90results about How to "Increase stringency" patented technology

The invention provides polypeptides having IgG Fc regions with amino acid modifications that result in the polypeptides exhibiting altered Fc effector functions.

This invention is directed to a process for irreversibly binding nucleic acid to solid phase and corresponding processes for the utilization thereof. Nucleic acid is bound to solid phase matrixes exhibiting sufficient hydrophilicity and electropositivity to irreversibly bind the nucleic acids from a sample. These processes include nucleic acid (double or single stranded DNA and RNA) capture from high volume:low concentration specimens, buffer changes, washes, and volume reductions, and enable the interface of solid phase bound nucleic acid with enzyme, hybridization or amplification strategies. The invention, solid phase irreversibly bound nucleic acid, may be used, for example, in repeated analyses to confirm results or test additional genes in both research and commercial applications. Further, a method is described for virus extraction, purification, and solid phase amplification from large volume plasma specimens.

Compositions for targeted mutagenesis of cell surface receptors for HIV and methods of their use are provided herein. The compositions include triplex-forming molecules that displace the polypyrimidine strand of target duplex and form a triple-stranded structure and hybrid duplex in a sequence specific manner with the polypurine strand of the target duplex. The triplex-forming molecules include a mixed-sequence “tail” which increases the stringency of binding to the target duplex, improves the frequency of modification at the target site, and reduces the requirement for a polypurine:polypyrimidine stretch. Methods for using the triplex-forming molecules in combination with one or more donor oligonucleotides for targeted modification of sites within or adjacent to genes that encodes cell surface receptors for human immunodeficiency virus (HIV) are also disclosed. Methods for ex vivo and in vivo prophylaxis and therapy of HIV infection using the disclosed compositions are also provided.

DNA-based methods employing amplification primers or probes for detecting, identifying, and quantifying in a test sample DNA from (i) any bacterium, (ii) the species Streptococcus agalactiae, Staphylococcus saprophyticus, Enterococcus faecium, Neisseria meningitidis, Listeria monocytogenes and Candida albicans, and (iii) any species of the genera Streptococcus, Staphylococcus, Enterococcus, Neisseria and Candida are disclosed. DNA-based methods employing amplification primers or probes for detecting, identifying, and quantifying in a test sample antibiotic resistance genes selected from the group consisting of blatem, blarob, blashv, blaoxa, blaZ, aadB, aacC1, aacC2, aacC3, aacA4, aac6'-lla, ermA, ermB, ermC, mecA, vanA, vanB, vanC, satA, aac(6')-aph(2''), aad(6'), vat, vga, msrA, sul and int are also disclosed. The above microbial species, genera and resistance genes are all clinically relevant and commonly encountered in a variety of clinical specimens. These DNA-based assays are rapid, accurate and can be used in clinical microbiology laboratories for routine diagnosis. These novel diagnostic tools should be useful to improve the speed and accuracy of diagnosis of microbial infections, thereby allowing more effective treatments. Diagnostic kits for (i) the universal detection and quantification of bacteria, and / or (ii) the detection, identification and quantification of the above-mentioned bacterial and fungal species and / or genera, and / or (iii) the detection, identification and quantification of the above-mentioned antibiotic resistance genes are also claimed.

The invention relates to isolated polynucleotides homologous with a portion of one strand of the human tumor suppressor gene, FEZ1, and to the tumor suppressor protein encoded thereby, Fez1. The polynucleotides are useful, for example, as probes, primers, portions of expression vectors, and the like. The invention also includes diagnostic, therapeutic, cell proliferation enhancement, and screening methods which involve these polynucleotides and protein. The invention further includes kits useful for performing the methods of the invention.

The invention relates to isolated polynucleotides homologous with a portion of one strand of the human tumor suppressor gene, FEZ1, and to the tumor suppressor protein encoded thereby, Fez1. The polynucleotides are useful, for example, as probes, primers, portions of expression vectors, and the like. The invention also includes diagnostic, therapeutic, cell proliferation enhancement, and screening methods which involve these polynucleotides and protein. The invention further includes kits useful for performing the methods of the invention.

