73 results about "Dihydroxyacetone" patented technology

The invention provides formaldehyde lyase as well as a mutant and application thereof. The amino acid sequence of the formaldehyde lyase mutant is shown as SEQ ID NO.1, SEQ ID NO.4 or SEQ ID NO.6 and contains at least one of the following sites: S26, W86, N87 or Y87, L109 or M109, L110, H281, Q282 or E282 and A460, and the amino acid sequence is subjected to amino acid mutation and has a formaldehyde lyase activity function. The amino acid sequences of the formaldehyde lyase and the mutants of the formaldehyde lyase have at least 99% of sequence identity with SEQ ID NO.1-7. The formaldehyde lyase and the mutant thereof can catalyze formaldehyde to synthesize 1, 3-dihydroxyacetone, and a new way for converting formaldehyde into 1, 3-dihydroxyacetone is provided.

The invention discloses mutant protein of dihydroxyacetone phosphate phosphatase and application of the mutant protein. The invention provides a phosphodihydroxyacetone phosphatase mutant protein, which is characterized in that residues of phosphodihydroxyacetone phosphatase corresponding to 21st, 36th, 42nd, 49th, 64th, 82nd, 150th and 244th sites of an amino acid sequence shown in a sequence 1 are mutated to obtain a protein with phosphodihydroxyacetone phosphatase activity; and the synthesis capability of the 1, 3-dihydroxyacetone is improved. The invention also provides related biological materials and derivatives of the protein, and application of the protein in preparation of 1, 3-dihydroxyacetone. The yield of 1, 3-dihydroxy acetone of the protein is greatly increased, compared with starting wild type dihydroxy acetone phosphate phosphatase, the yield of 1, 3-dihydroxy acetone obtained through shake flask fermentation of the dihydroxy acetone phosphate phosphatase mutant SF6-11 is increased by 1.65 times, and the yield of 1, 3-dihydroxy acetone is increased to 4.54 + / -0.09 g / L from 2.75 + / -0.07 g / L.

The invention discloses a production method of 1, 3-dihydroxy acetone. The invention provides a method for constructing an engineering strain capable of producing 1, 3-dihydroxyacetone by using glucose, which comprises the following steps: A, inhibiting the expression of the following endogenous gene A on the basis of recipient bacteria, and enabling the recipient bacteria to express the coding gene of T7RNA polymerase T7RNAP and the coding gene of 1, 3-dihydroxyacetone phosphatase HdpA; in a 5L tank fermentation test for producing 1, 3-dihydroxyacetone by using glucose, the yield of the 1, 3-dihydroxyacetone can reach 30.1 g / L, and the conversion rate from the glucose to the 1, 3-dihydroxyacetone can reach 0.95 mol / mol. The method disclosed by the invention is of great significance to synthesis of 1, 3-dihydroxyacetone from glucose.

The invention provides a BFD mutant and application thereof as a carbon chain elongase, and the BFD mutant is characterized in that (a) compared with an amino acid sequence as shown in SEQ ID NO.1, the BFD mutant contains at least one of the following sites: L43, A73, T100 and K165, and an amino acid sequence which is subjected to amino acid mutation and has an active function of the carbon chain elongase; or (b) an amino acid sequence which is obtained by substitution and / or deletion and / or addition of one or more conserved amino acid residues of the BFD mutant and the amino acid sequence shown in (a) and has the same function. The enzyme activity of the BFD mutant is obviously improved, and the yield and conversion rate of glycolaldehyde and / or 1, 3-dihydroxyacetone generated from formaldehyde are improved.

The invention discloses a cocatalyst / heterostructure / tungsten trioxide photo-anode integrated structure and a preparation method and application thereof, and belongs to the technical field of biomass conversion and photoelectrocatalysis. The method comprises the following steps: firstly, synthesizing a zinc oxide / tungsten trioxide heterostructure by adopting hydrothermal, spin-coating and calcining modes, then synthesizing a noble metal modified nickel phosphide cocatalyst through phosphorization, and loading the cocatalyst on the surface of the heterostructure to successfully construct a cocatalyst / heterostructure / photo-anode integrated system. The heterostructure of zinc oxide and tungsten trioxide can inhibit recombination of photo-generated carriers, and the noble metal modified cocatalyst can reduce interface resistance between the heterostructure and the electrolyte, accelerate migration of photo-generated holes, greatly reduce surface energy barriers, improve surface reaction kinetics and prolong the service life of photo-generated charges. The method has the advantages that the method is simple and easy to implement, excellent photoelectrocatalysis glycerol oxidation performance is achieved, only two C3 products including glyceraldehyde and 1, 3-dihydroxyacetone are available, the variety is few, and a feasible path is provided for improving photoelectrocatalysis biomass high-valued coupling hydrogen production.

