43 results about "Dihydroxyacetone" patented technology

The invention relates to the field of fine chemical materials, and realizes a low-cost and high-efficiency preparation method of 1, 3-dihydroxyacetone by taking acetone as a raw material through two steps of halogenation reaction and alkali substitution reaction for the first time. The whole process is simple to operate, green and environment-friendly.

The application provides a BFD mutant and application thereof as a carbon chain lengthening enzyme, and comprises: (a) the BFD mutant contains at least one site of L43, A73, T100 and K165 compared with the amino acid sequence shown in SEQ ID NO. 1, and has an amino acid sequence which is subjected to amino acid mutation and has a carbon chain lengthening enzyme activity function; or (b) the BFD mutant has an amino acid sequence which is subjected to substitution, deletion and / or addition of one or more conservative amino acid residues and has the same function. The enzyme activity of the BFD mutant is obviously improved, and the yield and conversion rate of formaldehyde to ethanol aldehyde and / or 1,3-dihydroxyacetone are improved.

The invention relates to the technical field of glass processing, in particular to food-grade cutting fluid for glass processing and a preparation method of the food-grade cutting fluid. The lubricating oil is prepared from the following raw materials in percentage by weight: 50-60% of food-grade base oil, 10-15% of a food-grade lubricant, 3-8% of a food-grade auxiliary stabilizing material and the balance of water, the food-grade auxiliary stabilizing material is composed of a 1, 3-dihydroxy acetone dimer, an emulsion stabilizer, an adsorption stabilizer and an enhancement and protection stabilizer; the emulsion stabilizer is emulsified calcium stearate dispersion liquid and / or sodium polyphosphate; the adsorption stabilizer is a composition of multiple of magnesium trisilicate, food-grade diatomite and food-grade activated clay; the reinforcing and protecting stabilizer is calcium alginate and / or food-grade titanium dioxide, food-grade raw materials are adopted, and the glass cutting agent has a good cutting effect and good quality stability when being used for glass processing.

The invention discloses a protein fiber dyeing method, a protein fiber and a fabric, according to the protein fiber dyeing method, reducing sugar DHA is reacted with indigo blue, and low-temperature green dyeing and functional modification of the protein fiber are synchronously realized by utilizing the dual functions of a reducing agent and a color developing agent of DHA. Specifically, ketose-dihydroxy acetone (DHA) is used for reducing indigo into a leuco body, meanwhile, the ketose-dihydroxy acetone (DHA) and amino in protein fibers are subjected to a Maillard reaction to generate yellowish-brown pigment, and a unique yellow-green system is formed by combining a blue system of traditional indigo, so that the dyeing chromatography is widened, meanwhile, the dyeing process is green and environmentally friendly, and the dyeing efficiency is improved. The dyed protein fiber is endowed with excellent anti-ultraviolet performance (UPFgt; 1800) and oxidation resistance (the free radical scavenging rate can reach 70%), and the strength of the dyed protein fiber is almost not lost after dyeing.

This invention relates to the field of hydrogen production technology, specifically to a method for producing dihydroxyacetone from glycerol via electrochemical oxidation coupled with hydrogen production. The method includes: S1 preparing a glycerol alkaline solution as the electrolyte solution, the purity of which must reach analytical grade; S2 placing the electrolyte solution prepared in S1 into an electrolytic cell, connecting the positive terminal of a power supply to the anode in the electrolytic cell, and connecting the negative terminal of the power supply to the cathode in the electrolytic cell; S3 turning on the power supply and, at a defined temperature, performing a redox reaction and a hydrogen evolution reaction to obtain dihydroxyacetone prepared by anodic oxidation, and simultaneously obtaining hydrogen gas prepared by cathode hydrogen evolution. This patent constructs a synergistic reaction system of selective anodic oxidation to dihydroxyacetone and efficient cathode hydrogen evolution by using a glycerol alkaline solution as the electrolyte solution. The glycerol alkaline solution weakens the nucleophilic attack intensity of hydroxide ions and inhibits carbon-carbon bond breaking through the in-situ protection mechanism of the glycerol carbon chain, achieving highly selective and directional synthesis of dihydroxyacetone.

