose can reach more than 30%.

Disclosed are components and methods for preparing tagatose from galactose via isomerization reactions using engineered components. The engineered components include microbial cells and methods for preparing microbial cells that have been engineered to catalyze isomerization of galactose to tagatose, in which the microbial cells express cytoplasmically an exogenous L-arabinose isomerase enzyme. The disclosed microbial cells may further be modified for use in methods for preparing tagatose from galactose via isomerization reactions where the microbial cells are treated with reagents that permeabilize the cells. The disclosed methods enable isomerization reactions of galactose to tagatose at relatively high rates, high conversions, and elevated temperatures.

This invention discloses a strain for the efficient synthesis of D-tagatose, its preparation method, and its applications. Through strategies such as the discovery of novel galactitol dehydrogenase and promoter engineering, this invention initially increased tagatose yield by 28.48%. Simultaneously, a highly efficient and sensitive tagatose biosensor was developed, which was used as a screening tool for xylose reductase. Ss XR molecularly modified the strain to obtain the F128M-Q219K double mutant, which achieved a tagatose yield of 5.39 g / L in the producing strain. Based on this, amino acid complementation optimization was performed on the high-yielding strain, and the final engineered strain achieved a tagatose yield of 44.61 g / L in a 5 L bioreactor. This study provides a new strategy and core technology support for the efficient biosynthesis of tagatose.

This application discloses a mouthwash and its preparation method, belonging to the field of mouthwash. The mouthwash includes a composition comprising a main filler and an active substance; the main filler includes tagatose; by mass parts, the main filler is 40-90 parts and the active substance is 0.1-10 parts. In this application, by setting the main filler to 40-90 parts, and by including tagatose, the mouthwash contains a relatively high amount of tagatose, ensuring that the mouthwash is less susceptible to moisture absorption during storage, thereby improving the mouthwash's taste.

The application discloses a recombinant domain fusion enzyme and application thereof in preparation of D-allulose, wherein the recombinant domain fusion enzyme is formed by splicing and recombining a 5' domain and a partial 3' domain of D-tagatose 3-epimerase and an active center domain of xylose isomerase. The application uses the domain fusion enzyme of the 5' domain and the partial 3' domain of D-tagatose 3-epimerase and the active center domain of xylose isomerase as a biological catalyst, and the yield of D-allulose generated by catalyzing isomerization of 260 g / L glucose is 35.9%. The method disclosed by the application is rapid and efficient, reaction conditions are simple, substrate conversion rate is high, and byproducts are few, so that the pressure of separation and purification is reduced, and the method is suitable for large-scale industrial production.

The application discloses an engineered bacillus subtilis strain for producing tagatose, a construction method and application thereof, and belongs to the technical field of bioengineering. The engineered strain takes bacillus subtilis as a host, co-expresses L-arabinose isomerase from Erysipelothrix larvae and beta-galactosidase from escherichia coli, and constructs the bacillus subtilis for co-expressing double enzymes through ribosome binding sites or promoters in series. The double-enzyme bacillus subtilis whole cell catalyzes lactose to produce D-tagatose, and the yield and conversion efficiency of D-tagatose are significantly improved after optimization of the enzymatic reaction. The application adopts single-strain double-enzyme co-expression and one-step whole-cell catalysis, uses low-cost lactose as a substrate, and has the advantages of mild reaction, less by-product, simplified process, no endotoxin safety hazard, and the like. The application solves the problems of low conversion rate, high substrate cost and complicated strain compounding in the prior art, and is suitable for industrialized production of food-grade D-tagatose.

The application discloses a tagatose 4-epimerase mutant with improved catalytic efficiency and application thereof. Thermoproteales archaeon The tagatose 4-epimerase mutant is obtained by replacing the serine at the 46th position with alanine in the amino acid sequence of tagatose 4-epimerase from The tagatose 4-epimerase mutant has significantly improved catalytic activity compared with the wild type, and the catalytic efficiency and yield of the mutant in a whole cell system are improved, which indicates that the recombinant mutant bacteria have good industrial application potential.

