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Method for screening active components based on three-phase laminar flow microfluidic chip

A microfluidic chip and three-phase technology, applied in chemical instruments and methods, material separation, analysis of materials, etc., can solve the problems of manpower and material resources consumption, cumbersome operation, long cycle, etc., to reduce economic costs, improve separation efficiency, The effect of reducing errors

Active Publication Date: 2019-06-07
云浮市厚德中药饮片有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The separation process of the components is cumbersome and takes a long period, and the finally separated compounds may consume a lot of manpower and material resources because they have no activity or are not required by researchers.

Method used

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  • Method for screening active components based on three-phase laminar flow microfluidic chip
  • Method for screening active components based on three-phase laminar flow microfluidic chip
  • Method for screening active components based on three-phase laminar flow microfluidic chip

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Experimental program
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Effect test

Embodiment 1

[0027] A three-phase laminar flow microfluidic chip (see figure 1 and figure 2 ), including the main channel, three sample inlets and three sample outlets, the three sample inlets communicate with one end of the main channel, and the three sample outlets communicate with the other end of the main channel; the three sample inlets are respectively : Inlet 1 for sample to be tested, inlet 2 for blank buffer solution, and inlet 3 for organic extraction solvent; the three outlets are: outlet 4 for biomacromolecules that has affinity with biological target molecules, Sample outlet 5 for mixture of biomacromolecule and free drug small molecule and organic solvent sample outlet 6 (sample outlet for extracting free drug small molecule without affinity). Among them, the chip material is made of glass, the total length of the chip is 6.3cm, and the width is 2.1cm. 325μm, 30-35μm deep. The three-phase laminar flow microfluidic chip is formed by bonding the bottom glass and the cover g...

Embodiment 2

[0030] The steps of the method for screening or assisting in the screening of active ingredients are:

[0031] Take 60ml of methanol extract that has been recovered from Boluo, evaporate to dryness and redissolve in water to obtain solution I.

[0032] Mix 200 μL solution I with 200 μL target biological target molecule G-quadruplex and 600 μL blank buffer solution (50 mM KCl, 10 mM KH 2 PO 4 , and 1mM K 2 EDTA, pH 7.4) were mixed for 60 minutes, incubated at 95°C for 5 minutes, cooled naturally to room temperature, and then introduced into the sample inlet 1 of the three-phase laminar flow microfluidic chip of the present invention, and the blank buffer solution inlet 2 was simultaneously introduced into 1mL blank Buffer solution (50mM KCl, 10mM KH 2 PO 4 , and 1mM K 2 EDTA, pH 7.4) as an isolation solution, so that the biological target molecule does not come into direct contact with the organic solvent used for extraction, such as methylene chloride, so as not to affect...

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Abstract

The invention discloses a method for screening active components based on a three-phase laminar flow microfluidic chip. Affinity molecules and free molecules are separated rapidly in a short-distancemicrochannel, so that the efficiency is improved. The automation degree and the reproducibility are high; and the free micromolecules can be enriched and concentrated simultaneously. Moreover, membrane-free effective separation of macromolecules and micromolecules is completed simultaneously by one step and the multi-step sample pre-treatment operation for molecular extraction is simplified, so that the time and effort are saved. The economic cost is saved; and the process of modernization of the traditional Chinese medicine is promoted substantially.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and in particular relates to a method for screening active ingredients based on a three-phase laminar flow microfluidic chip. Background technique [0002] The composition of traditional Chinese medicine is very complex, the physical and chemical properties vary greatly, and the active ingredients of traditional Chinese medicine are not clear, and it is difficult for people to determine the curative effect according to the existing indicators. Therefore, the detection of the biological activity of traditional Chinese medicine has become a research hotspot in the quality control technology of traditional Chinese medicine. [0003] The research on effective substances of traditional Chinese medicine usually uses extraction and separation techniques (such as thin-layer chromatography, low-medium pressure column chromatography, countercurrent chromatography, and preparative chroma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/08G01N30/72G01N30/86B01L3/00
Inventor 孙悦蔡绮丹孟江王淑美梁生旺曾宇
Owner 云浮市厚德中药饮片有限公司
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