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Establishment method of fusion fingerprint and its application in quality control of Yixuesheng capsules

A technology of fingerprint spectrum and establishment method, which is applied in the field of quality inspection and control of traditional Chinese medicine preparations, can solve the problems of amplified liquid phase system noise, baseline drift, and excessive response value, and achieves improved detection accuracy, high precision, and heavy good effect

Active Publication Date: 2021-11-05
JILIN JINFUKANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for some Chinese medicine preparations or Chinese medicine compositions with complex components, such as Yixuesheng Capsules, which contain both animal medicines, botanical medicines, and mineral medicines, a total of 22 medicinal materials, the noise is relatively large when using the existing fusion map , can not be accurately measured, this is because the principle of this kind of fusion map is to screen the map obtained from multiple detections at all time points within the detection range to comprehensively analyze the response information, and to fuse the maximum response value to maximize the reflection of the analyte. In the process of fusion, the noise in the whole liquid phase system will not only be amplified, but also the obtained baseline is easy to drift, so that the chromatographic peak information after fusion is more, the response value of some non-main detection components is too large, and the noise increases. Baseline drift and other issues are often not conducive to subsequent differential analysis and component quantification

Method used

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  • Establishment method of fusion fingerprint and its application in quality control of Yixuesheng capsules
  • Establishment method of fusion fingerprint and its application in quality control of Yixuesheng capsules
  • Establishment method of fusion fingerprint and its application in quality control of Yixuesheng capsules

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The method for establishing the fusion fingerprint spectrum described in this embodiment specifically includes the following steps:

[0069] (1) Preparation of the test solution: take Yixuesheng Capsules, remove the capsule coat, and mix the contents to obtain the test product. Accurately weigh 5g of the test product, put it in a stoppered conical flask, and accurately add 15mL of methanol , dense plug, weighed, ultrasonically treated with a power of 250W and a frequency of 33KHZ for 25 minutes, taken out, allowed to cool, then weighed again, supplemented with methanol for weight loss, shaken well, filtered through a 0.22μm filter membrane, and the filtrate was obtained;

[0070] (2) Preparation of reference substance solution: Accurately weigh an appropriate amount of paeoniflorin reference substance, and use chromatographic grade methanol to prepare a solution with a concentration of 0.536 mg / mL to obtain the solution;

[0071] (3) Determination by high-performance li...

Embodiment 2

[0079] The method for establishing the fusion fingerprint spectrum described in this embodiment specifically includes the following steps:

[0080] (1) Preparation of the test solution: take Yixuesheng Capsules, remove the capsule coat, and mix the contents to obtain the test product. Accurately weigh 5g of the test product, put it in a stoppered conical flask, and accurately add 25mL of methanol , dense plug, weighed, ultrasonically treated with a power of 250W and a frequency of 33KHZ for 20 minutes, taken out, allowed to cool, and weighed again, supplemented with methanol for weight loss, shaken, filtered through a 0.22μm filter membrane, and the filtrate was obtained;

[0081] (2) Preparation of reference substance solution: Accurately weigh an appropriate amount of paeoniflorin reference substance, and use chromatographic grade methanol to prepare a solution with a concentration of 0.536 mg / mL to obtain the solution;

[0082](3) Determination by high-performance liquid ch...

Embodiment 3

[0087] The method for establishing the fusion fingerprint spectrum described in this embodiment specifically includes the following steps:

[0088] (1) Preparation of the test solution: take Yixuesheng Capsules, remove the capsule coat, and mix the contents to obtain the test product. Accurately weigh 10 g of the test product, put it in a stoppered conical flask, and accurately add 10 mL of methanol , dense plug, weighed, ultrasonically treated with a power of 200W and a frequency of 30KHZ for 10 minutes, taken out, allowed to cool, then weighed again, supplemented with methanol for weight loss, shaken well, filtered through a 0.22μm filter membrane, and the filtrate was obtained;

[0089] (2) Preparation of reference substance solution: Accurately weigh an appropriate amount of paeoniflorin reference substance, and use chromatographic grade methanol to prepare a solution with a concentration of 0.536 mg / mL to obtain the solution;

[0090] (3) Determination by high-performance...

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Abstract

The invention relates to a method for establishing a fusion fingerprint and its application in the quality control of Yixuesheng Capsules. High-performance liquid chromatography is used to select m detection wavelengths, and the samples to be tested are respectively tested under the detection wavelengths. detection, so that the detection components in the sample to be tested have a larger response value at least one of the detection wavelengths, and then select n subtraction wavelengths, and detect the samples to be tested respectively under the subtraction wavelengths, so that the samples to be tested The detection component has a small response value or no absorption at the subtraction wavelength, and at the same time, the non-detection component has a large response value at least one of the subtraction wavelengths; then the chromatographic peak information obtained by the detection wavelength is fused, And deduct the chromatographic peak information obtained by the deducted wavelength to obtain the fusion fingerprint; obtain the most comprehensive and accurate characteristic information of Yixuesheng Capsule as much as possible, and make up for the incomplete, inaccurate or inaccurate characteristic information of traditional fingerprint technology. Noise problem.

Description

technical field [0001] The invention belongs to the field of quality detection and control of traditional Chinese medicine preparations, and in particular relates to a method for establishing a fusion fingerprint and its application, especially in the quality detection and control of Yixuesheng capsules. Background technique [0002] According to the Pharmacopoeia, Yixuesheng Capsules have the pharmacological effects of invigorating the spleen and tonifying the kidney, producing blood and replenishing essence. Chronic aplastic anemia see the above syndrome. Modern clinical studies have also reported that the drug alone or in combination can effectively treat various anemia, leukopenia, and hepatitis B and other diseases, and has good clinical use value and wide application range. But at the same time, due to the many medicinal flavors and many types of ingredients in this prescription, the current "Chinese Pharmacopoeia" has a single standard for its quality inspection, whi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8686
Inventor 孟宪生程军刘朝辉李晓双迟晓娟张海明
Owner JILIN JINFUKANG PHARMA
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