47 results about "Geosmin" patented technology

The invention discloses a system and a method for removing natural organic substances and odor-induced substances in micro polluted water, which belong to the fields of water treatment processes and equipment. The system comprises an aeration biological filter tower, an ultra-filtration membrane assembly and a regulating tank, wherein the bottom of the aeration biological filter tower is connected with the bottom of the regulating tank through a pipeline; the regulating tank is provided with two water outlet passages, namely a direct water outlet passage of the regulating tank and a water passage for connecting the regulating tank and the ultra-filtration membrane assembly; the water outlet of the ultra-filtration membrane assembly is connected with the water inlet of a clean water tank through a water outlet pump. The invention also discloses the method for removing the natural organic substances and the odor-induced substances in the micro polluted water by using the system. The treatment process can simultaneously remove the natural organic substances and 2-methyl isoborneol and geosmin serving as the odor-induced substances in the water, the quality of the discharged water is good, the adding amount of a disinfectant is obviously reduced, and the generation of disinfection byproducts is reduced.

The invention belongs to the technical field of natural environment water quality monitoring and discloses a method for determining eight kinds of common odorous substances in water simultaneously through use of a method of combining purge-and-trap and gas chromatography/mass spectrometry. The method can be adopted to determine eight kinds of common odorous substances such as dimethyl sulfide (DMS), dimethyl trisulfide (DMTS), 2-isopropyl-3-methoxy pyrazine (IPMP), 2-isobutyl-3-methoxy pyrazine (IBMP), 2-methyl isobornyl (MIB), beta-cyclocitral, geosmin (GSM) and beta-ionone in water simultaneously. Any report on the method for determining the eight kinds of substances in one step is not present yet. The method disclosed by the invention fills the gap and can be used for quantitatively analyzing the eight kinds of odorous substances in water in one step, so that the detection efficiency is greatly increased.

The invention discloses a method for determining earthy-smell substances in fish flesh, which comprises the following steps: taking fish flesh prepared by taking a cis-decahydro-1-naphthol solution asan internal standard as a sample, extracting geosmin and 2-methylisoborneol in the sample by adopting a headspace solid-phase microextraction method, and performing a determination process by adopting a gas chromatography-mass spectrometry method. A headspace solid-phase microextraction method is adopted to extract earthy-smell substances in the fish flesh, cis-decahydro-1-naphthol is taken as aninternal standard, and qualitative and quantitative determination is carried out by a gas chromatography-mass spectrometry method, so that the detection method of the geosmin and the 2-methylisoborneol in the fish flesh is established, and a detection method basis is provided for removing peculiar-smell substances in cultured aquatic products.

The invention aims at overcoming the defects of the existing sample detection technology, and particularly relates to a method for determining geosmin and 2-methylisoborneol in sediments, which comprises the following steps of sampling, headspace solid phase micro-extraction and GC-MS analysis. According to the method, the extraction effects of different types of extraction heads are compared, theextraction conditions such as the extraction time, the extraction temperature and the like are optimized, the headspace solid phase micro-extraction/gas chromatography/mass spectrometry method is established to simultaneously determine the geosmin and the 2-methylisoborneol in the sediment, and the method has characteristics of sensitivity, rapidness, environmental protection and the like, can accurately quantify the odor substances in the sediment, and can meet the detection requirements of the actual sample.

The invention discloses a novel bacillus amyloliquefaciens, wherein a bacillus amyloliquefaciens is separated from high-temperature white spirit brewing yeast, and collected in the China Centre for Type Culture Collection (CCTCC) on August 12, 2013, and the collection number is CCTCC NO: M2013368; and the generation of geosmin smell in white spirit can be controlled by adding a certain amount of bacillus amyloliquefaciens during the white spirit brewing process. The strain is capable of synthesising active substances such as antimicrobial lipopeptides, suppressing the growth of geosmin-generating streptomycete and the synthesis of geosmin during the white spirit brewing process, and fundamentally controlling the geosmin smell in white spirits of a highly flavoured type, a faint flavour type, a soy sauce flavour type and the like.

