Construction method of high-throughput ratio type chip sensor for simultaneously detecting various porcine diarrhea coronaviruses

Abstract

The invention provides the construction method of the high-throughput ratio type chip sensor for simultaneously detecting various porcine diarrhea coronaviruses, and the change of the color of the high-throughput ratio type electrode substrate and the Prussian blue can directly read a signal, so that the simultaneous detection of the three porcine diarrhea coronaviruses is realized. The preparation method comprises the following steps: step 1, preparing ZnIn2S4/3DNG; and step 2, constructing a high-throughput ratio type chip immunosensor for simultaneously detecting PEDV (porcine epidemic diarrhea virus), TGEV (transmissible gastroenteritis virus) and PDCoV (PDCoV). According to the constructed high-throughput ratio type chip immunosensor, three detection areas and corresponding signal output areas are prepared, a blank area serving as a reference is added to improve the accuracy, an antibody is introduced to serve as a recognition element to construct an immunosensing platform, and a 3D printed mold is added, so that a detected object can be separated from the signal output areas; each detection area is separated, interference is reduced, three detection objects can be specifically detected at the same time, and the detection efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of photoelectrochemical biosensing, and mainly relates to a method for constructing a photoelectrochemical immunosensor, in particular to a method for constructing a high-throughput ratio-type chip sensor for simultaneous detection of multiple porcine diarrhea-like coronaviruses. Background technique [0002] Photochromic visual sensor is a new type of sensing technology that combines photochromic devices with photoelectrochemical sensors. In response to changes in optical properties, electrochromic materials can produce different color-changing effects under different current signals to achieve the purpose of visual quantitative analysis of detection objects. This technology successfully converts the signal output part of photoelectrochemical sensing technology by The electrochemical workstation is simplified to a color change. The photochromic visual sensor has the advantages of simple operation steps, di...

AI Technical Summary

Problems solved by technology

Among them, serological detection methods such as ELISA and immune chromatography are low in cost, simple and fast in operation, and can meet the detection of large quantities of samples. They are widely used in primary veterinary clinical diagnosis and serum antibody monitoring, but they also have relatively low specificity and sensitivity. Shortcomings

However, traditional molecular biology detection methods such as PCR, multiplex PCR, fluorescent quantitative PCR and other technologies have been greatly improved in specificity and sensitivity, realizing the qualitative to quantitative analysis of pathogens, and the identification and detection of single pathogens to multiple pathogens , which improves the detection efficiency and accuracy, can quickly identify a variety of enteric pathogens and quickly distinguish different genotypes, but still requires high test conditions and costs and is not suitable for large-scale clinical samples and primary veterinary diagnosis

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