The invention provides a preparation method for heart quieting and nerve soothing capsules. The preparation method comprises the following steps: weighing 100 grams of coptis chinensis, 50 grams of ambers, 250 grams of acorus gramineus, 417 grams of processed radix polygalae, 417 grams of wolfiporia extensa, 417 grams of salvia miltiorrhiza, 250 grams of licorice, 417 grams of red dates, 833 grams of wheat, 833 grams of calcined magnets and 833 grams of mother pearls, adding water in an amount which is 10 times the weight of the medicinal materials, performing impregnation for 0.5 h, performing decoction for 1 h, performing filtration, adding water in an amount which is 8 times of the weight of the medicinal materials into decoction dregs, performing decoction for 1 h, filtering medicinal liquor, mixing the decoction, concentrating the mixed decoction to relative density of 1.05 at 60 DEG C, adding ethanol until the volumetric ethanol content reaches 75 percent, performing stirring, standing and filtration, concentrating the filtrate to relative density of 1.25 at 65 DEG C, recycling the ethanol to obtain a concentrated solution, performing supercritical extraction to obtain supercritical extract, continuing concentration, drying the concentrated solution, and performing granulation and capsule filling to obtain the heart quieting and nerve soothing capsules. Special concentration and drying equipment is adopted, and the prepared heart quieting and nerve soothing capsules are high in extract content, and are found to be applied to preparation of a medicament for suppressing proliferation of HEK-293 cells.