The invention discloses preparation and application of a phosphoric-acid-dependence-free tagatose-diphosphate aldolase mutant, and belongs to the field of gene engineering and enzyme engineering. The tagatose-diphosphate aldolase KoT4E gene derived from Ko is expressed in E. coli BL21 (DE3), the tagatose-diphosphate aldolase is applied to a reaction system with d-fructose as a substrate, enzyme reaction is carried out under the conditions that the pH value is 8.5 and the temperature is 70 DEG C, and the yield of the d-fructose after reaction is carried out for 12 hours reaches 140.5 g / L. The recombinant strain is subjected to high-density fermentation, a crude enzyme liquid obtained after cell wall breaking of thallus cells is added into a 1.5 L large reaction system, the yield of d-tagatose can reach 126.75 g / L after the reaction is conducted for 12 h, and industrial production of d-tagatose can be achieved.

This invention provides a compound composition for regulating glucose and lipid metabolism in diabetes and its application, belonging to the fields of medicine, food, and health products. The composition comprises an enzymatically hydrolyzed extract of Polygonatum sibiricum, a compound extract of traditional Chinese medicine including Astragalus membranaceus, ginseng, mulberry leaf, cinnamon, white kidney bean, and bitter melon, probiotics including Lactobacillus rhamnosus and Bifidobacterium bifidum B11, hydrolyzed collagen peptides, and tagatose, scientifically formulated according to the principles of traditional Chinese medicine. This composition can comprehensively regulate the multidimensional pathological characteristics of diabetes, such as glucose and lipid metabolism disorders, chronic inflammation, and weight loss, exerting a synergistic effect. Its hypoglycemic, lipid-regulating, anti-inflammatory, and weight-recovery-promoting effects are significantly better than the sum of the individual effects of each component. It can also significantly improve serum free fatty acid, fasting insulin, and inflammatory factor levels, providing a new approach for developing safe and effective adjunctive treatment products for diabetes.

The invention discloses a composite plant source and functional sugar powder composition for assisting in reducing blood sugar. The composition is prepared from the following raw materials in parts by weight: 5 to 10 parts of stevia rebaudiana polyphenol, 8 to 15 parts of angelica keiskei extract, 10 to 20 parts of aronia melanocarpa fruit extract, 15 to 25 parts of lithocarpus litseifolius extract, 3 to 8 parts of tagatose, 15 to 20 parts of oat beta-glucan, 20 to 30 parts of resistant dextrin, 2 to 5 parts of L-arabinose, 50 to 100 parts of a drying aid, 1 to 3 parts of a flow aid and 2 to 5 parts of a stabilizer. The powder is prepared through targeted extraction process optimization (55% ethanol aqueous solution extraction of stevia rebaudiana polyphenol, ultrasonic-assisted water extraction of angelica keiskei, acidic ethanol extraction of aronia melanocarpa fruits and composite enzymolysis-membrane separation of lithocarpus litseifolius) and low-temperature spray drying, and the retention rate of active ingredients is greater than or equal to 92%; experiments show that the powder can enable the blood glucose reduction amplitude of high-glucose diet mice to reach 38%-46% within 2 h after meal, the dissolution speed is smaller than or equal to 30 s, and the powder is free of gastrointestinal tract side effects and suitable for being brewed and drunk by people with hyperglycemia or added into food to serve as a health care product for assisting in reducing blood glucose.

A composition includes a sugar source and inositol. The sugar source is one more sugars select from the group consisting of glucose, sucrose, sucralose, tagatose, galactose, high fructose corn syrup, fructose, isoglucose, and rhamnose. The composition of sugar and inositol has an unique properties that prevents or limits the signaling of TNF-α and associated pro-inflammatory cytokines when metabolized by an individual consuming the composition. Accordingly, the composition can be advantageously used to control blood glucose levels, treat diabetes and related conditions as well as treat diseases based on an inflammatory response.

