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50results about How to "Realize analysis and detection" patented technology

Fluorescence liquid drop sorting system based on digital microfluidics and sorting method thereof

The invention discloses a fluorescence liquid drop sorting system based on digital microfluidics and a sorting method thereof. The fluorescence liquid drop sorting system comprises a digital microfluidic chip, an integrated circuit and a fluorescence excitation and acquisition module. The digital microfluidic chip is connected with the integrated circuit; and the fluorescence excitation and acquisition module is connected with the digital microfluidic chip and the integrated circuit respectively. According to the fluorescence liquid drop sorting system based on the digital microfluidics, disclosed by the invention, generation, transportation and sorting processes of liquid drops are carried out based on an on-chip dielectric wetting principle, so that a realization mechanism of the third party does not need to be added and the miniaturization of the system is relatively easy to realize; and single liquid drops are manipulated and analyzed in the processes, and a biochemical staining and fluorescence protein marking technology is combined, so that the fluorescence liquid drop sorting system can be used for detecting and sorting single cells, secreted proteins or microorganisms, and is used for the fields of early-stage disease diagnosis and treatment and the like.
Owner:NANJING UNIV OF SCI & TECH

Quantitative PCR detection method based on gold nanoparticles

The invention provides a design method of amplification primers. The design method is characterized in that the amplification primers adopting hairpin structures are designed and single-stranded DNA fragments are formed at the two ends of an amplification product. The invention provides two gold nanoparticle probes; DNA sequences modified by the probes are complementary to the single-stranded DNAfragments at the two ends of a PCR product of DNA to be detected. The invention provides a quantitative PCR detection method based on gold nanoparticles. The quantitative PCR detection method comprises the following steps: (1), selecting a DNA sequence to be detected, and designing a pair of specially-structured amplification primers, wherein the pair of specially-structured amplification primersare designed to be the hairpin structures; (2), performing a PCR reaction in a system containing a DNA template to be detected, the pair of specially-structured amplification primers, Taq DNA polymerase and dNTP to obtain the PCR product; (3), preparing DNA-modified gold nanoparticle probes; (4), adding the probes into the PCR product, and hybridizing with the PCR product; (5), measuring the change of the absorbance or a dynamic light scattering signal of the gold nanoparticles. With the quantitative PCR detection method, the operation is simple, the PCR product can be rapidly detected, and the sensitivity is high.
Owner:SUN YAT SEN UNIV

Application of nitrogen-doped carbon point to analysis of micro-molecular environmental pollutants

The invention discloses an application of a nitrogen-doped carbon point to analysis of micro-molecular environmental pollutants, and particularly relates to the application taking the nitrogen-doped carbon point (N-CDs) as a matrix of a matrix-assisted laser desorption ionization-time of flight time mass spectrometry (MALDI-TOF MS) to the analysis of the micro-molecular environmental pollutants. By taking the nitrogen-doped carbon point as the matrix, the analysis and detection on the micro-molecular environmental pollutants with the m/z ranging from 150 to 1000 can be efficiently and rapidly realized. Suitable micro-molecule types comprise hydroxyl polycyclic aromatic hydrocarbon, nitryl polycyclic aromatic hydrocarbon, bisphenol compounds, perfluoro-acid environmental pollutants and the like. When the nitrogen-doped carbon point is used as the matrix to detect micro-molecules, background interferences of a traditional organic matrix ion peak can be effectively eliminated; an ionization agent does not need to be added and the requirements on sample treatment are reduced; the nitrogen-doped carbon point has good reproducibility and high salt resistance; and an efficient and rapid detection method is provided for the environmental pollutants and an application range of the carbon point is expanded.
Owner:SHANXI UNIV

Analysis device for integrated nucleic acid extraction and amplification detection based on smart phone and method thereof

The invention discloses an analysis device for integrated nucleic acid extraction and amplification detection based on a smart phone and a method thereof, which relates to the key technical field of intelligent medical instruments. According to the analysis device, the smart phone is used as a main component, the smart phone is integrated with microfluid driving, temperature control and multicolorfluorescence detection, a portable multifunctional universalization device integrating nucleic acid extraction, amplification and detection is formed, the device is combined with a micro-fluidic chip; on-chip nucleic acid extraction, isothermal amplification qualitative detection and detection of real-time fluorescent quantitative PCR (Quantitative Real-time PCR,qPCR) and multiple fluorescent PCR(Multiplex PCR) are realized, and analysis and detection of pathogen nucleic acid are realized. The analysis method comprises the steps of nucleic acid extraction, system configuration, system loading, temperature setting, image acquisition, image processing and result storage and sharing. The smart phone is used as a main component, the size is small, the cost is low, the functions are complete,the applicability is high, and the detection method is diversified.
Owner:HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI +1

