135 results about "Tagatose" patented technology

The present application relates to the field of biotechnology, and in particular to an epimerase for synthesizing D-tagatose and its application, wherein the epimerase produces a mutation at position 100, or at position 125, or at position 127, or at position 131, or at position 268, or at position 270, or at position 271, or at position 308, or at position 339, or at position 340, or at position 342, or at position 366, or at position 402, or at position 437, or at position 440 in the amino acid sequence shown in SEQ ID NO. 142; through the above-mentioned method, the epimerase exhibits excellent epimerization activity at the 4th carbon position of D-fructose, can efficiently convert D-fructose into D-tagatose, and is beneficial to improving the synthesis efficiency of D-tagatose.

The invention relates to the technical field of processing technology evaluation, and discloses a tagatose milk tea processing technology evaluation method and system, and the method comprises the steps: carrying out the collection and preprocessing of multi-dimensional parameter data in the production process of tagatose milk tea, and obtaining a multi-dimensional time series data set; a TSVT-PNNT model is input for data reconstruction, and reconstructed data is obtained; performing multi-feature complementary fusion to obtain full-band fusion features; performing process parameter constraint analysis to obtain a process parameter constraint range; generating a plurality of candidate process parameter adjustment schemes by using a Bayesian network and a reinforcement learning method; and in various production scenes, verifying and evaluating the plurality of candidate process parameter adjustment schemes, selecting the optimal candidate process parameter adjustment scheme as a target process parameter adjustment scheme, and outputting the target process parameter adjustment scheme. According to the invention, an optimized process parameter adjustment scheme can be adaptively generated according to different production scenes, so that the production efficiency and the product quality stability of the tagatose milk tea are improved.

The invention relates to a production monitoring management method and system for tagatose sugar-free tea. The method comprises the following steps: carrying out correlation analysis on key parameters in the production process of the tagatose sugar-free tea to obtain a correlation production parameter set containing temperature, stirring speed, pH value, solubility, tagatose addition amount, tea extraction temperature and tea extraction time; performing quality index prediction through the quality index prediction model to obtain a quality index prediction value; predicting by taking the quality index predicted value and the temperature-stirring speed data subset as the input of a two-stage learner-evaluator multi-task sequential neural model, and creating a temperature and stirring speed control strategy; a temperature and stirring speed control strategy is applied to the production process of the tagatose sugar-free tea, a quality related characteristic set of the production process is collected, quality evaluation and fusion analysis are carried out, and a comprehensive quality evaluation result is obtained. The implementation of the invention improves the production efficiency and quality stability of the tagatose sugar-free tea.

The invention belongs to the field of biological pharmacy and biotransformation, and particularly relates to a tagatose-4-epimerase mutant and application thereof. The tagatose-4-epimerase mutant disclosed by the invention is obtained by carrying out single mutation or combined mutation on an amino acid residue at a specific position of an amino acid sequence as shown in SEQ ID NO.1. Compared with wild type tagatose-4-epimerase, the mutant has the advantage that the catalytic activity is improved when the D-tagatose is prepared through conversion. The obtained tagatose-4-epimerase mutant has the advantages of being mild in reaction condition, green, environmentally friendly, single in product and easy to separate and purify, and the production cost is greatly reduced.

The invention discloses L-arabinose isomerase, engineering bacteria and application, the amino acid sequence of the L-arabinose isomerase is shown as SEQ ID NO: 3, and the gene sequence for coding the L-arabinose isomerase is shown as SEQ ID NO: 2. On the basis of a genome of a Peribacillussp.S4 strain, a gene for coding the L-arabinose isomerase is excavated, and a stable prokaryotic expression system is constructed by performing codon optimization on the gene, so that the large-scale controllable production of the L-arabinose isomerase is realized, the yield of the enzyme is remarkably increased, the production cost of the enzyme is reduced, and the method is suitable for industrial production. A stable and economical enzyme source is provided for the production of D-tagatose; the L-arabinose isomerase expressed by the constructed engineering bacteria can significantly improve the yield of D-tagatose, and industrialization of D-tagatose is facilitated.

