50results about How to "Realize analysis and detection" patented technology

The invention discloses a fluorescence liquid drop sorting system based on digital microfluidics and a sorting method thereof. The fluorescence liquid drop sorting system comprises a digital microfluidic chip, an integrated circuit and a fluorescence excitation and acquisition module. The digital microfluidic chip is connected with the integrated circuit; and the fluorescence excitation and acquisition module is connected with the digital microfluidic chip and the integrated circuit respectively. According to the fluorescence liquid drop sorting system based on the digital microfluidics, disclosed by the invention, generation, transportation and sorting processes of liquid drops are carried out based on an on-chip dielectric wetting principle, so that a realization mechanism of the third party does not need to be added and the miniaturization of the system is relatively easy to realize; and single liquid drops are manipulated and analyzed in the processes, and a biochemical staining and fluorescence protein marking technology is combined, so that the fluorescence liquid drop sorting system can be used for detecting and sorting single cells, secreted proteins or microorganisms, and is used for the fields of early-stage disease diagnosis and treatment and the like.

The invention relates to a gas chromatography multichannel sample introduction system which comprises a chromatographic column A, a shunt, an electronic pressure controller and at least two gas phase detectors, wherein the chromatographic column A is connected with the shunt; the gas phase detectors are mutually connected in parallel to the shunt; the electronic pressure controller is connected with the shunt; the introduced samples are conveyed to the chromatographic column A to be separated and then are conveyed to the shunt; and according to the shunt, the separated samples are divided into at least two paths to be conveyed to each path of corresponding gas phase detectors to be detected under the action of the electronic pressure controller. The invention also relates to an atmosphere detection method realized by the gas chromatography multichannel sample introduction system. The system disclosed by the invention has the advantage that multi-component compounds can be analyzed by virtue of single sample introduction, the analysis speed is high, the arrangement is flexible, and the system belongs to the technical field of gas detection.

The invention discloses a method for detecting total amount of perfluorooctane sulphonates (PFOS) in textile and leather, which sequentially comprises the following steps: solvent extraction of a sample, chemical treatment of extract liquid, purification and liquid chromatogram-mass spectroscopy. According to the invention, as PFOS in the sample are extracted by methanol with assist of ultrasonic wave, the extraction efficiency is high, the effect is obvious, and the operation is simple; a specific chemical cracking treatment is adopted so that the PFOS is decomposed into equivalent octanesulfonic acid and the measurement of perfluorooctane sulfonyl content in PFOS is realized; filler is adopted to purify the chromatographic column of silica gel, and interference of impurities in the extract liquid (oil, dye) on the liquid chromatogram-mass spectroscopy process is effectively reduced; and compared with the prior test technology, the method disclosed by the invention realizes analytical detection on the total amount of PFOS in the sample.

The invention provides a design method of amplification primers. The design method is characterized in that the amplification primers adopting hairpin structures are designed and single-stranded DNA fragments are formed at the two ends of an amplification product. The invention provides two gold nanoparticle probes; DNA sequences modified by the probes are complementary to the single-stranded DNAfragments at the two ends of a PCR product of DNA to be detected. The invention provides a quantitative PCR detection method based on gold nanoparticles. The quantitative PCR detection method comprises the following steps: (1), selecting a DNA sequence to be detected, and designing a pair of specially-structured amplification primers, wherein the pair of specially-structured amplification primersare designed to be the hairpin structures; (2), performing a PCR reaction in a system containing a DNA template to be detected, the pair of specially-structured amplification primers, Taq DNA polymerase and dNTP to obtain the PCR product; (3), preparing DNA-modified gold nanoparticle probes; (4), adding the probes into the PCR product, and hybridizing with the PCR product; (5), measuring the change of the absorbance or a dynamic light scattering signal of the gold nanoparticles. With the quantitative PCR detection method, the operation is simple, the PCR product can be rapidly detected, and the sensitivity is high.

