31results about How to "Reduce preprocessing steps" patented technology

The invention discloses a method for ultrasonic assisted conversion of chitin into chitosan. The method comprises the following steps: (1) adding deionized water into lucid ganoderma spore powder, carrying out ultrasonic extraction in a water bath, then adding ethanol, carrying out ultrasonic extraction at a room temperature, carrying out centrifuging, then carrying out soaking with deionized water, and carrying out centrifuging and drying so as to obtain crude chitin; (2) placing the crude chitin in a H2O2 aqueous solution, maintaining the water bath until decolorizing of the crude chitin iscompleted, adjusting a pH value of a decolorized chitin solution to neutral with a NaOH solution, and carrying out centrifuging so as to obtain a precipitate; and (3) adding an alkali solution into the precipitate, subjecting a mixed solution to ultrasonic treatment so as to complete a deacetylation reaction, adjusting a pH value of an obtained solution to neutral with a HCl solution, then carrying out centrifuging so as to obtain a precipitate, and carrying out drying so as to obtain a crude chitosan product. The method provided by the invention uses NaOH with a small amount and a low concentration and combines an ultrasonic process to prepare the chitosan at a room temperature; in addition, the method fully utilizes residue by-products, broadens the source of the chitosan, and is applicable to scale production of the chitosan.

The invention relates to a method for fast enzymolysis of hybrid proteins comprising: providing protein samples to be enzymolysis; obtaining the denatured protein samples after the degeneration treatment of said protein samples; treating said denatured protein samples by means of protease enzymolysis and assistant with ultrasonic, obtaining the protein polypeptide fragments. The invention also provides a method for fast identifying the protein. The method of the invention can quickly and high efficiently implement the protein enzymolysis and identification with easy operation and high accuracy.

The invention discloses a method for screening active components based on a three-phase laminar flow microfluidic chip. The invention can quickly separate affinity molecules and free molecules in a short-distance microchannel to improve efficiency; and the degree of automation is high , good reproducibility, free small molecules can also be enriched and concentrated at the same time; and the effective separation of large molecules and small molecules without membranes can be completed in one step, as well as the multi-step sample pretreatment operation of molecular extraction, saving time and trouble; reducing economic costs cost, vigorously promote the process of modernization of traditional Chinese medicine.

The invention discloses a method for detecting the concentration of cycloserine in bone tuberculosis specimens. It includes the following steps: (1) freeze the bone tuberculosis specimen with liquid nitrogen, after the liquid nitrogen volatilizes, add a solvent to grind and extract cycloserine, and collect the tissue extract; (2) use high-efficiency Liquid chromatography-mass spectrometry is used for detection; when measuring, if the retention time of the chromatographic peak of the tissue extract is consistent with that of the cycloserine standard solution, and in the mass spectrum of the sample after subtracting the background, the selected qualifier ions all appear, and the abundance Consistent with the ion abundance ratio of cycloserine standard solution, it is determined that cycloserine is detected; (3) according to the data of the tissue extract determined by the high performance liquid chromatography-mass spectrometry that step (2) obtains, adopt internal standard method to The content of cycloserine in the bone tuberculosis specimen is quantitatively analyzed to obtain the concentration of cycloserine in the bone tuberculosis specimen. The invention shortens the processing time of the bone specimen and measures the concentration accurately.

The invention discloses a preparation method of a titanium oxide extraction bar for solid phase microextraction; in the method, a plurality of titanium oxide layers are deposited on the surface of polypropylene hollow fiber by adopting a sol-gel method, and then polypropylene matrix is removed by high-temperature firing, so as to obtain the titanium oxide hollow fiber extraction bar, realize extraction preenrichment to polar compounds with micro and trace amount in medicine and environment samples and carry out separation analysis to gas chromatography or high performance liquid chromatography; the extraction bar has a three-dimensional porous stricture, has larger specific surface area, controllable coating thickness, good chemical and heat stability, wide pH stabilization range (pH=1-14) and high extraction efficiency to the polar compounds.

The invention provides a pretreatment activation solution and a pretreatment method for aluminum and aluminum alloy electroplating, which include the following components: 20-30 g / L of fumaric acid, 3-5 ml / L of hydrofluoric acid, and water as a solvent. Pretreatment method: degrease and wash the aluminum and aluminum alloy workpieces, then alkali etch and wash, then pickle and wash, and finally activate with the above-mentioned pretreatment activation solution, and rinse with water after activation. The pretreatment activation solution contains composite antioxidants, which can improve the binding force of aluminum and aluminum alloy surface coatings, and can be directly electroplated after activation.