The invention relates to a qualitative and quantitative method for liensinine
perchlorate. The qualitative method comprises the following steps: weighing 1mg of liensinine
perchlorate and 1mg of liensinine, uniformly
grinding and tabletting the liensinine
perchlorate and the liensinine with 100mg of
potassium bromide respectively, acquiring an
infrared spectrum of 4000-400cm <-1 > by adopting an
infrared spectrometer, and comparing spectrograms to find that the peak shapes of the liensinine perchlorate and the liensinine are similar, the peak shapes of the liensinine perchlorate and the liensinine perchlorate are common characteristic peaks and the peak shapes of the liensinine perchlorate are unique characteristic peaks of the liensinine perchlorate and the liensinine are common characteristic peaks and the peak shapes of the The quantitative method comprises the following steps: weighing a series of liensinine perchlorate reference substances with different masses, uniformly
grinding and tabletting the liensinine perchlorate reference substances and 100mg of
potassium bromide respectively, collecting an
infrared spectrum, carrying out integration on a 625cm <-1 > characteristic peak to obtain a
peak area, and drawing a
standard curve by taking the
peak area as a
vertical coordinate and taking the sampling
mass of the reference substances as a horizontal coordinate; the method comprises the following steps: weighing a sample to be detected, collecting an infrared spectrum, integrating a 625cm <-1 > characteristic peak to obtain a
peak area, substituting the peak area into the
standard curve equation, calculating the
mass of the liensinine perchlorate in the sample, and substituting the
mass into the content calculation formula to obtain the content of the liensinine perchlorate in the sample. The method is good in precision,
repeatability and stability, wide in
linear range, high in
linear correlation coefficient and high in
recovery rate, the problem that liensinine interference cannot be avoided in an existing
content determination method is effectively solved, whether liensinine perchlorate is generated or not can be qualitatively judged, and the quantity of liensinine perchlorate generated can be quantitatively detected; and a feasible new method is provided for production
process optimization and quality evaluation of liensinine perchlorate.