The invention relates to the field of public safety detection and discloses a silicon-based SERS (Surface-enhanced Raman spectroscopy) chip for TNT detection and a preparation method thereof, as well as a portable platform for on-site TNT detection, built together with a 785 nm portable Raman instrument. The silicon-based chip is characterized in that a silicon wafer is modified with silver nanoparticles in situ by a hydrofluoric acid assisted silver nitrate reduction method, and then the surface of a chip is modified with a compound (for example, p-aminothiophenol) interacting with TNT, and the silicon-based chip is used for TNT capture and SERS signal enhancement. The prepared silicon-based SERS chip has excellent sensitivity, the detection limit lower than -1 pM (-45.4 fg / cm<2>), good SERS signal reproducibility and relative standard deviation (RSD) smaller than 15%. More importantly, the built portable platform for in-situ TNT detection is suitable for qualitative analysis of trace TNT in a practical environmental sample.

The invention provides a construction method of a novel photo-assisted bipolar self-energized aptamer sensor, which uses a universal meter as a direct reading strategy, and comprises the following steps: step 1, preparing a photo-anode material black titanium dioxide (BTiO2) and a photo-cathode material three-dimensional nitrogen-doped graphene hydrogel loaded cuprous oxide nanosphere (Cu2O / 3DNGH); and step 2, constructing the photo-assisted bipolar self-energized aptamer sensor for detecting sulfamethazine. The novel photo-assisted bipolar self-energized aptamer sensor constructed by the invention does not need an external power supply, the detection device supplies energy to the detection process of the sensor, and a simple universal meter is adopted as a direct reading strategy, so thatminiaturization and portability are facilitated, and field detection is realized. Meanwhile, both the anode and the cathode of the sensor are made of semiconductor materials, so that the use of bioactive components and precious metal electrodes Pt is avoided, the solar energy utilization efficiency is greatly improved, and the manufacturing cost is reduced.

The invention discloses an application of a nitrogen-doped carbon point to analysis of micro-molecular environmental pollutants, and particularly relates to the application taking the nitrogen-doped carbon point (N-CDs) as a matrix of a matrix-assisted laser desorption ionization-time of flight time mass spectrometry (MALDI-TOF MS) to the analysis of the micro-molecular environmental pollutants. By taking the nitrogen-doped carbon point as the matrix, the analysis and detection on the micro-molecular environmental pollutants with the m / z ranging from 150 to 1000 can be efficiently and rapidly realized. Suitable micro-molecule types comprise hydroxyl polycyclic aromatic hydrocarbon, nitryl polycyclic aromatic hydrocarbon, bisphenol compounds, perfluoro-acid environmental pollutants and the like. When the nitrogen-doped carbon point is used as the matrix to detect micro-molecules, background interferences of a traditional organic matrix ion peak can be effectively eliminated; an ionization agent does not need to be added and the requirements on sample treatment are reduced; the nitrogen-doped carbon point has good reproducibility and high salt resistance; and an efficient and rapid detection method is provided for the environmental pollutants and an application range of the carbon point is expanded.

The invention relates to a power-on hollow fiber membrane extractor, and belongs to the filed of the analysis of instruments. The power-on hollow fiber membrane extractor is a membrane extracting device of water solution which can quickly and effectively realize the extraction of inorganic anions in microsoluble organics by the action of an electrical field so as to convert the microsoluble organics into suitable ion chromatography for analysis, and mainly comprises a magnetic mixing device, a sample bottle, a magneton, a hollow fiber membrane, a sample bottle cap, a positive electrode, a negative electrode, a needle head, a teflon tube and a high-voltage direct current power supply. In the invention, the analysis and detection of the inorganic anions in the microsoluble organics through the ion chromatography is realized, and the application range of the ion chromatography is effectively widened; moreover, the power-on hollow fiber membrane extractor has the advantages of adopting simple device, needing low cost, and being simple and convenient to operate; the extraction balance can be reached after 5-10min; and the time of completing the extraction is greatly shortened.

