The present invention provides a laser-guided electrospinning direct writing device capable of realizing long-time controllable preparation and precise positioning of single uniform micro/nanostructure. The device is provided with a controller, a high-voltage relay, an electrostatic high-voltage [power] source, a hollow nozzle, a collimating focusing lens set, optical fibers, a sealing adjusting part, a laser, a solution feed pump, a solution feed pipe, a collecting board and a motion platform, wherein the controller is connected with the high-voltage relay; ends of the high-voltage relay are respectively connected with the electrostatic high-voltage [power] source, the collecting board and a conductor nozzle arranged at the front end of the hollow nozzle; the sealing adjusting part is in a sealing connection with the rear end of the hollow nozzle; the optical fibers pass through the center of the sealing adjusting part and are fixedly connected with the sealing adjusting part; the collimating focusing lens set is arranged at the front end of the optical fibers; the rear end of the optical fibers comes out of the hollow nozzle and is connected with the laser; the solution feed pump is communicated with the hollow nozzle through the solution feed pipe; and the collecting board is fixed on the motion platform and faces the hollow nozzle. The laser constraints the disordered motion of spinning jet flow and guides the spinning jet flow to deposit at the laser spot of the collecting board.