The invention relates to the technical field of microorganisms and food biology, and provides a lactobacillus paracasei and tagatose composition capable of synergistically treating functional constipation, the active ingredients of the composition are lactobacillus paracasei ZYhyy-004 and tagatose, the lactobacillus paracasei is named as ZYhyy-004, the tagatose is named as ZYhyy-004, the tagatose is named as ZYhyy-004, and the tagatose is named as ZYhyy-004. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the classification name of the strain is Lacticaseibacillus paracasei, the preservation number of the strain is CGMCC No.31554, and the preservation date of the strain is August 5, 2024. Experiments prove that in the composition of the lactobacillus paracasei and the tagatose, the lactobacillus paracasei ZYhyy-004 and the tagatose have a synergistic effect in treating and / or improving the functional constipation of animals, and can be used for improving or treating the functional constipation.

The invention belongs to the field of biological pharmacy and biotransformation, and particularly relates to a tagatose-4-epimerase mutant, a coding gene and application. The tagatose-4-epimerase mutant disclosed by the invention is obtained by carrying out single mutation or combined mutation on an amino acid residue at a specific position of an amino acid sequence as shown in SEQ ID NO.1. Compared with wild type tagatose-4-epimerase, the mutant has the advantage that the catalytic activity is improved when the D-tagatose is prepared through conversion. The obtained tagatose-4-epimerase mutant has the advantages of being mild in reaction condition, green, environmentally friendly, single in product and easy to separate and purify, and the production cost is greatly reduced.

The present invention relates to a biofermentation method of tagatose, and in particular to a tagatase enzyme for catalytic synthesis at high temperature and its application. The enzyme comprises tagatose six phosphate synthase and tagatose six phosphate phosphatase, wherein the amino acid sequence of the tagatose six phosphate synthase is shown in SEQ ID NO.1, and the amino acid sequence of the tagatose six phosphate phosphatase is shown in SEQ ID NO.5. The tagatase enzyme provided by the present invention has higher temperature stability and is more conducive to improving the efficiency of converting starch into tagatose.

The invention belongs to the field of biological pharmacy and biotransformation, and particularly relates to a tagatose-4-epimerase mutant as well as a coding gene and application thereof. The tagatose-4-epimerase mutant disclosed by the invention is obtained by carrying out single mutation or combined mutation on an amino acid residue at a specific position of an amino acid sequence as shown in SEQ ID NO.1. Compared with wild type tagatose-4-epimerase, the mutant has the advantage that the catalytic activity is improved when the D-tagatose is prepared through conversion. The obtained tagatose-4-epimerase mutant has the advantages of being mild in reaction condition, green, environmentally friendly, single in product and easy to separate and purify, and the production cost is greatly reduced.

The invention belongs to the technical field of biology, and particularly relates to recombinant bacillus subtilis, a preparation method of fermentation liquor, immobilized cells, a preparation method of the immobilized cells, a method for synthesizing D-tagatose and application of the D-tagatose. The invention provides a strain of recombinant bacillus subtilis BLCY-012, and the preservation number of the recombinant bacillus subtilis BLCY-012 is CGMCC (China General Microbiological Culture Collection Center) No.31950. The recombinant bacillus subtilis BLCY-012 disclosed by the invention can be fermented to generate the D-tagatose-4-epimerase, the D-tagatose-4-epimerase is an intracellular enzyme, the D-tagatose-4-epimerase can be used for preparing the D-tagatose by taking the D-fructose as a raw material, the immobilized cell is prepared by utilizing the recombinant bacillus subtilis BLCY-012, and the recombinant bacillus subtilis BLCY-012 can be used for preparing the D-tagatose. The immobilized cell is used as an enzyme carrier to convert D-fructose into D-tagatose, the conversion rate can reach 28%-32%, and the conversion rate is high.

The invention belongs to the technical field of biotechnology and biocatalysis, and particularly relates to a method for preparing D-tagatose by catalyzing isomerization of D-fructose. The invention provides a method for preparing D-tagatose by catalyzing isomerization of D-fructose, which comprises the following steps: by taking a wet cell obtained by inducible expression of a recombinant genetically engineered bacterium containing a D-tagatose-4-epimerase gene as a catalyst and D-fructose as a substrate, when the concentration of the substrate is higher than 300g / L, continuously supplementing the substrate and enzyme to prepare the D-tagatose by adopting a strategy of continuously supplementing the substrate and enzyme. The substrate inhibition is reduced, the conversion rate of the D-tagatose is increased, and the final conversion rate of the D-tagatose is increased from 19.5% to 24.2%.