The invention provides a carbon chain elongase as well as a coding gene and application thereof, the amino acid sequence of the carbon chain elongase is as shown in a sequence table SEQ ID NO.1, the nucleotide sequence of the gene for coding the carbon chain elongase is as shown in a sequence table SEQ ID NO.2, and the carbon chain elongase can be used as a formaldehyde lyase and has a function of efficiently catalyzing formaldehyde to synthesize 1, 3-dihydroxyacetone.

The application discloses a formaldehyde lyase mutant and application thereof. The formaldehyde lyase mutant provided by the application can regulate the generation proportion of a two-carbon and three-carbon product generated by catalyzing formaldehyde condensation, has improved activity of catalyzing formaldehyde condensation into glycolaldehyde, and reduced activity of catalyzing formaldehyde condensation into 1,3-dihydroxyacetone, compared with formaldehyde lyase.

The invention relates to a preparation method and application of a phosphorus-doped lignin-derived carbon-loaded NiCu alloy material. The preparation method comprises the following steps: preparing phosphorized lignin by phosphorizing a plurality of pairs of lignin, taking the phosphorized lignin as a phosphorus source and a carbon source, regulating pH to enable the phosphorized lignin to be coordinated and combined with Ni and Cu ions to form a nickel / copper-lignin-based supramolecular precursor, and then performing high-temperature carbon burning to obtain the nickel / copper-lignin-based supramolecular composite material. The catalyst combines the advantages of a biomass carbon material and transition metals Cu and Ni, is a catalyst with relatively excellent performance, can be widely applied to electrocatalytic conversion of glycerol, furfural, glucose and other biomass micromolecules into high-value chemicals, and particularly has an excellent effect on conversion of glycerol into a high-value product dihydroxyacetone. The construction of a P-C structure on the carrier is beneficial to the local enrichment of active oxygen groups, the generation of metal active sites is promoted, the oxidation rate of glycerol is accelerated, and NiCu alloying obviously improves the selectivity of dihydroxyacetone. The catalyst is stable in effect, rich in resources and low in price.

The present application discloses a mutant Asaia bogorensis strain (Deposition number DSM 34962) as well as compositions, cell cultures and kits comprising said strain. The present application also discloses the use of said Asaia bogorensis strain, compositions, cell cultures or kits in methods of producing dihydroxyacetone (DHA).

The invention relates to a dihydroxyacetone phosphate transaminase CJ1361 mutant with improved stability, and a gene recombinant bacterium containing the dihydroxyacetone phosphate transaminase CJ1361 mutant and an enzyme liquid of the gene recombinant bacterium as biocatalysts, which are used for synthesizing serinol by a microbial fermentation method. Compared with the wild type dihydroxyacetone phosphate transaminase CJ1361, the yield of serinol produced by the dihydroxyacetone phosphate transaminase CJ1361 mutant provided by the invention is remarkably improved.

The present invention relates to a process for producing a foam, which comprises foaming a mixture, comprising one or more amino acid copolymers (A), one or more components (B) capable of reacting with said amino acid copolymers (A) and one or more blowing agents (F), wherein component (B) is selected from alpha-hydroxy ketones and alpha-aldehydes such as reducing sugars, 1,3-dihydroxyacetone, glycolaldehyde, glyceraldehyde or any mixture thereof and the foam obtainable by this process.

The invention discloses a method for producing dihydroxyacetone (DHA) through selective photoelectric oxidation electrolysis of glycerol. The method comprises the following steps: preparing a phosphate buffer solution containing H2PO4 <-> and / or HPO4 < 2->; adding glycerol into the phosphate buffer, and uniformly mixing to obtain an electrolyte; and carrying out photoelectric oxidation electrolysis on the electrolyte by adopting a working electrode made of TiO2 so as to obtain the product DHA. The production process is simple and convenient, and is especially suitable for industrial large-scale efficient production of DHA.

