57 results about "Olfactory receptor" patented technology

Embodiments of the present application provide a method, device, medium, equipment and product for classifying wine samples, relating to the technical field of artificial intelligence. The method comprises: contacting odor molecules of a wine sample with at least one recombinant cell expressing an olfactory receptor and a reporter protein, the reporter protein generating a detectable signal after the receptor binds to the odor molecules; obtaining time series data of the signal, calculating the maximum value and baseline value thereof, and determining a response characteristic value of each olfactory receptor according to the same; and inputting the characteristic value into a trained machine learning model to obtain a wine sample classification result. The present method accurately extracts a stable characteristic value of the response of an olfactory receptor to a wine sample, thereby eliminating interference caused by initial value fluctuations and improving the accuracy of model classification.

The invention provides methods for associating volatile compounds or mixtures of volatile compounds with combinatorial response patterns of Olfactory Receptors (OlfRs), a biosensor array comprising a plurality of biosensors, the use of said biosensor and of the method of the invention to screen for diseases, a molecule identified by a method of the invention as well as the use se of said molecule to modify the behavior of an insect.

The present invention addresses the problem of providing a cancer inspection technology in which an olfactory receptor is used. The problem is solved by a cancer test agent containing an insect olfactory receptor protein B' which demonstrates reactivity to multiple types of cancer with the same reactivity direction.

The invention discloses a method for analyzing aroma components of cooking wine based on molecular docking and an odor activity value and application. According to the method, cooking wine aroma is taken as a research object, volatile substances are detected by gas chromatography-mass spectrometry (GC-MS) and headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS), and by combining with odor activity value (OAV) analysis, the combining capacity of aroma molecules and olfactory receptors is simulated through molecular docking, characteristic aroma compounds are screened, and the aroma of the cooking wine is detected. And finally, constructing a recombinant aroma model according to the characteristic aroma compounds, carrying out sensory evaluation, and evaluating the aroma similarity of the recombinant aroma model and the natural cooking wine sample to determine the characteristic aroma components of the cooking wine. The method can be used for analyzing aroma substances of cooking wine and performing standardized flavor management.

To provide a method for screening an olfactory receptor protein or its combination useful in cancer examination.SOLUTION: A method for screening an olfactory receptor protein or a combination thereof, comprising testing the reactivity of a test olfactory receptor protein to cancer, and selecting at least one selected from the group consisting of (1) a combination of the olfactory receptor protein A and the olfactory receptor protein C, (2) a combination of the olfactory receptor protein A ' and the olfactory receptor protein B, and (3) the olfactory receptor protein B '.SELECTED DRAWING: None

The present application belongs to the technical field of fruit fly prevention and control, and discloses a disorienting agent, a preparation method and application thereof. The disorienting agent contains rosemary extract and orange peel essential oil. The rosemary extract and orange peel essential oil contain terpenes and other compounds (such as 1,8-cineole in rosemary), which can compete with natural signal molecules to bind to odor binding proteins or olfactory receptors, which can cause fruit flies to fail to activate the correct nerve signal or directly block the signal transduction pathway. The orange peel essential oil in the disorienting agent has a strong smell, which can strongly cover or mask the weak smell signal emitted by the fruit, forming an "olfactory masking" effect, so that the fruit fly cannot find the fruit. Moreover, compared with sex pheromones as disorienting agents, which can only act on male fruit flies, the disorienting agent of the present application can interfere with the olfactory receptors of adult fruit flies to make them unable to find the target, so that they cannot accurately locate the oviposition site and harm the fruit. The disorienting agent is effective for both female and male fruit flies.

The invention discloses application of 6-methylcoumarin in preparation of a medicine for treating prostatic cancer, and relates to the technical field of medicines. The technical problem to be solved is to provide application of 6-methylcoumarin in preparation of drugs, health care products and functional food for treating prostatic cancer. The key point of the technical scheme is as follows: 6-methylcoumarin is used as an efficient ligand of an olfactory receptor OR51E2 to regulate prostate cancer cells so as to treat prostate cancer. In the prostate cancer cells LNCaP highly expressed by OR51E2, the 6-methylcoumarin can significantly inhibit cell proliferation, the inhibition effect of the 6-methylcoumarin is positively correlated with the action concentration of the 6-methylcoumarin, a new molecular target and a technical path are provided for prostate cancer treatment, the adopted 6-methylcoumarin has good chemical stability and application safety, and the 6-methylcoumarin has good application prospects. The potential of further drug development and transformation application is realized.

This invention provides a simple method for evaluating whether or not a peptide binds to isovaleric acid. [Solution] A method for evaluating the binding affinity to isovaleric acid, comprising a preparation step of preparing a fluorescently labeled compound in which a fluorescent group is bonded to the α-carbon atom of isovaleric acid, and a contact step of contacting the fluorescently labeled compound with a peptide fragment of an olfactory receptor.