The present invention relates to a method for photocatalytically decomposing dihydroxyacetone to produce carbon monoxide and ethylene glycol, belonging to the field of preparation of carbon monoxide and ethylene glycol. Under ultraviolet light irradiation, dihydroxyacetone is converted into carbon monoxide and ethylene glycol. The established flow reaction device can achieve the stable flow production of carbon monoxide and ethylene glycol. The present invention is green, economical, and has a high atom utilization rate; the product yield is high; the reaction rate is fast, no catalyst is required, and it is under room temperature and atmospheric pressure conditions, having good application prospects.

The invention relates to the field of fine chemical materials, and realizes a low-cost and high-efficiency preparation method of 1, 3-dihydroxyacetone by taking acetone as a raw material through two steps of halogenation reaction and alkali substitution reaction for the first time. The whole process is simple to operate, green and environment-friendly.

The present invention discloses a method for improving the yield and production intensity of 1,3-dihydroxyacetone by Gluconobacter oxydans, belonging to the technical field of fermentation engineering. In the present invention, dehydrogenase genes that potentially affect the metabolic flux of 1,3-dihydroxyacetone are knocked out in Gluconobacter oxydans to enhance the efficiency of converting its substrate glycerol into 1,3-dihydroxyacetone, thereby improving the yield and production intensity of 1,3-dihydroxyacetone. Compared with the control strain G. oxydans WSH-003, the yields, conversion rates, and production intensities of 1,3-dihydroxyacetone of the recombinant strains G. oxydans WSH-1, G. oxydans WSH-2, G. oxydans WSH-3, G. oxydans WSH-4, G. oxydans WSH-5, G. oxydans WSH-6, G. oxydans WSH-7, and G. oxydans WSH-8 are all significantly improved.

The invention relates to the technical field of glucose synthesis, and provides a method for synthesizing glucose through photo-enzyme cascade catalysis, which comprises the following steps: carrying out photo-catalytic reaction on oxidized coenzyme to obtain reduced coenzyme; taking a carbon dioxide source and a pentose compound as raw materials, performing a first series of enzyme catalytic reactions, and taking the reduced coenzyme as a substrate in the process of the first series of enzyme catalytic reactions to obtain glyceraldehyde-3-phosphoric acid and ketose dihydroxyacetone phosphate; and by taking the glyceraldehyde-3-phosphoric acid and the ketose dihydroxyacetone phosphoric acid as raw materials, carrying out a second series of enzyme catalytic reactions to obtain the glucose. According to the method for synthesizing glucose through photo-enzyme cascade catalysis, two green processes of photo-catalysis and enzyme catalysis are combined, the production period is greatly shortened, the product selectivity and yield are improved, and a new way is opened up for the green synthesis technology of glucose.

The application provides a BFD mutant and application thereof as a carbon chain lengthening enzyme, and comprises: (a) the BFD mutant contains at least one site of L43, A73, T100 and K165 compared with the amino acid sequence shown in SEQ ID NO. 1, and has an amino acid sequence which is subjected to amino acid mutation and has a carbon chain lengthening enzyme activity function; or (b) the BFD mutant has an amino acid sequence which is subjected to substitution, deletion and / or addition of one or more conservative amino acid residues and has the same function. The enzyme activity of the BFD mutant is obviously improved, and the yield and conversion rate of formaldehyde to ethanol aldehyde and / or 1,3-dihydroxyacetone are improved.

The invention relates to the technical field of glass processing, in particular to food-grade cutting fluid for glass processing and a preparation method of the food-grade cutting fluid. The lubricating oil is prepared from the following raw materials in percentage by weight: 50-60% of food-grade base oil, 10-15% of a food-grade lubricant, 3-8% of a food-grade auxiliary stabilizing material and the balance of water, the food-grade auxiliary stabilizing material is composed of a 1, 3-dihydroxy acetone dimer, an emulsion stabilizer, an adsorption stabilizer and an enhancement and protection stabilizer; the emulsion stabilizer is emulsified calcium stearate dispersion liquid and / or sodium polyphosphate; the adsorption stabilizer is a composition of multiple of magnesium trisilicate, food-grade diatomite and food-grade activated clay; the reinforcing and protecting stabilizer is calcium alginate and / or food-grade titanium dioxide, food-grade raw materials are adopted, and the glass cutting agent has a good cutting effect and good quality stability when being used for glass processing.