The invention belongs to the technical field of chemical catalysis, and particularly relates to a method for preparing dihydroxyacetone by directionally catalyzing and oxidizing glycerol based on a hairpin structure. Comprising the following steps: taking a conductive substrate loaded with a catalyst Ce-coated Fe2O3 as a photo-anode, and assembling the conductive substrate, a cathode and an electrolyte into a photoelectric catalytic reaction device; and adding glycerol and glutaraldehyde into the electrolyte, and selectively oxidizing the glycerol into dihydroxyacetone under the conditions of illumination and voltage application. According to the invention, hydrogen bond acceptor molecule glutaraldehyde with a specific spatial configuration is selected to form a hairpin composite structure with hydroxyl groups at two ends of glycerol, and accurate activation and directional oxidation of intermediate hydroxyl groups are realized under the action of a catalyst Ce-coated Fe2O3, so that glycerol is directionally and efficiently oxidized into 1, 3-dihydroxyacetone, and the yield of 1, 3-dihydroxyacetone is improved. And an innovative solution is provided for high-selectivity conversion of the biomass-derived polyol.

This invention relates to the field of plant disease control technology, and in particular to the application of dihydroxyacetone (DHA) in the control of root rot and / or the promotion of plant growth. This invention found that DHA can effectively antagonize the growth of the pathogen *Fusarium graminearum*, with an inhibition rate of 36.14%–48.65%. In soil culture experiments, at 7 days, both high concentrations (1M) and low concentrations (5mM) of DHA showed good control effects on maize root rot, with disease control efficacy reaching 14.17% and 27.08%, respectively. Simultaneously, both showed good growth-promoting effects; low concentrations of DHA increased the aboveground biomass of maize by 65.30% compared to diseased plants. At 14 days, the control efficacy of high concentrations (1M) and low concentrations (5mM) of DHA against maize root rot was 15.24% and 33.05%, respectively, and low concentrations of DHA increased the aboveground biomass of maize by 135.32% compared to diseased plants.

The invention discloses a water-based zinc ion battery electrolyte containing 1, 3-dihydroxyacetone and a preparation method and application thereof. The water-based zinc ion battery electrolyte comprises a zinc salt, an additive and a solvent, the additive is 1, 3-dihydroxy acetone. 1, 3-dihydroxy acetone is introduced into the zinc sulfate electrolyte of the water-based zinc ion battery to inhibit dendritic growth of a zinc anode, an EDL structure at a zinc anode interface is adjusted, and the cycle life of the water-based zinc ion battery is prolonged. The 1, 3-dihydroxy acetone serving as a bifunctional group can improve ion diffusion and nucleation kinetics on a zinc interface, so that uniform electrodeposition of zinc is realized. Besides, HER can be inhibited by replacing water molecule groups around zinc ions, and 1, 3-dihydroxyacetone is used as an electrolyte additive of the zinc ion battery, so that zinc foil corrosion and zinc dendrite growth are effectively inhibited, the stability and reversibility of a zinc anode in the deposition or stripping process are remarkably improved, and the service life of the zinc anode is prolonged. Therefore, the water-based zinc ion battery has excellent electrochemical performance and long cycle stability.

The invention discloses a LysR type transcriptional regulatory factor knockout Gluconobacter sp. And application thereof in continuous catalysis of DHA production, and belongs to the technical field of bioengineering. In order to improve the yield of DHA (1, 3-dihydroxyacetone), the expression levels of genes under different fermentation processes of batch fermentation and continuous catalytic perfusion are analyzed and compared through transcriptome, the relationship between the gene expression and the yield of 1, 3-dihydroxyacetone is explored, differential genes in the relationship are analyzed and verified, and it is found that after a LysR type transcriptional regulation factor is knocked out, the expression level of the 1, 3-dihydroxyacetone is analyzed and verified. And the yield of the 2, 3-dihydroxyacetone is obviously improved. After 12 days of continuous catalytic perfusion fermentation production, the cumulative yield of 1, 3-dihydroxyacetone is improved by 41.92% compared with the cumulative yield of 1, 3-dihydroxyacetone of a control strain, and the glycerin conversion rate is improved by 12.58% compared with the glycerin conversion rate of the control strain. Metabolism of Gluconobacter is changed by knocking out a LysR type transcriptional regulation factor, so that synthesis of 1, 3-dihydroxyacetone is influenced.

