148 results about "Resting Cell" patented technology

The invention discloses a whitening mask which is prepared by dipping a liquid prepared from the following raw materials in parts by weight: 0.1-3 parts of micromolecular hyaluronic acid, 0.1-2.5 parts of macromolecular hyaluronic acid, 0.7-30 parts of synthetic egg white powder, 1-7 parts of an aloe extract, 0.7-9 parts of propylene glycol, 5-50 parts of 1,2-butanediol, 5-30 parts of glycerinum, 40-80 parts of a ginseng extract liquid, 0.1-1 part of glabridin, 0.3-2 parts of alpha-arbutin, 0.1-1 part of phloretin, 0.1-1 part of apple stem cells, 1-5 parts of raspberry ketone glucoside, 0.1-1 part of tetrahydropiperine, 1-10 parts of vitamin c ethyl ether, 1-5 parts of tranexamic acid, 1-10 parts of hydrolyzed pearl and added to 1000 parts of water on mask paper. The whitening mask has the efficacies of promoting skin absorption of various effective components, repairing damaged cells, activating resting cells, promoting cell regeneration, moisturizing the skin, rapidly and efficiently whitening the skin and removing freckles, and is free of irritation, allergy or damage to the skin.

The invention discloses a novel CTC (circulating tumor cell) enrichment technology and a preparation method of a kit. The technology comprises the steps as follows: through permutation and combination of all possible CD45 isomer protein sequence information, designing antigens, thereby producing multiple antibodies, screening out one antibody capable of recognizing all CD45 isomers, coupling the antibody onto a magnetic bead, and further capturing all leukocytes from blood; collecting 10 ml of peripheral venous blood; removing red blood cells through lysis by an osmosis lysis buffer; mixing a designed specific antibody, the magnetic bead and the rest cell sap containing no red blood cells to separate leukocytes; and obtaining a cell population containing CTCs with higher concentration. According to the technology, a negative collection technology is adopted, so that the proportion of the CTCs is increased substantially, and the rapid and accurate detection is facilitated; meanwhile, a negative screening means is adopted, so that the CTCs are reserved without loss, and the fact that tumor diseases cannot be detected and the treatment for patients is delayed due to the loss of certain CTCs is prevented.

The invention discloses a Rhodococcus (Rhodococcus sp.ECU0066) and the use thereof, and the culture collection number of the strain is CGMCC No.2547. The resting cells of the strain is taken as a biological catalyst, and prochiral phenyl alkyl sulfide and a derivative thereof are carried out catalytic oxidation asymmetrically to obtain benzoylate sulfoxide and a derivative thereof with optical activity. The strain and the stereoselective biological oxidation process have the advantages of better catalysis effect, simple and safe operation and low cost, the product is easily purified and is friendly to environment.

The invention provides antibodies that specifically bind a membrane protease complex, the complex consisting of two homodimers of seprase and dipeptidyl peptidase IV (DPPIV), obtained from mammalian, preferably human cell membranes. The antibodies specifically bind the DPPIV protease of the seprase-DPPIV complex. This membrane protease complex resides on cell surface invadopodia at the leading edge of angiogenic endothelia, migratory fibroblasts, and invading cancer cells. The antibodies and immunoconjugates of the invention specifically bind the membrane protease complex at the cell surface invadopodia, yet fail to react with resting cells in adjacent human tissues and blood vessels. These antibodies and immunoconjugates block interaction of collagen matrix with the seprase-DPPIV complex in the invasive cells during angiogenesis and cancer spreading but not that with other endothelia or tumor cells. The invention further provides methods for identifying and of using DPPIV antagonists to inhibit capillary sprouting, angiogenesis and cancer invasion in tumor tissues and metastases. Also provided are therapeutic compositions comprising DPPIV antagonists.

