HPLC method based method for detecting kappa-casein typing

A casein and packaging technology, applied in the field of analysis, to achieve the effect of simple method, good repeatability and high accuracy

Pending Publication Date: 2019-05-03
北京市畜牧总站 +1
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  • HPLC method based method for detecting kappa-casein typing
  • HPLC method based method for detecting kappa-casein typing
  • HPLC method based method for detecting kappa-casein typing

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Embodiment 1

[0026] The κ-Casein from bovine milk used in this example was purchased from (Sigma-Aloririch, ≥70% (PAGE), lyophilized powder), with a certified purity of 99%.

[0027] Prepare κ-casein standard working solution with a concentration of 10g / L: Accurately weigh 10.1mg of κ-casein standard product into a 1ml stoppered graduated test tube and store at -20°C.

[0028] Preparation of working solution:

[0029] Working solution I is a mixed solution containing 0.1mol / L BisTris, 6mol / L guanidine hydrochloride, 5.37mmol / L sodium citrate, and 19.5mmol / L DTT (dithiothreitol);

[0030] Working solution II is 4.5mol / L guanidine hydrochloride solution with mobile phase A as the solution.

[0031] In this embodiment, the liquid phase system of the HPLC system is Agilent 1260, and the chromatographic column is C8 3.5 μm. 100 x 4.6 I.D. (Zorbax 300SB-C8 RP, Agilent Technologies).

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Abstract

The invention provides a high-performance liquid chromatography (HPLC) method based method for detecting kappa-casein typing in milk. A provided HPLC method can be utilized to perform typing on two variants which are AE-kappa casein and B-kappa casein in milk, so that the B type kappa casein which can enhancing cheese output can be obtained.

Description

technical field [0001] The invention belongs to the technical field of analysis, and in particular relates to a method for typing κ-casein based on an HPLC method. Background technique [0002] κ-casein is one of the most important caseins, and it is usually one of the phosphoproteins secreted by the bovine mammary gland with a small amount of phosphate groups. It not only plays a vital role in the stability of casein micelles, but also It has a certain degree of influence on the lactation performance of dairy cows, the composition of milk components and the quality of cheese. Many research results show that the bovine κ-casein gene may affect the milk yield, milk fat amount, milk protein amount, milk fat percentage and milk protein percentage of dairy cows, and has a significant impact on the coagulation characteristics of milk and cheese production. Thirteen variants of bovine κ-casein have been found, mainly three variants A, B and E, all of which are caused by non-synon...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34
Inventor 杨宇泽路永强刘林赵凤常硕吕小青常卓赵春颖陈京媛
Owner 北京市畜牧总站
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