The application discloses a multi-parameter integrated bionic smell sensing chip and a preparation method and application thereof, and has the characteristics of high flux, small size, high integration and low energy consumption by integrating tens of human olfactory receptor proteins and field effect transistors on the same chip by combining MEMS technology and bionic sensing technology. When gas molecules are combined with the olfactory receptor proteins on the chip, the protein conformation changes, the gate current of the field effect transistor is changed, the current signal is normalized, the response spectrum of different gas molecules is constructed to form a smell database, the cross-sensitivity of the olfactory receptor proteins is utilized, and a pattern recognition algorithm is combined to realize high-dimensional detection and accurate identification of a large number of different smells. The smell sensing chip has wide application prospects in the fields of air quality monitoring, food safety and hazardous product detection.

Provided in the present invention are a method for preparing a compound and the use thereof as an olfactory receptor OLFR110 agonist. The compound of the present invention is capable of activating OLFR110 in vitro, and binding to OLFR110. The results of animal experiments show that the compound can significantly reduce the body weight of mice and improve blood glucose homeostasis. The compound of the present invention or a pharmaceutically acceptable salt or solvate thereof can be used as a potential pharmaceutical lead compound for treating obesity and diabetes.

This invention discloses a nanopore sensor based on insect olfactory receptor proteins, its construction method, and its applications, belonging to the field of biosensors. This invention obtains the nanopore sensor by integrating insect olfactory receptor proteins as sensitive elements into a nanopore measurement system. By measuring the nanopore current signal, the prepared sensor is applied to the detection of target odor molecules. This invention utilizes the recognition ability of insect olfactory receptor proteins for odor molecules and the single-molecule level detection capability of nanopores, achieving sensitive, rapid, and direct detection of odor substances without the need for expensive, large-scale experimental equipment and complex experimental procedures.

This invention discloses a method for screening odor inhibitors based on olfactory receptors and intermolecular interactions in the field of deodorization technology. The method includes: using an olfactory receptor database, multi-level screening or machine learning to predict olfactory receptors that a target odor pollutant can bind to or activate; multi-level screening or machine learning to predict potential inhibitors of the olfactory receptors; calculating the binding energy between the olfactory receptors and the target odor pollutant, and the screened or predicted inhibitors; preferentially selecting potential inhibitors with binding energies lower than those of the target odor pollutant; and using an electronic nose to test the odor-reducing effect of the potential inhibitors on the target odor pollutant. This invention proposes a screening method for odor inhibitors from the perspective of olfactory mechanisms. By utilizing the competitive and inhibitory effects of substances on olfactory receptors, it weakens the olfactory response of the target odor pollutant, thereby achieving the effect of odor suppression. Compared with manual olfactory screening methods, this method can effectively improve the development efficiency of deodorants and reduce workload, cost, and health risks.

The invention discloses a tomato leaf miner behavior regulating agent and a screening method and application thereof, and relates to the technical field of agricultural pest control, and the active ingredients of the behavior regulating agent are any one or more of limonene, E-beta-caryophyllene and terpinene. The screening method comprises the following steps: screening volatile matters with high affinity with a tomato leaf miner olfaction receptor from a tomato volatile matter library through a molecular docking technology, and carrying out biological activity verification in combination with an electroantennal potential experiment and a Y-type olfactometer behavior experiment, so as to accurately obtain the component with remarkable attraction activity. The behavior regulating agent can be used for preparing an attractant and is applied to green prevention and control of tomato leaf miners. According to the invention, efficient screening from a molecular mechanism to a behavior effect is realized, and a new product and technical support are provided for targeted prevention and control.

The invention discloses perfume lemon compound essential oil as well as a preparation method and application thereof. The perfume lemon compound essential oil comprises an active component A and an active component B, the active component A comprises perfume lemon essential oil, rosemary oil, tea tree oil and cedar oil; the active ingredient B comprises at least one of thyme oil, schizonepeta oil, lemongrass oil, peppermint oil and eucalyptus oil. According to the prepared perfume lemon compound essential oil, the perfume lemon essential oil is creatively used as a main component and forms molecular-level cooperation with other plant essential oil in a specific proportion, scientific component selection and proportion optimization are achieved, the perfume lemon essential oil and other plant essential oil jointly act on olfactory receptors of mosquitoes, a strong repelling signal is generated, and the mosquito repelling effect is achieved. The key technical problems that an existing plant mosquito repellent product is insufficient in mosquito repellent durability, high in skin irritation, poor in formula stability and the like are successfully solved, and the plant mosquito repellent has good application prospects.