The invention discloses an analysis device for integrated nucleic acid extraction and amplification detection based on a smart phone and a method thereof, which relates to the key technical field of intelligent medical instruments. According to the analysis device, the smart phone is used as a main component, the smart phone is integrated with microfluid driving, temperature control and multicolorfluorescence detection, a portable multifunctional universalization device integrating nucleic acid extraction, amplification and detection is formed, the device is combined with a micro-fluidic chip; on-chip nucleic acid extraction, isothermal amplification qualitative detection and detection of real-time fluorescent quantitative PCR (Quantitative Real-time PCR,qPCR) and multiple fluorescent PCR(Multiplex PCR) are realized, and analysis and detection of pathogen nucleic acid are realized. The analysis method comprises the steps of nucleic acid extraction, system configuration, system loading, temperature setting, image acquisition, image processing and result storage and sharing. The smart phone is used as a main component, the size is small, the cost is low, the functions are complete,the applicability is high, and the detection method is diversified.

The invention discloses a method for measuring content of an active matter in a petroleum sulfonate sample of a Daqing oil field through liquid chromatogram, the method uses a mixed solution of sodiumdodecyl benzene sulfonate and sodium 2-naphthalenesulfonate as a quantifying standard solution; the liquid chromatogram is used to analyze and detect the standard solution, a quantifying working curve is drawn; a practical sample solution of the petroleum sulfonate with certain concentration is accurately prepared, the chromatographic measurement is implemented, the corresponding concentration ofthe solution is obtained by substituting the area of the chromatographic peak into the corresponding quantifying working curve, and the result of the content result of the active matter in the practical petroleum sulfonate sample is obtained finally through the simple conversion. The method is stable in detection performance and is sensitive and fast, the analysis speed is high, the repeatabilityof the test result is good, the quantitative error is small, the deviation from the test result obtained through an extraction method is about 1%, and the requirements on tracking and monitoring thecontent of the active matter in the practical petroleum sulfonate sample used for chemical flooding of the oil field as well as the content change are met completely.

The invention provides a smoking machine with a constant flow velocity. The smoking machine comprises a cigarette clamp holder, a total particulate matter catcher, a reversing valve, a gas flow detection control device, a vacuum pump and a mainstream smoke collecting opening, wherein the cigarette clamp holder is arranged on an upper panel; the total particulate matter catcher is connected onto a mainstream smoke pipeline at the back end of clamp holder; an organic glass cover used for collecting sidestream smoke is mounted on the upper panel; a sidestream smoke outlet is formed in the upper panel positioned in the glass cover; a sidestream smoke filter screen is additionally arranged at the sidestream smoke outlet, and the sidestream smoke outlet is communicated with a sidestream smoke blower and a sidestream smoke collecting opening; the total particulate matter catcher is mounted on the upper panel; the reversing valve, the gas flow detection control device and the vacuum pump are arranged on a bottom plate; a cleaning gas circuit interface is arranged on the reversing valve. The smoking machine with the constant flow velocity, provided by the invention, has the advantages that smoking flow quantity of a cigarette can be stabilized, the setting and control of the constant flow velocity can be achieved, a smoke passage can be cleaned timely, influences on experimental results are reduced greatly, the mainstream smoke and sidestream smoke can be collected in an expanded manner during cigarette burning, and analysis and detection to compositions of the mainstream smoke and the sidestream smoke can be achieved.

The invention discloses a production method and application of a needle-point graphene electrochemical electrode. The production method includes the steps of transferring a graphene film synthesized by chemical vapor deposition to the surface of a micro-nano glass needle point, and using liquid conductive silver glue to connect the graphene and a lead to obtain a needle-point graphene electrode; using cobalt nitrate aqueous solution as electrolyte to deposit cobalt hydroxide on the surface of the needle-point graphene electrode by electrochemical deposition, and heating in a muffle furnace at 300-500 DEG C for 2-4 hours to obtain the needle-point graphene electrochemical electrode functionally modified by tricobalt tetroxide. The needle-point graphene electrochemical electrode can be used to detect micro-droplet samples. After surface modification by tricobalt tetroxide, a high-sensitivity electrochemical biosensor available for enzyme-free detection of glucose is obtained. In addition, the needle-point graphene electrochemical electrode can be used to puncture an active cell to detect intracellular metabolic process in real time.

