59 results about "Rare sugar" patented technology

Beverages comprising rare sugars and sweetness enhancers are provided herein, wherein the sweetness enhancers are present at or below the sweetness recognition threshold concentration. Also provided are methods for improving the sweetness of a beverage comprising rare sugars by adding a sweetness enhancer in a concentration at or below its sweetness recognition threshold. Beverages comprising natural high potency sweeteners and rare sugars with sugar-like characteristics are also provided, wherein the natural high potency sweetener and rare sugars are present in particular weight ratios.

An object of the present invention is to improve a distinctive strange taste (particularly bitterness remaining in the mouth) of a conventional low-calorie food or drink, particularly an edible product (a food or drink) having low calories obtained by replacing part or most of the sugar or isomerized sugar to be added by a high-intensity sweetener. Provided are a composition for improving a taste of a high-intensity sweetener, a method of improving a taste of an edible product containing a high-intensity sweetener, and an edible product in which a taste of a high-intensity sweetener is improved, each of which is characterized by containing or using an isomerized sugar and a rare sugar such that the rare sugar is contained or used in an amount of from 1 to 150 parts by mass based on 100 parts by mass of the isomerized sugar.

Beverages comprising rare sugars and sweetness enhancers are provided herein, wherein the sweetness enhancers are present at or below the sweetness recognition threshold concentration. Also provided are methods for improving the sweetness of a beverage comprising rare sugars by adding a sweetness enhancer in a concentration at or below its sweetness recognition threshold. Beverages comprising natural high potency sweeteners and rare sugars with sugar-like characteristics are also provided, wherein the natural high potency sweetener and rare sugars are present in particular weight ratios.

Zero- and reduced-calorie frozen carbonated beverages comprising rare sugars are provided herein. The beverages contain a freezing point depressant composition comprising at least one rare sugar and, optionally, one or more other freezing point depressant compounds. Frozen carbonated beverages comprising natural high potency sweeteners and rare sugars with sugar-like characteristics are also provided, wherein the natural high potency sweetener and rare sugars are present in particular weight ratios. Methods of preparing the frozen carbonated beverages are also provided.

The invention discloses application of L-rhamnulose-1-phosphate aldolase in catalytic synthesis of rare sugars. In order to avoid the direct use of DHAP (dihydroxyacetone phosphate) and greatly decrease the synthesis cost of rare sugars, a 'single kettle with four enzymes' strategy is adopted, the L-rhamnulose-1-phosphate aldolase derived from Thermotoga maritima MSB8 is used for synthesizing a series of rare sugars such as D-allulose, D- sorbose, L-fructose and L-tagatose. Experiments show that the aldolase has good thermal stability and can be used for highly selectively synthesizing D-allulose having important application value while D-glyceraldehyde is used as a receptor.

An object of the present invention is to provide a bakery product which has a soft and moist texture, sweetly dissolves in the mouth, has a favorable flavor, and is well risen and a method of producing the same. Another object of the present invention is to provide a method of producing the same. By using raw material flour obtained by blending a rare sugar-containing isomerized sugar syrup or a rare sugar in wheat flour as a main raw material in the production of a bakery product, a bakery product to which softness, moistness, and a property of sweetly dissolving in the mouth is imparted is produced.

[Problem] To provide a preservative solution for cells, tissues and organs using a rare sugar and a preservation method using the solution.

[Means for Resolution] A preservative solution for cryopreservation of animal or human organs and animal or plant tissues or cells, the solution containing a rare sugar D-allose. A preservative solution for cryopreservation of the human kidney, cells derived from animals or humans, or human or animal sperm and/or ovum and/or fermented ovum in which the cryopreservation is conducted at from −5 to 20° C. A preservative solution for cryopreservation of cells derived from animals or humans, or human or animal sperm and/or ovum and/or fermented ovum in which the cryopreservation is conducted at a temperature at which to start freezing to −196° C. A method for cryopreservation of an animal or human organ and an animal or plant tissue or cell at a low temperature, which comprises (a) refluxing or immersing a preservative solution for cryopreservation containing a rare sugar D-allose and the organ or the tissue or mixing the cell therewith, and (b) cooling the organ, the tissue or the cell to a low temperature of from −5 to 20° C.

