Pre-treatment method and quantitative detection method of three endogenous phytohormones with different chemical properties in plant sample

A technology of plant hormones and chemical properties, applied in the field of analytical chemistry, can solve the problems of inability to realize and analyze acid plant hormones, alkaline plant hormones, etc., and achieve the effect of reducing loss, easy operation and simple method.
CN107543746AInactive Publication Date: 2018-01-05武汉绿剑可瑞信科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
武汉绿剑可瑞信科技有限公司
Publication Date
2018-01-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a pre-treatment method and quantitative detection method of three endogenous phytohormones with different chemical properties in a plant sample. The method comprises 1) quantitatively adding isotope internal standards of various endogenous plant hormones into a plant sample and then extracting the sample solution by a solvent, 2) removing impurities in the sample solution through a TiO2 material and a boron affiliative material to obtain a test solution of multiple endogenous phytohormones in the plant sample, and 3) acquiring response data of the test solution of multiple endogenous phytohormones in the plant sample through an analytical instrument and simultaneously and quantitatively detecting multiple endogenous phytohormones in the plant sample through an internal standard method. The method can realize enrichment and purification of three low-content endogenous phytohormones with different chemical properties in a small amount of plant tissue, realize simultaneous quantitative determination and has high accuracy.
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Description

technical field

[0001] The invention relates to a pretreatment method and a quantitative detection method for three types of endogenous plant hormones with different chemical properties in plant samples, belonging to the field of analytical chemistry. Background technique

[0002] Phytohormones play an extremely important role in all stages of plant growth and development. Simultaneously and accurately quantifying various phytohormones in plant tissues is helpful to understand the physiological functions and regulatory networks of phytohormones. However, due to the complex matrix, extremely low content and different chemical properties in plant extracts, it is difficult to simultaneously determine multiple phytohormones in plant samples.

[0003] Most of the analytes targeted by the reported pretreatment methods for various phytohormones in plants are one or more of acidic phytohormones and basic phytohormones. The existing patents ZL 2012 1 0486507.3 and ZL 2013 10131422....

Claims

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