63 results about "Arabitol" patented technology

The invention discloses a method for producing xylitol and arabitol simultaneously by utilizing xylose mother liquid, which comprises the following steps: (1) diluting the xylose mother liquid, and obtaining a purifying liquid after decolorizing filtration and ion exchange; (2) adding nutrients into the purifying liquid, sterilizing, and then inoculating yeast and fermenting; (3) filtering and sterilizing the fermenting liquid, then carrying out the decolorizing filtration and the ion exchange, and carrying out initial concentration; (4) allowing the concentrated liquid to be prepared into mixed sugar alcohol by catalytic hydrogenation; (5) carrying out chromatographic separation for the mixed sugar alcohol after the decolorizing filtration and the ion exchange to obtain two fractions which are respectively a xylitol fraction and an arabitol fraction; and (6) carrying out evaporation concentration and crystallization for the two fractions so as to obtain xylitol crystals and arabitol crystals. The invention converts xylose and arabinose into xylitol and arabitol by hydrogenation, the conversion rate is higher compared with the method for producing xylitol by yeast fermentation.

The invention relates to a novel admixture for mineral binders, composed of a composition containing at the same time a sugar or an oxidized sugar and a hydrogenated sugar. The oxidized sugar may consist, in particular, of gluconic acid or one of its salts or an oxidized starch hydrolysate. The hydrogenated sugar may consist in particular of maltitol, sorbitol, mannitol, xylitol, arabitol, or a hydrogenated starch hydrolysate containing at least 40% by weight of maltitol. Within the admixture, this composition may be combined with conventional admixtures such as phosphates, borates, or amines. The combination of a sugar or an oxidized sugar and a hydrogenated sugar within this composition makes it possible to obtain synergistic effects, particularly in terms of plasticity and mechanical properties of the mineral binders. The admixtures claimed may be used equally well for admixing with cements, raw materials for cements as for admixing with mortars, slurries and concretes. They may also be used in other sectors such as, for example, the plaster industry, particularly as complexing agents or hydration or dehydration controllers.

The invention relates to a method for detecting alkaloid in a plant extract or a preparation. The alkaloid optionally comprises one or more of fagopyrin and 1, 4-dideoxy-1, 4-imino group -D-arabitol.The method comprises the following steps: carrying out a derivatization reaction on a to-be-detected plant extract or the preparation by using a derivatization reagent to obtain a derivatization product containing an alkaloid derivative; and (2) carrying out chromatographic analysis on the derivatization product obtained in the step (1), wherein the temperature of a chromatographic column is 5+ / -2-20+ / -2 DEG C. According to the method, multiple alkaloid components with similar structures can be detected at the same time, and production monitoring and content determination of the related preparations are facilitated.

The invention discloses a technique for producing xylitol and dulcitol simultaneously, comprising the following steps: (1) diluting xylose mother liquor, carrying out chromatographic separation after decoloration, filtering and ion exchange and obtaining mixed liquor; (2) preparing the xylitol: adding nutritional components into the mixed liquor, inoculating yeast seed liquor after sterilization, carrying out fermentation and obtaining fermentation liquor; (3) filtering the fermentation liquor to remove the yeast, then carrying out decoloration, filtering and ion exchange, and then obtaining crystalline xylitol by evaporation, concentration and crystallization; (4) carrying out evaporation and concentration on the mixed liquor of galactose and arabitol obtained by chromatographic separation in the step (1), concentrating to the mass concentration of 35 to 45 percent, and then carrying out catalytic hydrogenation, thus obtaining the mixed liquor of the dulcitol and the arabitol; and (5) carrying out decoloration, filtering and ion exchange on the obtained mixed liquor, putting the mixed liquor into a crystallization kettle for evaporation till supersaturation, adding dulcitol seed crystal and seed crystal growth promoter, and carrying out crystallization to obtain crystalline dulcitol.

The invention discloses a Gluconobacter oxydans, which is classified and named as Gluconobacter oxydans NH-10, and preserved in China General Microbiological Culture Center of Microbial Culture Collection Management Committee with the preservation number of CGMCC No.2709. The invention further discloses a D-xylulose preparation method that uses the Gluconobacter oxydans. The strain obtained by screening can transform D-Arabitol so as to generate D-xylulose in high efficiency, the D-xylulose preparation method reaches the highest D-Arabitol transformation ratio of 99.5 percent (w / w) and the D-xylulose yield ratio of 95 percent, the D-xylulose concentration of a conversion fluid can reach 95g / L, and the conversion fluid hardly contains other ingredients.