The invention discloses an engineering bacteria and a method for preparing tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate. The engineered bacteria comprises an alcohol dehydrogenase gene, a glucose dehydrogenase gene and a carbonyl reductase gene; the method comprises the following steps of culturing engineering bacteria and inducing the expression of enzymes, collecting cells by virtue of centrifuging, resuspending with a buffer to obtain a resting cell suspension; adding 6-tert-butyl-chloro-3,5-carbonylhexanoate, glucose and isopropyl alcohol into the resting cell suspension, reacting, after the reaction is completed, separating and purifying the reaction liquid to obtain the tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate. By virtue of the method, one-step production of the tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate from 6-tert-butyl-chloro-3,5-carbonylhexanoate is realized, the process of the tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate is simplified, and the production cost is reduced since the addition of the additional coenzyme is avoided.

The invention discloses a method for preparing levodopa by virtue of an enzymic method. The method comprises the following steps: picking up an annular stenotrophomonas maltophilia strain slant for inoculation into a seed culture medium for culturing, thereby obtaining a primary seed liquid; inoculating a fermentation culture medium with the primary seed liquid at the inoculum size of 3-10% for fermentation culturing, centrifuging after the fermentation is ended and collecting bacterial cells; and adding tyrosine and the bacterial cells to a buffer solution to have an enzymatic reaction under the conditions of 18-30 DEG C and the pH of 5.0-6.0, thereby converting the tyrosine into the levodopa. According to the method, due to the resting cell transformation technology, and the preferred intermittent weak ventilation technology, adopted in the transformation process, the catalytic efficiency of the enzyme is improved; the concentration of the levodopa is 27g / L and the molar transformation rate of the tyrosine is above 99%; and besides, the method is mild in conditions, high in enantio-selectivity and free from racemization effect, is more suitable for industrial production, and has remarkable economic benefit and industrial application value.

The application provides a method and a system for determining the downward inclination angles of antennas in order to resolve a problem of ineffectively-controlled cell interference and coverage caused by unreasonably-arranged downward inclination angles of the antennas. The method comprises: obtaining the normal direction and the positional information of antennas in all cells in a preset area; successively determining cells subjected to interference in the rest cells by using the ith cell as an interference source cell and according to the normal direction and the positional information of the antenna in the ith cell and the positional information of rest cells except the ith cell; respectively calculating the cell distance between the ith cell and each cell subjected to interference; calculating the coverage distance of the ith cell according to the cell distance; calculating an initial antenna downward inclination angle of the ith cell according to the coverage distanct; and comparing the initial antenna downward inclination angle with a preset antenna downward inclination angle in order to determine the actual antenna downward inclination angle of the ith cell. The antenna downward inclination angle determined by the method is reasonable so as to guarantee the coverage of the own cell and inhibit adjacent cell interference.

A microbial ethyl esther sophorolipid derivative with no acetylated groups produced by Candida species, for treating and preventing sepsis / septic shock. The method of producing sophorolipids is through microbial resting cells of Candida bombicola. The sophorolipids obtained from resting state cultures are isolated as a complex mixture of compounds and then decanted as a dense oil from the culture broth, subsequently washed to remove free fatty acids. Secondary chemical transformation via base catalyzed hydrolysis is used to reduce the 8 possible structural sophorolipid species to a single moiety, the 17-L-[(2′-O-b-D-glucopyranosyl-b-D-glucopyranosyl)-oxy]-cis-9-octadecenoate de-acetylated free acid. The compound acts primarily through decreasing inflammatory cytokines and eliciting other synergistic anti-inflammatory mechanisms by blocking TLR4-CD14 upstream of the inflammatory signaling cascade. The compound can be administered either intraperitoneally or intravenously at single or multiple doses of 5-30 mg / kg of weight in solvent media or in capped nanoparticles, preferably within 48 hours of sepsis inception.

