138results about How to "Reduce salt concentration" patented technology

The invention relates to a deep treatment process of landfill leachate. The effluent of landfill leachate subjected to biochemical treatment is used as influent. The process adopts a coagulative precipitation tank, an oxidation tank, an anaerobic biological filter and a membrane treatment device and comprises the following steps of: (1) removing partial organic matter through coagulative precipitation to reduce chroma; (2) further removing refractory and non-biochemical organic matter with an oxidizer formed by ozone, and the like and improving the biodegradability of the effluent; (3) removing SS (Suspended Solid), organic matter and nitrate in the leachate through an anaerobic filter; and (4) ensuring that the effluent stability of the whole system through a membrane separation technology reaches the emission standard and canbe recyclable. The process has the advantages of lower operation cost, stable effluent, less quantity of generated concentrated liquid, less salt content in theconcentrated liquid, and the like, and the process of biochemistry and materialization of the pretreatment, which is used before using the membrane method, can prolong the service life of the membrane.

The invention relates to an in-situ rapid preprocessing method for protein samples. The whole preprocessing process of the protein samples is carried out in a centrifugal ultrafiltration device in situ, the denaturation process, the reduction process, the solvent replacement process, the alkylate process, the enzymolysis process and the impurity removal process of the protein samples are integrated, and enzymolysis of the protein samples is accelerated under assistance of microwaves. The method can remarkably improve the preprocessing flux of the protein samples; meanwhile, the whole preprocessing process of the samples is carried out in situ, and the recovery rate is high. In addition, the centrifugal ultrafiltration device can conveniently and rapidly achieve the purification of the samples and replacement of solvents, and enzymatic hydrolysate does not need an additional purification process and can be directly combined with subsequent efficient liquid chromatogram separation and mass spectrum identification.

The present invention relates generally to the fields of genomics, synthetic biology and genetic engineering. More particularly, the present invention concerns the methods that enable parallel multiplex ligation and amplification on surface for making assemblies of nucleic acids of various biological applications and for analysis of biological samples such as DNA, RNA, and proteins.

The invention provides a method of pickling salty preserved eggs. The salty preserved eggs are formed by pickling salted eggs and preserved eggs through a sectional type design. According to the cooked salty preserved eggs, egg white is yellowish-brown preserved-egg gel and is crystal clear and high in resilience, and the salty preserved eggs have a preserved egg flavor; yolks are floppy, can produce oil and have the salted egg feature; one duck egg has two flavors.

The invention relates to an ammonium fluoride wastewater treatment technique which comprises the following steps: ammonium fluoride wastewater pretreatment, ultrafiltration treatment, electrodialysis treatment, and nanofiltration treatment until the ammonium fluoride content of the ammonium fluoride solution subjected to nanofiltration treatment is less than 5 mg/L, thereby completing the ammonium fluoride wastewater treatment. The invention has the following advantages: 1. reasonable technique combination: the ultrafiltration technique is adopted, the wastewater is treated by internal circulation between the electrodialysis system and the nanofiltration system according to the concentration of ammonium fluoride, and the electrodialysis technique desalts and concentrates inorganic salts in the wastewater, and has high permeation speed and desalting performance; 2. the investment and operating cost is lowered; and 3. ammonium salts in the wastewater are treated in good time, thereby changing wastes into valuable substances and implementing zero discharge to the outside.

The invention relates to a resource comprehensive utilization method for recovering fluorine from wet-process phosphoric acid. The method comprises the following steps of: absorbing SiF4 and HF gases generated in the wet-process phosphoric acid by using water, and preparing the gases into a hydrofluosilicic acid solution of which the mass percent concentration is 10-35%; mixing one of four solids including solid sodium sulfate, solid potassium sulfate, solid sodium chloride and solid potassium chloride with the hydrofluosilicic acid solution, and reacting for 0.1-2 hours at 30-80 DEG C under stirring to obtain a mixture; centrifugally separating the obtained mixture to obtain a solid and a filtrate; and washing the solid with water, and drying for 1-3 hours at 100-120 DEG C to obtain a sodium fluorosilicate or potassium fluorosilicate crystal. According to the method, a byproduct hydrofluosilicic acid in the wet-process phosphoric acid and sodium (potassium) sulfates or chlorides which are abundant and easily-available are used as raw materials, so that the cost is low, two products including sodium fluorosilicate and potassium fluorosilicate can be produced at the same time by using one set of equipment, and the maximum utilization ratio of the hydrofluosilicic acid can reach over 99%.