The present invention provides methods for producing recombinant peptides in a bacterial host utilizing a mannitol, arabitol, glucitol, or glycerol-inducible promoter, wherein the host bacterial cell that produces the peptide has been rendered incapable of degrading or metabolizing mannitol, arabitol, or glucitol, or derivatives or analogues thereof. The present invention provides bacterial cells that have been genetically altered to inhibit the metabolism or degradation of mannitol, glucitol, or arabitol, or derivatives or analogues thereof. The present invention utilizes mannitol, arabitol, glucitol, or glycerol to induce expression of a target polypeptide from an inducible promoter, allowing for the use of an inexpensive and stable carbon source inducer in the fermentation processes for the production of recombinant peptides.

Disclosed herein are Xylitol dehydrogenase-inactivated and arabinose reductase-inhibited mutant of Candida tropicalis, a method of producing a high yield of xylitol using the same, and xylitol produced by the method. More specifically, disclosed are a method for producing a high yield of xylitol, in which a high concentration of xylose contained in a biomass hydrolyzate is converted to xylitol using xylitol dehydrogenase-inactivated mutant of Candida tropicalis, without controlling dissolved oxygen to a low level, as well as xylitol produced according to the method. Also disclosed are a xylitol production method, in which the production of byproduct arabitol, which is produced when using a biomass as a substrate and adversely affects the yield of xylitol, is significantly reduced through the use of Candida tropicalis mutant ara-89 (KCTC 11136bp) having an inhibited activity of arabinose reductase converting arabinose to arabitol, thus increasing xylitol productivity, as well as xylitol produced by the method.

A xylitol sweet food formula and a production method thereof belong to the technical field of sweet foods. The formula comprises the following raw materials by weight: 60-65g of xylitol, 25-30g of maltitol, 2-4g of arabitol and 1-3g of sorbitol. The production method of the xylitol sweet food comprises the following process steps: materials are put into a dissolving tank for dissolution, and filtration is carried out after the dissolution; filtrate is stored, and then the filtrate is evaporated; vacuum crystallization is carried out on evaporated liquor, and crystallization at atmospheric pressure is further carried out after vacuum crystallization; crystals are dried, the crystals after drying are screened and magnetized, and then packaged. The invention is the sugar-free nutrition-enriched sweet food formula which takes the xylitol as the main raw material, takes the maltitol, the arabitol, the sorbitol and other non-sugar substances as the auxiliary materials and appropriately adds iron, zinc, calcium, magnesium, vitamins and other substances. The invention avoids the limitations of using xylitol, and the like as the single non-sugar sweetener and greatly strengthens the functions thereof.

The invention relates to a ruthenium and nickel / activated carbon co-loaded catalyst, a preparation method thereof and application of the ruthenium and nickel / activated carbon co-loaded catalyst to preparation of sugar alcohols by sugar through catalytic hydrogenation. Through a simple impregnation reduction method, metal ruthenium and nickel are co-loaded to activated carbon with the high adsorption speed and large specific surface area to prepare the Ru-Ni / AC catalyst, and the Ru-Ni / AC catalyst is applied to preparation of the series of sugar alcohols such as xylitol, arabitol, sorbitol, mannose and maltol. The catalyst is high in activity, good in stability, capable of being reused in multiple batches, and high in product yield. In a word, the catalyst is simple in preparation process, low in production cost, high in catalytic efficiency, good in stability, suitable for the preparation process of the multiple sugar alcohols, and good in universality.

The invention relates to xylitol genetic engineering bacteria and a method for producing xylitol via mixed transformation by the same, and relates to the field of biology thechnology. The invention provides the construction of xylitol genetic engineering bacteria E.coliBL21-xdh06. The genetic engineering bacteria are obtained by acquiring xdh genes of xylitoldehydrogenase (XDH) from gluconobacteroxydans via a PCR method and cloning the xdh genes performing stable and efficient expression in host bacteria E.coliBL21. The xylitoldehydrogenase expressed by the genetic engineering bacteria is increased by 10 times in enzyme activity; and genetic engineering bacteria of D-arabitol is increased from 29% to 91.6% via mixed static transformation of the D-arabitol by using the genetic engineering bacteria and gluconobacteroxydans to produce the xylitol.

The invention relates to a method for preparing xylitol by a complex enzyme method, the obtained xylitol and the application of the xylitol. The method is as follows: arabitol oxidizing dehydrogenase, xylitol reductase and inductive agent xylitol are added into d-arabitol, calcium carbonate and sugar so as to prepare the xylitol. The method has strong continuous operability, low equipment investment and operation cost, easy operation and industrialization realization and very high conversion rate and production rate of the xylitol. The xylitol prepared by the method can be used for tobacco products.