The invention discloses a method for bioconverting conjugated linoleic acid by using Lactobacillus plantarum, which comprises the following steps: inoculating Lactobacillus plantarum CGMCC NO.3785 to an MRS (Man, Rogosa and Sharpe) culture medium containing linoleic acid, preculturing, and carrying out refrigerated centrifugation to obtain resting cells; and inoculating the resting cells to a citric acid-sodium citrate buffer solution containing 3.0-25 mg / ml of linoleic acid, and carrying out shake culture for 84-120 hours. The method for converting LA (linoleic acid) into CLA (conjugated linoleic acid) does not need a high-temperature high-pressure environment, and the product can be separated conveniently; and the resting cells obtained by centrifugation are used for catalyzing the synthesis of CLA, thereby preventing the overhigh-concentration LA from inhibiting the growth of bacteria and further achieving the goal of high CLA yield.

The invention discloses a method for preparing high-purity danshinolic acid B, which comprises the following steps: (1) mixing a red sage root aqueous extract with Fusarium graminearum resting cells,and performing biotransformation at a temperature of between 20 and 35 DEG C for 24 to 90 hours to obtain a red sage root aqueous extract without rosmarinic acid; and (2) adsorbing the red sage root aqueous extract without the rosmarinic acid by anti-phase resin, and eluting the red sage root aqueous extract without the rosmarinic acid to obtain the high-purity danshinolic acid B. The purity of the danshinolic acid B prepared by the method is more than or equal to 99 percent, the recovery percent is over60 percent, the process is simple, and the cost is low.

The invention belongs to the technical field of biological catalysis, and relates to microbacterium oxydans and a method for preparing optically pure (R)-1-[3,5-bis(trifluoromethyl) phenyl] ethanol by using the microbacterium oxydans. The preservation number of the microbacterium oxydans C3 is CCTCC M2010179. The microbacterium oxydans is cultured at the temperature of 27-33 DEG C for 24-48 hours, and then substrate 3,5-bis-trifluoromethyl acetophenone is added to convert auxocyte; or the culture bacterium is collected and is resuspended in a buffer solution, and then the substrate is added to convert resting cells so as to generate a target product, namely the optically pure (R)-1-[3,5-bis(trifluoromethyl) phenyl] ethanol. The microbacterium oxydans has the advantages of easiness in industrialization, easiness in preparation of a catalyst thereof, mild reaction conditions and high conversion efficiency.

The present invention relates to Bacillus subtilis Fds-1 with the preservation number of CGMCC No. 0644. The strain of the present invention is obtained through separation from the soil near high sulfur oil well, ultraviolet mutagensis and screening. The growing cell and the rest cell of the strain can make the C-S bond oxidized to break and thus eliminate organic sulfur from organic compound, so that the strain is especially suitable for eliminate organic sulfur from petroleum fractions in high desulfurizing rate.

The invention provides a strain Arthrobacter sp.ZJUTW with high phthalate degrading property and an application of the strain. The strain is conserved in the China Center for Type Culture Collection (address: Wuhan University, Wuhan City, China 430072), the preservation number (CGMCC No) is 2012246 and the conservation date is June 24, 2012. The new strain with high phthalate degrading property is provided, the diethyl phthalate (DEP) and dibutyl phthalate (DBP) degrading conditions of resting cells are optimized and the environmental pollution problem of phthalate compounds can be effectively solved.

The invention discloses a making method of chicken embryo extracts. The making method includes the following steps that chicken embryonic stem cells are subjected to cell membrane rupturing treatment and impurity removing and extracted, and chicken pluripotent embryo extracts are obtained. The invention also discloses chicken embryo extracts obtained with the making method and an application thereof. The chicken pluripotent embryo extracts are separated from the chicken embryonic stem cells, the effect of human embryo extracts is achieved, various cells in the human body can keep the activity, and service life of working cells is prolonged; injured cells are repaired, and the cell functions are recovered; in-vivo resting cells are activated, effective working cells are supplemented; reduction of stem cells is protected, and generation of the stem cells is promoted; resting stem cells are activated to differentiate new cells for replacing dead cells, and the viscera recovering effect is achieved.

