Pretreatment method for detecting nitrofuran metabolites in aquatic product

A technology of nitrofuran and aquatic products, applied in the field of analytical chemistry, can solve the problem of high cost of kits, achieve the effects of reducing pretreatment steps, improving elution effect, and shortening analysis time

Inactive Publication Date: 2012-08-01
FUJIAN CCIC FAIRREACH FOOD SAFETY TESTING
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the disadvantages of ELISA are also obvious. Generally, a kit can only detect one nitrofuran metabolite, and the cost of the kit is relatively high.

Method used

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  • Pretreatment method for detecting nitrofuran metabolites in aquatic product
  • Pretreatment method for detecting nitrofuran metabolites in aquatic product
  • Pretreatment method for detecting nitrofuran metabolites in aquatic product

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Embodiment 1

[0036] The following embodiments describe the specific working principle of the present invention in conjunction with the accompanying drawings:

[0037] The specific operation process of the pretreatment method of nitrofuran metabolite detection in the said aquatic product of the present invention is as follows figure 1 shown. The first step is to weigh and dissolve the sample to be tested. Then, the nitrofuran metabolites of the samples were derivatized by constant temperature shaking at 37 °C for 14-16 h. Next, adjust the pH of the derivatized solution to 7.0-7.5 with 1.0 mole per liter phosphate buffer solution with a pH of 7.4, and then extract the derivatized nitrofuran metabolites with ethyl acetate. This operation will separate the nitrofuran metabolites in the sample from the complex sample matrix. Finally, preparative chromatography is used to further purify the ethyl acetate extract to obtain a product that can directly enter a high-performance liquid chromatogra...

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Abstract

The invention discloses a pretreatment method for detecting nitrofuran metabolites in an aquatic product. The method comprises the following steps of: weighing a sample to be detected, dissolving, derivating, extracting, and performing chromatographic separation. A constant temperature oscillator, a reduced pressure rotary evaporator, a silica gel chromatographic column and a high speed refrigerated centrifuge are used in the method. A sample solution prepared by the method can be directly detected by liquid chromatography-tandem mass spectrometry. Compared with the conventional national standard method, the pretreatment method has the advantages of low cost, high repeatability and the like, is simply and effectively operated, is high in operability, is expected to be popularized and applied to environmental protection industry, commodity inspection, entry-exit inspection and quarantine and the like on a large scale, and is obvious in economic and social benefits.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and more specifically relates to a pretreatment method for detecting nitrofuran metabolites in aquatic products. Background technique [0002] Nitrofurans are artificially synthesized broad-spectrum antibacterial drugs with the basic structure of 5-nitrofuran. They act on microbial enzyme systems, inhibit acetyl-CoA, interfere with the metabolism of microbial sugars, and are effective against common Gram Both positive and negative bacteria have effects, and are mainly suitable for intestinal bacterial infections and protozoan diseases. Nitrofurans are sensitive to light and metabolize rapidly in vivo. Since the original drug and its residual metabolites in animals have the risk of teratogenicity, mutagenesis and carcinogenicity, due to safety considerations, developed countries such as Europe and the United States and my country have successively promulgated A ban on the use of such veterinary...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06
Inventor 陈劲星
Owner FUJIAN CCIC FAIRREACH FOOD SAFETY TESTING
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