The invention provides a method for biosynthesizing acrylic acid from methanol. The method comprises the following steps: introducing carbon-compound methanol into an acrylic acid synthesis route, catalyzing methanol and oxygen to generate formaldehyde through methanol oxidase, and catalyzing formaldehyde condensation into 1, 3-dihydroxyacetone through artificial formaldehyde condensation enzyme; 1, 3-dihydroxy acetone is catalyzed by dihydroxy acetone kinase to be subjected to phosphorylation to generate dihydroxy acetone phosphate, and the dihydroxy acetone phosphate is catalyzed by CEAS to synthesize acrylic acid. According to the invention, 10 kinds of CEAS from different sources are also excavated, and 3-phosphoglyceraldehyde or phosphoric acid-1, 3-dihydroxy acetone can be catalyzed to be converted into acrylic acid. By utilizing the enzyme with the highest activity, cheap methanol can be converted into acrylic acid only through four steps of enzymatic cascade reaction, thermodynamics is beneficial, and high-yield and high-yield acrylic acid can be obtained without a strict environment.

The invention provides a property-changed dihydroxyacetone kinase mutant and application thereof, and relates to the technical field of enzyme engineering. Through rational design and molecular modification, wild dihydroxyacetone kinase is subjected to single-point mutation and combined mutation, a series of enzyme mutants with enhanced thermal stability are obtained, and the catalytic activity of the mutants under the high-temperature condition is remarkably superior to that of wild enzymes. The invention further provides a multienzyme coupling reaction system containing the dihydroxyacetone kinase mutant, and the multienzyme coupling reaction system is used for efficiently catalyzing the process of synthesizing lactic acid by taking dihydroxyacetone as a substrate. The thermostable dihydroxyacetone kinase mutant provided by the invention solves the key bottleneck that a wild enzyme is easy to thermally inactivate under industrial application conditions, and has important industrial application prospects.

The application provides the field of electronic cigarettes, in particular to an atomization base liquid, an atomization liquid and a method for reducing small particle size aerosol particles, the atomization base liquid comprising one or more combinations of propylene glycol, glycerol, water and ethanol, characterized in that it further comprises 1,3-dihydroxyacetone, wherein the mass fraction of 1,3-dihydroxyacetone is 5-50%. The atomized particles of the electronic cigarette aerosol containing the atomization base liquid will be concentrated in two particle size ranges; the small particle size aerosol can make the smoker quickly satisfied, the amount of smaller particle size aerosol is reduced, and the influence on alveoli is reduced. The large particle size aerosol can bring better taste to the smoker.

The invention discloses a saccharomyces cerevisiae genetically engineered bacterium for efficiently synthesizing mogroside V through multi-dimensional cytoplasm adaptability and a construction method of the saccharomyces cerevisiae genetically engineered bacterium. The construction method of the saccharomyces cerevisiae genetically engineered bacterium comprises the following steps: integrating truncated 3-hydroxy-3-methylglutaryl coenzyme A reductase tHMG1, pentenyl pyrophosphate isomerase IDI1, squalene synthetase ERG9, epoxy squalene cyclization enzyme ERG1, choline kinase ScCK, isopentenyl phosphokinase AtIPK, phosphoketolase BbPK, phosphotransacetylase PTA and dihydroxyacetone hydrolase EcHAD at a site of a saccharomyces cerevisiae genome GAL80, and carrying out enzymolysis, so as to obtain the saccharomyces cerevisiae genetically engineered bacterium. The invention relates to a preparation method of a cytochrome P450 enzyme, which comprises the following steps of: preparing a cycloepoxide hydrolase SgEPH3, a cytochrome P450 enzyme CYP87D18 and a cytochrome P450 enzyme reductase AtCPR1; an ABC efflux protein PDR11, a glycosyl transferase UGTMG1, a sucrose synthase Susy and a glycosyl transferase SgUGT94-289-3 are integrated at a site Exg1 of a saccharomyces cerevisiae genome. The saccharomyces cerevisiae genetically engineered bacterium constructed by the invention realizes a higher level of mogroside V yield synthesized from the beginning in microorganisms, and has important industrial application potential.

