Method For Evaluating The Scent Performance Of Perfumes And Perfume Mixtures

A mixture and fragrance technology, applied in the field of identifying its fragrance properties, can solve the problems of olfactory agnosia, inability to recognize smell perception, etc.

Active Publication Date: 2015-06-24
SYMRISE GMBH & CO KG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0050] 5) Olfactory agnosia: inability to recognize an odor perception;
However, ...

Method used

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  • Method For Evaluating The Scent Performance Of Perfumes And Perfume Mixtures
  • Method For Evaluating The Scent Performance Of Perfumes And Perfume Mixtures
  • Method For Evaluating The Scent Performance Of Perfumes And Perfume Mixtures

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0118] A. Materials and methods

[0119] A1. DNA structure and plasmid

[0120] Human ORs (SPGs / CNVs) were amplified from human genomic DNA by PCR using specific primers that amplify the entire open reading frame and contain restriction enzyme sites (EcoRI, NotI) for further subcloning into a pCI expression vector (invitrogen) , ApaI and SalI). The amplified ORs were cloned in the multiple cloning site of the Rho-marked pCI vector ( figure 2 .1). PCR reactions were performed using 100 ng of genomic DNA and specific primers for human olfactory receptors. The resulting plasmids were verified by sequencing. Using Primer 3plus software, primers were designed based on parameters including Tm value (melting temperature) between 55 and 65°C, GC-content between 30% and 70% and length bp (Table 2.1) between 18 and 28 (http : / / www.bioinformatics.nl / cgi-bin / primer3plus / primer3plus.cgi). Choose the same restriction enzyme site as in the pCI-vector. Use Webcutter 2.0 software contr...

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Abstract

Suggested is a method for evaluating the scent performance of perfumes or perfume mixtures, wherein at least one odorant is brought into contact with an olfactory receptor selected from the group consisting of OR1B1, OR2L8, OR4X2, OR4C16, OR5L1, OR8B4, OR8D2, OR10A6, OR10C1, OR12D2, OR524, OR4E2, OR4P4, OR4K2, OR4C3, OR5I1, OR10Q1 and measuring the response of the receptor.

Description

field of invention [0001] The present invention belongs to the field of fragrance and smell and relates to a method for evaluating and identifying the fragrance properties of a fragrance or a mixture of fragrances by exposing them to specific olfactory receptors Background technique [0002] The human olfactory system has an excellent ability to recognize and distinguish a large number of different chemical constituents and odor molecules. The olfactory system as a chemosensor contributes to social and sexual behavior as well as quality of life due to the ability of odorants to evoke memories and emotions or influence our moods. [0003] Odor perception begins with chemical interactions between odorant molecules and olfactory receptors expressed in the olfactory sensory neuron (OSN). These nerves are located in the olfactory epithelium (OE), which is covered by a mucosal layer that supplies the surface of the olfactory epithelium with fluid / protein-rich secretions. Most of...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/66G01N33/566G01N2500/04G01N33/5041
Inventor 卡文·阿施提巴哈冈特·基赛曼汉斯·哈特约翰尼斯·潘特
Owner SYMRISE GMBH & CO KG
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