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Pre-treatment method and quantitative detection method of three endogenous phytohormones with different chemical properties in plant sample

A technology of plant hormones and chemical properties, applied in the field of analytical chemistry, can solve the problems of inability to realize and analyze acid plant hormones, alkaline plant hormones, etc., and achieve the effect of reducing loss, easy operation and simple method.

Inactive Publication Date: 2018-01-05
武汉绿剑可瑞信科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, for other phytohormones whose chemical properties are different from brassinosterols (BRs), it cannot be achieved with the above scheme
At present, it is still very difficult to analyze acidic phytohormones, basic phytohormones and brassinosterols simultaneously

Method used

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  • Pre-treatment method and quantitative detection method of three endogenous phytohormones with different chemical properties in plant sample
  • Pre-treatment method and quantitative detection method of three endogenous phytohormones with different chemical properties in plant sample
  • Pre-treatment method and quantitative detection method of three endogenous phytohormones with different chemical properties in plant sample

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preparation example Construction

[0041] Magnetic TiO 2 The preparation process is as follows: the ferric iron tetroxide (Fe 3 o 4 ) magnetic nanoparticles uniformly dispersed in a solution containing 100mL 0.1mol / L ammonium fluorotitanate ((NH 4 ) 2 TiF 6 ), 0.3mol / L boric acid (H 3 BO 3 ) in a polytetrafluoroethylene container; then, place the open container in a vacuum drying oven to remove the gas on the surface of the magnetic particles; then close the lid tightly, and place the container in a shaker at 35°C for 16 hours to obtain a solid product; the obtained solid product was washed with water for 5 times and then dried in an oven at 120°C; the dried solid was placed in a muffle furnace and heated at 1°C min -1 The heating rate was raised to 300°C, and kept for 2h, and then cooled to room temperature naturally, the obtained Fe 3 o 4 / TiO 2 Composite material, namely magnetic TiO 2 Material.

[0042] The preparation process of the magnetic boron affinity material is as follows: first take 4-N,...

Embodiment 1

[0044] A pretreatment method for multiple endogenous plant hormones in plant samples, the main steps are as follows:

[0045] (1) Rapeseed flower (approximately 100mg) was ground to powder in a mortar under the freezing of liquid nitrogen, transferred to a 1.5mL centrifuge tube, and accurately added 1.0ng[ 2 h 2 ]IAA, 1.0ng[ 2 h 6 ]ABA,0.4ng[ 2 h 2 ]GA1,0.4ng[ 2 h 2 ]GA3,0.4ng[ 2 h 2 ]GA4,0.4ng[ 2 h 2 ]GA12,0.4ng[ 2 h 2 ]GA24,0.4ng[ 2 h 2 ]GA53, 1.0ng[ 2 h 3 ]BL,1.0ng[ 2 h 3 ]CS, and 0.1ng of [ 2 h 6 ] iP, [ 2 h 6 ] iPR, [ 2 h 6 ] iP9G, [ 2 h 5 ]tZ,[ 2 h 5 ]t ZR,[ 2 h 5 ]tZ9G,[ 2 h 3 ]DHZ and [ 2 h 3 ] DHZR as internal standard; then add 1.0mL of acetonitrile to the centrifuge tube, vortex evenly, place in -20°C refrigerator for overnight extraction (12h), then centrifuge at 1000rpm, 0°C for 20min, take Put the supernatant into a new 1.5mL centrifuge tube, which is the sample solution;

[0046] (2) (a) remove the solvent in the sample solutio...

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Abstract

The invention provides a pre-treatment method and quantitative detection method of three endogenous phytohormones with different chemical properties in a plant sample. The method comprises 1) quantitatively adding isotope internal standards of various endogenous plant hormones into a plant sample and then extracting the sample solution by a solvent, 2) removing impurities in the sample solution through a TiO2 material and a boron affiliative material to obtain a test solution of multiple endogenous phytohormones in the plant sample, and 3) acquiring response data of the test solution of multiple endogenous phytohormones in the plant sample through an analytical instrument and simultaneously and quantitatively detecting multiple endogenous phytohormones in the plant sample through an internal standard method. The method can realize enrichment and purification of three low-content endogenous phytohormones with different chemical properties in a small amount of plant tissue, realize simultaneous quantitative determination and has high accuracy.

Description

technical field [0001] The invention relates to a pretreatment method and a quantitative detection method for three types of endogenous plant hormones with different chemical properties in plant samples, belonging to the field of analytical chemistry. Background technique [0002] Phytohormones play an extremely important role in all stages of plant growth and development. Simultaneously and accurately quantifying various phytohormones in plant tissues is helpful to understand the physiological functions and regulatory networks of phytohormones. However, due to the complex matrix, extremely low content and different chemical properties in plant extracts, it is difficult to simultaneously determine multiple phytohormones in plant samples. [0003] Most of the analytes targeted by the reported pretreatment methods for various phytohormones in plants are one or more of acidic phytohormones and basic phytohormones. The existing patents ZL 2012 1 0486507.3 and ZL 2013 10131422....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34G01N1/40G01N30/02
Inventor 冯钰锜蔡保东罗晓彤
Owner 武汉绿剑可瑞信科技有限公司
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