The present invention relates to a system for producing an in-situ foam comprising one or more poly (amino acids) (A), one or more components (B) capable of reacting with said poly (amino acids) (A) and one or more amphoteric polymers (C) wherein component (B) is selected from the group consisting of reducing sugars, 1, 2, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, and 2, 3-dihydroxyacetone, glycolaldehyde, glyceraldehyde, or any mixture thereof.

The invention discloses a protein fiber dyeing method, a protein fiber and a fabric, according to the protein fiber dyeing method, reducing sugar DHA is reacted with indigo blue, and low-temperature green dyeing and functional modification of the protein fiber are synchronously realized by utilizing the dual functions of a reducing agent and a color developing agent of DHA. Specifically, ketose-dihydroxy acetone (DHA) is used for reducing indigo into a leuco body, meanwhile, the ketose-dihydroxy acetone (DHA) and amino in protein fibers are subjected to a Maillard reaction to generate yellowish-brown pigment, and a unique yellow-green system is formed by combining a blue system of traditional indigo, so that the dyeing chromatography is widened, meanwhile, the dyeing process is green and environmentally friendly, and the dyeing efficiency is improved. The dyed protein fiber is endowed with excellent anti-ultraviolet performance (UPFgt; 1800) and oxidation resistance (the free radical scavenging rate can reach 70%), and the strength of the dyed protein fiber is almost not lost after dyeing.

This invention relates to the field of hydrogen production technology, specifically to a method for producing dihydroxyacetone from glycerol via electrochemical oxidation coupled with hydrogen production. The method includes: S1 preparing a glycerol alkaline solution as the electrolyte solution, the purity of which must reach analytical grade; S2 placing the electrolyte solution prepared in S1 into an electrolytic cell, connecting the positive terminal of a power supply to the anode in the electrolytic cell, and connecting the negative terminal of the power supply to the cathode in the electrolytic cell; S3 turning on the power supply and, at a defined temperature, performing a redox reaction and a hydrogen evolution reaction to obtain dihydroxyacetone prepared by anodic oxidation, and simultaneously obtaining hydrogen gas prepared by cathode hydrogen evolution. This patent constructs a synergistic reaction system of selective anodic oxidation to dihydroxyacetone and efficient cathode hydrogen evolution by using a glycerol alkaline solution as the electrolyte solution. The glycerol alkaline solution weakens the nucleophilic attack intensity of hydroxide ions and inhibits carbon-carbon bond breaking through the in-situ protection mechanism of the glycerol carbon chain, achieving highly selective and directional synthesis of dihydroxyacetone.

The invention provides a carbon chain elongase as well as a coding gene and application thereof, the amino acid sequence of the carbon chain elongase is as shown in a sequence table SEQ ID NO.1, the nucleotide sequence of the gene for coding the carbon chain elongase is as shown in a sequence table SEQ ID NO.2, and the carbon chain elongase can be used as a formaldehyde lyase and has a function of efficiently catalyzing formaldehyde to synthesize 1, 3-dihydroxyacetone.

The application discloses a formaldehyde lyase mutant and application thereof. The formaldehyde lyase mutant provided by the application can regulate the generation proportion of a two-carbon and three-carbon product generated by catalyzing formaldehyde condensation, has improved activity of catalyzing formaldehyde condensation into glycolaldehyde, and reduced activity of catalyzing formaldehyde condensation into 1,3-dihydroxyacetone, compared with formaldehyde lyase.

The invention belongs to the technical field of chemical catalysis, and particularly relates to a supported catalyst, a preparation method of the supported catalyst and application of the supported catalyst to preparation of dihydroxyacetone through glycerol oxidation. The supported catalyst provided by the invention is an Au-based catalyst, the carrier of the supported catalyst is a copper-cerium composite oxide, the gold loading capacity is low, and even if the gold content is low, glycerol oxidation can be efficiently catalyzed in a heterogeneous and non-alkaline environment to prepare DHA. The invention also provides an application of the supported catalyst provided by the scheme or the supported catalyst prepared by the preparation method provided by the scheme in preparation of dihydroxyacetone by glycerol oxidation. The supported catalyst disclosed by the invention is used for preparing DHA through catalytic oxidation under mild reaction conditions by taking glycerol as a raw material, and is small in equipment corrosion and environment-friendly, so that the supported catalyst has wide application potential in the future.

