The invention discloses an AuNPs-
DNA three-dimensional
nano device, a preparation method thereof and application of the AuNPs-
DNA three-dimensional
nano device in FEN1
enzyme detection.The SR
DNA with the 3'end modified with FAM and the 5 'end modified with-SH serves as a
signal output molecule, meanwhile, long-chain
DNA walker is modified on the surfaces of gold nanoparticles to serve as a walking foot of the DNA
nano device, SR can be hybridized with the
DNA walker, and a
lambda exonuclease (
lambda-EXO) cleavage site is generated; in the presence of
a DNA protection fragment (P), P and DW are hybridized to form double strands, hybridization of DW and SR is prevented, and because an
enzyme recognition site of SR is protected, no
fluorescence signal is emitted. According to the constructed three-dimensional DNA nano device, in the presence of FEN1, due to the fact that FEN1 cuts 5 'Flap,
lambda-EXO
cutting sites are generated, DW is hybridized with SR after being released, more lambda-EXO
cutting sites are generated,
fluorescence signals are released, and sensitive, simple and convenient detection of FEN1 can be achieved through the
fluorescence signals through a
fluorescence biosensor.