Method for inducing de-differentiation of mature cells

A de-differentiation and cell technology, applied in the biological field, can solve problems such as cell canceration

Active Publication Date: 2013-05-08
BEIJING ZKZKTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the dedifferentiation research mainly adopts the method of transgenic, but due to the change of the genome, the cells face the risk of cancer

Method used

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  • Method for inducing de-differentiation of mature cells
  • Method for inducing de-differentiation of mature cells
  • Method for inducing de-differentiation of mature cells

Examples

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Embodiment 1

[0040] Example 1. Inducing dedifferentiation of mature cells to form cells with stem cell function

[0041] 1. Method for inducing dedifferentiation of mature cells to form cells with stem cell function

[0042] 1. Preparation of low-attachment culture dishes

[0043] Preparation of 2×DMEM basal liquid medium: Dissolve 13.4 g of high-sugar medium powder (Gibco) and 3.7 g of sodium bicarbonate in 500 ml of deionized water, and filter through a 0.22 μ membrane to obtain 2×DMEM basal medium.

[0044] The above high glucose medium powder was purchased from Gibco.

[0045] 2×DMEM basal liquid medium can also be configured as follows: dissolve the following substances with water to the following concentration, and make up the volume to 1L: anhydrous calcium chloride (2H 2 O) 265.00mg / L, L-serine 42.00mg / L, ferric nitrate (9H2O) 0.10mg / L, L-threonine 95.00mg / L, potassium chloride 400.00mg / L, 20L-tryptophan 16.00mg / L, anhydrous magnesium sulfate 97.67mg / L, L-tyrosine 72.00mg / L, so...

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Abstract

The invention discloses a method for inducing de-differentiation of mature cells. The method for inducing de-differentiation of the mature cells comprises the following step of: culturing the mature cells in a solid medium to obtain globular cells, wherein the globular cells have the totipotency of embryonic stem cells, and the solid medium is obtained by mixing a 2*DMEM basal medium and a coagulant. Experiments prove that human embryonic kidney 293 (HEK 293) epithelial cells with a differentiating function grow in a three-dimensional globular shape through a low adhesion surface culture method; and experimental results show that the three-dimensional globular cultured HEK 293 cells have de-differentiation, and the properties of embryonic stem cells and renal progenitor cells are obtained.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for inducing dedifferentiation of mature cells. Background technique [0002] Dedifferentiation is the conversion of a differentiated cell type into another, more primitive cell type with more differentiation potential, such as an embryonic stem cell or precursor cell. The study of dedifferentiation is of great significance to the scientific research of cell biology and the clinical application of stem cells. At present, dedifferentiation research mainly adopts the method of transgenic, but due to the change of genome, the cells face the risk of canceration. Cell fate is not only regulated by internal genes, but also the influence of the external environment is critical. If the cell microenvironment can be regulated by physical means to achieve cell dedifferentiation, the risk of cell canceration caused by transgene dedifferentiation will be avoided. Contents of the inven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0735C12N5/073
Inventor 苏冠男赵燕南魏建树陈冰肖志峰戴建武
Owner BEIJING ZKZKTECH CO LTD
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