The invention discloses an extra-high flux unicellular nucleic acid molecule real-time fluorescent quantitation analysis integrated quick detecting system. The extra-high flux unicellular nucleic acidmolecule real-time fluorescent quantitation analysis integrated quick detecting system comprises a micro-fluidic chip, an automatic sample adding device, a temperature control thermocirculator, a fluorescent imaging system and a data storage and analysis system, wherein the automatic sample adding device is provided with freedom degrees in an X axis, a Y axis and a Z axis and is used for automatically adding samples and reagents to the micro-fluidic chip; and the data storage and analysis system is used for analyzing fluorescence signals of the samples which are collected, identifying positive samples and drawing out a real-time fluorescent quantitation analytical curve of the positive samples. The extra-high flux unicellular nucleic acid molecule real-time fluorescent quantitation analysis integrated quick detecting system integrates the micro-fluidic chip, the automatic sample adding device, the temperature control thermocirculator, the fluorescent imaging system and the data storage and analysis system, can realize automatic detection treatment on the samples, can realize unicellular catching nucleic acid amplification in hundreds and millions, and can realize real-time fluorescent quantitation curve analysis.

The invention relates to a mass spectrum ion source device. The mass spectrum ion source device comprises a point electrode, an insulating medium, a power supply, an instrument interface and a gas conduct; the insulating medium is located between the point electrode and the instrument interface; a tip part of the point electrode is contacted with the insulating medium, and the other end of the point electrode is connected with the power supply; the instrument interface is grounded and connected with the other end of the power supply; a strong electric field is formed between the point electrode and the instrument interface; discharge gases are filled between the insulating medium and the instrument interface through the gas conduit, and a plasma beam is formed under the action of the strong electric field; and the plasma beam interacts with an object to be measured on the surface of the insulating medium to ionize the object to be measured, so as to realize analysis and detection. The mass spectrum ion source device provided by the invention has the advantages of being simple in structure, low in cost, no solvent and high in flux and so on, besides, a linear electric field formed by the point electrode and the instrument interface improves the transmission efficiency of ions. Moreover, the mass spectrum ion source device can also be used for mass spectrometry imaging analysis.

The invention discloses a preparation method of an atorvastatin calcium isomer mixture and its intermediate. The invention discloses a method for determining and controlling the chiral purity of atorvastatin by allowing the mixture of atorvastatin calcium and its isomer as shown in formula 1' and the isomer mixture of materials as shown in formula 2' to perform synthesis reactions. The method comprises the following steps: (1) allowing the racemic mixture of the compound as shown in formula 2 and the compound as shown in formula 12 to perform a condensation reaction so as to obtain an isomer mixture as shown in formula 13; (2) hydrolyzing the isomer mixture as shown in formula 13 by an acid, and allowing the obtained isomer mixture as shown in formula 14 to form an isomer mixture of sodium salts as shown in formula 15; (3) mixing the isomer mixture as shown in formula 15 with calcium acetate to obtain an isomer mixture as shown in formula 1'; (4) determining the chiral content of the atorvastatin calcium as shown in the formula by a HPLC method; preparing the isomer mixture of the compound as shown in formula 2' and performing derivatization to obtain 11', allowing the compound asshown in formula 2 to perform derivatization so as to obtain a compound as shown in formula 7', and determining the chiral content of the compound as shown in formula 7' by a HPLC method so as to obtain the chirality of the compound as shown in formula 2. Therefore, the chiral purity of atorvastatin calcium can be controlled.

