Application of L-rhamnulose-1-phosphate aldolase in catalytic synthesis of rare sugars

Abstract

The invention discloses application of L-rhamnulose-1-phosphate aldolase in catalytic synthesis of rare sugars. In order to avoid the direct use of DHAP (dihydroxyacetone phosphate) and greatly decrease the synthesis cost of rare sugars, a 'single kettle with four enzymes' strategy is adopted, the L-rhamnulose-1-phosphate aldolase derived from Thermotoga maritima MSB8 is used for synthesizing a series of rare sugars such as D-allulose, D- sorbose, L-fructose and L-tagatose. Experiments show that the aldolase has good thermal stability and can be used for highly selectively synthesizing D-allulose having important application value while D-glyceraldehyde is used as a receptor.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of L-rhamnosan-1-phosphate aldolase in catalyzing the synthesis of rare sugars. Background technique [0002] Rare sugars are defined as monosaccharides and their derivatives that occur in nature but in very low amounts. Rare sugars generally have a sweetness similar to sucrose, but yield few or fewer calories. Rare sugars have broad application prospects in food, medicine, health care and other fields. Taking D-psicose as an example for illustration, D-psicose is a six-carbon sugar with a very low content in nature and is an epimer at the C-3 position of D-fructose. D-Psicose is difficult to digest and absorb, and hardly provides energy for life activities, so it is a very useful low-calorie sweetener. [0003] In the field of food applications, D-psicose has the advantages of high sweetness, good solubility, low calories and low blood sugar response, and is consi...

AI Technical Summary

Problems solved by technology

At present, the synthetic routes of most rare sugars are still limited, and relatively expensive compounds are often used as substrates, resulting in the high cost of synthesis of rare sugars

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