A method for ultrasonically assisted conversion of chitin into chitosan

An ultrasound-assisted, chitin-based technology, applied in the field of chitosan, can solve the problems of ganoderma lucidum spore powder, such as poor effect, numerous preparation steps, and environmental pollution by waste liquid, and achieve the effects of broadening sources, shortening reaction time, and protecting the environment

Active Publication Date: 2020-03-31
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the indigestible nature of chitin makes the effect of oral ganoderma lucidum spore powder not good, and it is easy to cause adverse reactions such as diarrhea
[0004] At present, the large-scale production of chitosan is realized by using seafood by-products to complete the deacetylation of chitin at high concentration of strong alkali and high temperature, which has disadvantages such as time-consuming and waste liquid pollution.
Although it has been reported that the residue of polysaccharides extracted from Ganoderma lucidum fruiting bodies can be converted into chitosan, the extraction must be sliced ​​and broken, and then treated with high concentration of strong alkali and high temperature for reaction. There are many preparation steps and low efficiency. and other disadvantages

Method used

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  • A method for ultrasonically assisted conversion of chitin into chitosan
  • A method for ultrasonically assisted conversion of chitin into chitosan
  • A method for ultrasonically assisted conversion of chitin into chitosan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Take 1 g of dried Ganoderma lucidum spore powder, add deionized water to the Ganoderma lucidum spore powder according to the ratio of material to liquid 1:15 (g:ml), and extract Ganoderma lucidum polysaccharide once at 45°C with water bath ultrasonic (100W) and then use 7000rpm Centrifuge for 10 minutes to remove the supernatant to obtain the primary precipitate; add 95v / v% ethanol to the primary precipitate according to the ratio of material to liquid 1:15 (g:ml), and ultrasonicate at room temperature (25°C±1°C) for 1h to remove the three Terpenoids, and then centrifuged under the same centrifugation conditions to obtain the secondary precipitate; add deionized water to the secondary precipitate according to the ratio of solid to liquid 1:30 (g:ml), soak for 3 hours at 25°C to remove water-soluble protein, and After centrifugation under the same centrifugation conditions, the precipitate obtained three times was dried at 55°C to obtain crude chitin;

[0035] (2) Ta...

Embodiment 2

[0044](1) Take 1 g of dried Ganoderma lucidum spore powder, add deionized water to the Ganoderma lucidum spore powder according to the ratio of material to liquid 1:25 (g:ml), and extract Ganoderma lucidum polysaccharide three times at 55°C with water bath ultrasonic (150W) and then use 10000rpm Centrifuge for 5 minutes to remove the supernatant to obtain the primary precipitate; add 95v / v% ethanol to the primary precipitate according to the ratio of material to liquid 1:25 (g:ml), and ultrasonicate for 2 hours at room temperature (25°C±1°C) to remove the three Terpenoids, and then centrifuged under the same centrifugation conditions to obtain the secondary precipitate; according to the ratio of solid to liquid 1:40 (g:ml), add deionized water to the secondary precipitate, soak at 35°C for 2h to remove water-soluble protein, and After the same centrifugation, the precipitate obtained three times was dried at 60°C to obtain crude chitin;

[0045] (2) Take 1g of crude chitin, ad...

Embodiment 3

[0050] (1) Take 1 g of dried Ganoderma lucidum spore powder, add deionized water to the Ganoderma lucidum spore powder according to the ratio of material to liquid 1:30 (g:ml), and extract Ganoderma lucidum polysaccharide once at 65°C with water bath ultrasonic (200W) and then use 8000rpm Centrifuge for 10 minutes to remove the supernatant to obtain the primary precipitate; add 95v / v% ethanol to the primary precipitate according to the ratio of material to liquid 1:30 (g:ml), and ultrasonicate for 3 hours at room temperature (25°C±1°C) to remove the three Terpenoids, and then centrifuged under the same conditions to obtain the secondary precipitate; add deionized water to the secondary precipitate according to the ratio of solid to liquid 1:50 (g:ml), soak at 45 °C for 1 hour to remove water-soluble protein, and use the same After centrifugation, the precipitate obtained three times was dried at 60°C to obtain crude chitin;

[0051] (2) Get 1g of crude chitin, add 30ml concent...

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Abstract

The invention discloses a method for ultrasonic assisted conversion of chitin into chitosan. The method comprises the following steps: (1) adding deionized water into lucid ganoderma spore powder, carrying out ultrasonic extraction in a water bath, then adding ethanol, carrying out ultrasonic extraction at a room temperature, carrying out centrifuging, then carrying out soaking with deionized water, and carrying out centrifuging and drying so as to obtain crude chitin; (2) placing the crude chitin in a H2O2 aqueous solution, maintaining the water bath until decolorizing of the crude chitin iscompleted, adjusting a pH value of a decolorized chitin solution to neutral with a NaOH solution, and carrying out centrifuging so as to obtain a precipitate; and (3) adding an alkali solution into the precipitate, subjecting a mixed solution to ultrasonic treatment so as to complete a deacetylation reaction, adjusting a pH value of an obtained solution to neutral with a HCl solution, then carrying out centrifuging so as to obtain a precipitate, and carrying out drying so as to obtain a crude chitosan product. The method provided by the invention uses NaOH with a small amount and a low concentration and combines an ultrasonic process to prepare the chitosan at a room temperature; in addition, the method fully utilizes residue by-products, broadens the source of the chitosan, and is applicable to scale production of the chitosan.

Description

technical field [0001] The invention relates to the field of chitosan, in particular to a method for converting the residue after extracting active substances from ganoderma lucidum spore powder into chitosan by using ultrasound. Background technique [0002] Chitin is the second most abundant biopolymer on Earth, after cellulose, and is found in abundance in fungi, plankton, insect exoskeletons, and crustacean shells. According to an article published in Volume 524 of "Nature" in 2015, the annual biosynthesis of chitin in the world reaches 100 billion tons, but because chitin is difficult to dissolve in general solutions, it is often deacetylated converted to chitosan. Chitosan is a derivative of chitin, which can be obtained by deacetylation of chitin. Due to its good biocompatibility, degradability and non-toxicity, it is widely used in many fields such as drug carrier, tissue engineering, food and cosmetics. [0003] However, currently commercialized chitosan is almos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
CPCC08B37/00C08B37/0003
Inventor 张明暐朱礼芳陈仙乐李劲松吴子敬汪光昆
Owner ZHEJIANG UNIV
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