The invention relates to a method for preparing 10-hydroxy-2-caproleic acid through escherichia coli engineering bacteria resting cells. The method includes the following steps that (1) escherichia coli engineering bacteria containing recombinant plasmids pET-28a-ydiI is constructed; (2) the escherichia coli engineering bacteria containing the recombinant plasmids pET-28a-ydiI is taken to prepareinduced cells; and (3) the induced cells are cultured in a transformed culture medium to obtain the resting cells, and 10-hydroxy capric acid is then added into the culture medium to prepare the 10-hydroxy-2-caproleic acid. According to the method for preparing the 10-hydroxy-2-caproleic acid through the escherichia coli engineering bacteria resting cells, positive recombinant escherichia coli, containing thioesterase, modified through genetic engineering is disclosed for the first time, after special induction treatment, the 10-hydroxy-2-caproleic acid can be produced by fermentation throughthe resting cells, and a large quantity of biosynthesis of the 10-hydroxy-2-caproleic acid is realized.

The invention provides a method for preparing a steroid drug intermediate employing bioconversion phytosterol. The method comprises the following steps: 1) providing turbid liquid of phytosterol; 2) providing a resting cell of a microbial strain for converting the phytosterol into a steroid drug intermediate; 3) mixing the turbid liquid of the phytosterol with the resting cell, and adding beta-cyclodextrin or chemically modified beta-cyclodextrin to evenly mix, and cultivating to finish conversion; 4) centrifuging the conversion liquid obtained in the step 3), separating supernatant from thallus, separating to obtain the steroid drug intermediate from the supernatant by solvent extraction. By adopting the method provided by the invention, the sterol feeding concentration is improved, the target product quantity is increased, simple separation of the thallus, a cosolvent and a product is achieved when the conversion lasting time is shortened, repeated use of the thallus and the cosolvent for a plurality of times is achieved, the production cost is greatly saved, and the method is applicable to industrial production.

The present invention discloses one kind of Bacillus alcaligenes and the process of preparing single antimer mandelic acid therewith. The resting cell of the Bacillus alcaligenes, ECU0401 CGMCC No.2009, is used as the biological catalyst in selectively converting racemized mandelic acid and its derivative to prepare optically active mandelic acid and its derivative. The Bacillus alcaligenes strain of the present invention has high catalyzing effect, and the product (R)-(-)-mandelic acid has optical purity over 99.9 %, obviously higher than that obtained with catalyzing yellow Bacillus brevis AS1.818 and with catalyzing Saccharomyces cervisiae AS2.150.

The invention relates to a method for preparing 10-hydroxyl-2-decenoic acid by utilizing escherichia coli engineering bacteria. The method comprises the following steps that (1) a recombinant plasmidpBbB5K-P450 fusion enzyme, recombinant plasmids pBbB5K-FadK, recombinant plasmids pBbB5K-MCAD and recombinant plasmids pBbB5K-YdiI are constructed; (2) pBbB5K-ydiI-MCAD-FadK-P450 fusion enzyme recombinant plasmids are constructed; (3) the fusion enzyme recombinant plasmids are converted into escherichia coli, the escherichia coli are screened and subjected to induction culture, and thus inductioncells are prepared; and (4) the induction cells are cultured by a transformation culture medium, thus resting cells are prepared, then decylic acid is added into the culture medium, culturing is conducted, and thus the 10-hydroxyl-2-decenoic acid is prepared. The 10-hydroxyl-2-decenoic acid is produced in a fermented mode by utilizing the decylic acid as a raw material, and the process of producing the 10-hydroxyl-2-decenoic acid with the high added-value through the low-value decylic acid is realized.