The invention provides a carbon chain elongase as well as a coding gene and application thereof, the amino acid sequence of the carbon chain elongase is as shown in a sequence table SEQ ID NO.1, the nucleotide sequence of the gene for coding the carbon chain elongase is as shown in a sequence table SEQ ID NO.2, and the carbon chain elongase can be used as a formaldehyde lyase and has a function of efficiently catalyzing formaldehyde to synthesize 1, 3-dihydroxyacetone.

The invention relates to the technical field of animal model establishment, and discloses a method for establishing an animal model with abnormal skin pigments. The construction method comprises the steps of: providing an animal having skin without hair coverage and a drug solution containing 1, 3-dihydroxyacetone (DHA) and a penetration enhancer; loading the medicine solution on the skin, which is not covered by hair, of the animal, and then carrying out ultraviolet radiation; and waiting for 8-16 hours to obtain the animal model with abnormal skin pigment. According to the construction method, the melanin-like synthesis and accumulation effect caused by DHA can be enhanced in a short time, the modeling time is remarkably shortened, and skin injuries such as erythema and blisters are reduced. Compared with a traditional ultraviolet radiation method, the model success rate is higher, operation is simpler and more convenient, stable and uniform chromatosis can be formed, the method is suitable for standardized evaluation and research of skin disease treatment schemes, and a more efficient animal model is provided for treatment research of skin pigment abnormality diseases.

The application discloses mutant protein of phosphonacetyltransferase and application. The application provides mutant protein of phosphonacetyltransferase, residues at positions 21, 36, 42, 49, 64, 82, 150 and 244 in the amino acid sequence shown in sequence 1 are mutated to obtain protein with phosphonacetyltransferase activity; and the synthesis capacity of 1,3-dihydroxyacetone is improved. The application also provides related biological materials and derivatives of the protein and application of the protein in preparation of 1,3-dihydroxyacetone. The 1,3-dihydroxyacetone yield of the protein is greatly improved, the mutant SF6-11 of phosphonacetyltransferase is compared with wild-type phosphonacetyltransferase as a starting material, the 1,3-dihydroxyacetone yield in flask fermentation is increased by 1.65 times, and the 1,3-dihydroxyacetone yield is increased from 2.75+ / -0.07 g / L to 4.54+ / -0.09 g / L.

The application discloses aniline molecular modified semiconductor photoanode and a preparation method and photoelectrocatalytic glycerol oxidation application thereof; the photoanode takes WO3, TiO2 or BiVO4 and the like grown on a conductive glass (FTO) substrate as a base material, and then grafts an aniline monomer molecule at a molecular level on the surface of the base material; the modification method can not only optimize the electronic structure of the base photoanode material to obtain higher photo-generated electron-hole pair migration efficiency and survival time, but also form steric hindrance to affect the adsorption state of glycerol molecules on the surface of the photoanode material, so that the selectivity is improved; the improved photoanode material of the application shows excellent catalytic activity and stability in the application of photoelectrocatalytic glycerol oxidation into glycerol aldehyde, glyceric acid, dihydroxyacetone and other high-value-added three-carbon products.

The invention discloses a preparation method of a recyclable heat-conducting water-based self-repairing polyurethane elastomer. Isophorone diisocyanate, polytetrahydrofuran and dimethylolpropionic acid are used as main agents, dynamic imine bonds are constructed through dihydroxy acetone and adipic acid dihydrazide capable of forming high-density hydrogen bonds, two-dimensional polyamide is used as filler, and the waterborne polyurethane elastomer which is excellent in self-repairing performance and mechanical performance, high in heat conductivity coefficient and recyclable is obtained. The synthesis method is simple, the compatibility of two-dimensional polyamide and polyurethane is good, and the mechanical property is excellent; the damaged elastomer can be repaired under the room temperature condition, the repairing cost is low, energy is saved, and the method is environmentally friendly and safe. Meanwhile, the elastomer is easy to recycle, has sustainability, and can be widely applied to the fields of flexible electronic products and the like which need to have good heat-conducting property and mechanical property at the same time.