The invention relates to a method for separating and extracting L-ribose from a reaction liquid in which L-Arabitol is used for synthesizing the L-ribose, comprising the following steps of centrifuging the reaction liquid, adopting ion-exchange chromatography and moving bed (SMB) chromatography, concentrating and crystallizing to obtain the L-ribose. The purity of the L-ribose is more than 95% after crystallization. The invention has the characteristics of simple operation, high product purity, light color, high yield and the like.

The present invention provides methods for producing recombinant peptides in a bacterial host utilizing a mannitol, arabitol, glucitol, or glycerol-inducible promoter, wherein the host bacterial cell that produces the peptide has been rendered incapable of degrading or metabolizing mannitol, arabitol, or glucitol, or derivatives or analogues thereof. The present invention provides bacterial cells that have been genetically altered to inhibit the metabolism or degradation of mannitol, glucitol, or arabitol, or derivatives or analogues thereof. The present invention utilizes mannitol, arabitol, glucitol, or glycerol to induce expression of a target polypeptide from an inducible promoter, allowing for the use of an inexpensive and stable carbon source inducer in the fermentation processes for the production of recombinant peptides.

The invention relates to an antibacterial and anti-ultraviolet microcapsule finishing agent and a preparation method thereof. The preparation method comprises the following steps: (1) adding red nan leaf essential oil, juniper oil, and 4-tert-butylphenyl salicylate , chitosan, polyethylene glycol, gardenia yellow, Xanthania sorbifolium oil, fatty alcohol polyoxyethylene ether, betulinic acid, arabitol, lignocellulose and isopropanol are mixed, and ball milled in a ball mill; (2 ) Take out the grinding mixture and add xanthan gum and water to stir; (3) Mix and stir gelatin and water, filter to remove impurities; (4) Add gelatin solution to the mixture in (2), and stir after ultrasonic oscillation; ( 5) Add acetic acid and continue to stir; (6) Add the remaining water to react and cool to below 10°C; (7) Add the remaining components, let it stand for solidification, and wash with water until it becomes odorless and neutral. The prepared finishing agent is naturally harmless to human body, has good protection function to ultraviolet rays, and has good antibacterial effect at the same time.

The invention relates to a method for catalyzing sugar and sugar alcohol hydrocracking reaction through a nickel-based catalyst. Under the action of the nickel-based catalyst, the aqueous solution of xylose, glucose, fructose, arabinose, xylitol, sorbitol, arabitol and other carbon-rich sugar and sugar alcohol is adopted as the raw material, the raw material is subjected to the hydrogenation dehydration and decomposed into low-carbon alcohols in a highly selective manner, and the low-carbon alcohols mainly comprise ethanediol, 1,2-propylene glycol and 1,3-propylene glycol.

The invention discloses a method for simultaneously detecting 22 sugars, sugar alcohol and alcohol in fruit juice, and belongs to the field of analytical chemistry. The method adopts an integral impulse ampere-ion chromatography to research experiment factors affecting component separation via a processing method before optimization; and the method for simultaneously detecting the 22 sugars, the sugar alcohol and the alcohol (2,3-butanediol, propylene glycol, methyl alcohol, glycerin, erythritol, xylitol, rhamnose, arabitol, sorbitol, trehalose, galactitol, mannitol, 2-deoxidized-D-glucose, arabinose, melibiose, glucose, galactose, fructose, ribose, saccharose, raffinose and maltose) at a time is built. The technical difficulty of accurately analyzing the plurality of components, sugars, sugar alcohol and alcohol, in the fruit juice is solved; the method is fast, accurate and sensitive, achieves simultaneous analysis of the plurality of categories and the plurality of components, and makes an important contribution to component detection of the fruit juice.

The invention relates to a method for preparing arabitol by flammulina velutipes, which utilizes the characteristic that flammulina velutipes sporocarp contains rich arabitol to prepare the low-cost and environment-friendly arabitol by taking the flammulina velutipes sporocarp as a raw material; and the product can be taken as natural sweetener and functional food raw material. The method comprises the steps of: drying the fresh flammulina velutipes sporocarp, and then using high-concentration alcohol to soak or carry out reflux extraction on the dried flammulina velutipes sporocarp; carrying out vacuum concentration on the leach liquor to recover ethanol; putting the concentrated solution in the environment of 0-10 DEG C; separating out the arabitol by crystallization; filtering or centrifuging while the arabitol is cool to obtain arabitol crystal; then, carrying out recrystallization on the arabitol crystal by methanol; continuously carrying out vacuum concentration on the separated mother liquor of the crystal to recover ethanol; and repeating the operation of crystallization and recrystallization to improve the yield of the arabitol. In order to save the cost, the method is more suitable for taking defective flammulina velutipes sporocarp or the head cut from the flammulina velutipes sporocarp as raw material. The technology has the characteristics of being simple in equipment, saving the cost and protecting the environment, thus improving the economic benefit of the flammulina velutipes industry.