The invention discloses a strain for generating geosmin and a screening method thereof, belonging to the technical field of biological engineering. The invention discloses a method for screening and identifying relevant strains of aromatic substances generated by microbial metabolism in white wine in a yeast preparing process by combining a smelling means and taking flavor as guidance. By adopting the method, four strains for generating geosmin are obtained by screening in yeast produced in a faint scent white wine factory, and are respectively named as LBG-FXJ, FJ-HX, QC-1 and QC-2; the four strains are streptomyces according to the comparision result of bacterial colony, cell morphology and physiological biochemical features as well as a 16SrRNA gene sequence; a large amount of geosmin substances can be accumulated by the four strains in a patato-glucose culture medium, thus solving the problem of the source of the geosmin substances in the faint scent white wine; the four strains of streptomyces obtained by screening can be used as mode-type bacteria; and the method is used for detecting the strain for generating the geosmin during white wine production and researching the generated mechanism.

The invention discloses novel bacillus pumilus which is separated from high-temperature spirit brewing yeast. The bacillus pumilus was preserved at the China Center For Type Culture Collection (CCTCC) in August 12th, 2013, and the preserving serial number is CCTCC NO: M2013372. A certain amount of bacillus pumilus is added during white spirit brewing, so that generating of the geosmin in the white spirit is controlled. The bacillus pumilus can synthesize antibiosis lipopeptid active substances to inhibit growing of geosmin streptomycete generated during white spirit brewing and synthesis of the geosmin, and the geosmin in white spirits with different flavors of a thick type, a thin type and a paste type can be controlled fundamentally.

The invention provides a synthesis method of geosmin. The method is characterized in that 2-methyl cyclohexanone used as a raw material for an initial reaction firstly is in ammoniation reaction with (S)-Alpha-phenylethylamine to produce an enimic compound; the enimic compound is in substitution reactio with olefine ketone to produce an enimic compound substituted by pentyl ketone; afterwards, the enimic compound is converted to a cyclohexanone derivative under an acidic condition; Alpha, Beta unsaturated derivatives are produced through intramolecular cyclization under an alkaline condition; and the enimic compound is subject to reactions of epoxidation reaction, reduction, dehydroxylation, and the like to produce the final target compound, namely the geosmin. The synthesis method of the geosmin, which is provided by the invention, has the advantages that the efficiency is high, the operation is simple, convenient and safe, the costs of raw materials used for the reactions are low, the use amount of solvents is small, and the preparation cost can be reduced on the premise that the product purity and the yield are guaranteed, so that the synthesis method is very suitable for hundred-milligram scale batch production.

The invention discloses a kineococcus cavernae sp. and an application thereof. The strain is named kineococcus cavernae sp. SYSUZL-1, and is preserved in the China Center For Type Culture Collection (CCTCC) on December 9, 2016, and the preservation number is M2016738. A microbiological characteristic analysis and a 16SrRNA gene sequence analysis indicate that the strain is a novel bacterium. The strain can produce benzaldehyde and volatile compounds including geosmin, elemene, elemol and Alpha-eudesmol, and after fermentation, a tobacco flavor can be prepared. The obtained flavor has unique fragrance, can enhance a cigarette style, and can enrich cigarette fragrance, so that the fragrance is fuller, moreover, the flavor has antibacterial and antifungal activity, and the effect is stable and persistent. In addition, the strain also has a tremendous potential in the development of novel, highly effective, nontoxic and natural medicines.