Method for measuring content of active matter in petroleum sulfonate sample of Daqing oil field through liquid chromatogram

ActiveCN109444282AStable detection performanceFast analysisComponent separationChemistryDetection performance
The invention discloses a method for measuring content of an active matter in a petroleum sulfonate sample of a Daqing oil field through liquid chromatogram, the method uses a mixed solution of sodiumdodecyl benzene sulfonate and sodium 2-naphthalenesulfonate as a quantifying standard solution; the liquid chromatogram is used to analyze and detect the standard solution, a quantifying working curve is drawn; a practical sample solution of the petroleum sulfonate with certain concentration is accurately prepared, the chromatographic measurement is implemented, the corresponding concentration ofthe solution is obtained by substituting the area of the chromatographic peak into the corresponding quantifying working curve, and the result of the content result of the active matter in the practical petroleum sulfonate sample is obtained finally through the simple conversion. The method is stable in detection performance and is sensitive and fast, the analysis speed is high, the repeatabilityof the test result is good, the quantitative error is small, the deviation from the test result obtained through an extraction method is about 1%, and the requirements on tracking and monitoring thecontent of the active matter in the practical petroleum sulfonate sample used for chemical flooding of the oil field as well as the content change are met completely.
Owner:LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Smoking machine with constant flow velocity

The invention provides a smoking machine with a constant flow velocity. The smoking machine comprises a cigarette clamp holder, a total particulate matter catcher, a reversing valve, a gas flow detection control device, a vacuum pump and a mainstream smoke collecting opening, wherein the cigarette clamp holder is arranged on an upper panel; the total particulate matter catcher is connected onto a mainstream smoke pipeline at the back end of clamp holder; an organic glass cover used for collecting sidestream smoke is mounted on the upper panel; a sidestream smoke outlet is formed in the upper panel positioned in the glass cover; a sidestream smoke filter screen is additionally arranged at the sidestream smoke outlet, and the sidestream smoke outlet is communicated with a sidestream smoke blower and a sidestream smoke collecting opening; the total particulate matter catcher is mounted on the upper panel; the reversing valve, the gas flow detection control device and the vacuum pump are arranged on a bottom plate; a cleaning gas circuit interface is arranged on the reversing valve. The smoking machine with the constant flow velocity, provided by the invention, has the advantages that smoking flow quantity of a cigarette can be stabilized, the setting and control of the constant flow velocity can be achieved, a smoke passage can be cleaned timely, influences on experimental results are reduced greatly, the mainstream smoke and sidestream smoke can be collected in an expanded manner during cigarette burning, and analysis and detection to compositions of the mainstream smoke and the sidestream smoke can be achieved.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC

Extra-high flux unicellular nucleic acid molecule real-time fluorescent quantitation analysis integrated quick detecting system

The invention discloses an extra-high flux unicellular nucleic acid molecule real-time fluorescent quantitation analysis integrated quick detecting system. The extra-high flux unicellular nucleic acidmolecule real-time fluorescent quantitation analysis integrated quick detecting system comprises a micro-fluidic chip, an automatic sample adding device, a temperature control thermocirculator, a fluorescent imaging system and a data storage and analysis system, wherein the automatic sample adding device is provided with freedom degrees in an X axis, a Y axis and a Z axis and is used for automatically adding samples and reagents to the micro-fluidic chip; and the data storage and analysis system is used for analyzing fluorescence signals of the samples which are collected, identifying positive samples and drawing out a real-time fluorescent quantitation analytical curve of the positive samples. The extra-high flux unicellular nucleic acid molecule real-time fluorescent quantitation analysis integrated quick detecting system integrates the micro-fluidic chip, the automatic sample adding device, the temperature control thermocirculator, the fluorescent imaging system and the data storage and analysis system, can realize automatic detection treatment on the samples, can realize unicellular catching nucleic acid amplification in hundreds and millions, and can realize real-time fluorescent quantitation curve analysis.
Owner:SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI

