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Fructus cnidii extract and preparation and application thereof

A technology of Cnidium Fructus extract and Cnidium chinensis, applied in the field of traditional Chinese medicine, can solve problems such as unknown action mechanism of Cnidium spp.

Pending Publication Date: 2018-10-09
INST OF BASIC THEORY OF TCM CHINA ACADEMY OF CHINESE MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It is important to understand the efficacy of cnidium from the molecular aspect, but the mechanism of action of cnidium is still unknown

Method used

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  • Fructus cnidii extract and preparation and application thereof
  • Fructus cnidii extract and preparation and application thereof
  • Fructus cnidii extract and preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0075] 1 Materials and methods

[0076] 1.1 Drugs and reagents

[0077] The traditional Chinese medicine Cnidium Fructus (purchased in Beijing Tongrentang Dongsi Pharmacy, is the dry and mature fruit of Umbelliferae Cnidium), TRPV1plasmid (Origene, RC217653), plasmid GFP (Beijing Aisi Yipu), DMEM cell culture medium, Fetal Bovine Serum, penicillin / streptomycin (Gibco), Lipofectamine2000 (invitrogen), Capsaicin, DMSO, Sodium chloride, Potassium chloride, Cesium chloride, EGTA, HEPES, Magnesium chloride hexahydrate, D-glucose, Calcium chloride (Sigma).

[0078] 1.2 Instruments

[0079] 300g sealed swing type crushing XL-06B (Guangzhou Xulang), rotary evaporator RE-52 (Shanghai Hongxin), digital constant temperature water bath HH-6 (Changzhou Runhua), SHZIII circulating water vacuum pump (Shanghai Asia Rong), EPC-10Quattro patch clamp amplifier (Heka), PatchMaster data acquisition software (Heka), P-97 pulling instrument (Sutter Instruments), IGOR-Pro analysis software (WaveMet...

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Abstract

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a fructus cnidii extract and preparation and application thereof. The research is capable of using thefructus cnidii extract (aqueous extraction and alcohol extraction) as a research object, an electrophysiology patch clamp technology is combined, and in an HEK 293 cell for instantly transferring a human TRPV1, whether the fructus cnidii extract can activate or inhibit the TRPV1 is tested, so molecular targets of fructus cnidii for developing pharmacodynamic effects are studied. It is firstly discovered that both of an aqueous extract and an alcohol extract of the fructus cnidii activate a TRPV1 channel, and a transmembrane current is generated. So, the pungent-warm property of the fructus cnidii can be a result of TRPV1 activation, the partial efficacy of the fructus cnidii can be achieved through the TRPV1 channel, and the TRPV1 is one of the molecular targets for generating the effectsof warming kidney and tonifying Yang, killing parasites to relieve itching.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to an extract of Fructus Cnidii and its preparation and application. Background technique [0002] Fructus Cnidii was first included in "Shen Nong's Materia Medica", and it has been used in medical practice for thousands of years. It is also known as wild fennel and wild coriander. [0003] "Shen Nong's Materia Medica" lists Cnidium Fructus as the top grade, and summarizes its nature and flavor effects: "bitter, flat. Housewives have swelling and pain in the genitals, men's impotence, damp itching, eliminating numbness, sharpening joints, epilepsy and malignant wounds (sores) .” Shortly thereafter, the "Materia Medica Collection" (Grass and Trees Top Grade) recorded as "bitter, pungent, sweet, flat, and non-toxic. It is mainly used for swelling and pain in the vagina of women, and itching of impotence in men. , epilepsy, malignant sores. Warming the middle an...

Claims

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Application Information

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IPC IPC(8): A61K36/234A61P17/04A61P29/00
CPCA61P17/04A61P29/00A61K36/234
Inventor 赵红霞杨桢汪文来陶旭光刘珍洪马少丹
Owner INST OF BASIC THEORY OF TCM CHINA ACADEMY OF CHINESE MEDICAL SCI
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