The invention relates to methods and corresponding arrays especially suited to correct for signal errors due to variations in sample preparation. Methods and compositions for performing quantitative array-based assays are provided. In the subject methods, both a reporter and an analyte is employed, where the reporter is characterized by binding selectively to an internal reference present on the array, i.e. at least a subset of, if not all of, the spots present on the array employed in the method contain an internal reference which can be bound by reporter.

The invention features a method of inhibiting tumor growth and / or tumor invasiveness in a mammal by administering to a mammal a compound (e.g., an antagonistic antibody) which inhibits expression or enzymatic activity of human aspartyl (asparaginyl) beta-hydroxylase (HAAH). The invention also features a method for diagnosing the growth of a malignant neoplasm (e.g., pancreatic cancer) in a mammal by contacting a tissue or bodily fluid from the mammal with an antibody which binds to a HAAH polypeptide under conditions sufficient to form an antigen-antibody complex and / or detecting the antigen-antibody complex.

The present invention relates to methods, agents and compound screening assays for inducing differentiation of undifferentiated mammalian cells into osteoblasts. The invention thus provides a method, comprising contacting a compound with a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID No: 194-309; and measuring a compound-polypeptide property related to the differentiation of said cells. The invention further relates to a bone formation enhancing pharmaceutical composition, and the use thereof in treating and / or preventing a disease involving a systemic or local decrease in mean bone density in a subject. Furthermore, the invention relates to a method for the in vitro production of bone tissue.

Conjugates between a minor groove binding molecule, such as the trimer of 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI3), and an oligonucleotide form unusually stable hybrids with complementary target sequences, in which the tethered CDPI3 group resides in the minor groove of the duplex. These conjugates can be used as probes and primers. Due to their unusually high binding affinity, conjugates as short as 8-mers can be used as amplification primers with high specificity and efficiency. MGB conjugation also increases the discriminatory power of short oligonucleotides, providing enhanced detection of nucleotide sequence mismatches by short oligonucleotides. The MGB-conjugated probes and primers described herein facilitate various analytic and diagnostic procedures, such as amplification reactions, PCR, detection of single-nucleotide polymorphisms, gene hunting, differential display, fluorescence energy transfer, hydrolyzable probe assays and others; by allowing the use of shorter oligonucleotides, which have higher specificity and better discriminatory power.

The present disclosure provides antibody sequences found in antibodies that bind to human CD25. In particular, the present disclosure provides sequences of anti-human CD25 antibodies, which do not block the binding of CD25 to IL-2 or IL-2 signalling. Antibodies and antigen-binding portions thereof including such sequences can be used in pharmaceutical composition and methods of treatment, in particular for treating cancer.

Provided are methods for selectively identifying and isolating nucleic acids in a population of nucleic acid molecules.

The invention provides a peptide having at least 3 amino acids comprising an amino acid sequence selected froma)X1SM[SEQ.ID.NO.: 1]b)LX2HK[SEQ.ID.NO.: 2]c)PSGX3ARA[SEQ.ID.NO.: 9]d)SX4RSMNF[SEQ.ID.NO.: 16]e)LX5HKSMP[SEQ.ID.NO.: 18]in which X1 is a basic amino acid residue, X2 is Q or P, X3 is A or T, X4 is an acidic amino acid residue and X5 is P or Q.The invention further provides non-viral cell-targeting vector complexes and methods associated therewith.

The invention relates to a monitoring system for coal mine employed person safety training. Reasons for causing frequent occurrence of unsafe behaviors of coal mine employed persons are as follows: the qualities of employed persons are different, and scientific and effective management on the employed persons is lack; the scientificity and the effectiveness for training are not enough, the training means is single, and the training effect is lack of evaluating and monitoring; safety monitoring is lack of effective methods and means, and person training is out of line with monitoring. The monitoring system comprises a local side database, management servers, handheld terminals and touch screen training and examining integrated machines, wherein a local side server is connected with a plurality of the touch screen training and examining integrated machines arranged below the local side server through the Internet, and the management servers and the handheld terminals are connected between the local side server and each of the touch screen training and examining integrated machines. The monitoring system is beneficial to the improving of the positivity and the convenience for the employed persons to initiatively take part in training and learning, ensures the promptness and the accuracy of a monitoring process, and greatly improves the accuracy, the real-time and the comprehensiveness of monitoring management.