The invention relates to a preparation method and application of a magnetic pyrenebutyric acid fluorescent probe detecting ferric ions, and belongs to the technical field of novel functional materials and analytical chemistry. Ferroferric oxide has high magnetism, 1-pyrenebutyric acid has good fluorescent performance, when ferroferric oxide and 1-pyrenebutyric acid are composited, magnetism of ferroferric oxide can be kept, the fluorescent performance of 1-pyrenebutyric acid can be kept as well, and therefore detection and separation of ferric ions can be achieved, the detection limit used for analyzing ferric ions is 0.01 mmol / L, and the linear range ranges from 0.5 mmol / L to 300 mmol / L.

The invention discloses a preparation method of an electrochemical sensor for detecting molecular imprinting of organophosphorus pesticide residue. The method comprises the following steps of: firstly preparing a solution from the raw molecules of organophosphorus pesticide serving as imprinting molecules and a functional monomer and an electrolyte; depositing the molecular imprinting polymer on the surface of a working electrode by an electrochemical polymerization method so as to finish the preparation of a molecular imprinting sensitive film; and removing the imprinting molecules of the organophosphorus pesticide from the molecular imprinting sensitive film by a proper elution method and a proper eluent so as to finish the preparation of the electrochemical sensor. The method disclosed by the invention has the advantages of fast response, high sensitivity and good selectivity; and meanwhile, the detection method is simple and quick, has strong adaptability after being popularized and particularly strong universality in pesticide detection, and has a wide market prospect.

The invention discloses a CRISPR / Cas12a-RCA electrochemical sensor detection system and application thereof. According to the present invention, the CRISPR / Cas12a-RCA electrochemical sensor detection system can be used for the detection of Escherichia coli O157: H7, has characteristics of simple operation and low cost, can accurately distinguish Escherichia coli O157: H7 from different bacteria, and has strong detection specificity; the detection concentration range is also wide, a good linear relation is achieved within the range of 10-107 CFU.mL <-1 >, the detection sensitivity is high, the lowest detection limit is 10 CFU.mL <-1 >, and the detection result is accurate and reliable. The detection method is not only suitable for detecting escherichia coli O157: H7 in food, but also has huge application potential in detection of other pathogenic bacteria or clinical diagnosis.

The invention provides a pre-treatment method and quantitative detection method of three endogenous phytohormones with different chemical properties in a plant sample. The method comprises 1) quantitatively adding isotope internal standards of various endogenous plant hormones into a plant sample and then extracting the sample solution by a solvent, 2) removing impurities in the sample solution through a TiO2 material and a boron affiliative material to obtain a test solution of multiple endogenous phytohormones in the plant sample, and 3) acquiring response data of the test solution of multiple endogenous phytohormones in the plant sample through an analytical instrument and simultaneously and quantitatively detecting multiple endogenous phytohormones in the plant sample through an internal standard method. The method can realize enrichment and purification of three low-content endogenous phytohormones with different chemical properties in a small amount of plant tissue, realize simultaneous quantitative determination and has high accuracy.

The invention relates to an on-site rapid detection method for volatile benzaldehyde in expired gas of a human body. The on-site rapid detection method comprises the following steps of S1, preparing gold nanospheres modified by p-mercaptoaniline and carbon quantum dots modified by mercaptosuccinic acid; S2, adopting electrostatic interaction and a layer-by-layer assembly strategy to prepare a GNPs-CQDs assembly body and a GNPs-CQDs@MOFs core-shell nano material; S3, the GNPs-CQDs@MOFs core-shell nanometer material being deposited on a paper base, and a GNPs-CQDs@MOFs paper chip being obtained; S4, gas benzaldehyde in exhaled gas being extracted through the GNPs-CQDs@MOFs paper chip; and S5, detecting benzaldehyde by adopting a portable Raman spectrometer and an ultraviolet lamp in a surface enhanced Raman spectroscopy (SERS) and fluorescent double-probe mode. Compared with the prior art, the method has advantages of high analysis speed, high sensitivity, low sample consumption, wide application range, simplicity and convenience in operation and the like.