The present invention relates to a use of an enzyme belonging to the aldolase group and comprising a polypeptide having at least 30% identity with an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 4, for stereoselectively preparing D-tagatose-1,6-bisphosphate (TBP) or D-tagatose-1-phosphate. The present invention also relates to a method for preparing D-tagatose-1,6-bisphosphate (TBP) or D-tagatose-1-phosphate, comprising a step of reacting, in a suitable reaction medium, dihydroxyacetone phosphate (DHAP) or at least an isomer thereof or analog thereof, and optionally D-glyceraldehyde, with an enzyme belonging to the aldolase group, said enzyme comprising a polypeptide having at least 30% identity with an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 4. Finally, the present invention relates to a process for preparing D-tagatose.

The invention discloses a tagatose-6-phosphate phosphatase mutant and application of the tagatose-6-phosphate phosphatase mutant in production of D-tagatose, and belongs to the technical field of enzyme engineering. The tagatose-6-phosphate phosphatase mutant disclosed by the invention is obtained by mutating wild type tagatose-6-phosphate phosphatase of which the amino acid sequence is shown as SEQ ID NO.5, and the mutation site is selected from at least one of the 45th site, the 108th site, the 168th site and the 186th site. After the mutant is coupled with isoamylase, dextran phosphorylase, glucose phosphate mutase, glucose-6-phosphate isomerase, 4-alpha-glucosyltransferase and tagatose-6-phosphate isomerase, the yield of D-tagatose synthesized by substrate starch is increased, the accumulation amount of by-products glucose and fructose is low, and the yield of D-tagatose synthesized by substrate starch is increased. Therefore, the production efficiency is greatly improved, and the separation and purification cost is reduced. Therefore, the mutant disclosed by the invention is suitable for industrial production and has a good application prospect.

The invention discloses a zero-sugar lipid-lowering instant cold-brewed tea beverage containing psicose and a preparation method of the zero-sugar lipid-lowering instant cold-brewed tea beverage, and belongs to the technical field of foods. The zero-sugar lipid-lowering instant cold-brewed tea is prepared from the following raw materials in parts by weight: 15-25 parts of mulberry leaves, 15-25 parts of bitter gourds, 10-16 parts of coix seeds, 10-16 parts of lotus leaves, 10-20 parts of haws, 5-7 parts of polygonum cuspidatum, 10-20 parts of psicose and 1-3 parts of tagatose. By taking psicose-tagatose isomeric bridging as a core and cooperating with a low-temperature freeze-thawing-variable-temperature differential-pressure puffing-vacuum freeze-drying three-in-one technology, three major pain points of hot brewing limitation, bitter taste and dark color and active ingredient volatile of a traditional tea drink are broken through at one stroke, and instant dissolution in cold water less than or equal to 5 seconds, zero sugar, transparent color, elegant fragrance and refreshing taste are realized; the health-care food can be soaked in cold water, can be instantly dissolved, and has the effects of scientifically reducing blood sugar and easily controlling body.

The application discloses a D-tagatose 4-epimerase mutant for efficiently synthesizing D-tagatose and application thereof. The wild-type D-tagatose 4-epimerase is screened first, and the wild-type enzyme capable of being used for mutation to further improve activity is obtained. The wild-type enzyme is then subjected to mutation and modification, and a plurality of high-temperature-resistant D-tagatose 4-epimerase mutants are screened, so that the conversion efficiency of the mutants in catalyzing D-fructose to synthesize D-tagatose is improved. The conversion rate of the obtained mutants at 70 DEG C is more than 3 times of that of the wild type, and the mutants can catalyze higher-concentration substrates to be converted compared with the wild type.