The present invention relates to a binder composition comprising a) component A comprising polymer(s) A1 having primary and / or secondary amino groups, wherein polymer(s) A1 has(have) a primary and secondary amine group nitrogen content of at least 1 wt.-% and b) component B comprising component B1 which is 1,3-dihydroxyacetone, glycolaldehyde, glyceraldehyde or any mixture thereof, wherein the polymer(s) A1 has(have) a total weight average molecular weight Mw,total of at least 800 g / mol.

The invention relates to a preparation method and application of a phosphorus-doped lignin-derived carbon-loaded NiCu alloy material. The preparation method comprises the following steps: preparing phosphorized lignin by phosphorizing a plurality of pairs of lignin, taking the phosphorized lignin as a phosphorus source and a carbon source, regulating pH to enable the phosphorized lignin to be coordinated and combined with Ni and Cu ions to form a nickel / copper-lignin-based supramolecular precursor, and then performing high-temperature carbon burning to obtain the nickel / copper-lignin-based supramolecular composite material. The catalyst combines the advantages of a biomass carbon material and transition metals Cu and Ni, is a catalyst with relatively excellent performance, can be widely applied to electrocatalytic conversion of glycerol, furfural, glucose and other biomass micromolecules into high-value chemicals, and particularly has an excellent effect on conversion of glycerol into a high-value product dihydroxyacetone. The construction of a P-C structure on the carrier is beneficial to the local enrichment of active oxygen groups, the generation of metal active sites is promoted, the oxidation rate of glycerol is accelerated, and NiCu alloying obviously improves the selectivity of dihydroxyacetone. The catalyst is stable in effect, rich in resources and low in price.

The present application discloses a mutant Asaia bogorensis strain (Deposition number DSM 34962) as well as compositions, cell cultures and kits comprising said strain. The present application also discloses the use of said Asaia bogorensis strain, compositions, cell cultures or kits in methods of producing dihydroxyacetone (DHA).

The invention provides a method for detecting the inhibitory activity of non-enzymatic glycosylation end products (AGEs). The method comprises the following steps: preparing a mixed solution containing bovine serum albumin and dihydroxyacetone as a control system; adding a to-be-detected substance into the mixed solution containing bovine serum albumin and dihydroxyacetone to serve as a to-be-detected system; carrying out incubation reaction on the control system and the to-be-detected system at 25-55 DEG C; respectively detecting fluorescence values of the control system and the to-be-detected system; and according to the change of the fluorescence value before and after the to-be-detected substance is added, evaluating whether the to-be-detected substance has an inhibiting effect on the generation of the non-enzymatic glycosylation end product or not. The invention also provides a kit for detecting the inhibitory activity of the non-enzymatic glycosylation end product. According to the detection method and the kit, the incubation reaction time of AGEs is shortened from 7 days to 24 hours, the detection time is greatly shortened, the detection efficiency is improved, and a new choice is provided for high-throughput screening of AGEs inhibitors.

The invention relates to a dihydroxyacetone phosphate transaminase CJ1361 mutant with improved stability, and a gene recombinant bacterium containing the dihydroxyacetone phosphate transaminase CJ1361 mutant and an enzyme liquid of the gene recombinant bacterium as biocatalysts, which are used for synthesizing serinol by a microbial fermentation method. Compared with the wild type dihydroxyacetone phosphate transaminase CJ1361, the yield of serinol produced by the dihydroxyacetone phosphate transaminase CJ1361 mutant provided by the invention is remarkably improved.

The invention discloses a method for preparing medium oxalic acid through biological catalysis, and belongs to the field of biological catalysis. The invention solves the problems of environmental pollution and low process safety caused by the use of a noble metal catalyst and an organic solvent in the conventional oxalic acid production process. According to the method for preparing oxalic acid through biological catalysis, thalli obtained through fermentation culture of recombinant escherichia coli serve as a catalyst, 1, 3-dihydroxyacetone serves as a raw material, 2-ketomalonaldehyde is obtained through catalysis in a water system, then a solution containing oxalic acid is obtained under the catalytic action of succinate semialdehyde dehydrogenase, and the oxalic acid is prepared. And extracting the medium oxalic acid by adopting a solution containing the medium oxalic acid. The preparation method provided by the invention takes the biological enzyme as a catalyst and water as a solvent for reaction, and has the remarkable characteristics of mild reaction conditions, no pollution and the like. The preparation method provided by the invention is suitable for production of medium oxalic acid.

