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Method for establishing fusion-deduction fingerprint spectrum and application thereof in quality control of Yixuesheng capsule

A technology of fingerprint spectrum and establishment method, applied in the field of quality inspection and control of traditional Chinese medicine preparations, can solve the problems of easy baseline drift, amplified liquid phase system noise, unfavorable identification analysis and component quantification, etc., so as to make up for incomplete characteristic information and improve Detection accuracy, improving the effect of over-response

Active Publication Date: 2019-09-24
JILIN JINFUKANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for some Chinese medicine preparations or Chinese medicine compositions with complex components, such as Yixuesheng Capsules, which contain both animal medicines, botanical medicines, and mineral medicines, a total of 22 medicinal materials, the noise is relatively large when using the existing fusion map , can not be accurately measured, this is because the principle of this kind of fusion map is to screen the map obtained from multiple detections at all time points within the detection range to comprehensively analyze the response information, and to fuse the maximum response value to maximize the reflection of the analyte. In the process of fusion, the noise in the whole liquid phase system will not only be amplified, but also the obtained baseline is easy to drift, so that the chromatographic peak information after fusion is more, the response value of some non-main detection components is too large, and the noise increases. Baseline drift and other issues are often not conducive to subsequent differential analysis and component quantification

Method used

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  • Method for establishing fusion-deduction fingerprint spectrum and application thereof in quality control of Yixuesheng capsule
  • Method for establishing fusion-deduction fingerprint spectrum and application thereof in quality control of Yixuesheng capsule
  • Method for establishing fusion-deduction fingerprint spectrum and application thereof in quality control of Yixuesheng capsule

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The method for establishing the fusion fingerprint spectrum described in this embodiment specifically includes the following steps:

[0069] (1) Preparation of the test solution: take Yixuesheng Capsules, remove the capsule coat, and mix the contents to obtain the test product. Accurately weigh 5g of the test product, put it in a stoppered conical flask, and accurately add 15mL of methanol , dense plug, weighed, ultrasonically treated with a power of 250W and a frequency of 33KHZ for 25 minutes, taken out, allowed to cool, then weighed again, supplemented with methanol for weight loss, shaken well, filtered through a 0.22μm filter membrane, and the filtrate was obtained;

[0070] (2) Preparation of reference substance solution: Accurately weigh an appropriate amount of paeoniflorin reference substance, and use chromatographic grade methanol to prepare a solution with a concentration of 0.536 mg / mL to obtain the solution;

[0071] (3) Determination by high-performance li...

Embodiment 2

[0079] The method for establishing the fusion fingerprint spectrum described in this embodiment specifically includes the following steps:

[0080] (1) Preparation of the test solution: take Yixuesheng Capsules, remove the capsule coat, and mix the contents to obtain the test product. Accurately weigh 5g of the test product, put it in a stoppered conical flask, and accurately add 25mL of methanol , dense plug, weighed, ultrasonically treated with a power of 250W and a frequency of 33KHZ for 20 minutes, taken out, allowed to cool, and weighed again, supplemented with methanol for weight loss, shaken, filtered through a 0.22μm filter membrane, and the filtrate was obtained;

[0081] (2) Preparation of reference substance solution: Accurately weigh an appropriate amount of paeoniflorin reference substance, and use chromatographic grade methanol to prepare a solution with a concentration of 0.536 mg / mL to obtain the solution;

[0082] (3) Determination by high-performance liquid chr...

Embodiment 3

[0087] The method for establishing the fusion fingerprint spectrum described in this embodiment specifically includes the following steps:

[0088] (1) Preparation of the test solution: take Yixuesheng Capsules, remove the capsule coat, and mix the contents to obtain the test product. Accurately weigh 10 g of the test product, put it in a stoppered conical flask, and accurately add 10 mL of methanol , dense plug, weighed, ultrasonically treated with a power of 200W and a frequency of 30KHZ for 10 minutes, taken out, allowed to cool, then weighed again, supplemented with methanol for weight loss, shaken well, filtered through a 0.22μm filter membrane, and the filtrate was obtained;

[0089] (2) Preparation of reference substance solution: Accurately weigh an appropriate amount of paeoniflorin reference substance, and use chromatographic grade methanol to prepare a solution with a concentration of 0.536 mg / mL to obtain the solution;

[0090] (3) Determination by high-performance...

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Abstract

The invention relates to a method for establishing a fusion-deduction fingerprint spectrum and the application thereof in the quality control of Yixuesheng capsule. The method comprises the steps of selecting m detection wavelengths by high-performance liquid chromatography, detecting a sample to be tested at the detection wavelengths separately in order that a detection component in the sample to be tested has a large response value at at least one of the detection wavelengths; then selecting n deduction wavelengths, detecting the sample to be tested at the deduction wavelengths separately in order that the detection component in the sample to be tested has a low response value or no absorption at the deduction wavelengths and a non-detection component has a large response value at at least one of the deduction wavelengths; and then fusing the chromatographic peak information obtained by the detection wavelengths, and deducting the chromatographic peak information obtained from the deduction wavelengths to obtain a fusion-deduction fingerprint spectrum. The method obtains the most comprehensive and most accurate characteristic information of the Yixuesheng Capsule as much as possible, and solves the incomplete or inaccurate characteristic information or high noise of the traditional fingerprint spectrum technology.

Description

technical field [0001] The invention belongs to the field of quality detection and control of traditional Chinese medicine preparations, and in particular relates to a method for establishing a fusion fingerprint and its application, especially in the quality detection and control of Yixuesheng capsules. Background technique [0002] The prescription of Yixuesheng Capsules is: donkey-hide gelatin, tortoise shell glue, deer horn glue, deer blood, beef marrow, Ziheche, velvet antler, Poria cocos, astragalus (secret preparation), white peony root, angelica, Codonopsis pilosula, Rehmannia glutinosa, Atractylodes macrocephala (stir-fried bran ), making Polygonum multiflorum, jujube, fried hawthorn, fried malt, fried chicken inner gold, Anemarrhena (salt), rhubarb (wine), peanut coat. According to the Pharmacopoeia, it has the pharmacological effects of invigorating the spleen and kidney, producing blood and replenishing essence. It can be used for spleen and kidney deficiency, la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8686
Inventor 孟宪生程军刘朝辉李晓双迟晓娟张海明
Owner JILIN JINFUKANG PHARMA
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