The invention discloses a low-cost iron phosphate ammonia-nitrogen-containing wastewater treatment method, which comprises: adding an appropriate amount of Ca(OH)2 to an iron phosphate mother liquor,adjusting the pH value to 6-8 to form a calcium sulfate precipitate, and filtering the precipitate to obtain a first mixed liquid, wherein the first mixed liquid mainly contains NH4<+>, SO4<2-> and PO4<3->; carrying out first membrane concentration on the first mixed liquid to obtain a second mixed liquid; carrying out MVR evaporation on the second mixed liquid, and separating to obtain an ammonium sulfate crystal and an ammonium phosphate crystal; and washing the crystals, and drying to obtain the ammonium sulfate finished product and the ammonium phosphate finished product. According to thepresent invention, the process of the method is simple, and the used raw material is Ca(OH)2, and Ca(OH)2 can reduce the salt concentration in the system, such that the amount of the water entering the MVR evaporation can be reduced so as to correspondingly reduce the evaporation cost; and Ca(OH)2 is a solid, and cannot increase the amount of the water in the system so as to reduce the treatment capacity of the first membrane concentration system and the treatment cost.

The invention relates to a method for fermenting and generating Ectoine by utilizing microorganisms, which belongs to the microorganism fermentation engineering filed. The method comprises the following steps: fermenting a plurality of strains of Halomonas, which can secrete Ectoine, in a liquid culture medium with glutamic acid mono sodium as a sole carbon nitrogen source and the NaCl concentration being 15 to 120g/L; inducing synthesis and enaling Ectoine to be secreted; and recycling the Ectoine in a fermentation system. The method ensures that the fermentation productivity of the Ectoin reaches 6.4 to 7.9g/L/d, which is 2.1 to 2.4 times of that of the prior art, and products are mostly secreted into the culture medium, so as to simplify the recycling process of the products, and to be favor of realizing continuous culture. Compared with the prior art, the NaCl concentration in the culture medium is reduced by 1/2 to 2/3, thus being favor of reducing the corrosion of equipment, and reducing the downstream processing cost.

The invention provides a furrow-sowing drip-irrigation mulch-cultivation method of a salinized cotton field in an arid area, including furrowing and ridging, and double-row planting in furrows. The method is characterized in that ridges are big while the furrows are small, a drip irrigation zone laid in the centerline of a furrow bottom is mulched thereon by a plastic film, and bunch planting is performed at both sides of the drip irrigation zone on the plastic film. The plastic film mulching mode includes single-film mulching and double-film mulching, wherein the single-film mulching is to mulch a film in ridge bare furrows or mulch a film evenly in the furrows, and the double-film mulching is to mulch a top film tightly on the top surface of an adjacent ridge after planting based on the single-film mulching so as to form a furrow chamber. Since the evaporation on the ridges is relatively stronger than that in the furrows, salt is allowed to transfer and accumulate above the ridges, and the rhizosphere salinity of the furrows is reduced accordingly. The method realizes a combination of ridging and furrow sowing, plastic film mulching and water-saving drip irrigation, thus water saving, warming and salt reducing effects are achieved, high yield of cotton is facilitated, and increased yield rate ranges from 8.3% to 16.4%. Compared with the conventional salt-alkali improvement measures, the method features more water conservation, lower investment, and easier operation.

The invention discloses a culture medium for promoting an adventive root of a woody plant to root and grow and an application of the culture medium. The culture medium comprises the following components: inorganic salt A, inorganic salt B, an organic compound, 50-150mg/L water-soluble carbon nano tubes, 0.5-1.5mg/L indolebutyric acid, 100mg/L 2-(N-morpholine) ethanesulfonic acid, 30000mg/L cane sugar and 5000mg/L agar. The culture medium provided by the invention is more capable of promoting the growth of the adventive root. The largest difficulty of asexual reproduction of the woody plant lies in the rooting and the growth of the adventive root, the culture medium has the same promotion effect for the rooting of the adventive root of brassaia actinophylla and the rooting and the growth of plants such as honeysuckle, blueberry, small kiriko, swida wilsoniana, zanthoxylum dissitum and the like.