The invention discloses an aldose reductase mutant and application thereof in production of D-tagatose, and belongs to the technical field of genetic engineering. The aldose reductase mutant disclosed by the invention has relatively high catalytic activity, and can be coupled with galactitol 2-dehydrogenase to realize cyclic regeneration of NADH (nicotinamide adenine dinucleotide), so that the preparation cost of D-tagatose is greatly reduced. When the aldose reductase mutant is used for preparing D-tagatose, 98.2 g / L of D-tagatose can be obtained through conversion within 5 h, the yield reaches up to 98.2%, and the conversion effect is better than that of wild type and other mutant enzymes, so that the aldose reductase mutant has good industrial application prospects.

The invention discloses a galactitol dehydrogenase mutant and application of the galactitol dehydrogenase mutant in preparation of D-tagatose, and relates to the technical field of biology. The amino acid sequence of the galactitol dehydrogenase mutant is as shown in SEQ ID NO. 3. According to the invention, a high-throughput screening method based on NTB-PMS detection is used for carrying out semi-rational transformation on a galactitol dehydrogenase mutant derived from Rhizolium leguminosarum, so that the galactitol dehydrogenase mutant with high catalytic activity and thermal stability is obtained. Among all reported galactitol dehydrogenase at present, the mutant has the highest enzyme activity, the half-life period of the mutant reaches 128 h under the conditions that the pH is 8.0 and the temperature is 35 DEG C, and the mutant has important industrial application prospects.

The invention relates to the field of food processing, in particular to a tagatose milk tea processing technology evaluation system. In the background technology, tagatose as a low-calorie sweetening agent has a wide application prospect, but the tagatose faces challenges on solubility, flavor matching and process stability in milk tea processing, and the prior art lacks targeted intelligent evaluation means, resulting in fluctuation of product quality and low production efficiency. In order to solve the problem, the invention provides a system which comprises a multi-parameter sensor module, a data processing and analysis module, a process optimization and feedback control module and a user interaction module. The system adopts a formula S and an anomaly detection formula D to realize real-time monitoring and dynamic optimization. According to the embodiment verification, the product quality consistency is improved by 20%, the production efficiency is improved by 30%, the method is suitable for various formulas, the problems of tagatose crystallization and other abnormalities are solved, the requirements of low-sugar healthy drinks are met, and the method has remarkable innovativeness and practicability.

The invention provides a mutant of xylose reductase and application of the mutant in synthesis of D-tagatose. According to the present invention, the xylose reductase (XR) derived from Saccharomyces cerevisiae is subjected to molecular modification on the basis of the sequence and structure-oriented semi-rational modification strategy so as to obtain the high activity xylose reductase mutant, and the xylose reductase mutant has the following characteristics that: the xylose reductase (XR) derived from Saccharomyces cerevisiae is subjected to molecular modification so as to obtain the xylose reductase mutant, according to the invention, the mutant of the xylose reductase is integrated into a redox pathway for synthesizing the D-tagatose by virtue of a genetic engineering means, and is expressed in bacillus subtilis 168, so that a recombinant engineering bacterium for synthesizing the D-tagatose by fermenting D-galactose is successfully constructed, the efficient synthesis of the D-tagatose is realized, and the yield of the D-tagatose is improved. And the method has obvious practical application value and economic benefit.

The invention relates to the technical field of tagatose preparation, and particularly discloses a method for preparing tagatose and application. The method for preparing tagatose comprises the following steps: (1) mixing a galactose solution and an alkaline calcium isomerization agent, and carrying out an isomerization reaction; (2) carrying out acid neutralization reaction by adopting a mixed acid solution; acid in the mixed acid solution comprises phosphoric acid and citric acid; the mass ratio of the phosphoric acid to the citric acid is 1: (1-6); the pH value of the acid neutralization reaction is 2-5.0. According to the preparation method disclosed by the invention, the environmental pollution caused when phosphoric acid is singly used for production conventionally is reduced, the production safety is improved, and an ideal tagatose yield is obtained.

The invention belongs to the technical field of biology, and particularly relates to a method for synthesizing D-tagatose through multi-enzyme cascade catalysis, lactose is used as a substrate, and the D-tagatose is synthesized through synchronous cascade catalysis of beta-galactosidase, L-arabinose isomerase, glucose isomerase, fructokinase, tagatose-1, 6-diphosphate aldolase, polyphosphate kinase and phosphatase. According to the invention, the intermediate product D-glucose is continuously converted into D-tagatose, so that the conversion rate of D-tagatose is increased, a new method is provided for synthesizing D-tagatose from lactose, and certain theoretical basis and technical support are provided for realizing industrial production of D-tagatose with high added value.

