8 results about "Hplc dad" patented technology

This invention belongs to the field of plant and component detection, specifically relating to a method for detecting anthocyanin content in different varieties of Hibiscus mutabilis and its application. The technical problem solved by this invention is to provide a method for detecting anthocyanin content in different varieties of Hibiscus mutabilis. The content detection method of this invention includes: A. Extraction and processing of Hibiscus mutabilis anthocyanin samples: mainly including optimization of flower pretreatment and extraction methods. B. Detection of the content of the extracted Hibiscus mutabilis anthocyanins using high-performance liquid chromatography (HPLC): mainly including optimization of parameters such as mobile phase type, mobile phase ratio, detection wavelength, and injection volume. This detection method can be applied to the correlation study between Hibiscus mutabilis flower color and components, for molecular breeding of Hibiscus mutabilis flower color, and for detecting the anthocyanin content of different Hibiscus mutabilis varieties to identify varieties that are difficult to distinguish.

This invention provides a method for constructing and applying a fingerprint spectrum of Chushi Weiling Decoction, belonging to the field of drug detection technology. The method involves first preparing a test solution using Chushi Weiling Decoction, then using acetonitrile (A)-0.18~0.22wt% formic acid aqueous solution as the mobile phase, and obtaining the fingerprint spectrum of Chushi Weiling Decoction through high-performance liquid chromatography (HPLC). A standard fingerprint spectrum is then established using the "Similarity Evaluation System for Chromatographic Fingerprint Spectrum of Traditional Chinese Medicine" for quality evaluation or control throughout the research, development, production, and clinical application of Chushi Weiling Decoction. This invention's method for constructing the fingerprint spectrum of Chushi Weiling Decoction enables the detection of the main medicinal components in Chushi Weiling Decoction solely through HPLC, thus providing a more comprehensive reflection of the quality status of Chushi Weiling Decoction.

The present application relates to a kind of traditional Chinese medicine composition or its preparation Shuangshenling granules characteristic map construction method and its application, the method uses high performance liquid chromatography method.Chromatographic conditions are as follows: chromatographic column: octadecylsilane bonding silica gel chromatographic column: mobile phase: with acetonitrile as mobile phase A, water or formic acid aqueous solution as mobile phase B, gradient elution is carried out.The method provided by the present application can obtain characteristic map with large information amount and high peak response value, avoids the singleness and one-sidedness of quality control, can effectively control the key quality attributes of Shuangshenling granules and other preparations, has rapid, simple, comprehensive reproducibility and stability, and is conducive to industrial industry popularization, and has important significance for Shuangshenling granules quality control standard.

This invention relates to the field of traditional Chinese medicine detection technology, specifically disclosing a method for constructing characteristic chromatograms of Sanguisorba officinalis and Sanguisorba longifolia formula granules. The method includes: preparation of a reference solution; preparation of a test solution: taking Sanguisorba officinalis formula granules or Sanguisorba longifolia formula granules, dissolving them in a solvent to obtain a test solution; determining the reference solution and test solution using high-performance liquid chromatography (HPLC) to obtain characteristic chromatograms of Sanguisorba officinalis and Sanguisorba longifolia formula granules; the HPLC chromatographic conditions are: detection wavelength: 225-235 nm for 0-16 min, 240-250 nm for 16.1-50 min; gradient elution using acetonitrile as mobile phase A and 0.15% formic acid solution as mobile phase B. This invention constructs UPLC characteristic chromatograms of Sanguisorba officinalis and Sanguisorba longifolia formula granules, with richer chromatographic peaks and better peak shapes, which can more comprehensively reflect the characteristics of Sanguisorba officinalis and Sanguisorba longifolia traditional Chinese medicine formula granules, enabling more comprehensive overall quality evaluation. The detection time is relatively shorter, and the method is stable, with good precision and reproducibility.

This invention belongs to the field of analytical chemistry detection, specifically relating to a lutetium [ 177 A method for detecting the radiochemical purity of lutetium [Lu] conjugates. The method employs high-performance liquid chromatography (HPLC) with a tandem radioactive detector. The mobile phase comprises mobile phase A and mobile phase B, wherein mobile phase A is phosphoric acid and a phosphate buffer, and mobile phase B is selected as acetonitrile and / or methanol. This invention's detection method can accurately detect lutetium [Lu]. 177 The radiochemical purity of the Lu conjugate is high, and it is stable, reliable, and highly sensitive, especially for lutetium [ 177 The quality control and research of Lu conjugate products are of great significance.

This invention belongs to the field of traditional Chinese medicine quality testing technology, specifically disclosing a method for constructing a characteristic chromatogram and determining the content of *Actinidia chinensis* root. The method for constructing the characteristic chromatogram includes the following steps: S1: Take *Actinidia chinensis* root as a reference material, add 70% methanol for ultrasonic extraction, filter, and obtain a reference solution; separately take catechin, epicatechin, proanthocyanidin B1, and proanthocyanidin B2 reference standards, add 70% methanol to prepare a mixed reference solution; S2: Take *Actinidia chinensis* root powder, add 70% methanol for ultrasonic extraction, add weight, filter, and obtain the final product; S3: Inject the samples separately, record the chromatograms, and construct the characteristic chromatogram. This invention establishes a characteristic chromatogram of *Actinidia chinensis* root using high-performance liquid chromatography (HPLC) and simultaneously determines the content of four components: proanthocyanidin B1, catechin, proanthocyanidin B2, and epicatechin, achieving comprehensive, accurate, and stable quality control of *Actinidia chinensis* root.

The present application relates to the technical field of pharmaceutical analysis, and more particularly to a modeling method for predicting the content of multiple index components of day lily, comprising the following steps: S1, collecting day lily samples from multiple producing areas, preparing the samples into powders and collecting near-infrared spectra; S2, pre-processing the data collected by near-infrared spectroscopy; S3, determining the content of components including quercetin, kaempferol, isorhamnetin and beta-sitosterol in the day lily samples by high performance liquid chromatography as reference values; S4, dividing the near-infrared spectral data into a training set and a test set. The present application is a rapid prediction model for the content of multiple index components of day lily based on near-infrared spectroscopy technology and gradient boosting decision tree algorithm, which is simple in sample pre-treatment, fast in analysis, non-destructive and environmentally friendly, and has high model prediction accuracy, providing an efficient and reliable new technical means for the quality control of day lily medicinal materials.