Method For Evaluating The Scent Performance Of Perfumes And Perfume Mixtures
A mixture and fragrance technology, applied in the field of identifying its fragrance properties, can solve the problems of olfactory agnosia, inability to recognize smell perception, etc.
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[0118] A. Materials and methods
[0119] A1. DNA structure and plasmid
[0120] Human ORs (SPGs / CNVs) were amplified from human genomic DNA by PCR using specific primers that amplify the entire open reading frame and contain restriction enzyme sites (EcoRI, NotI) for further subcloning into a pCI expression vector (invitrogen) , ApaI and SalI). The amplified ORs were cloned in the multiple cloning site of the Rho-marked pCI vector ( figure 2 .1). PCR reactions were performed using 100 ng of genomic DNA and specific primers for human olfactory receptors. The resulting plasmids were verified by sequencing. Using Primer 3plus software, primers were designed based on parameters including Tm value (melting temperature) between 55 and 65°C, GC-content between 30% and 70% and length bp (Table 2.1) between 18 and 28 (http : / / www.bioinformatics.nl / cgi-bin / primer3plus / primer3plus.cgi). Choose the same restriction enzyme site as in the pCI-vector. Use Webcutter 2.0 software contr...
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