Electrochemical fast sweep voltammetry method with high-order repeatability and reproducibility and analysis application of electrochemical fast sweep voltammetry method

Abstract

The invention discloses an electrochemical fast sweep voltammetry method with high-order repeatability and reproducibility and analysis application thereof. A data form presented by the method has the characteristics of obvious high-order repeatability and repeatability, and data results in hundreds of cycles are highly consistent. The final concentration of TdT (0.1-190 U / mL) or PP (0-9 mM) in a mixed reaction solution is changed, an FSCV is adopted to test a sensor, the initial potential is set to be 0 V, the termination potential is set to be 1 V, the scanning speed is set to be 400 V / s, and on the basis, the fast sweep method is applied to the analysis and detection of TdT and a small-molecule inhibitor of TdT. The method has the advantages that the sensitivity is high, the specificity is strong, the analysis time is short, the response speed is high, the preparation is simple, the electrochemical signals with high-order repeatability and reproducibility can be output, and the low-concentration TdT activity analysis can be well realized; the detection limits are (1) 0.067 U / mL, (2) 0.1 U / mL, (3) 0.067 U / mL and (4) 0.1 U / mL respectively, the inhibitor detection IC50 values are 1.7 mM, 1.6 mM, 1.5 mM and 1.7 mM respectively, and the application prospect is good.

Description

technical field

[0001] The present invention relates to two fields: electrochemical analysis technology and biosensing, especially relates to an electrochemical fast-sweep voltammetry method with high-order repeatability, and is applied to terminal deoxynucleotidyl transferase and its small molecule inhibition Agent biosensing analysis. Background technique

[0002] Terminal deoxynucleotidyl transferase (TdT) differs from other DNA polymerases in its special ability to incorporate nucleotides into the 3′-OH terminus of single-stranded DNA in a template-independent manner. Expression of the TdT gene is normally suppressed in most human cells but is abnormally high in acute lymphoblastic leukemia cells and some other leukemia-associated cells. It is worth noting that acute lymphoblastic leukemia accounts for about 25% of the most common cancers in childhood, so the detection of TdT is crucial for the development of cancer-related drugs and basic biochemical research. Despite...

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