The invention discloses a dispersed solid phase extraction material and its preparation method and application, belonging to the technical field of aflatoxin detection in edible oil. The preparation method of the dispersed solid phase extraction material comprises the following steps: weighing humic acid, washing with ultrasonic waves 12‑18min, after washing with water, centrifuge to take the solid precipitate, disperse it in acetone, heat and evaporate until the acetone is completely evaporated, and obtain a residue; take the residue, put it in a Soxhlet extractor, add 180mL of cleaning solution, and heat and reflux to wash until After the reflux liquid is clear and colorless, stop heating; take out the cleaned material, dry the material at 100°C for 1.5-2.5h, cool it for 0.5-1h, and then pass through a 120-mesh sieve, take the under-sieved material, and obtain the dispersed solid phase extraction material. The preparation method of the present invention is applicable to most humic acids currently on sale, and the preparation method has simple process, is beneficial to control, and the properties of the dispersed solid-phase extraction material after cleaning and preparation are stable.