The invention discloses a ribitol dehydrogenase (RDH) derived from Klebsiella oxytoca G4A4CGMCC No.7662, and realizes expression thereof in Escherichia coli. The experiment proves that the enzyme can realize the biotransformation production of allitol, and D-allulose can be converted into the functional rare sugar alcohol (allitol) with high conversion rate (96% or above at most) and less pollution; and an NADH (reduced nicotinamide adenine dinucleotide) regeneration system is added in the conversion process, and the NAD (nicotinamide adenine dinucleotide) consumption is low, thereby greatly lowering the cost. The allitol can be used in multiple fields such as preparation of pharmaceuticals (such as diabetes treatment medicaments and the like), health products and other raw materials of rare sugar and the like, and has wide application prospects.

[PROBLEMS] To find out a specific rare sugar having effects of inhibiting the proliferation of vascular endothelial cells and lumen formation and utilize these effects. To provide this rare sugar as a preventive/remedy for diseases with angiogenesis, a cosmetic or a functional food.

[MEANS FOR SOLVING PROBLEMS] A method of controlling the proliferation of vascular endothelial cells characterized by utilizing the vascular endothelial cell proliferation-controlling effect of D-mannose, D-allose, 2-deoxy-D-glucose, 3-deoxy-D-glucose, L-sorbose, 2-deoxy-D-ribose and/or 2-deoxy-L-ribose. A method of inhibiting lumen formation of vascular endothelial cells characterized by utilizing the vascular endothelial cell lumen formation-inhibiting effect of D-allose, D-altrose, D-gulose, D-talose, L-allose, 2-deoxy-D-glucose, 3-deoxy-D-glucose, D-ribose, L-ribose, 2-deoxy-D-ribose and/or 2-deoxy-L-ribose.

The invention provides an edible low-calorie rare sugar sweetening agent composition. Specially, trehalose, erythritol, neohesperidin dihydrochalcone and steviol glycoside are compounded in a certainproportion, so that a sweetening agent composition being good in homogeneity, low in calorie, good in flavor and good in stability can be obtained. The sweetening agent composition has the characteristics of being pure in mouth feel and refreshing, and also has the efficacy of being low in calorie, balanced in blood sugar and high in stability, containing prebiotics, preventing dental caries, andthe like.

The present invention provides functional rare sugar syrup and a preparation method thereof. A mass percentage of sucrose syrup in the rare sugar syrup is 0-99%. The rare sugar syrup is one or more of trehalose syrup, fructooligosaccharide syrup, allulose syrup, arabinose syrup, meso-erythritol syrup, neohesperidin dihydrochalcone syrup, stevioside syrup, maltooligosaccharide syrup, isomaltooligosaccharide syrup, galacto-oligosaccharide syrup, soybean oligosaccharide syrup, mixed cottonseed sugar, lactose and sucrose syrup, xylo-oligosaccharide syrup, mannan-oligosaccharide syrup, cyclodextrin glucose syrup, gentiobiose syrup, coupling sugar syrup, panose syrup, milk oligosaccharide syrup, chitin oligosaccharide acetylglucosamine syrup and ganoderma lucidum polysaccharide syrup. The functional rare sugar syrup has functions of protecting cells, regulating flora balance in body, regulating balances of blood sugar, blood pressure and blood lipids, protecting internal organs, promoting absorption of nutrients, preventing obesity, dental caries, oxidation and tumors, delaying aging, etc.

The invention discloses L-rhamnulose-1-phosphate aldolase and application thereof in catalytic synthesis of rare sugar D-psicose. The gene sequence of the L-rhamnulose-1-phosphate aldolase is obtainedby performing saturation site-directed mutagenesis of the 29th site, the 188th site, the 266th site or the 152nd site respectively on the amino acid of the wild type L-rhamnulose-1-phosphate aldolasecoded by SEQ ID NO.1. The invention overcomes the defect that wild type RhaD which produces D-psicose and D-sorbose in a ratio of 1 to 1 does not have stereoselectivity, the catalytic reaction only produces the D-psicose or takes the D-psicose as a main component, and the mutated RhaD has higher enzyme activity. By preparing the D-psicose from RhaD of rite-directed mutagenesis by the preparationmethod disclosed by the invention, the synthetic efficiency is high, the process is simple, the cost is reduced, and the maximum catalytic efficiency of the D-psicose reaches 100%; and the method disclosed by the invention is utilized for realizing synthesis of a lot of D-psicose by catalysis of D-glyceraldehyde by industrialized RhaD.

