18 results about "Gluconobacter" patented technology

The present invention relates to a strain of Glutamicibacter endophyticus subsp. halotolerans. The strain Glutamicibacter endophyticus NY3-1 of Glutamicibacter endophyticus subsp. halotolerans is deposited in the China Center for Type Culture Collection with the deposit number CCTCC No: M20242321. The 16S rDNA nucleotide sequence of the strain Glutamicibacter endophyticus NY3-1 is shown as SEQ ID NO: 1. This strain can metabolize normally in both salt-free and salt-containing environments, and can tolerate environments with a pH value of 6-11. It has the effects of improving the germination rate of plant seeds and promoting plant growth in both salt-containing and salt-free environments. Moreover, this strain can improve the soil environment of saline-alkali land, has broad application prospects, and provides a new biological resource for the biological improvement of saline-alkali land.

The invention relates to the technical field of bacterial cellulose preparation, in particular to a composite flora for producing bacterial cellulose and a preparation method thereof, and the preparation method comprises the following steps: screening gluconobacter oxydans, obtaining culture medium preparation materials and preparation tools, preparing a slant culture medium set, preparing an enriched liquid culture medium set, and preparing composite flora mixed inoculation liquid. The method comprises the following steps: preparing a waste tobacco leaf leaching solution, extracting a proportional leaching solution from the waste tobacco leaf leaching solution according to a preset proportion, obtaining a mixed fermentation culture medium based on a preparation tool and the proportional leaching solution, and carrying out inoculation operation on a composite flora mixed inoculation solution by using the mixed fermentation culture medium to obtain a dry cellulose membrane; calculating the yield of the bacterial cellulose based on the dry cellulose membrane, and completing the preparation of the composite flora for producing the bacterial cellulose based on the yield of the bacterial cellulose. The method can be used for scientifically, efficiently and stably preparing the composite flora.

The invention belongs to the technical field of gene engineering, and discloses a promoter and application thereof in enhancing target gene expression and promoting mannitol metabolism to synthesize bacterial cellulose. The nucleotide sequence of the promoter P00300 is as shown in SEQ ID NO: 2. The promoter is obtained by site-directed mutagenesis of a wild type promoter P06915 of gluconacetobacter xylinum and screening. The promoter provided by the invention can be used for enhancing the expression of a target gene and producing a target compound. For example, the expression level of fk, mdh1 and mdh2 three-gene combination is remarkably improved, the recombinant strain can efficiently synthesize BC by using mannitol, and the yield reaches 6.1 g / L, is about 2.87 times of that of a wild strain, and is 1.27 times of that of a bla promoter in pBla. The result lays a foundation for further understanding mannitol metabolism of gluconacetobacter xylinum and for high-value conversion and utilization of marine carbon resource mannitol and BC synthesis.

The present invention discloses the construction and application of a recombinant Gluconobacter oxydans, belonging to the fields of genetic engineering and whole-cell catalysis. The present invention heterologously expresses an epoxide hydrolase from Sphingomonas in Gluconobacter oxydans, and simultaneously utilizes the alcohol and aldehyde dehydrogenases bound to the membrane of Gluconobacter oxydans itself to synthesize R-mandelic acid in a one-step process using styrene oxide as a substrate. The present invention combines the transcriptome data of Gluconobacter oxydans, screens 7 strong promoters using green fluorescent protein as a reporter gene, and determines the strongest promoter P for expressing the epoxide hydrolase gene. 12780 , ultimately significantly increasing the yield of R-mandelic acid.

The present invention discloses a method for improving the yield and production intensity of 1,3-dihydroxyacetone by Gluconobacter oxydans, belonging to the technical field of fermentation engineering. In the present invention, dehydrogenase genes that potentially affect the metabolic flux of 1,3-dihydroxyacetone are knocked out in Gluconobacter oxydans to enhance the efficiency of converting its substrate glycerol into 1,3-dihydroxyacetone, thereby improving the yield and production intensity of 1,3-dihydroxyacetone. Compared with the control strain G. oxydans WSH-003, the yields, conversion rates, and production intensities of 1,3-dihydroxyacetone of the recombinant strains G. oxydans WSH-1, G. oxydans WSH-2, G. oxydans WSH-3, G. oxydans WSH-4, G. oxydans WSH-5, G. oxydans WSH-6, G. oxydans WSH-7, and G. oxydans WSH-8 are all significantly improved.

The present application relates to the field of microbial technology, and particularly to a strain of Gluconobacter oxydans and its application. Gluconobacter oxydans The present application provides a strain of Gluconobacter oxydans (Gluconobacter oxydans) named KUST4611, which has been preserved in the Guangdong Microbial Culture Collection Center on October 22, 2025, with a preservation number of GDMCC NO: 67143. The volatile substances produced by the strain provided by the present application during growth have a bacteriostatic effect. When the strain is applied to prepare a fresh-keeping gel, the volatile substances produced by the gel placed in a closed space can achieve good fresh-keeping effect, effectively reduce the occurrence of diseases, maintain the freshness of the peppers, and effectively prolong the fresh-keeping period of the peppers. Meanwhile, the preparation method of the fresh-keeping gel is simple, and the fresh-keeping effect can be easily and conveniently achieved, the cumbersome steps of postharvest preservation are reduced, and the postharvest preservation efficiency is further improved.