The utility model relates to the technical field of tagatose production equipment, discloses a tagatose crystallization device, and solves the problems that materials are not uniformly mixed, the temperature is difficult to regulate and control, crystallized materials are easy to remain and accumulate and impurities are easy to mix in the existing tagatose crystallization process. The device comprises a mixing tank, a metering and feeding mechanism with a driving motor and auger blades at the top end, a stirring and scraping assembly driven by a low-speed motor, a temperature control structure with a double-layer jacket and a spiral flow guide pipe and other key components. The metering and feeding mechanism realizes accurate feeding of seed crystals and a stock solution by virtue of accurate driving and conveying design, and promotes rapid and uniform mixing of raw materials in cooperation with the strong stirring efficiency of a double-helix ribbon in the stirring and scraping assembly. Meanwhile, a unique double-layer jacket of the mixing tank is matched with a spiral flow guide pipe, so that the internal thermal environment is precisely regulated and controlled; the cap-shaped filter screen is tightly cooperated with the matched scraper blade and scraper, so that impurities are effectively blocked, and the filter screen is cleaned in time.

The utility model discloses a stirring structure of a tagatose fermentation device, which relates to the technical field of tagatose fermentation and comprises a fermentation tank, a feed hopper is arranged at the top end of the fermentation tank, a cooling and stirring mechanism comprises a pair of rotating pipes arranged in the fermentation tank, and the top ends and the bottom ends of the rotating pipes respectively and rotatably penetrate through the top end and the bottom end of the fermentation tank. A plurality of groups of stirring blades are uniformly and fixedly mounted on the pair of rotating pipes at equal intervals, the plurality of groups of stirring blades on the pair of rotating pipes are respectively arranged in a staggered manner, cooling cavities are formed in the stirring blades, and the cooling cavities are communicated with the rotating pipes. Through the arrangement of the cooling and stirring mechanism, external liquid enters the rotating pipe and is conveyed into the cooling cavity in the stirring blade at the same time, materials are stirred when the rotating pipe drives the stirring blade to rotate, the materials are cooled through the rotating pipe and the liquid in the cooling cavity, heat generated in the stirring process is absorbed, and the stirring effect is improved. The influence of heat on the fermentation of the tagatose is avoided.

Provided are a preparation method for an immobilized cell for tagatose production and a method for producing tagatose by using the immobilized cell. The preparation method for the immobilized cell comprises: mixing a fermentation broth of Escherichia coli or Bacillus subtilis that expresses α-glucan phosphorylase, phosphoglucomutase, glucose phosphate isomerase, tagatose 6-phosphate epimerase, and tagatose 6-phosphate phosphatase to obtain a fermentation mixture, adding inorganic soil and then performing uniform stirring, then adding a flocculant to flocculate bacteria, subsequently adding a cross-linking agent to cross-link, performing vacuum filtration to obtain a filter cake, using a rotary granulator to extrude the filter cake to granulate into a long strip, then cutting by means of a spherical shot blasting machine into particles having equal lengths, and performing boiling drying to obtain the immobilized cell for tagatose production. According to the present invention, separation and purification steps of an enzyme required in tagatose production are simplified, the recycling rate of the enzyme is improved, and the recycling of the enzyme is achieved.

The invention discloses a tagatose 6-phosphate epimerase mutant and application thereof in preparation of tagatose, and belongs to the technical field of enzyme engineering. According to the invention, tagatose 6-phosphate epimerase mutants N195K and V221S with improved conversion rates are obtained through site-directed mutagenesis construction. The mutant N195K promotes the combination of enzyme and Mg < 2 + > while not influencing the combination of Co < 2 + >; the mutant V221S directly changes the metal ion preference of the enzyme, so that the enzyme is more preferred. And the specific activities of the mutants N195K and V221S in the presence of Mg < 2 + > are 2.3 times and 2.8 times of those of the wild type respectively. Under current conditions, the yield of tagatose prepared by the wild type is 22.82%, and the yields of mutants N195K and V221S are respectively 43.64% and 46.62%, which are respectively improved by 91.24% and 104.29% compared with those of the wild type.