The invention provides a construction method of a photo-assisted bipolar self-energized aptamer sensor, which is used for rapidly, simply, economically and sensitively detecting lincomycin (LIN), and comprises the following steps: 1, preparing a photo-anode material nitrogen-doped graphene-loaded titanium dioxide (TiO2 / NG) through adoption of a one-step hydrothermal method, preparing a photocathode material molybdenum disulfide nanosheet loaded zinc phthalocyanine (ZnPc / MoS2) by a cosolvent method without chemical reaction; and 2, constructing the light-assisted bipolar self-energized aptamer sensor for detecting the LIN. The light-assisted bipolar self-energized adapter sensor constructed by the invention does not need an external power supply, realizes two-dimensional energy conversion from light energy and chemical energy to electric energy, and has unique advantages in the aspect of pushing miniature and convenient sensing equipment. Meanwhile, the cathode and the anode of the sensor are both made of photosensitive semiconductor materials, so that the use of biomasses such as enzymes and microorganisms and a noble metal Pt electrode is avoided, the solar energy utilization efficiency is greatly improved, and the manufacturing cost is reduced.

The invention discloses a method for conveniently and rapidly detecting odor substances including geosmin and dimethyl isoborneol in a water source, and belongs to the technical field of analytical chemistry. The method is based on the dispersion liquid-liquid microextraction technology, achieves the effective enrichment and extraction of 2-MIB and GSM in a to-be-detected water sample by utilizinga specific device and a specific treatment method for the sample, and then achieves the analysis and measurement of the content of 2-MIB and GSM in the water sample by utilizing a gas chromatograph-mass spectrometer, thereby achieving the rapid separation and detection of trace 2-MIB and GSM in the water sample. The method provided by the invention is suitable for conventional drinking water andwell water or river water in a natural environment, can realize effective enrichment, rapid separation, analysis and detection of odor substances in various water bodies, and especially realizes detection of the odor substances in the water bodies in the natural environment.

The invention provides the construction method of the high-throughput ratio type chip sensor for simultaneously detecting various porcine diarrhea coronaviruses, and the change of the color of the high-throughput ratio type electrode substrate and the Prussian blue can directly read a signal, so that the simultaneous detection of the three porcine diarrhea coronaviruses is realized. The preparation method comprises the following steps: step 1, preparing ZnIn2S4 / 3DNG; and step 2, constructing a high-throughput ratio type chip immunosensor for simultaneously detecting PEDV (porcine epidemic diarrhea virus), TGEV (transmissible gastroenteritis virus) and PDCoV (PDCoV). According to the constructed high-throughput ratio type chip immunosensor, three detection areas and corresponding signal output areas are prepared, a blank area serving as a reference is added to improve the accuracy, an antibody is introduced to serve as a recognition element to construct an immunosensing platform, and a 3D printed mold is added, so that a detected object can be separated from the signal output areas; each detection area is separated, interference is reduced, three detection objects can be specifically detected at the same time, and the detection efficiency is improved.

The invention relates to a method for preparing diisononyl phthalate molecularly imprinted microspheres. The method is a method for preparing molecularly imprinted microspheres having an identification function based on the molecular imprinting technology, by adopting diisononyl phthalate as a template molecule, methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a crosslinking agent, acetonitrile as a reaction solvent, and 2,2'-azobis(2-methylpropionitrile) as an initiator. The method is advantageous in that a target compound can be enriched in an oriented manner, thusreducing matrix interferences and increasing the pretreatment efficiency of samples; the prepared diisononyl phthalate molecularly imprinted microspheres can be adopted as a filler medium for samplepretreatment, so that a target product can be effectively enriched, and analysis and detection of micro- and trace diisononyl phthalate can be achieved.

