The invention belongs to the field of
biology and
chemical industry, and particularly discloses a method for continuously producing D-
tagatose by adopting D-
galactose as a
raw material on the basis of enzymatic
isomerization reaction and a continuous
chromatographic separation in-situ
coupling technology. The method comprises the following steps of preparing a buffering solution; adequately dissolving the thermophilic L-
arabinose isomerase in the water to obtain an
enzyme solution; adequately dissolving D-
galactose in the water to obtain a
raw material solution; mixing and preheating the
raw material solution, the buffering solution and the
enzyme solution to obtain a feeding solution of a
simulated moving bed reactor (SMBR); and placing the feeding solution into the SMBR to obtain a D-
tagatose product solution through the in-situ reaction and separation. By determining the appropriate operating condition of the SMBR, the conversion rate of the D-
galactose reaches more than 80 percent, the purity of the D-
tagatose reaches more than 95 percent, and the yield is more than 80 percent. The method is low in cost, less in
pollution and high in conversion rate; moreover, continuity in production can be realized, the consistency of the product quality can be guaranteed, and the commercialized application prospect is good.