A method for detecting cycloserine concentration in bone tuberculosis specimens
A technology of cycloserine and bone tuberculosis, which is applied in the field of drug detection, can solve the problems of long time and increased error, and achieve the effect of improving efficiency, reducing error, and improving biological safety
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[0047] 1. Specimen Processing
[0048] Bone specimen:
[0049] 1) Immediately after taking it out, wash off the attached blood with normal saline, and dry the residual fluid and attached tissue with filter paper;
[0050] 2) After weighing, transfer the bone specimen to a 2mL externally screwed screw tube, first freeze it with liquid nitrogen, and after the liquid nitrogen evaporates, add water according to the ratio of 1g bone specimen weight to 1mL HPLC grade pure water;
[0051] 4) Grinding: Add two ceramic beads in the screw tube, grind on a homogenizer: 6m / s, 100s interval for 1min, repeat once.
[0052] 5) Place the bone homogenate at 4°C for 5 hours to extract the drug (every 1 hour, vortex at a speed of 6m / s for 20s with a homogenizer);
[0053] 6) Centrifugation: 3500g, 4°C, 10min;
[0054] 7) Collect the supernatant and subpackage in EP tubes to obtain the tissue extract (50 μl per tube), to be tested;
[0055] *The broken bone tissue obtained from trauma surgery...
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