The invention provides a sulfur hexafluoride gas mass analysis system, which can realize analysis and detection of air, carbon tetrafluoride, hexafluoroethane, octafluoropropane, mineral oil and waterin sulfur hexafluoride gas mass, and is characterized in that a multi-column combined separation and multi-detector combination technology is adopted, no interference peak exits among components, theseparation degree R is greater than or equal to 1.5, the qualitative and quantitative analysis is accurate, and the sensitivity can reach up to a ppm level.

The invention belongs to the technical field of photoelectrochemical sensors, discloses a construction method of a portable potentiometric photoelectrochemical sensor for quickly detecting chemical oxygen demand, and provides a construction method of a portable potentiometric photoelectrochemical sensor consisting of a double-electrode integrated electrode chip and a miniature voltmeter. A voltmeter is used as a direct reading strategy, and the method comprises the following steps: 1, preparing a photocatalytic material titanium dioxide composite copper sulfide TiO2 / CuS; and 2, constructing the potential type photoelectrochemical sensor for detecting the chemical oxygen demand. The novel potentiometric photoelectrochemical sensor constructed by the invention does not need an external power supply, the detection device supplies power to the detection process, and the voltmeter is used as a direct reading strategy, so that miniaturization and portability are easy, and field detection is realized. And meanwhile, the photocatalytic material is used for replacing a toxic and harmful catalyst required by traditional detection, so that the secondary influence on the environment is greatly reduced, and certain environmental friendliness is achieved.

The invention discloses an electrochemical fast sweep voltammetry method with high-order repeatability and reproducibility and analysis application thereof. A data form presented by the method has the characteristics of obvious high-order repeatability and repeatability, and data results in hundreds of cycles are highly consistent. The final concentration of TdT (0.1-190 U / mL) or PP (0-9 mM) in a mixed reaction solution is changed, an FSCV is adopted to test a sensor, the initial potential is set to be 0 V, the termination potential is set to be 1 V, the scanning speed is set to be 400 V / s, and on the basis, the fast sweep method is applied to the analysis and detection of TdT and a small-molecule inhibitor of TdT. The method has the advantages that the sensitivity is high, the specificity is strong, the analysis time is short, the response speed is high, the preparation is simple, the electrochemical signals with high-order repeatability and reproducibility can be output, and the low-concentration TdT activity analysis can be well realized; the detection limits are (1) 0.067 U / mL, (2) 0.1 U / mL, (3) 0.067 U / mL and (4) 0.1 U / mL respectively, the inhibitor detection IC50 values are 1.7 mM, 1.6 mM, 1.5 mM and 1.7 mM respectively, and the application prospect is good.

The invention provides a method for quantitatively analyzing non-volatile organic acids in tobacco essence and feed liquid, and particularly relates to a method for determining by derivatizing a tobacco additive sample by using a sulfuric acid methanol solution with a certain concentration, extracting by using dichloromethane, centrifugally separating, taking a subnatant, enabling the subnatant topass through a membrane, and selecting an ion mode by using GC / MS.

The invention discloses an atmospheric aerosol collection and ion detection device and method. The device comprises a PM2.5 cutting head, a corrosion device, an absorption liquid bottle, a first ion chromatography, a second ion chromatography, an aerosol inlet, an aerosol diluting and growing cavity and an aerosol collision collector, an air inlet connected with the PM2.5 cutting head and an air outlet connected with the aerosol inlet are formed in the front end and the rear end of the corrosion device respectively, and an absorption liquid inlet connected with the absorption liquid bottle and an absorption liquid outlet connected with the first ion chromatography are formed in the two sides of the lower end of the corrosion device respectively; the aerosol inlet is simultaneously connected with the steam nozzle, the aerosol inlet, the aerosol diluting and growing cavity, the aerosol collision collector and the second ion chromatography are sequentially connected, the aerosol diluting and growing cavity is of a two-section type, and condensation pipes are wound on the outer walls of the two ends of the aerosol diluting and growing cavity respectively. The device is simple in structure and easy and convenient to operate, and can rapidly and accurately analyze the concentration of ion components in atmospheric gaseous and particulate pollutants at the same time.