Method of simultaneously detecting various synthetic pigments in wheaten foods via HPLC-DAD

A technology for synthesizing pigments and pasta, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of complex testing items, cumbersome processing, and many types of samples

Inactive Publication Date: 2018-03-02
昌邑市检验检测中心
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Problems solved by technology

[0003] At present, the national standard methods for detecting synthetic pigments include liquid chromatography, liquid chromatography-mass spectrometry/mass spectrometry, micro-column chromatography, etc., but most methods can only detect a few kinds of pigments at the same time, and the pretreatment process is cumbersome and the recovery rate is low. Low, especially in my country, there is no national standard for the simultaneous detection of multiple pigments. Occasionally, similar methods are reported to analyze simple matrices such as b

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  • Method of simultaneously detecting various synthetic pigments in wheaten foods via HPLC-DAD
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  • Method of simultaneously detecting various synthetic pigments in wheaten foods via HPLC-DAD

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[0042] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0043] The invention provides a method for simultaneously detecting multiple synthetic pigments in pasta by HPLC-DAD, comprising the steps of:

[0044] A. Standard working solution and sample preparation

[0045] Preparation of standard stock solution: Precisely weigh each standard substance to volume with water, and the concentration is 500 μg / mL.

[0046] Store in refrigerator at 4°C.

[0047] Preparation of standard working solution: Dilute the stock solution into a series of working solutions with concentrations of 1.0μg / mL, 2.0μg / mL, 5.0μg / mL, 10.0μg / mL, and 20.0μg / mL according to the needs.

[0048] Mixed standard ...

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Abstract

The invention relates to the technical field of detection of synthetic pigments and especially relates to a method of simultaneously detecting various synthetic pigments in wheaten foods via HPLC-DAD.The method includes the following steps: A) preparation of standard work solutions and samples; b) extraction of a sample solution; C) HPLC-DAD analysis to the solution prepared in the step B); D) calculation of content of to-be-detected substances with standard curve. In the HPLC-DAD detection method, pretreatment steps are simplified, wherein solid-phase extraction and concentration steps are avoided, thus reducing pretreatment time. Detection is carried under wavelength of 400-600 nm, so that interference due to majority of food additives and natural pigments is avoided. The method is accurate, quick and simple, has reliable quantitative results, can effectively detect ten artificially synthetic pigments in one time, and is very suitable for simultaneously treating samples in multi-batches.

Description

technical field [0001] The invention relates to the technical field of synthetic pigment detection, in particular to a method for simultaneously detecting multiple synthetic pigments in pasta by HPLC-DAD. Background technique [0002] Synthetic pigments mostly use coal tar or aromatic hydrocarbon compounds such as benzene and naphthalene as raw materials. They are synthesized through a series of organic reactions and have no nutritional value. Synthetic pigments have advantages over natural pigments such as bright colors, low cost, strong tinting strength, and relatively stable properties, so they are widely used in food processing. However, many synthetic pigments contain harmful substances, and excessive intake will cause damage to human health, and its safety issues have been increasingly valued, and countries have strict restrictions on this. Therefore, accurate quantification of synthetic pigments contained in food is of great significance to food safety, and can also ...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 王延龙姜雅红王兆全王永王向阳
Owner 昌邑市检验检测中心
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