7 results about "Gluconobacter" patented technology

The invention relates to the technical field of bacterial cellulose preparation, in particular to a composite flora for producing bacterial cellulose and a preparation method thereof, and the preparation method comprises the following steps: screening gluconobacter oxydans, obtaining culture medium preparation materials and preparation tools, preparing a slant culture medium set, preparing an enriched liquid culture medium set, and preparing composite flora mixed inoculation liquid. The method comprises the following steps: preparing a waste tobacco leaf leaching solution, extracting a proportional leaching solution from the waste tobacco leaf leaching solution according to a preset proportion, obtaining a mixed fermentation culture medium based on a preparation tool and the proportional leaching solution, and carrying out inoculation operation on a composite flora mixed inoculation solution by using the mixed fermentation culture medium to obtain a dry cellulose membrane; calculating the yield of the bacterial cellulose based on the dry cellulose membrane, and completing the preparation of the composite flora for producing the bacterial cellulose based on the yield of the bacterial cellulose. The method can be used for scientifically, efficiently and stably preparing the composite flora.

The present application relates to the field of microbial technology, and particularly to a strain of Gluconobacter oxydans and its application. Gluconobacter oxydans The present application provides a strain of Gluconobacter oxydans (Gluconobacter oxydans) named KUST4611, which has been preserved in the Guangdong Microbial Culture Collection Center on October 22, 2025, with a preservation number of GDMCC NO: 67143. The volatile substances produced by the strain provided by the present application during growth have a bacteriostatic effect. When the strain is applied to prepare a fresh-keeping gel, the volatile substances produced by the gel placed in a closed space can achieve good fresh-keeping effect, effectively reduce the occurrence of diseases, maintain the freshness of the peppers, and effectively prolong the fresh-keeping period of the peppers. Meanwhile, the preparation method of the fresh-keeping gel is simple, and the fresh-keeping effect can be easily and conveniently achieved, the cumbersome steps of postharvest preservation are reduced, and the postharvest preservation efficiency is further improved.

The application provides a torulopsis pullulans engineering strain and a construction method and application thereof, and belongs to the field of microorganisms and food biotechnology. The application provides a torulopsis pullulans DL-XKX01 engineering strain with high ergothioneine production capacity. The RNA interference technology is used to inhibit the carotenoid synthesis pathway which competes with ergothioneine for substrates, so as to improve the ergothioneine production capacity. The ergothioneine level of the torulopsis pullulans DL-XKX01 provided by the application can reach 116.24+ / -4.33 mg / L in a flask fermentation, and can reach 858.18+ / -5.23 mg / L in a fermenter. The torulopsis pullulans DL-XKX01 can be used to improve the ergothioneine content in fermented food and improve the food quality, and has a good industrial application prospect.

The present disclosure provides a method for culture of bacterial strains, comprising the following steps: inoculating a bacterial strain in a first culture medium comprising 0.1 mg / L-0.8 mg / L of 1-bromohexane to obtain a first culture. The method for culture of bacterial strains provided by the present disclosure further comprises: inoculating the first culture into a second culture medium, wherein the second culture medium comprises 0.2 mg / L-0.8 mg / L of 1-bromohexane. The method can be used for fermentation of Gluconacetobacter melanogenum, Propionibacterium, Corynebacterium, Brevibacterium, Bacillus, Gluconobacter, Lactobacillus, Agromyces, Actinobacillus, Escherichia coli, Anaerobiospirillum, Trichoderma, Streptomyces, Aspergillus, Saccharomyces or Myceliopthora, effectively preventing infection of bacteriophages during fermentation.

The invention belongs to the technical field of microorganisms, and particularly relates to gluconobacter oxydans and application thereof. The strain ZLV2-2 is classified and named as Gluconobacter oxydans, the Gluconobacter oxydans is preserved in China General Microbiological Culture Collection Center (CGMCC) on October 29, 2025, and the preservation number is CGMCC NO.36411. The strain ZLV2-2 is named as Gluconobacter oxydans. The Gluconobacter oxydans ZLV2-2 provided by the invention has strong genetic stability, can efficiently ferment and convert isopentenol to synthesize isopentenoic acid, has a wide action substrate spectrum, also has a good conversion effect on primary alcohols such as n-propanol and n-butanol, and has industrial application potential; the method is simple in production process, mild in condition, green and safe, avoids the problems of high energy consumption and environmental pollution of a traditional chemical method, has few by-products, and is beneficial to extraction and separation operation.

The present invention has an object to provide a D-mannose isomerase of membrane-bound form having the optimal reaction pH in acidic region. The membrane-bound D-mannose isomerase derived from acetic acid bacteria is produced. The acetic acid bacteria preferably belong to the genera of Acetobacter, Gluconobacter, or Gluconacetobacter. Furthermore, the membrane-bound D-mannose isomerase from the acetic acid bacteria is used to produce D-fructose from D-mannose.

The application discloses a method for improving the anti-styrene stress ability of Gluconobacter oxydans, and belongs to the technical field of genetic engineering and microbial engineering. Firstly, the adaptability evolution of wild-type Gluconobacter oxydans to styrene is carried out by gradient addition of styrene in a culture medium, and finally, the evolved strain ST significantly improves the tolerance of Gluconobacter oxydans to styrene. Secondly, the knocking out of flagellin FlgE in Gluconobacter oxydans also improves the stress ability of Gluconobacter oxydans to styrene; the growth performance of the gene deletion Gluconobacter oxydans ST-ΔflgE prepared by the method of the application after 10h culture under 15g / L styrene is far better than that of the wild-type Gluconobacter oxydans.