Method for preparing xylitol and its intermediate D-xylosone by microbial transformation of glucose and strain used in the same

A technology of microbial transformation and Gluconobacter oxidans, applied in the field of xylitol intermediates, can solve the problems of low efficiency of arabitol

Active Publication Date: 2012-11-28
SHANDONG TIANLI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The present invention aims at the defect of low efficiency of converting glucose into arabitol in the prior art, and provides a method for microbial conversion of glucose to prepare D-xylulose. The yield of ketose is effectively improved

Method used

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  • Method for preparing xylitol and its intermediate D-xylosone by microbial transformation of glucose and strain used in the same
  • Method for preparing xylitol and its intermediate D-xylosone by microbial transformation of glucose and strain used in the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] (1) Medium: The composition of seed medium A is: glucose 80 g / L, corn steep liquor 10 g / L, yeast extract 3 g / L, MgSO 4 0.5 g / L, natural pH; composition of seed medium B: arabitol 120 g / L, yeast extract 10 g / L, calcium carbonate 1.5 g / L, pH 5.2; fermentation medium composition: glucose 160 g / L L, corn steep liquor 10 g / L, yeast extract 3 g / L, KH 2 PO 4 3 g / L, MgSO 4 0.5 g / L, pH 5.0.

[0055] (2) Seed liquid preparation: a) Kodamaea ohmeri TL-1123 seed liquid: Take a ring of bacteria from the slope and inoculate it in a shaker flask (medium A), with a liquid volume of 15%, 250 rpm , cultivated at 35°C for 16 h as the seed solution of the yeast shake flask (that is, the first-grade seed solution, the same below), and then inoculated the yeast shake flask seed solution into the 100 L fermenter seed solution (medium A) according to the inoculum amount of 10%. , with a liquid volume of 60%, an aeration ratio of 0.5 L / L min, 150 rpm, and expanded culture at 35°C for 14 ...

Embodiment 2

[0063] (1) Medium: The composition of seed medium A is: glucose 70 g / L, corn steep liquor 8 g / L, yeast extract 4.5 g / L, MgSO 4 0.9 g / L, natural pH; composition of seed medium B: arabitol 110 g / L, yeast extract 7 g / L, calcium carbonate 1.2 g / L, pH 5.1; fermentation medium composition: glucose 150 g / L L, corn steep liquor 8 g / L, yeast extract 4 g / L, KH 2 PO 4 3.5 g / L, MgSO 4 0.9 g / L, pH 5.3.

[0064] (2) Seed solution preparation: a) Kodamaea ohmeri TL-1123 seed solution: Take a ring of bacteria from the slope and inoculate it in a shaker flask (medium A), with a liquid volume of 15%, 180 rpm , cultured at 33°C for 18 h as the yeast shake flask seed solution, and then inoculated the yeast shake flask seed solution in a 100 L fermenter seed solution (medium A) according to the inoculum volume of 15%, with a liquid volume of 60% and an aeration ratio of 0.8 L / L min, 100 rpm, expanded culture at 33°C for 12 h as yeast expansion seed solution; b) Gluconobacter oxidans seed so...

Embodiment 3

[0072] (1) Medium: Seed medium A is: glucose 60 g / L, corn steep liquor 5 g / L, yeast extract 5 g / L, MgSO 4 1.5 g / L, natural pH; composition of seed medium B: arabitol 100 g / L, yeast extract 5 g / L, calcium carbonate 0.5 g / L, pH 5.4; fermentation medium composition: glucose 120 g / L L, corn steep liquor 5 g / L, yeast extract 5 g / L, KH 2 PO 4 4 g / L, MgSO 4 1.5 g / L, pH 5.5.

[0073] (2) Seed solution preparation: a) Kodamaea ohmeri TL-1123 seed solution: Take a ring of bacteria from the slope and inoculate it in a shaker flask (medium A), with a liquid volume of 15%, 100 rpm , cultivated at 30°C for 20 h as yeast shake flask seed solution, and then inoculated the yeast shake flask seed solution in a 100 L fermenter seed solution (medium A) according to the inoculum amount of 5%, with a liquid volume of 60% and an aeration ratio of 0.3 L / L min, 200 rpm, 30°C expanded culture for 20 h as yeast expansion seed solution; b) Gluconobacter oxidans seed solution: all colonies were elu...

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Abstract

The invention discloses a method for preparing D-xylosone by microbial transformation of glucose, comprising the following steps: firstly, respectively preparing an osmophilic yeast seed liquid and a gluconobacter oxydans seed liquid by using glucose as a raw material, then inoculating the osmophilic yeast seed liquid to conduct arabitol fermentation, and then in middle and later stage inoculating the gluconobacter oxydans seed liquid to conduct mixed culture fermentation, simultaneously controlling the glucose content in the broth to be 5-10g/L, and converting arabitol into D-xylosone. According to the method, the feedback inhibition of arabitol is removed, and the efficiency of preparing D-xylosone from glucose is raised. The invention further discloses a method for preparing xylitol by microbial transformation of glucose, comprising the following steps: firstly converting glucose into D-xylosone by the above method, then converting D-xylosone into D-xylose by isomerization of enzyme, extracting and refining, and conducting catalytic hydrogenation to obtain xylitol. According to the method, the production efficiency of xylitol is raised, and the cost of preparing xylitol by biological method is reduced.

Description

technical field [0001] The present invention relates to a preparation method of a xylitol intermediate——D-xylulose, a method of using the intermediate to prepare xylitol and the strains used, in particular to a method for preparing xylitol by using glucose as a raw material through microbial conversion. The method of D-xylulose and xylitol and the strains used. Background technique [0002] Xylitol belongs to five-carbon polyols and is a natural sweetener. Xylitol is not used by bacteria in the oral cavity and has excellent anti-caries function. On the other hand, xylitol does not require the participation of insulin, and can directly enter human cells to metabolize and replenish energy without causing blood sugar to rise, and can alleviate the symptoms of excessive drinking, polyphagia and polyuria in diabetic patients. The sweetness of xylitol is equivalent to that of sucrose, but it has lower calories than sucrose; compared with other sugar alcohols, it has the highest ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P19/02C12P7/18C12N1/16C12R1/01C12R1/645
Inventor 张全景付吉明王乔隆郑秀宁刘敏庄祎
Owner SHANDONG TIANLI PHARMA
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