In the rare sugar strategy of Izumoring (FIG. 1), it is intended to establish a reaction system of producing rare sugars of many types by acquiring an isomerase which acts on various rare aldoses and, therefore, is most efficient in producing various rare ketoses. A DNA encoding the following protein (a) or (b). The above-mentioned DNA which is L-rhamnose isomerase derived from Pseudomonas stutzerii. A protein comprising the amino acid sequence represented by SEQ ID NO:2. A process for producing a recombinant protein characterized by culturing a host cell containing an expression system that can express the above-mentioned protein in a medium and collecting a recombinant protein having an L-rhamnose isomerase activity from the thus obtained culture. A method of applying FIG. 1 to the production of a rare sugar characterized in that the location of a target rare sugar in the overall picture of monosaccharides is understood and thus the optimum production pathway on which the above protein is allowed to act is designed.

The invention discloses a method for preparing a bird's-nest health protection product, and aims at providing a method for preparing the bird's-nest health protection product which is safe to eat andcan avoid the generation of lactulose based lysine and prevent proteins and amino acid therein from being damaged. The bird's-nest comprises the primary materials of 2-5 g of dry bird's-nest, 0-20 g of rare sugar and 1-100 ml of purified water, the bird's nest is stewed in a water bath heating manner to achieve the purpose of preventing the proteins and amino acid in the bird's-nest from being damaged under high-temperature stewing, the generation of toxic substances can be prevented, the eating is safer, the preserving time is longer, the portability is improved, and the eating is more convenient.

The present invention provides a method of avoiding an easy caramelization reaction and a Maillard browning reaction of rare sugar in food processing. Sodium alginate is used to bind whey protein powder with rare sugars to form granules, a glycoprotein structure is protected, the granules are resistant to a high temperature, the rare sugar can avoid the easy caramelization reaction and over-strong Maillard reaction of the rare sugar in the food processing and storing processes. In addition, surfaces of the granules are coated with a layer of sodium alginate, trehalose and calcium lactate membrane, the calcium lactate increases a strength of the sodium alginate and prevents a premature disintegration, L-arabinose or tagatose can be delivered to a designated gastrointestinal tract, in a function of human body acids, the granules are rapidly disintegrated to release the L-arabinose or tagatose and absorptions of the human body for sucrose and glucose are blocked. A protection function of the coating of the outer layer on the rare sugar is enhanced. The method can avoid the caramelization of the rare sugar at a low temperature and can also enable the rare sugar to bear a high temperature at 200 DEG C or more and to be enhanced in a performance of resisting the Maillard browning reaction.

The invention belongs to the technical field of biological fermentation and discloses complete equipment for producing xylitol and arabinose by directly utilizing a microbiological fermentation method. Corncob hydrolysate is adopted as a starting material, a high-tolerance yeast is taken as a carrier, and culture transformation is carried out through a primary seed culture fermentation tank and a secondary seed culture fermentation tank; xylose is transformed into xylitol under the conditions that the temperature is 36 DEG C and the pressure is 0.02MPa; and meanwhile, mannose, galactose and glucose in the hydrolysate are transformed into a biological oxidation-reduction enzyme and only -CHO in the xylose is catalyzed and transformed into -CH3OH. Effective transformation is carried out on all components of the corncob hydrolysate, industrial technology upgrading is carried out on a traditional product process, the obtaining mode of a rare sugar product is changed, the utilization rate of hydrolyzed components of the corncob is greater than 95%, the energy consumption and water consumption of a single product are reduced, waste residues and wastewater are greatly reduced, the quality of the product is improved and the cost of the product is reduced.