The invention discloses a method for conveniently and rapidly detecting odor substances including geosmin and dimethyl isoborneol in a water source, and belongs to the technical field of analytical chemistry. The method is based on the dispersion liquid-liquid microextraction technology, achieves the effective enrichment and extraction of 2-MIB and GSM in a to-be-detected water sample by utilizinga specific device and a specific treatment method for the sample, and then achieves the analysis and measurement of the content of 2-MIB and GSM in the water sample by utilizing a gas chromatograph-mass spectrometer, thereby achieving the rapid separation and detection of trace 2-MIB and GSM in the water sample. The method provided by the invention is suitable for conventional drinking water andwell water or river water in a natural environment, can realize effective enrichment, rapid separation, analysis and detection of odor substances in various water bodies, and especially realizes detection of the odor substances in the water bodies in the natural environment.

A method of eliminating the off-flavor of soy beans, wherein the off-flavor of the soy beans is not related to geosmin, the method comprising:

providing soy beans in a vacuum tumbler equipped with lifting ribs;

tumbling the soy beans in a temperature range from about 0.5° C. to about 27° C. in an alternating environment of a vacuum and an acidic solution that is not hypotonic and does not comprise sodium, wherein the lifting of the ribs lift the soy beans completely out of the acidic solution when the soy beans are in the vacuum environment;

and

removing at least a portion of the moisture from the soy beans; wherein said process produces soy beans that have no-off flavor are free of trans fats and retain good flavor is described along with related products.

The invention discloses a standard substance for quantitatively detecting bacterial strains producing geosmin and a preparation and detection method. The detection method comprises the following stepsthat step one, a recombinant plasmid is used as a standard substance template, PCR amplification is carried out by real-time quantitative PCR, a standard curve corresponding to concentration of the standard substance and a critical recurring number is established, the recombinant plasmid is a recombinant plasmid comprising a GSY gene or a recombinant cell comprising the GSY gene, and a sequence of the GSY gene is composed of 125 deoxyribonucleotides and is a sequence in a sequence table SEQ ID NO.1; and step two, a sample to be tested is used for replacing the standard substance in the step one, real-time quantitative PC amplification is carried out, according to the critical recurring number and the standard curve of an amplified result, a copy number of the GSY gene in the sample to betested is obtained, and namely, the number of the bacterial strains producing the geosmin in the sample to be tested is obtained. According to the standard substance for quantitatively detecting the bacterial strains producing the geosmin and the preparation and detection method, rapid and quantitative detection of the bacterial strains producing the geosmin in a water environment sample can be realized.

The invention discloses a method for degrading odor substances in water based on a disinfection technology, which comprises the following steps: adding a disinfectant solution into water containing smelly substances, then carrying out ultraviolet irradiation, and after a period of reaction, sampling and analyzing to obtain the degradation effect of the smelly substances in water. The odor substance is 2-methylisoborneol or geosmin, the disinfectant is a chloramine solution, and the irradiation intensity of ultraviolet irradiation is (1.42-4.26) * 10<-6 > Einstein.L<-1 >. S<-1 >. The method is easy to operate, remarkable in effect, safe to use and environmentally friendly. A relatively good degradation effect can be achieved in a short time under a normal-temperature condition, and a good degradation effect is achieved under neutral, acidic or alkaline conditions. According to the technology, other equipment and medicaments do not need to be additionally added, the operation is simple, the efficient degradation of the odor substances in the water can be realized, and meanwhile, the synergism of ultraviolet and chloramine combined disinfection can be exerted.

The invention provides a heavy metal passivation and geosmin inhibition agent in an aquaculture process, and a preparation method thereof, and belongs to the technical field of aquaculture quality evaluation. The preparation method of the agent comprises the following steps: firstly, selecting apricot shells with wide sources as a raw material, raising the temperature from room temperature to a target temperature of 550 DEG C, then keeping the temperature for 2 h, collecting crude pyroligneous liquor at the temperature of 550 DEG C through a destructive distillation and condensation process, then standing the crude pyroligneous liquor for one month by utilizing the instability of part of components in the pyroligneous liquor and easy precipitation caused by oxidation or polymerization andthe like, and discarding lower-layer tar and other components to obtain refined pyroligneous liquor which is used as a heavy metal passivation and geosmin inhibition agent in the aquaculture process.The passivation efficiency on copper, zinc and lead in the aquaculture process is high, the passivation range is wide, and a good passivation effect on various heavy metals is achieved; and fishy smell substances can be removed, and the market application prospect is wide.