The invention relates to a method for fast enzymolysis of hybrid proteins comprising: providing protein samples to be enzymolysis; obtaining the denatured protein samples after the degeneration treatment of said protein samples; treating said denatured protein samples by means of protease enzymolysis and assistant with ultrasonic, obtaining the protein polypeptide fragments. The invention also provides a method for fast identifying the protein. The method of the invention can quickly and high efficiently implement the protein enzymolysis and identification with easy operation and high accuracy.

The invention discloses a separation and purification method for a monoclonal antibody. The method includes the steps that cell culture supernatant containing the monoclonal antibody is subjected to Protein A affinity chromatography so as to obtain a first elution component; the first elution component is placed for a specified time at the room temperature with a specified pH value kept so as to obtain the first elution component subjected to low-pH-value inactivated virus treatment; the first elution component subjected to low-pH-value inactivated virus treatment is neutralized and then subjected to cation exchange chromatography so as to obtain a second elution component; the second elution component is diluted and then subjected to anion exchange chromatography so as to obtain a through flow; the through flow is filtered with a virus removal filter so as to obtain filter liquor; the filter liquor is subjected to ultrafiltration concentration, filter wash and buffer solution replacement in sequence so as to obtain stoste of the monoclonal antibody. The method is simple and easy to implement, and the endotoxin content and other quality indexes of the obtained product meet the quality standard of monoclonal antibody drugs.

A process for producing soda ash from brine waste, the process including reacting brine waste with carbon dioxide and ammonia to produce soda ash, where in at least a portion of the ammonia is regenerated from the ammonium chloride produced during the reaction, the regeneration ideally be achieved by the use of a weak base anion exchange resin.

The invention discloses a green alga and viable bacterium compound, a method for preparing the same and application of the green alga and viable bacterium compound. The green alga and viable bacteriumcompound comprises, by weight, 40-60 parts of green algae and 10-20 parts of viable bacteria. The green algae are chlorella, and the viable bacteria are photosynthetic bacteria and bacillus polymyxa.The green alga and viable bacterium compound, the method and the application have the advantages that the green alga and viable bacterium compound can be used in the agricultural field; the bacteriaand the algae are complementary with one another, and accordingly the green alga and viable bacterium compound is environmentally friendly; effects of promoting growth of crops, improving the diseaseresistance of the crops, increasing the yield of the crops and improving the quality of the crops can be realized; the green alga and viable bacterium compound can be used as a foliar fertilizer, accordingly, the photosynthetic rates of the crops can be increased by 2-3 times, nitrogen sources and carbon dioxide in air can be sufficiently utilized, energy can be saved, and environments can be protected; effects of remedying soil further can be realized by the green alga and viable bacterium compound, and effects of immobilizing heavy metal, degrading harmful substances such as polycyclic aromatic hydrocarbons, reducing the salt concentration and improving the water retention and fertilizer conservation capacity of the soil can be realized by the green alga and viable bacterium compound.

The invention discloses a technology for normal-temperature salt-free extraction of heparin sodium. The technology comprises the following steps of breaking intestinal mucosa, carrying out enzymolysis by a protease-lipase-nuclease compound enzyme preparation under the conditions of a normal temperature and no salt, then carrying out clear liquid centrifugal collection, adsorbing heparin sodium in the centrifugate by appropriate ion exchange resin, carrying out elution on the filtrate by high-concentration salt, precipitating the heparin sodium in the eluent by alcohol and carrying out drying to obtain a heparin sodium crude product. The technology can save a large amount of heat energy in enzymolysis and adsorption, reduce energy consumption and reduce a production cost. The technology realizes heparin sodium enzymolysis and adsorption under the condition of no salt so that the salinity of the discharged waste water is reduced, a waste water treatment cost is reduced and environmental benefits are improved.