The invention provides methods and compositions for performing a stringent wash step in a hybridization assay between at least one molecule and a target. The invention may, for example, eliminate the use of, or reduce the dependence on formamide in the stringent wash step. Compositions for use in the invention include an aqueous composition comprising at least one polar aprotic solvent in an amount effective to denature non-complementary sequences in a hybridization product.

The present invention provides methods for producing recombinant peptides in a bacterial host utilizing a mannitol, arabitol, glucitol, or glycerol-inducible promoter, wherein the host bacterial cell that produces the peptide has been rendered incapable of degrading or metabolizing mannitol, arabitol, or glucitol, or derivatives or analogues thereof. The present invention provides bacterial cells that have been genetically altered to inhibit the metabolism or degradation of mannitol, glucitol, or arabitol, or derivatives or analogues thereof. The present invention utilizes mannitol, arabitol, glucitol, or glycerol to induce expression of a target polypeptide from an inducible promoter, allowing for the use of an inexpensive and stable carbon source inducer in the fermentation processes for the production of recombinant peptides.

The invention discloses an alarm strategy automatic adjustment method and system for an optical fiber grating perimeter security protection system. The method includes the following steps that: S1, the trigger recording variables of sensing units in the optical fiber grating perimeter security system are initialized; S2, demodulated external vibration signals are received; S3, judgment is carriedout according to the demodulated external vibration signals, if at a certain moment, the signal disturbance range of a certain sensing unit exceeds a threshold, the sensing unit is triggered; S4, information in the trigger recording variable of the triggered sensing unit is updated; S5, a corresponding alarm strategy is automatically assigned to the triggered sensing unit according to the number of the times of the cumulative triggering of the triggered sensing unit in a previous period, if a corresponding alarm condition is met after calculation is performed, and alarm information is outputted. With the alarm strategy automatic adjustment method and system of the invention adopted, high-sensitivity alarm in a quiet environment can be realized; and the strictness of alarm judgment in an environment with large disturbance such as wind and rain can be improved. The alarm strategy automatic adjustment method and system are suitable for a windy environment, can improve an alarm accuracy rate, and reduce wind and rain false alarm rates.

The present invention provides methods for detecting the presence of pathogenic moulds in biological samples that are based on amplification of mould nucleic acids. The methods may further comprise quantitating and real time detection of the mould. The methods of the invention are highly specific and do not co-amplify human or other yeast nucleic acids. The methods of the invention are also extremely sensitive. Thus, methods for diagnosing infections caused by mould are provided. The invention also provides kits for detection of moulds.

The invention provides a transgenic plant having increased expression of a positive regulator protein of systemic acquired resistance (SAR), thereby enhancing the SAR response and pathogen resistance of the plant. The positive regulator protein is a component of a signal transduction pathway leading to (SAR), and is a MAP kinase protein (MPK4) substrate, and interacts with transcription factors.

In the present invention, information cue module outputs combination of cue information in sentence databank according to current difficulty grade, result comparison module carrys on real time judgement for the sentence inputted by the learner and to send the compared result to difficulty grade regulation module, the difficulty grade regulation module regulates the difficulty grade to increase matching stringency for study of typewriting and sentnece-making as well as multiinformation combination cue is applied to raise sentnece organizatino ability and spelling-typewriting ability of the learner.

Disclosed herein are methods, probes, and kits for diagnosing the presence of cancer and / or a cancer type in a subject.

The invention relates to the field of medicine circulation and discloses a medicine graphic and text information violation detection method and system which can rapidly and accurately detect and screen out medicine graphic and text information violating management specifications. The method comprises the following steps of: obtaining a candidate picture and text description of a medicine; extracting a medicine packaging area from the candidate picture; carrying out OCR identification on the medicine packaging area to obtain a first identification result; under the condition that a preset character string exists in the first identification result, respectively calculating the similarity between each recorded advertisement picture in a retrieval result and the candidate picture according tothe text description or a universal name in the first identification result and the recorded advertisement pictures under the retrieval of a production enterprise; and under the condition that no similarity exceeds a first preset threshold value, judging that the advertisement picture is illegal; and extracting multiple pieces of medicine information from the first identification result, comparingthe multiple pieces of medicine information with multiple pieces of medicine information extracted from the text description one by one, and under the condition that at least one piece of medicine information is inconsistent, judging that the medicine information is illegal.