The invention discloses stenotrophomonas maltophilia GYH and application thereof in degradation of chlorinated hydrocarbon pollutants. The application comprises the following steps: adding resting cells obtained by expanding culture of stenotrophomonas maltophilia GYH into an inorganic culture medium with a pH value of 5-8, then adding chlorinated hydrocarbon pollutants, and conducting culturing under the conditions of a temperature of 20-35 DEG C and a rotation speed of 140-180 rpm to realize degradation of the pollutants. The stenotrophomonas maltophilia GYH disclosed by the invention can beused for removing chlorinated hydrocarbon pollutants with concentrations in a range of 2.9 mg / L to 8.93 mg / L. Therefore, the stenotrophomonas maltophilia has efficient degradation capability on industrial similar pollutants, and can bear pollutants with a relatively high concentration.

The invention discloses a high-yielding CLA (conjugated linoleic acid) lactobacillus plantarum strain, which is named as 'Lactobacillus plantarum' lp15-2-1 and collected in China General Microbiological Culture Collection Center (CGMCC) (collection date: April 27th, 2010; collection NO.: CGMCC NO. 3782). The strain of the invention can convert linoleic acid into conjugated linoleic acid, wherein the maximum yield of the conjugated linoleic acid in resting cells is 1,023.55 mu g / ml, and the conversion rate of the linoleic acid by direct shaking culture reaches 21.32 percent; therefore, the strain of the invention can be applied to the industrial production of acidophilous milk, pickles, fruit drinks and the like.

The invention discloses streptomyces canus; through identification, it is streptomyces canus, and the bacterial strain name is gl2-18; the streptomyces canus is preserved at China General Microbiological Culture Collection Center; the preservation number is CGMCC No. 13662; the preservation date is February 15, 2017. Compared with the prior art, the resting cell or the growth cell of the new strain streptomyces canus CGMCC 13662 can effectively degrade chloronicotinyl insecticide, in particular to acetamiprid and thiacloprid; the streptomyces canus is used as the pesticide degradation fungicide, and has very good application prospect.

Methods of using microarrays to simplify analysis and characterization of genes and their function are provided. Such methods can be used to identify and characterize antibodies having binding affinity for a specific target antigen. A method of determining gene expression at the protein level by contacting an array of characterized or uncharacterized antibodies on a solid surface with one or more proteins and identifying the antibodies to which said protein(s) binds also is provided. This method can be used to compare the protein expression in two different populations of cells, such as normal cells and cancer cells or resting cells and stimulated cells. In addition, a method of determining gene expression at the protein level by contacting a microarray of nucleic acid samples derived from a variety of different sources with one or more nucleic acid probes then identifying the sample or samples to which the probe binds is provided.

The invention discloses a pseudoxanthomonas indica CGMCC6648 for degrading chloronicotinyl insecticide imidacloprid; the main route for reducing imidacloprid is to carry out hydroxylation on imidacloprid so as to generate 5-hydroxy imidacloprid. Sugar and organic acid are added in a resting cell transformation solution, thus the imidacloprid hydroxylation ability of the pseudoxanthomonas indica CGMCC6648 can be greatly improved.

The invention provides an escherichia coli engineered strain for producing allitol by virtue of biotransformation as well as a construction method and application of the strain, and discloses an escherichia coli recombinant strain II. Experiment shows that D-fructose can be transformed into allitol by virtue of resting cell reaction of the strain, and the rare allitol is wide in application prospect in food and medicine industries.

The invention discloses a processing method for increasing lactobionic acid yield of pseudomonas fluorescence by applying a physical field enhancement policy, which belongs to the technical field of food processing. In the method, after being undergoing resting cell culture, the pseudomonas fluorescence synthesizes lactobionic acid in presence of a catalyst under stress caused by a high-pressure physical field. The processing method comprises the following steps: placing resting cells into a high-pressure reaction kettle according to requirements of biological processing; treating the restingcells under set temperature and pressure conditions for a certain period of time; and after the treatment is finished, regulating the pressure in the reaction kettle to normal temperature. In the invention, the lactobionic acid yield is increased by changing the permeability and structure of cell membrane with a high-pressure physical field technique; and compared with the conventional method, the method has a more obvious substrate conversion rate, adopts a simple processing process, realizes low production cost and meets requirements for industrial production.