The invention discloses a method for inhibiting geosmin in yeast for making hard liquor, and belongs to the technical field of microorganisms. According to the invention, the microbial agent containing Bacillus licheniformis, Bacillus pumilus, Saccharomyces cerevisiae and Lactobacillus brevis is added into the yeast for making hard liquor, so that the microorganism capable of producing the geosminin the yeast for making hard liquor is inhibited, the content of the geosmin in the yeast for making hard liquor is greatly reduced, the content of the geosmin in the yeast for making hard liquor obtained by adding the microbial agent of the invention is reduced to about 3.4 mug/kg, compared with that of the yeast for making hard liquor obtained by not adding the microbial agent of the invention,the average decrease is about 70% ,and decrease range is obvious.

The invention discloses a thermoactinomyces thalpoillus strain and application thereof. The thermoactinomyces thalpoillus strain is morphologically characterized in that the strain is cultured on an ISP2 culture medium for 3 days at 50 DEG C, wherein round protruding non-transparent colonies 6-7mm in diameter are dry, dense and less prone to being poked, have rugae and white spores and are free from soluble pigment, medium mycelia are faint yellow, and aerial mycelia are white; under observation of a scanning electron microscope, single spores are formed on the mycelia and insert on the same without stems or directly, and single side of every spore grows on two sides of the corresponding mycelium; the thermoactinomyces thalpoillus strain is waxy in texture, non-transparent and smooth in colony edge. The thermoactinomyces thalpoillus strain is capable of yielding geosmin substances.

A pretreatment method for detecting geosmin substances in aquatic products comprises the following steps: taking edible tissues of a water product, cutting the edible tissues into pieces, putting the pieces into a centrifuge tube with a plug, then adding papain and double distilled water, carrying out vortex treatment, uniformly mixing, and standing in a water bath; then adding n-hexane, whirling, uniformly mixing, placing in an ultrasonic bath, centrifuging, taking the supernatant, repeating the steps in sequence, merging the two supernatants, and transferring into a new centrifuge tube with a plug; adding a purification extraction bag into the centrifugal tube with the plug; carrying out vortex and centrifugation on the centrifugal tube, taking supernate, transferring the supernate to a new centrifugal tube, and sealing a cover for later use; using a solid-phase extraction column, using normal hexane for leaching, usingethyl acetate for elution, and after eluent passes through an organic phase filter membrane, performing GC-MS automatic sample injection and detection. According to the method, the pretreatment time and the use cost of consumables are greatly saved, the detection recovery rate of the geosmin substances in the aquatic products can be increased to about 80%, the treatment method is simple and can be operated in batches, and the applicability of the method is also enhanced in the aspect of quality control.

The invention belongs to the technical field of aquaculture and food monitoring, discloses a method for determining geosmin and dimethylisoborneol in a fish body based on a rapid pretreatment technology, and establishes a detection method for determining GSM and 2MIB in fish tissues through dispersive solid phase extraction-gas chromatography-mass spectrometry. The method comprises the following steps: extracting a sample with an acetonitrile solution, and performing salting-out assisted extraction; after purification, under a gradient heating condition, subjecting a target analyte to qualitative analysis by comparing retention time and ion pair information in a selected ion monitoring mode, and performing quantification by an internal standard method. The method has the advantages of small required sample amount and reagent amount, high analysis speed, high sensitivity and good reproducibility. The method can meet the large-batch detection requirements of GSM and 2MIB in fish tissue samples, and provides a rapid detection technical guarantee for evaluating the occurrence condition of earthy smell substances in fish tissues and exploring the reduction and removal of typical earthy smell substances in fish bodies.