14 results about "Rare sugar" patented technology

Disclosed herein are formulations (including sweet proteins and rare sugars) for improving the sweetness of low glycemic index rare sugars without altering the texture and flavor of the rare sugars and methods for improving the sweetness of rare sugars. More specifically, the present invention discloses methods for improving the sweetness of rare sugars without altering the sweetness profile, texture and flavor of the primary sugars. The invention also discloses the addition of Brazitame protein with liquid rare sugars at different temperatures during the synthesis of the rare sugars, before crystallization or before or after caramelization of the rare sugars, to increase sweetness. The Brazilian sweet protein increases the sweetness of rare sugars, but is not limited to D-psicose, D-tagatose, D-allose, isomaltulose, trehalose, sorbose, pinose, leucobiose and the like. The invention also provides for the evaluation of non-nutritional sweeteners (such as aspartame, acesulfame, sodium cyclamate, neotame, saccharin, sucralose, stevioside, etc.), as each has a limitation of taste quality if used with rare sugars. However, the use of Brazilian sweet protein does not alter the flavor and texture of rare sugars, and the taste profile matches sucrose.

The present invention produces fermented kombucha beverages with reduced alcohol content by utilizing allulose or analogues thereof as the primary fermentable sugar. These rare sugars limit ethanol production during fermentation while preserving flavor, probiotic benefits, and acidity. The invention further encompasses the incorporation of Kratom (Mitragyna speciosa) extracts or alkaloids into the fermentation process or final product, enabling a low-alcohol kombucha with potential enhanced wellness properties. The resulting beverage maintains an alcohol content below 0.5% by volume, making it suitable for non-alcoholic labeling and broader consumer appeal. The invention is preferably a bottled beverage.

This invention relates to an immobilized enzyme membrane, its preparation method, and its application in the preparation of rare sugars, belonging to the field of enzyme catalysis technology. This invention prepares an immobilized enzyme membrane by controlling the surface charge of the membrane and then crosslinking it with RDPE and TMC. The positively charged membrane is more conducive to the loading of negatively charged RDPE. This immobilized enzyme membrane greatly improves the stability of the enzyme and enables its reuse and recovery. Free enzyme membranes and immobilized enzyme membranes were tested in positive osmosis mode. The immobilized enzyme membrane can achieve in-situ separation of products and can break the limitations of reaction equilibrium.

The invention discloses a recombinant ribitol dehydrogenase strain and application thereof, and belongs to the technical field of bioengineering. According to the recombinant ribitol dehydrogenase strain disclosed by the invention, pichia pastoris GS115 is taken as a starting strain, a recombinant ribitol dehydrogenase carrier is transformed, and the ribitol dehydrogenase gene sequence is shown as SEQ ID. NO 1. After fermentation in a 50L fermentation tank, the fermentation liquor is centrifuged, the supernate contains recombinase RDH with the purity of 95% or above, and the enzyme activity can reach 1059U / mL. The recombinase catalyzes a redox reaction between D-psicose and D-psilol to produce D-psilol, and the conversion rate can reach 56% after the reaction is balanced. The enzyme has good heat resistance, does not need metal ions to participate in a catalytic reaction, is low in product separation and purification difficulty, can keep the maximum enzyme activity of 80% or above in a wide pH (6-9) range, and can be applied to large-scale industrial production of rare sugar D-psilol.

The invention relates to the technical field of food, and provides a rare sugar composition and a preparation method thereof, and the rare sugar composition comprises tagatose and psicose in a mass ratio of (40-98): (2-10). The preparation method comprises the following steps: by taking lactose hydrolysate as a raw material, adding calcium hydroxide into the lactose hydrolysate for reaction, wherein the reaction product comprises the rare sugar composition. According to the rare sugar composition, the rare sugar is high in purity and clean and pure in flavor, the sweetness and taste of the rare sugar are close to those of cane sugar, the rare sugar composition can serve as a substitute of cane sugar, according to the preparation method of the rare sugar composition, lactose serves as a raw material and is isomerized into tagatose and psicose, the conversion rates of the tagatose and the psicose can reach 40% and 4% respectively, reaction conditions are mild, the preparation process is simple and controllable, and the preparation cost is low. And the production process has flexibility, rare sugar mixtures with different components and different specifications can be obtained by combining separation and purification process adjustment, different production processes can be selected according to actual requirements of products, and the method has a good industrial application prospect.

The present invention discloses a process for separating and purifying sugar component from the ternary mixture of monosaccharides and / or disaccharides using continuous ternary separation chromatography system with variable length chromatography zones. The present invention particularly discloses a process for production of allulose or sucrose isomer using ternary separation chromatography system with variable length chromatography zones. The present invention is an improvement over the conventional method which fails to separate ternary sugar mixtures comprising of monosaccharaides and / or disaccharides.