Disclosed herein are formulations (including sweet proteins and rare sugars) for improving the sweetness of low glycemic index rare sugars without altering the texture and flavor of the rare sugars and methods for improving the sweetness of rare sugars. More specifically, the present invention discloses methods for improving the sweetness of rare sugars without altering the sweetness profile, texture and flavor of the primary sugars. The invention also discloses the addition of Brazitame protein with liquid rare sugars at different temperatures during the synthesis of the rare sugars, before crystallization or before or after caramelization of the rare sugars, to increase sweetness. The Brazilian sweet protein increases the sweetness of rare sugars, but is not limited to D-psicose, D-tagatose, D-allose, isomaltulose, trehalose, sorbose, pinose, leucobiose and the like. The invention also provides for the evaluation of non-nutritional sweeteners (such as aspartame, acesulfame, sodium cyclamate, neotame, saccharin, sucralose, stevioside, etc.), as each has a limitation of taste quality if used with rare sugars. However, the use of Brazilian sweet protein does not alter the flavor and texture of rare sugars, and the taste profile matches sucrose.

The present invention relates to a use of an enzyme belonging to the aldolase group and comprising a polypeptide having at least 30% identity with an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 4, for stereoselectively preparing D-tagatose-1,6-bisphosphate (TBP) or D-tagatose-1-phosphate. The present invention also relates to a method for preparing D-tagatose-1,6-bisphosphate (TBP) or D-tagatose-1-phosphate, comprising a step of reacting, in a suitable reaction medium, dihydroxyacetone phosphate (DHAP) or at least an isomer thereof or analog thereof, and optionally D-glyceraldehyde, with an enzyme belonging to the aldolase group, said enzyme comprising a polypeptide having at least 30% identity with an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 4. Finally, the present invention relates to a process for preparing D-tagatose.

The invention discloses a C4 epimerase whole cell breeding method and a D-tagatose preparation method. C4 epimerase whole cells obtained through breeding grow robustly, are excellent in performance and are more suitable for recombinant escherichia coli for production; when the whole cell is used for producing D-tagatose, a whole cell culture does not need to be crushed, and other raw materials, catalysts and the like do not need to be added; the method has the advantages of simple reaction conditions, high efficiency, single reaction, few side reactions, easiness in control and industrial production.

The invention belongs to the technical field of food coloring, and particularly relates to a compound sweetening agent capable of coloring at low temperature for food as well as a preparation method and application of the compound sweetening agent. The D-psicose and tagatose chewable tablets are prepared from the following raw materials in parts by mass: 15 to 20 parts of D-psicose, 1 to 5 parts of tagatose, 2 to 8 parts of D-xylose, 20 to 25 parts of xylitol, 30 to 35 parts of solid maltitol, 30 to 35 parts of liquid maltitol, 10 to 14 parts of polydextrose and 0.5 to 1.5 parts of stevioside. The sweetening agent with a coloring function is perfectly combined with other sugar-free sweetening agents, so that function synergy is realized, food can quickly generate good color and luster in a low-temperature state, and the nutrition of the food is prevented from being lost.

The present application relates to a fructose-4-epimerase variant exhibiting tagatose conversion activity and a method for preparing tagatose using the same.

To provide an immobilized enzyme composition for hexose production or an improved process for hexose production. [Solution] The present invention relates to an immobilized enzyme composition for the preparation of hexoses. Examples of hexoses include tagatose, psicose, fructose, allose, mannose, galactose, altrose, talose, sorbose, gross, idose, and inositol. The present invention also relates to an enzymatic process for preparing hexoses from sugars by contacting starch derivatives with the immobilized enzyme composition of the present invention.

The invention relates to a biological fermentation method of tagatose, in particular to application of a product in catalyzing dephosphorization of tagatose hexaphosphate to form tagatose. The product is tagatose hexaphosphate phosphatase, nucleotide for coding the tagatose hexaphosphate phosphatase or a microorganism for expressing the tagatose hexaphosphate phosphatase, and the amino acid sequence of the tagatose hexaphosphate phosphatase is as shown in SEQ ID NO.5. The tagatose provided by the invention has higher temperature stability, can highly selectively dephosphorize tagatose hexaphosphate under a higher temperature condition, and greatly reduces the generation of byproducts such as glucose, fructose and the like.