The application provides a torulopsis pullulans engineering strain and a construction method and application thereof, and belongs to the field of microorganisms and food biotechnology. The application provides a torulopsis pullulans DL-XKX01 engineering strain with high ergothioneine production capacity. The RNA interference technology is used to inhibit the carotenoid synthesis pathway which competes with ergothioneine for substrates, so as to improve the ergothioneine production capacity. The ergothioneine level of the torulopsis pullulans DL-XKX01 provided by the application can reach 116.24+ / -4.33 mg / L in a flask fermentation, and can reach 858.18+ / -5.23 mg / L in a fermenter. The torulopsis pullulans DL-XKX01 can be used to improve the ergothioneine content in fermented food and improve the food quality, and has a good industrial application prospect.

The present invention discloses a method for improving the yield and production intensity of 1,3-dihydroxyacetone by Gluconobacter oxydans, belonging to the technical field of fermentation engineering. By knocking out dehydrogenase genes in Gluconobacter oxydans that potentially affect the metabolic flux of 1,3-dihydroxyacetone, the efficiency of converting its substrate glycerol into 1,3-dihydroxyacetone is enhanced, thereby improving the yield and production intensity of 1,3-dihydroxyacetone. Compared with the control strain G. oxydans WSH-003, the yields, conversion rates and production intensities of 1,3-dihydroxyacetone of the recombinant strains G. oxydans WSH-1, G. oxydans WSH-2, G. oxydans WSH-3, G. oxydans WSH-4, G. oxydans WSH-5, G. oxydans WSH-6, G. oxydans WSH-7 and G. oxydans WSH-8 are all significantly improved.

The invention discloses a LysR type transcriptional regulatory factor knockout Gluconobacter sp. And application thereof in continuous catalysis of DHA production, and belongs to the technical field of bioengineering. In order to improve the yield of DHA (1, 3-dihydroxyacetone), the expression levels of genes under different fermentation processes of batch fermentation and continuous catalytic perfusion are analyzed and compared through transcriptome, the relationship between the gene expression and the yield of 1, 3-dihydroxyacetone is explored, differential genes in the relationship are analyzed and verified, and it is found that after a LysR type transcriptional regulation factor is knocked out, the expression level of the 1, 3-dihydroxyacetone is analyzed and verified. And the yield of the 2, 3-dihydroxyacetone is obviously improved. After 12 days of continuous catalytic perfusion fermentation production, the cumulative yield of 1, 3-dihydroxyacetone is improved by 41.92% compared with the cumulative yield of 1, 3-dihydroxyacetone of a control strain, and the glycerin conversion rate is improved by 12.58% compared with the glycerin conversion rate of the control strain. Metabolism of Gluconobacter is changed by knocking out a LysR type transcriptional regulation factor, so that synthesis of 1, 3-dihydroxyacetone is influenced.

The present disclosure provides a method for culture of bacterial strains, comprising the following steps: inoculating a bacterial strain in a first culture medium comprising 0.1 mg / L-0.8 mg / L of 1-bromohexane to obtain a first culture. The method for culture of bacterial strains provided by the present disclosure further comprises: inoculating the first culture into a second culture medium, wherein the second culture medium comprises 0.2 mg / L-0.8 mg / L of 1-bromohexane. The method can be used for fermentation of Gluconacetobacter melanogenum, Propionibacterium, Corynebacterium, Brevibacterium, Bacillus, Gluconobacter, Lactobacillus, Agromyces, Actinobacillus, Escherichia coli, Anaerobiospirillum, Trichoderma, Streptomyces, Aspergillus, Saccharomyces or Myceliopthora, effectively preventing infection of bacteriophages during fermentation.

The present invention discloses a method for improving 1,3-dihydroxyacetone yield and production intensity of Gluconobacter oxydans, and belongs to the field of fermentation engineering technology. The present invention enhances the efficiency of converting its substrate glycerol into 1,3-dihydroxyacetone by knocking out the dehydrogenase gene that has a potential impact on the metabolic flux of 1,3-dihydroxyacetone in Gluconobacter oxydans, thereby improving the yield and production intensity of 1,3-dihydroxyacetone. Compared with the control strain G.oxydansWSH-003, the 1,3-dihydroxyacetone yield, conversion rate and production intensity of recombinant strains G.oxydans WSH-1, G.oxydans WSH-2, G.oxydans WSH-3, G.oxydans WSH-4, G.oxydans WSH-5, G.oxydans WSH-6, G.oxydans WSH-7 and G.oxydans WSH-8 are significantly improved.