Mass spectrum ion source device

The invention relates to a mass spectrum ion source device. The mass spectrum ion source device comprises a point electrode, an insulating medium, a power supply, an instrument interface and a gas conduct; the insulating medium is located between the point electrode and the instrument interface; a tip part of the point electrode is contacted with the insulating medium, and the other end of the point electrode is connected with the power supply; the instrument interface is grounded and connected with the other end of the power supply; a strong electric field is formed between the point electrode and the instrument interface; discharge gases are filled between the insulating medium and the instrument interface through the gas conduit, and a plasma beam is formed under the action of the strong electric field; and the plasma beam interacts with an object to be measured on the surface of the insulating medium to ionize the object to be measured, so as to realize analysis and detection. The mass spectrum ion source device provided by the invention has the advantages of being simple in structure, low in cost, no solvent and high in flux and so on, besides, a linear electric field formed by the point electrode and the instrument interface improves the transmission efficiency of ions. Moreover, the mass spectrum ion source device can also be used for mass spectrometry imaging analysis.
Owner:HARBIN INST OF TECH AT WEIHAI

Preparation method of atorvastatin calcium isomer mixture and its intermediate

The invention discloses a preparation method of an atorvastatin calcium isomer mixture and its intermediate. The invention discloses a method for determining and controlling the chiral purity of atorvastatin by allowing the mixture of atorvastatin calcium and its isomer as shown in formula 1' and the isomer mixture of materials as shown in formula 2' to perform synthesis reactions. The method comprises the following steps: (1) allowing the racemic mixture of the compound as shown in formula 2 and the compound as shown in formula 12 to perform a condensation reaction so as to obtain an isomer mixture as shown in formula 13; (2) hydrolyzing the isomer mixture as shown in formula 13 by an acid, and allowing the obtained isomer mixture as shown in formula 14 to form an isomer mixture of sodium salts as shown in formula 15; (3) mixing the isomer mixture as shown in formula 15 with calcium acetate to obtain an isomer mixture as shown in formula 1'; (4) determining the chiral content of the atorvastatin calcium as shown in the formula by a HPLC method; preparing the isomer mixture of the compound as shown in formula 2' and performing derivatization to obtain 11', allowing the compound asshown in formula 2 to perform derivatization so as to obtain a compound as shown in formula 7', and determining the chiral content of the compound as shown in formula 7' by a HPLC method so as to obtain the chirality of the compound as shown in formula 2. Therefore, the chiral purity of atorvastatin calcium can be controlled.
Owner:WUHAN QR PHARMA CO LTD +1

Construction method of light-assisted bipolar self-energized aptamer sensor for detecting lincomycin

The invention provides a construction method of a photo-assisted bipolar self-energized aptamer sensor, which is used for rapidly, simply, economically and sensitively detecting lincomycin (LIN), and comprises the following steps: 1, preparing a photo-anode material nitrogen-doped graphene-loaded titanium dioxide (TiO2 / NG) through adoption of a one-step hydrothermal method, preparing a photocathode material molybdenum disulfide nanosheet loaded zinc phthalocyanine (ZnPc / MoS2) by a cosolvent method without chemical reaction; and 2, constructing the light-assisted bipolar self-energized aptamer sensor for detecting the LIN. The light-assisted bipolar self-energized adapter sensor constructed by the invention does not need an external power supply, realizes two-dimensional energy conversion from light energy and chemical energy to electric energy, and has unique advantages in the aspect of pushing miniature and convenient sensing equipment. Meanwhile, the cathode and the anode of the sensor are both made of photosensitive semiconductor materials, so that the use of biomasses such as enzymes and microorganisms and a noble metal Pt electrode is avoided, the solar energy utilization efficiency is greatly improved, and the manufacturing cost is reduced.
Owner:JIANGSU UNIV

Construction method of high-throughput ratio type chip sensor for simultaneously detecting various porcine diarrhea coronaviruses