The invention provides a property-changed fructose-1, 6-diphosphate aldolase mutant and application thereof, and relates to the technical field of enzyme engineering. According to the invention, a series of enzyme mutants with enhanced structural stability are obtained by carrying out rational design and site-directed mutagenesis on key sites of wild fructose-1, 6-diphosphate aldolase. Compared with a wild type, the mutant can keep certain enzyme activity at high temperature, shows excellent heat resistance, can be used as a core biocatalyst to efficiently catalyze a chiral compound synthesis reaction taking dihydroxyacetone phosphate as a key precursor, can still keep high catalytic efficiency at 50 DEG C, and can be used for preparing a chiral compound. The key bottleneck that natural enzyme is prone to thermal inactivation in industrial application is solved, and the method has wide application prospects in green biological manufacturing of high-added-value products such as drug intermediates and rare sugar.

The invention discloses a D-tagatose-3-epimerase mutant and application thereof, the D-tagatose-3-epimerase mutant has a single-point mutation or a multi-point mutation in the 45th mutation, the 48th mutation, the 50th mutation, the 80th mutation, the 93th mutation, the 98th mutation and the 144th mutation relative to an amino acid sequence SEQ ID NO.1 of a wild type D-tagatose-3-epimerase, and the D-tagatose-3-epimerase mutant has a single-point mutation or a multi-point mutation in the 45th mutation, the 48th mutation, the 50th mutation, the 80th mutation, the 93th mutation, the 98th mutation and the 144th mutation. The invention also provides a corresponding coding gene, a recombinant vector, a genetically engineered bacterium, a biocatalyst and application in preparation of rare sugars. The invention develops the D-tagatose-3-epimerase mutant which has stronger adaptability with glucose isomerase and is more stable in a cascade reaction, is beneficial to improving the efficiency of reaction for preparing rare sugar by using xylose, can simultaneously consider higher substrate concentration and D-xylose conversion rate, and reduces the production cost of the product.

The application discloses a kind of added Co 2+ The application discloses a method for improving the yield of D-allulose produced by multi-enzyme catalysis, belonging to the field of biotechnology. The application provides a method for producing D-allulose by multi-enzyme catalysis, which comprises adding sucrose phosphorylase from Bifidobacterium adolescentis, fructokinase from Clostridium acetobutylicum, D-allulose-6-phosphate phosphatase from Clostridium thermocellum, and D-allulose-6-phosphate-3-epimerase from Pantoea sp to a reaction system containing sucrose and ATP, and then adding a metal ion solution to the reaction system to produce D-allulose. The application synthesizes an important low-calorie functional rare sugar D-allulose using inexpensive sucrose as a substrate, and experiments show that the addition of cobalt ions can increase the yield of D-allulose by five times.

The technology disclosed in this specification pertains to methods of obtaining a rare from a source solution comprising a source sugar. The method uses a single ionic moiety that can be removed from a mixed sugar solution comprising the source sugar and rare sugar without use of ion exchange before chromatographic separation.

The present invention provides a cosmetic composition that can maintain high moisturizing properties even after being washed off with water. [Solution] The cosmetic composition of the present invention contains a biosurfactant having a mannose skeleton and a water-retaining agent. For example, the water-retaining agent is a sugar alcohol, rare sugar, isomerized sugar, alkyl glycoside, sucrose fatty acid ester, or hyaluronic acid and derivatives thereof, and the biosurfactant contains mannosylerythritol lipid (MEL).

The present invention aims to provide a food (including foods for specified health uses and foods with functional claims) or pharmaceutical product containing rare sugars as an active ingredient that can be used for novel applications. [Solution] The above problem is achieved by a food composition or pharmaceutical composition containing D-allose, D-sorbose, or derivatives thereof as an active ingredient, for the prevention, improvement, or treatment of symptoms or diseases selected from the group consisting of obesity due to overeating, diabetes or elevated blood glucose levels resulting from obesity due to overeating, and hepatic fat accumulation resulting from obesity due to overeating.

To provide a method for producing calcium-containing rare sugar syrup in the presence of commonly found shells or eggshells by using reducing sugar as a raw material and by microwave heating treatment.SOLUTION: In the presence of shells or eggshells, reducing sugar is heated by microwave under pressure to efficiently isomerize into rare sugar, and thus various kinds of calcium-containing rare sugar syrup can be produced.SELECTED DRAWING: None

The present technology provides a beverage composition that includes about 0.005 wt % to about 0.06 wt % of a high potency sweetener, about 0.005 wt % to about 0.75 wt % of a hydrocolloid, and about 1 wt % to about 5 wt % of a rare sugar. The beverage composition may be a zero or reduced calorie sweetened beverage composition, which mimics taste normally delivered from sucrose.