The invention belongs to the technical field of microorganisms, and particularly relates to gluconobacter oxydans and application thereof. The strain ZLV2-2 is classified and named as Gluconobacter oxydans, the Gluconobacter oxydans is preserved in China General Microbiological Culture Collection Center (CGMCC) on October 29, 2025, and the preservation number is CGMCC NO.36411. The strain ZLV2-2 is named as Gluconobacter oxydans. The Gluconobacter oxydans ZLV2-2 provided by the invention has strong genetic stability, can efficiently ferment and convert isopentenol to synthesize isopentenoic acid, has a wide action substrate spectrum, also has a good conversion effect on primary alcohols such as n-propanol and n-butanol, and has industrial application potential; the method is simple in production process, mild in condition, green and safe, avoids the problems of high energy consumption and environmental pollution of a traditional chemical method, has few by-products, and is beneficial to extraction and separation operation.

The invention discloses a method for improving acid production of cells through cooperation of diatom and gluconobacter oxydans, and belongs to the technical field of bioengineering. The diatom and the gluconobacter oxydans are simultaneously inoculated into a sugar-containing liquid culture medium and are aerated and stirred, and then fermentation is performed. According to the method, the gluconic acid or the xylonic acid is efficiently prepared through biotransformation of the diatom and the gluconobacter oxydans, the yield of the gluconic acid or the xylonic acid added with the diatom under the same condition is obviously higher than that of the gluconic acid or the xylonic acid not added with the diatom in each time period, efficient acid production of cells is achieved, and the method has good innovativeness and practical value.

The application discloses a method for improving the tolerance of Gluconobacter oxydans to phenylethylene stress by overexpressing a gene marR and belongs to the technical field of genetic engineering and microbial engineering. The application significantly improves the tolerance of Gluconobacter oxydans to phenylethylene stress by overexpressing a MarR family transcriptional regulator marR (GOX_RS11840) in Gluconobacter oxydans. The survival rate of the recombinant Gluconobacter oxydans prepared by the method of the application after stress culture for 10 hours in an environment with 15 g / L phenylethylene is 1.6 times that of a control strain of Gluconobacter oxydans.

The present invention has an object to provide a D-mannose isomerase of membrane-bound form having the optimal reaction pH in acidic region. The membrane-bound D-mannose isomerase derived from acetic acid bacteria is produced. The acetic acid bacteria preferably belong to the genera of Acetobacter, Gluconobacter, or Gluconacetobacter. Furthermore, the membrane-bound D-mannose isomerase from the acetic acid bacteria is used to produce D-fructose from D-mannose.

The application discloses a method for improving the anti-styrene stress ability of Gluconobacter oxydans, and belongs to the technical field of genetic engineering and microbial engineering. Firstly, the adaptability evolution of wild-type Gluconobacter oxydans to styrene is carried out by gradient addition of styrene in a culture medium, and finally, the evolved strain ST significantly improves the tolerance of Gluconobacter oxydans to styrene. Secondly, the knocking out of flagellin FlgE in Gluconobacter oxydans also improves the stress ability of Gluconobacter oxydans to styrene; the growth performance of the gene deletion Gluconobacter oxydans ST-ΔflgE prepared by the method of the application after 10h culture under 15g / L styrene is far better than that of the wild-type Gluconobacter oxydans.

The invention discloses a method for regulating and controlling the component proportion of a bacterial cellulose / hyaluronic acid composite membrane on the basis of genetic engineering strain combination mixed fermentation, and belongs to the technical field of biological materials. The bacterial strain combination comprises at least two genetically modified gluconacetobacter xylinus engineering bacterial strains, and the engineering bacterial strains comprise a wild type bacterial strain for expressing empty plasmids, a bacterial strain for expressing HA recombinant plasmids, and a mutant bacterial strain for knocking out bcsH / Y / Z genes and expressing the HA recombinant plasmids. The composite membrane is obtained by regulating and controlling the inoculation ratio (1: 0.1-10) of different strains and carrying out static culture and post-treatment in an HS culture medium containing chloramphenicol. According to the method, regulation and control of the mass ratio (0-7.5%) of BC to HA in the composite membrane are innovatively realized; when the proportion of an HA expression strain is increased, the content of HA is adjustable between 0% and 1.3%, and the proportion of HA can be increased to 3% by adopting a bcs gene knockout strain combination. The obtained composite film has tunable physicochemical properties, and has important application value in the field of biomedical materials.

The application discloses a gluconobacter oxydans for leaching waste printed circuit boards and a leaching method thereof, and belongs to the technical field of microbial resource recycling and treatment, wherein a leaching solution for leaching and extraction comprises the gluconobacter oxydans, metabolites of the gluconobacter oxydans and an H2O2 solution, and the percentage of metal leaching and the leaching time are shortened by adding the H2O2 solution. Compared with traditional chemical and biological leaching, the application realizes efficient leaching of copper in the waste printed circuit boards under relatively mild conditions, is more safe, and can reduce secondary pollution to the environment.