The present invention discloses a method for improving 1,3-dihydroxyacetone yield and production intensity of Gluconobacter oxydans, and belongs to the field of fermentation engineering technology. The present invention enhances the efficiency of converting its substrate glycerol into 1,3-dihydroxyacetone by knocking out the dehydrogenase gene that has a potential impact on the metabolic flux of 1,3-dihydroxyacetone in Gluconobacter oxydans, thereby improving the yield and production intensity of 1,3-dihydroxyacetone. Compared with the control strain G.oxydansWSH-003, the 1,3-dihydroxyacetone yield, conversion rate and production intensity of recombinant strains G.oxydans WSH-1, G.oxydans WSH-2, G.oxydans WSH-3, G.oxydans WSH-4, G.oxydans WSH-5, G.oxydans WSH-6, G.oxydans WSH-7 and G.oxydans WSH-8 are significantly improved.

The invention provides a preparation method of bio-based tromethamine, which comprises the following steps: S1, taking bio-based 1, 3-dihydroxy acetone, hydrogen peroxide and an ammonia source as raw materials, and carrying out oxidation nitration reaction under the action of a catalyst to obtain 1, 3-dihydroxy nitropropane; and S2, adding a hydroxymethylation reagent into the 1, 3-dihydroxy nitropropane, carrying out condensation reaction under an alkaline condition, and carrying out hydrogenation reduction to obtain the bio-based tromethamine. According to the invention, a sustainable synthesis route based on bio-based raw materials is developed, renewable 1, 3-dihydroxy acetone which can be prepared through microbial fermentation is taken as a starting point, and the bio-based tromethamine is efficiently prepared through an optimized three-step method of oxidation nitration, condensation and reduction. According to the route, the reaction efficiency and selectivity are remarkably improved, the raw material cost and the process environment influence are effectively reduced, and a solid foundation is laid for green and economic production of bio-based tromethamine.

The application discloses a melanin promoting composition and a preparation method and application thereof, and belongs to the technical field of cosmetics and biological agents. The melanin promoting composition is composed of the following components in percentage by mass: acetyl hexapeptide-1 0.01-0.5 parts, Poria cocos extract 1-5 parts, sophorolipid 0.5-5 parts, 1,3-dihydroxyacetone 3-20 parts, a humectant 5-20 parts, a preservative 0.1-0.5 parts, a thickening agent 0.1-1 part, a pH regulator 0.1-0.3 parts, an emulsifier 0.5-3 parts, and the rest is water. The composition selects a small-molecule peptide with biological activity, and is compounded with sophorolipid, 1,3-dihydroxyacetone, Poria cocos extract and a humectant to realize the functions of promoting tanning, accelerating tanning, prolonging tanning time, avoiding dry skin color and unevenness in the natural tanning process, and strong stability in long-time storage.

Cosmetic compositions comprising a DHA-derived polymer, a method for applying this polymer, and a synthesis method. The present invention relates to the use in cosmetics of a polymer obtained from 1,3-dihydroxyacetone (DHA). The invention also relates to a method for synthesizing this copolymer and to a monomer used as a reagent in this method. Finally, the invention relates to a method for applying this polymer to the skin. Figure for the abstract: Fig. 1.

The invention provides a method for separating 1, 3-dihydroxyacetone and glycerol. The method provided by the invention comprises the step of performing multi-stage extraction on a raw material solution containing 1, 3-dihydroxyacetone and glycerol by using a hydrophobic extraction agent, wherein a solvent in the raw material solution comprises water; the hydrophobic extraction agent comprises a hydrophobic ionic liquid and / or a hydrophobic polar organic solvent. The method provided by the invention has a better separation effect on 1, 3-dihydroxyacetone and glycerol with similar structures, and the two separated substances have higher yield and purity.