Described herein are olfactory receptor proteins having a modified C-terminal domain comprising a specific amino acid sequence motif, as well as related nucleic acid molecules, expression vectors, recombinant host cells, libraries, and methods and uses.

The invention provides a method for evaluating an aroma enhancement and quality improvement mechanism of flavors and fragrances and application of the method. The method comprises the following steps: (1) taking a sample to be evaluated, and carrying out gas chromatography-mass spectrometry to obtain chemical components; (2) candidate flavor components and corresponding olfactory receptors; (3) screening in the database to obtain a human olfactory receptor; (4) screening out a core olfactory receptor; (5) screening out core flavor components; and (6) carrying out molecular simulation docking on the core olfactory receptor and the core flavor component, screening an optimal binding conformation, and carrying out interaction mechanism verification. According to the method provided by the invention, a set of comprehensive evaluation system taking'multi-component-multi-target 'as a core mechanism is constructed, the problem can be deeply explained and predicted so as to clarify the underlying principle of perfuming and synergy, clear guidance can be provided for a perfumed incense imitating and creating technology, accurate perfumed incense imitating and creating is realized, and the perfumed incense imitating and creating cost is effectively reduced.

Described herein are olfactory receptor proteins having modified C-terminal domains containing specific amino acid sequence motifs, as well as related nucleic acid molecules, expression vectors, recombinant host cells, libraries, and methods and uses.

To provide a determination method or the like capable of determining a health condition of an object by using an olfactory sensor.SOLUTION: In the determination method, a computer executes processing of acquiring a response signal with respect to a target subject derived from a determination target over time detected by using an olfactory sensor including a cell having an olfactory receptor and responding to an odor molecule, and determining a health condition of the determination target based on a profile of the acquired response signal with respect to the target subject over time and a first profile obtained from a response signal with respect to a subject derived from a healthy subject and a second profile obtained from a response signal with respect to a subject derived from a non-healthy subject.SELECTED DRAWING: Figure 1

The present invention relates to a composition for preventing or ameliorating hair loss. The composition for preventing or ameliorating hair loss, according to the present invention, is a composition comprising sandal pentanol, in which, when diffused into the air, the ingredient diffused into the air comes into contact with hair follicles in the human scalp, thereby binding to olfactory receptor 2AT4 (OR2AT4), which is an olfactory cell receptor of hair follicles, and activating IGF-1 protein, which extends the anagen phase of hair follicle cells. Thus, the composition for preventing or ameliorating hair loss can provide the effect of preventing or ameliorating hair loss without being applied onto the scalp.

To provide a cancer inspection technique using an olfactory receptor.SOLUTION: A test agent for a cancer type a, comprising an insect olfactory receptor protein A having reactivity with the cancer type a and an insect olfactory receptor protein C having no reactivity with a cancer including the cancer type a.SELECTED DRAWING: None

Genetically modified cells (e.g., recombinant mammalian cells) and methods for improving the expression / functional response of olfactory receptors are described herein. Genetically modified cells and methods can generally modulate the levels of chaperone proteins that help target receptors to the cell membrane. Genetically modified cells and methods can also provide a platform for use in studying the function of a broader group of chemosensory receptors, including taste receptors and TRP channels.

To provide a technique for improving response activity of an olfactory receptor to a chemical substance.SOLUTION: To introduce a mutation into a specific site in an insect olfactory receptor protein so as to satisfy specific conditions.SELECTED DRAWING: None

The invention discloses a method for predicting interaction of aroma substances based on an XGBoost algorithm. The method comprises the following steps: constructing an aroma substance interaction relation database; the XGBoost model is trained by taking the aroma substance interaction relationship database as a training test data set, the contribution degree of the characteristics to the prediction result is calculated by using the SHAP, important characteristic parameters are selected, the XGBoost model is trained again by taking the important characteristic parameters as model input, and an optimal prediction model is obtained. And obtaining important characteristic parameters of the to-be-tested aroma substance combination, and inputting the important characteristic parameters into the optimal prediction model to complete prediction. According to the method, XGBoost and SHAP are adopted to carry out correlation modeling on the interaction relationship among OI, binding energy and aroma substances, so that deep fusion of aroma activity values, aroma intensity and olfactory receptor binding energy is realized; the prediction precision is remarkably improved through multi-dimensional data integration, and the method has wide applicability and good application prospects.

The invention relates to the identification of or5a2 as an olfactory receptor that binds to natural musk and synthetic musks. The invention encompasses the use of the interaction of or5a2 polypeptides and nitromusk, polycyclic musk, macrocyclic musk, and alicyclic musks as the basis for screening assays for agents that specifically modulate the activity of the or of the invention.