Disclosed is a method for the selection of aptamers that does not require immobilization of the target or the oligonucleotide library. The method includes: (i) combining a selection library of oligonucleotides that contain a random region flanked by primer recognition sites with blocker oligonucleotides that anneal to the primer recognition sites; (ii) exposing the blocked selection library to an immobilization field including random oligonucleotides and removing unbound selection library oligonucleotides; (iii) recovering the selection library oligonucleotides that bound to the immobilization field and combining with a target analyte of interest; (iv) exposing the selection library oligonucleotides-target analyte mixture to an immobilization field; and (v) recovering those selection library oligonucleotides that did not bind to the immobilization field.

The present invention relates to a system and method for controlling peptide display valency on virus-like particles (VLPs), especially including MS2 or PP7 VLPs. In this method, large amounts of wild-type and low quantities of single-chain dimer coat proteins may be produced from a single RNA. Valency is controlled in immunogen (vaccine) production by providing a system that allows the production of large amounts of wild-type and low quantities of single-chain dimer coating proteins from a single RNA, allowing facile adjustment of display valency levels on bacteriophage VLPs, especially MS2 or PP7 VLPs over a wide range, from few than one—on average—to as many as ninety per particle. This facilitates the production of immunogens and vaccines, including VLPs exhibiting low valency. Nucleic acid constructs useful in the expression of virus-like particles are disclosed, comprised of a coat polypeptide of bacteriophage such as MS2 or PP7 modified by insertion of a heterologous peptide, optionally comprising a carbohydrate mimotope, wherein the heterologous peptide is displayed on the virus-like particle and encapsidates bacteriphage mRNA.

Provided are: a selection method for a gene switch and a gene circuit, including using, as a selector, an expression vector containing at least a gene sequence whose expression is controlled by a transcription regulatory factor to be expressed when a genetic switch and a genetic circuit including the genetic switch operate, and a promoter sequence operably linked to the gene sequence upstream thereof; and an expression vector to be used in the selection method. This enables an effective selection method for a genetic switch and a genetic circuit, the selection method being able to be conducted within a short time period and with high selection efficiency and less leakiness.

Methods and compositions for detecting, identifying, and quantifying Brassica A genomic DNA are described. The methods are specific to the Brassica A genome and do not cross-react with other Brassica species, crops or weedy relatives that could contribute to contamination of a canola field.

The disclosure concerns compositions and methods for determining the zygosity of corn plants containing one or more floury2 (fl2) mutations. In embodiments, the disclosure concerns a gene specific PCR-based molecular (KASPar®) assay for identifying the floury2 trait in plants. In certain embodiments, compositions and methods are disclosed for determining the zygosity of corn plants with respect to the fl2 allele. In particular embodiments, the assay may be used for fl2 germplasm identification, accelerating introgression and molecular breeding programs in corn and other plants.

The present invention provides methods for producing recombinant peptides in a bacterial host utilizing a mannitol, arabitol, glucitol, or glycerol-inducible promoter, wherein the host bacterial cell that produces the peptide has been rendered incapable of degrading or metabolizing mannitol, arabitol, or glucitol, or derivatives or analogues thereof. The present invention provides bacterial cells that have been genetically altered to inhibit the metabolism or degradation of mannitol, glucitol, or arabitol, or derivatives or analogues thereof. The present invention utilizes mannitol, arabitol, glucitol, or glycerol to induce expression of a target polypeptide from an inducible promoter, allowing for the use of an inexpensive and stable carbon source inducer in the fermentation processes for the production of recombinant peptides.

The invention pertains to a method for selecting at least one eukaryotic host cell expressing a product of interest, comprising(a) providing a plurality of eukaryotic host cells, wherein cellular viability of said host cells is dependent upon folate uptake, wherein said host cells comprise at least(i) a foreign polynucleotide encoding a product of interest and(ii) a foreign polynucleotide encoding a DHFR enzyme;(b) culturing said plurality of eukaryotic host cells in a selective culture medium comprising at least an inhibitor of DHFR and folate in a limiting concentration; and(c) selecting at least one eukaryotic host cell expressing the product of interest.Also provided is a method for expressing a product of interest which is based on host cells selected by said method and a cell culture medium.