The invention provides the construction method of the high-throughput ratio type chip sensor for simultaneously detecting various porcine diarrhea coronaviruses, and the change of the color of the high-throughput ratio type electrode substrate and the Prussian blue can directly read a signal, so that the simultaneous detection of the three porcine diarrhea coronaviruses is realized. The preparation method comprises the following steps: step 1, preparing ZnIn2S4/3DNG; and step 2, constructing a high-throughput ratio type chip immunosensor for simultaneously detecting PEDV (porcine epidemic diarrhea virus), TGEV (transmissible gastroenteritis virus) and PDCoV (PDCoV). According to the constructed high-throughput ratio type chip immunosensor, three detection areas and corresponding signal output areas are prepared, a blank area serving as a reference is added to improve the accuracy, an antibody is introduced to serve as a recognition element to construct an immunosensing platform, and a 3D printed mold is added, so that a detected object can be separated from the signal output areas; each detection area is separated, interference is reduced, three detection objects can be specifically detected at the same time, and the detection efficiency is improved.
Owner:JIANGSU UNIV

Construction method of portable potentiometric photoelectrochemical sensor for quickly detecting chemical oxygen demand

The invention belongs to the technical field of photoelectrochemical sensors, discloses a construction method of a portable potentiometric photoelectrochemical sensor for quickly detecting chemical oxygen demand, and provides a construction method of a portable potentiometric photoelectrochemical sensor consisting of a double-electrode integrated electrode chip and a miniature voltmeter. A voltmeter is used as a direct reading strategy, and the method comprises the following steps: 1, preparing a photocatalytic material titanium dioxide composite copper sulfide TiO2 / CuS; and 2, constructing the potential type photoelectrochemical sensor for detecting the chemical oxygen demand. The novel potentiometric photoelectrochemical sensor constructed by the invention does not need an external power supply, the detection device supplies power to the detection process, and the voltmeter is used as a direct reading strategy, so that miniaturization and portability are easy, and field detection is realized. And meanwhile, the photocatalytic material is used for replacing a toxic and harmful catalyst required by traditional detection, so that the secondary influence on the environment is greatly reduced, and certain environmental friendliness is achieved.
Owner:JIANGSU UNIV

Electrochemical fast sweep voltammetry method with high-order repeatability and reproducibility and analysis application of electrochemical fast sweep voltammetry method

The invention discloses an electrochemical fast sweep voltammetry method with high-order repeatability and reproducibility and analysis application thereof. A data form presented by the method has the characteristics of obvious high-order repeatability and repeatability, and data results in hundreds of cycles are highly consistent. The final concentration of TdT (0.1-190 U/mL) or PP (0-9 mM) in a mixed reaction solution is changed, an FSCV is adopted to test a sensor, the initial potential is set to be 0 V, the termination potential is set to be 1 V, the scanning speed is set to be 400 V/s, and on the basis, the fast sweep method is applied to the analysis and detection of TdT and a small-molecule inhibitor of TdT. The method has the advantages that the sensitivity is high, the specificity is strong, the analysis time is short, the response speed is high, the preparation is simple, the electrochemical signals with high-order repeatability and reproducibility can be output, and the low-concentration TdT activity analysis can be well realized; the detection limits are (1) 0.067 U/mL, (2) 0.1 U/mL, (3) 0.067 U/mL and (4) 0.1 U/mL respectively, the inhibitor detection IC50 values are 1.7 mM, 1.6 mM, 1.5 mM and 1.7 mM respectively, and the application prospect is good.
Owner:NINGBO UNIV

Atmospheric aerosol collection and ion detection device and method

The invention discloses an atmospheric aerosol collection and ion detection device and method. The device comprises a PM2.5 cutting head, a corrosion device, an absorption liquid bottle, a first ion chromatography, a second ion chromatography, an aerosol inlet, an aerosol diluting and growing cavity and an aerosol collision collector, an air inlet connected with the PM2.5 cutting head and an air outlet connected with the aerosol inlet are formed in the front end and the rear end of the corrosion device respectively, and an absorption liquid inlet connected with the absorption liquid bottle and an absorption liquid outlet connected with the first ion chromatography are formed in the two sides of the lower end of the corrosion device respectively; the aerosol inlet is simultaneously connected with the steam nozzle, the aerosol inlet, the aerosol diluting and growing cavity, the aerosol collision collector and the second ion chromatography are sequentially connected, the aerosol diluting and growing cavity is of a two-section type, and condensation pipes are wound on the outer walls of the two ends of the aerosol diluting and growing cavity respectively. The device is simple in structure and easy and convenient to operate, and can rapidly and accurately analyze the concentration of ion components in atmospheric gaseous and particulate pollutants at the same time.
Owner:FUDAN UNIV
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