The Rhodococcus erythropolis related by the invention is Rhodococcus erythropolis SDUZAWQ, which is stored in 'China microorganism strain preservation management committee common micro organism center' at March 1st, 2004, and whose storage number is CGMCC NO. 1922. The strain is obtained by separating from sewage water from oil-extraction plant or soil. The cell culture fluid of the strain can high effectively remove sulfureous in dibenzothiophene, 4, 6-dimethyl-dibenzothiophene, 4-methyl-dibenzothiophene, benzothiophene, 5-methyl-benzothiophene by oxidative scission of C-S bond. Mixed sulfureous source of dibenzothiophene and sodium sulfate is used for culturing the Rhodococcus erythropolis SDUZAWQ, and the obtained resting cell culture fluid can effectively remove the sulfureous contained in diesel oil which has been subject to hydrofining.

The invention relates to a method for producing polymalic acid through continuous fermentation which can be made into resting cells. The implementation steps are as follows: (1) preparation of slant strains; (2) preparation of seed liquid; (3) the above seed liquid is used for inoculation into Fermentation in a 5L aseptic fermenter; (4) Do not control the pH in the early stage of fermentation, and then continuously add NaOH to control the pH of the fermentation broth to 5.0-5.5 after the emergence of mycelium balls; (5) After 55-60 hours of fermentation, the When the glucose concentration is 8-10g / L, stop the fermentation, add fresh medium again, and repeat the fermentation; (6) Resting cell preparation; (7) Use the same fermentation method for resting cell fermentation; (8) Fermentation The solution was filtered with a hollow fiber membrane with a molecular weight cut-off of 6000Da, added an equal volume of absolute ethanol to precipitate, dried and weighed; the yield of polymalic acid was 50-57g / L. The method can effectively reduce the non-optimal economic stage in the fermentation process, and achieve the purposes of shortening the fermentation time, improving the utilization rate of equipment, and reducing the production cost of products.

The invention discloses a method for preparing chiral (S)-acetoin by virtue of whole-cell biological catalysis. The method comprises the following steps: firstly carrying out coexpression on butanedione reductase genes and formate dehydrogenase genes in escherichia coli, so as to obtain recombinant escherichia coli; and carrying out asymmetric reduction reaction in a water phase by taking a resting cell of the recombinant escherichia coli as a whole-cell biocatalyst, butanedione as a substrate and formic acid or formate as a coenzyme NADH regenerated hydrogen donor, so as to generate chiral (S)-acetoin. A whole coenzyme regeneration system of the cell is utilized, and only a proper amount of formic acid or formate needs to be added as the hydrogen donor, so that the transfer ability of a thallus is improved; expensive coenzyme NADH does not need to be added, and the yield of chiral (S)-acetoin can reach up to 46.1g / L; the process is simple and efficient, the production cost is low, and the problems of low efficiency and high cost of traditional conversion are solved; and the method has an extremely high economic value and is applicable to large-scale business application.

The invention relates to a supplementation and enzymatic conversion based ultrasonic-assisted preparation method of L-tyrosine. The supplementation and enzymatic conversion based ultrasonic-assisted preparation method of L-tyrosine comprises the following steps of: culturing a strain with tyrosine phenol lyase activity by using an slant activated inoculum, inoculating the cultured strain against a fermentation culture medium, carrying out fermentation culturing, and collecting resting cells; adding the resting cells into the buffer solution of potassium phosphate, and putting the buffer solution of potassium phosphate into an ultrasonic device; adding phenol, sodium pyruvate, ammonium chloride and pyridoxal phosphate into the resting cells, converting to synthesize, and filtering to obtain resting cells and a product of conversion, and filtering and drying the product of conversion to obtain L-tyrosine, wherein the resting cells are recycled for next reaction. The supplementation and enzymatic conversion based ultrasonic-assisted preparation method of L-tyrosine comprises has the advantages of simple process, advanced technology, high safety and short production cycle, is suitable for industrial production and has good market prospect.