Novel compounds, called liamocins from Aureobasidium pullulans, having the general structure in Formula 1 are disclosed.where R1 is either COCH3 or H; and R2 is between two to ten O-linked 3,5-dihydroxydecanoate; and R3 can be a polyol (e.g., L- or D-glycerol, L- or D-threitol, L- or D-erythritol, L- or D-arabitol, L- or D-xylitol, L- or D-lyxitol, L- or D-ribitol, L- or D-allitol, L- or D-altritol, L- or D-mannitol, L- or D-iditol, L- or D-gulitol, L- or D-glucitol (also called sorbitol), L- or D-galactitol (also called dulcitol), and L- or D-talitol), 2-amino-D-mannitol, 2N-acetylamino-D-mannitol, L-rhamnitol, or D-fucitol; except when R3 is D-mannitol, R2 is not 2 nor 3 O-linked 3,5-dihydroxydecanoate chains. These liamocins described above in addition to D-mannitol liamocin A1, D-mannitol liamocin A2, D-mannitol liamocin B1, and D-mannitol liamocin B2, alone or in combination with each other, can be used to kill certain bacteria and to treat certain bacterial infections.

The present invention relates to an efficient production method of xylitol by using the xylitol producing microorganism introduced with arabinose metabolic pathway to inhibit the production of arabitol, the byproduct, and instead to use arabinose only for cell metabolism in xylose / arabinose mixed medium. More precisely, to express efficiently L-arabinose isomerase (araA), L-ribulokinase (araB) and L-ribulose-5-phosphate 4-epimerase (araD) in Candida tropicalis, codon optimization was performed. Then, each gene was inserted in the gene expression cassette containing the glyceraldehyde-3-phosphate dehydrogenase promoter and the selection marker URA3, which was introduced into Candida sp. microorganism. As a result, arabitol, the byproduct interrupting the purification and crystallization of xylitol could be inhibited, making the production method of xylitol of the present invention more efficient. The xylitol producing microorganism introduced with arabinose metabolic pathway of the present invention can be effectively used for the production of xylitol with high productivity by inhibiting the generation of arabitol.

The invention discloses a method for preparing D-xylosone by microbial transformation of glucose, comprising the following steps: firstly, respectively preparing an osmophilic yeast seed liquid and a gluconobacter oxydans seed liquid by using glucose as a raw material, then inoculating the osmophilic yeast seed liquid to conduct arabitol fermentation, and then in middle and later stage inoculating the gluconobacter oxydans seed liquid to conduct mixed culture fermentation, simultaneously controlling the glucose content in the broth to be 5-10g / L, and converting arabitol into D-xylosone. According to the method, the feedback inhibition of arabitol is removed, and the efficiency of preparing D-xylosone from glucose is raised. The invention further discloses a method for preparing xylitol by microbial transformation of glucose, comprising the following steps: firstly converting glucose into D-xylosone by the above method, then converting D-xylosone into D-xylose by isomerization of enzyme, extracting and refining, and conducting catalytic hydrogenation to obtain xylitol. According to the method, the production efficiency of xylitol is raised, and the cost of preparing xylitol by biological method is reduced.

The invention provides dry compositions for preparing and loading a sample on a gel for electrophoretic separation. The dry compositions preferably include a tracking dye and a sedimenting agent selected from a five-carbon polyol (e.g., ribitol, arabitol, or xylitol), iso-erythritol, maltitol, and saccharine. Methods for making and using, as well as kits comprising the disclosed compositions, are also provided.

The invention discloses a preparation method of arabitol. The method comprises the steps as follows: performing a nanofiltration operation of 500-molecular weight on an edible fungus extracting solution; collecting a fraction with the molecular weight less than 500; performing a reverse osmosis membrane filtration operation on the extracting solution with the molecular weight less than 500; concentrating feed liquid until the soluble solid content reaches 40-50%, adding absolute ethyl alcohol for crystallization, and performing suction filtration to obtain crude crystals; mixing the crude crystals with absolute ethyl alcohol at the weight ratio of 1:20 and heating until the mixture is dissolved, standing still, naturally cooling to be recrystallized, performing suction filtered, and dryingto obtain high-purity sugar alcohol crystals. The preparation method of the arabitol provided by the invention is simple to operate, and the prepared arabitol is high in purity.