To provide a method for screening an olfactory receptor protein or its combination useful in cancer examination.SOLUTION: A method for screening an olfactory receptor protein or a combination thereof, comprising testing the reactivity of a test olfactory receptor protein to cancer, and selecting at least one selected from the group consisting of (1) a combination of the olfactory receptor protein A and the olfactory receptor protein C, (2) a combination of the olfactory receptor protein A ' and the olfactory receptor protein B, and (3) the olfactory receptor protein B '.SELECTED DRAWING: None

The invention discloses a relieving composition for regulating expression of multivariate receptor protein, a method, application and a cosmetic. The relieving composition comprises the following main components: a buckthorn root extract and a pogostemon cablin extract; the preparation method comprises the following steps: grinding a raw material, namely, a withanolaria nipponica stem block, and extracting through alcohol extraction and biological fermentation processes to obtain the withanolaria nipponica root extract; pogostemon cablin plants are used as raw materials, pogostemon cablin extracts are extracted, and auxiliary materials are used for carrying out inclusion treatment on the pogostemon cablin extracts; and mixing and crushing the two components, sieving, and detecting to be qualified to obtain the white-like powdery soothing composition. The soothing composition has new applications of inhibiting expression of TNF-alpha and TRPV-1, increasing the production level of CB2 and endomorphin and activating olfactory receptor protein OR2AT4, and can be used as a functional component of cosmetics with soothing and repairing effects. The invention has the advantages of good raw material application, high bioavailability, stable active components and low possibility of photothermal discoloration.

Olfactory receptor agonists are presented that exhibit (i) a molecular weight of about 50 to about 300 Daltons and (ii) a relative evaporation rate to butyl acetate of about 3 to about 15 for use as a medicament for the prevention and / or treatment of hyperpigmentation of human skin.

The present invention relates to a computer-implemented method for predicting molecular odors, comprising: providing a first database comprising three-dimensional (3D) structures and / or chemical structures of one or more olfactory receptors (OR); training a machine learning model or AI based on the data contained in the first database; providing a second database comprising (i) a (3D) structure of one or more odorant molecules and (ii) a corresponding textual description of an odorant sensation induced by the one or more odorant molecules in the human subject; training a machine learning (ML) model or artificial intelligence (AI) based on the data contained in the second database; fitting the (3D) structure of the one or more odorant molecules with the (3D) structure of the one or more ORs, thereby determining an affinity score for each fit combination of the one or more odorant molecules with the respective olfactory receptors, where the fitting includes using and training an ML model or AI, and where the affinity score is indicative of a degree of fit of the respective odorant molecules with the ORs; creating a comprehensive third database or third data embedding structure / layer comprising data of the first database, data of the second database, and affinity scores determined for each fit combination of a respective odorant molecule with a respective olfactory receptor; providing a (3D) structure of at least the first target odorant molecules not contained in the second database; and predicting, by machine learning or AI, an odor sensation induced in the human subject by at least the first target odor molecule in view of the data contained in the third database or the third data embedding structure / layer.

This invention provides the application of cyperene in the preparation of olfactory receptor Olfr43 specific agonist drugs, relating to the field of small molecule pharmaceutical technology of traditional Chinese medicine. This invention constructs Hana3A... Olfr43‑FLuc‑RLuc A heterologous OR dual-luciferase screening platform revealed that cyperene can activate the downstream pathway of the olfactory receptor Olfr43, significantly increasing intracellular cAMP levels. Cellular experiments further verified that cyperene stimulation significantly increased the mNRA and protein expression levels of Olfr43 in cells, raising downstream cAMP levels. This, in turn, inhibited the expression of lipid synthesis-related genes by phosphorylating CREB, promoting HES-1, and inhibiting DGAT2 protein expression, ultimately reducing TC and TG in the HepG2 hyperlipidemia model. This invention is the first to propose that cyperene can act as a specific agonist targeting the olfactory receptor Olfr43 to regulate downstream cAMP changes. This invention provides a new option for the development of highly effective agonists specifically targeting Olfr43 / OR1A1.

This invention discloses a method and system for identifying the origin and year of dried tangerine peel based on multi-biomimetic sensor fusion. The method includes: acquiring at least two biosensor response signals of the tangerine peel to be tested, wherein the at least two biosensor response signals include a first response signal from an olfactory receptor protein chip and a second response signal from a neuron chip; inputting the first and second response signals into a pre-trained first classification model and a second classification model respectively to obtain a first and second predicted origin and / or year corresponding to the tangerine peel to be tested; fusing the first and second predicted origin and / or year results, and outputting the origin and / or year identification result of the tangerine peel to be tested based on the fused result. This invention significantly improves the accuracy, reliability, and reproducibility of identifying the origin and year of dried tangerine peel, and achieves rapid, non-destructive on-site detection.