The invention relates to the technical field of biology, in particular to epimerase for synthesizing D-tagatose and application. Epimerase producing mutation of 100th or 125th or 127th or 131 or 268th or 270th or 271th or 308th or 339th or 340th or 342th or 366th or 402th or 437th in amino acid sequence as shown in SEQ ID NO.7 mutation at the 440th site; through the mode, the epimerase shows excellent epimerization activity at the fourth carbon position of D-fructose, can efficiently convert D-fructose into D-tagatose, and is beneficial to improving the synthesis efficiency of D-tagatose.

The utility model discloses an ion exchange device for tagatose production, which relates to the technical field of ion exchange and comprises a base, a fixing support is fixedly connected to the upper end of the base, and four fixing rods are fixedly connected to the side wall of the fixing support. The end, away from the fixing support, of the fixing rod is fixedly connected with a first exchange assembly, a second exchange assembly, a third exchange assembly and a fourth exchange assembly respectively, and the first exchange assembly, the second exchange assembly, the third exchange assembly and the fourth exchange assembly each comprise an installation cylinder. The position of the inner cylinder in the mounting cylinder can be conveniently adjusted, so that the height of the whole ion exchange assembly can be flexibly adjusted, the device can meet the mounting requirements of equipment with different heights, the applicability and the flexibility of the device are greatly enhanced, and in the mounting or dismounting process, only the limiting cylinder needs to be simply rotated, and the mounting and dismounting are convenient. Therefore, the pipeline can be quickly spliced with or detached from the liquid outlet at the lower end of the mounting cylinder and the liquid inlet at the upper end of the inner cylinder.

The utility model provides an external circulation vacuum concentration mechanism for tagatose production. The external circulation vacuum concentration mechanism comprises a heating assembly, a main body assembly and a bottom plate, the heating assembly comprises a heating tank, a rotating disc, a U-shaped frame, a stirring motor, a rod body, a first stirring rod, a cleaning scraper blade, a second stirring rod, a water pump, a water tank, a first water pipe, a second water pipe, a rotating nozzle, a blow-off pipe and a feeding hole; the bottom of the heating tank is fixedly connected to the top of the bottom plate, and the outer side wall of the rotating disc is rotatably connected to the interior of the heating tank. The stirring motor is controlled to drive the first stirring rod to rotate, so that the cleaning scraper on the first stirring rod cleans the inner wall of the heating tank; and then a water pump is controlled to convey water in a water tank into a second water pipe, so that a rotary spray head flushes the inner wall of the heating tank, a stirring rod and a cleaning scraping plate, the sanitation in the heating tank is ensured, and the situation that residues are accumulated in the heating tank and follow-up use is affected is avoided.

The present application relates to the technical field of biotechnology, and particularly relates to a tagatose-6-phosphate phosphatase mutant and a method for synthesizing tagatose from scratch. The method provided by the present application is a one-pot method for biosynthesizing D-tagatose by using starch or dextrin as a substrate, adopting a multi-enzyme cascade reaction system composed of a tagatose-6-phosphate phosphatase mutant, a glycogen-debranching enzyme, an alpha-glucan phosphorylase, a phosphoglucomutase, a glucose-6-phosphate isomerase, a D-tagatose-6-phosphate-3-epimerase and a 4-alpha-glucan transferase. The method can prepare tagatose from ordinary and inexpensive starting materials (starch or dextrin) at a high yield, improves the overall conversion rate of the system and the final yield of tagatose, provides a new technical approach for realizing low-cost and green production of tagatose, and has the advantages of high yield, low cost and being more suitable for industrial production.

The application belongs to the technical field of biology and specifically relates to a method for synthesizing D-tagatose through multi-enzyme cascade catalysis, which uses lactose as a substrate and utilizes beta-galactoside enzyme, L-arabinose isomerase, glucose isomerase, fructokinase, tagatose-1,6-diphosphate aldolase, polyphosphate kinase and phosphatase for synchronous cascade catalysis to synthesize D-tagatose. The method improves the conversion rate of D-tagatose by continuously converting the intermediate product D-glucose into D-tagatose, provides a new method for synthesizing D-tagatose from lactose, and provides certain theoretical basis and technical support for realizing the industrialized production of high-value-added D-tagatose.

The invention discloses a high-induction-activity promoter derived from a tagatose operon of bacillus licheniformis, and belongs to the field of gene engineering. The promoter PfruK2 capable of being induced by a carbon source in a PTS (Pleurotropin Tyrosine Sequence) way is screened, the induced carbon substrate spectrum capable of being responded by the promoter is wide, the promoter is not affected by a glucose-mediated CCR effect, the induction intensity is high, and the induction peak value is improved by more than 50% compared with that of a quorum sensing strong promoter PLan.