The invention relates to a multi-walled carbon nanotube loaded ruthenium catalyst and a preparation method thereof, and an application of the multi-walled carbon nanotube loaded ruthenium catalyst inpreparation of sugar alcohol by catalytic hydrogenation of sugar. According to the invention, a Ru / MWCNTs catalyst with high dispersity is prepared by loading metal ruthenium (Ru) onto multi-walled carbon nanotubes (MWCNTs) through simple liquid-phase reduction and wet impregnation processes. Furthermore, the multi-walled carbon nanotube loaded ruthenium catalyst is applied to a hydrogenation reaction of a series of sugars like xylose, arabinose, glucose, mannose and maltose to respectively prepare functional sugar alcohols like xylitol, arabitol, sorbitol, mannitol and maltitol. The catalystprovided by the invention can realize high-efficiency conversion of a series of sugars and high-selective preparation of sugar alcohols under the conditions of high substrate sugar concentration and small catalyst usage amount; meanwhile, the catalyst has good stability and high repeated use times. In summary, the catalyst provided by the invention has the advantages of simple preparation process,high active metal dispersity, good catalytic activity, applicability to hydrogenation of various sugars, and good versatility.

Photoresist strippers and cleaning compositions of this invention are provided by non-aqueous, non-corrosive cleaning compositions that resist galvanic corrosion when used on stacked layer structures of different types of metals at a surface of an electronic device. Such non-aqueous photoresist strippers and cleaning compositions comprise: (a) at least one polar organic solvent, (b) at least one di or polyamine having both at least one primary amine group and one or more secondary and / or tertiary amine groups, and having the formula wherein R1, R2, R4, and R5 can be independently selected from H, OH, hydroxyalkyl and aminoalkyl groups; R6 and R7 are each independently H or alkyl groups, and m and n are each independently integers of 1 or larger, with the proviso that R1, R2, R4, and R5 are selected so that there is at least one primary amine group and at least one secondary or tertiary amine group in the compound, and (c) at least one corrosion inhibitor that is selected from 8-hydroxyquinoline and isomers thereof, benzotriazoles, catechol, monosaccharides, and polyhydric alcohols selected from mannitol, sorbitol, arabitol, xylitol, erythritol, alkane diols and cycloalkane diols.

The invention relates to a method for preparing lower alcohols through catalytically hydrocracking polyols, including sorbitol, mannitol, arabitol, xylitol, butantetraol, erythritol and glycerol, by taking insoluble hydroxides as additives. The method comprises the step of catalytically hydrocracking the polyols, which serve as a raw material, in an aqueous solution at the temperature of 150 DEG C to 320 DEG C and the H2 pressure of 1MPa to 20MPa in a manner of taking one or more than two of transition metals such as platinum, ruthenium, palladium, rhodium, nickel, copper, manganese and tungsten as catalyst active ingredients and taking the insoluble hydroxides such as cobalt hydroxide, aluminum hydroxide, iron hydroxide, cerium hydroxide, lanthanum hydroxide and yttrium hydroxide as adjuvants, so as to prepare the lower alcohols such as ethylene glycol and propylene glycol. The method has the advantages that the hydrocracking of the polyols is achieved by adding the insoluble hydroxides as the adjuvants, and the insoluble hydroxides are insoluble in water and are easy in separation; and after a reaction ends, a reaction system is not required to be neutralized by using additional acids.

The invention discloses application of three metabolic markers independently or jointly in preparation of a kit for diagnosing systemic lupus erythematosus. The metabolic markers which are arabitol, asparagine and stearic acid provided by the invention can be used for diagnosing and distinguishing healthy people and systemic lupus erythematosus patients independently or by combining any two or three of the metabolic markers, and the diagnosis accuracy is high, so that the metabolic markers have the prospect of being developed and prepared into a kit for diagnosing systemic lupus erythematosus. In addition, the diagnostic index provided by the invention is serum metabolite, can be detected only by taking a small amount of blood, and is basically noninvasive.

The invention discloses a strain for producing D-arabitol and application of the strain, belonging to the technical field of biology. The invention provides Candida parapsilosis SK26.002A6 obtained bymutation breeding. The Candida parapsilosis SK26.002A6 is preserved in the China Center for Type Culture Collection (CCTCC) at present, with an accession number of CCTCC NO: M2019518. The strain canproduce D-arabitol at high yield; a production method has the advantages of mild production conditions, environmental friendliness, low cost and the like, and a new method is provided for industrial preparation of D-arabitol; and the D-arabitol produced by the method is safe and reliable, is a functional product with great market potential, and has good application prospects in the fields of food,medicines, chemical engineering and the like.