The present invention relates to a process for producing a foam, which comprises foaming a mixture, comprising one or more amino acid copolymers (A), one or more components (B) capable of reacting with said amino acid copolymers (A) and one or more blowing agents (F), wherein component (B) is selected from alpha-hydroxy ketones and alpha-aldehydes such as reducing sugars, 1,3-dihydroxyacetone, glycolaldehyde, glyceraldehyde or any mixture thereof and the foam obtainable by this process.

The invention relates to a polypeptide comprising a bacterial membrane anchoring peptide and an aldolase capable of catalyzing an aldol condensation reaction between two selected compounds to form a sugar. The aldolase may be dependent on dihydroxyacetone phosphate (DHAP) or dihydroxyacetone (DHA). The invention also relates to a bacterium genetically modified to produce sugars, particularly rare sugars, phosphorylated or not, and a method for producing and secreting these sugars by culturing said bacterium in a suitable culture medium M1 comprising two compounds capable of forming a sugar by an aldol condensation reaction.

The invention discloses a method for producing dihydroxyacetone (DHA) through selective photoelectric oxidation electrolysis of glycerol. The method comprises the following steps: preparing a phosphate buffer solution containing H2PO4 <-> and / or HPO4 < 2->; adding glycerol into the phosphate buffer, and uniformly mixing to obtain an electrolyte; and carrying out photoelectric oxidation electrolysis on the electrolyte by adopting a working electrode made of TiO2 so as to obtain the product DHA. The production process is simple and convenient, and is especially suitable for industrial large-scale efficient production of DHA.

The invention belongs to the technical field of chemical catalysis, and particularly relates to a method for preparing dihydroxyacetone by directionally catalyzing and oxidizing glycerol based on a hairpin structure. Comprising the following steps: taking a conductive substrate loaded with a catalyst Ce-coated Fe2O3 as a photo-anode, and assembling the conductive substrate, a cathode and an electrolyte into a photoelectric catalytic reaction device; and adding glycerol and glutaraldehyde into the electrolyte, and selectively oxidizing the glycerol into dihydroxyacetone under the conditions of illumination and voltage application. According to the invention, hydrogen bond acceptor molecule glutaraldehyde with a specific spatial configuration is selected to form a hairpin composite structure with hydroxyl groups at two ends of glycerol, and accurate activation and directional oxidation of intermediate hydroxyl groups are realized under the action of a catalyst Ce-coated Fe2O3, so that glycerol is directionally and efficiently oxidized into 1, 3-dihydroxyacetone, and the yield of 1, 3-dihydroxyacetone is improved. And an innovative solution is provided for high-selectivity conversion of the biomass-derived polyol.

The invention discloses a preparation method and application of high-strength and high-toughness self-repairing polyurethane based on dynamic network reconstruction, and belongs to the technical field of self-repairing materials. The method comprises the following steps: synthesizing a prepolymer from polytetrahydrofuran and isophorone diisocyanate, and carrying out composite chain extension through 1, 3-dihydroxyacetone and 4, 7, 10-trioxo-1, 13-tridecane diamine, so as to form a dynamic hydrogen bond network. Excessive hydrogen bond sites are provided through carbonyl of 1, 3-dihydroxyacetone, dynamic network reconstruction can be carried out under external stress stimulation, and the tensile strength, the ductility and the self-repairing efficiency of the material are synchronously improved. After the dynamic network is reconstructed, fracture and recombination of hydrogen bonds can effectively dissipate strain energy and drive damage repair. The tensile strength of the prepared polyurethane reaches 21.1 MPa, the elongation at break exceeds 1500%, the repair efficiency at 60 DEG C for 12 hours is larger than 90%, and the polyurethane is suitable for the field of flexible intelligent electronic equipment.

This invention relates to the field of plant disease control technology, and in particular to the application of dihydroxyacetone (DHA) in the control of root rot and / or the promotion of plant growth. This invention found that DHA can effectively antagonize the growth of the pathogen *Fusarium graminearum*, with an inhibition rate of 36.14%–48.65%. In soil culture experiments, at 7 days, both high concentrations (1M) and low concentrations (5mM) of DHA showed good control effects on maize root rot, with disease control efficacy reaching 14.17% and 27.08%, respectively. Simultaneously, both showed good growth-promoting effects; low concentrations of DHA increased the aboveground biomass of maize by 65.30% compared to diseased plants. At 14 days, the control efficacy of high concentrations (1M) and low concentrations (5mM) of DHA against maize root rot was 15.24% and 33.05%, respectively, and low concentrations of DHA increased the aboveground biomass of maize by 135.32% compared to diseased plants.