4 results about "Resting Cell" patented technology

The invention relates to the technical field of biological pharmacy, and discloses a mutant strain for efficient biotransformation synthesis of miglitol, a screening method and application. A compound mutation strategy of ARTP mutagenesis and NTG mutagenesis is utilized, a rapid screening method based on carbonyl color development is combined, a mutant strain capable of efficiently converting and synthesizing a miglittol key intermediate is obtained, the mutant strain is named as Gluconobacter oxydans G1036, and the enzyme activity of the mutant strain is 61.7 U / mL and is improved by about 48% compared with that of an original strain. A process for producing 6NSL by catalyzing NEHG through resting cells of the mutant strain is researched, 73.53 g / L of 6NSL is produced by catalyzing 80 g / L of a substrate within 48 h, and compared with 80.37% of an original strain, the 6NSL is improved by 19.63%. According to the present invention, the research on the process for repeatedly and catalytically producing the 6NSL by using the resting cells of the mutant strain in multiple batches shows the good catalytic performance, and 3 batches can be efficiently utilized to accumulatively produce 220.59 g / L of the 6NSL within 144 h;

The invention discloses fermented vegetable oil and a production method thereof. The invention belongs to a whole-cell enzymatic hydrolysis fermentation process of resting cells, which comprises the following steps: firstly, carrying out high-density fermentation to obtain a saccharomycetes liquid, then separating and washing to obtain clean high-activity saccharomycetes, and mixing the high-activity saccharomycetes, water and vegetable oil in proportion; vegetable oil is fermented by using resting yeast thalli through a whole-cell enzymolysis fermentation process under the conditions of specific temperature, oxygen supply and pH, and after fermentation is finished, secondary centrifugal filtration is performed to obtain the fermented vegetable oil. And compared with the fermented vegetable oil obtained by the conventional microbial fermentation process. The obtained fermented oil has the advantages of fewer impurities, lighter color, cleaner oil, low ash content, stronger oxidation stability, longer shelf life and the like, is rich in free fatty acids, flavonoids, polysaccharides and natural glycolipids compared with natural vegetable oil crude oil, has better skin moisturizing, repairing, soothing and anti-oxidation effects and the like, and is suitable for being used as a skin care product. Good economic values and application prospects are realized in the fields of beauty, skin care and washing.

This invention discloses a Valenciane oxidase mutant and its application in the preparation of naringin. The invention constructs a gene library of the HPO mutant using site-directed mutagenesis. In vitro catalytic experiments using resting cells show that, under the same conditions, the optimal HPO mutant (HPO_G302G-V480A-V482A-A484A, whose amino acid sequence is shown in SEQ ID No. 4 and nucleotide sequence in SEQ ID No. 5) exhibits a catalytic efficiency 2.54 times that of the wild type, thereby increasing substrate utilization efficiency and improving the yield of the target compound, naringin. Furthermore, the bio-preparation method for naringin described in this invention is simple to operate, environmentally friendly, and uses mild reaction conditions, showing great application potential. In addition, this invention is the first report of successfully constructing a beneficial HPO mutant for high-yield naringin production.

Disclosed herein are in vitro methods for detecting the presence of metabolically altered cells. Also disclosed herein are in vitro methods for detecting the presence of stationary cells. Disclosed herein are in vitro methods for detecting and predicting the presence of cancer. Disclosed herein are in vitro methods for monitoring response to anti-cancer therapy. The present disclosure analyzes the expression of at least one biomarker of pluripotent stem cells for detecting or predicting or monitoring cancer. Disclosed herein are related uses of at least one biomarker of pluripotent stem cell markers and methods of treatment including in vitro detection or prediction methods.