753 results about "Multiple methods" patented technology

Methods, systems, devices and kits for performing lung volume reduction in patients suffering from chronic obstructive pulmonary disease or other conditions using and comprising minimally invasive instruments introduced through the mouth (endotracheally) to isolate a target lung tissue segment from other regions of the lung and reduce lung volume. Isolation is achieved by deploying an obstructive device in a lung passageway leading to the target lung tissue segment. Once the obstructive device is anchored in place, the segment can be aspirated through the device. This may be achieved by a number of methods, including coupling an aspiration catheter to an inlet port on the obstruction device and aspirating through the port. Or, providing the port with a valve which allows outflow of gas from the isolated lung tissue segment during expiration of the respiratory cycle but prevents inflow of air during inspiration. In addition, a number of other methods may be used. The obstructive device may remain as an implant, to maintain isolation and optionally allow subsequent aspiration, or the device maybe removed at any time.

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

Systems and methods for returning results of services within a distributed computing environment are provided. After a client invokes one or more functions of a service, results of the function(s) may be returned to the client in a plurality of ways: for example, in a message, in a space (e.g., a network-addressable storage location), in a space wherein the client is notified via an event, using an advertisement returned in a message, using an advertisement returned in a space, and using an advertisement returned in a space wherein the client is notified via an event. The advertisement may include the information necessary to access and read the results in a storage location such as a space. A schema for the service may specify a plurality of messages which are usable to invoke the function(s) of the service. The messages, results, and advertisements may be expressed in a platform-independent and / or programming-language-independent data representation language such as XML. The availability of these plurality of methods may enhance the flexibility and adaptability of the distributed computing environment for a variety of situations, such as for clients having differing capabilities. For additional flexibility, results may also be efficiently passed to another service.

In one embodiment, a method for depositing a material on a substrate is provided which includes positioning a substrate containing a contact within a process chamber, exposing the substrate to at least one pretreatment step and depositing a fill the contact vias by an electroless deposition process. The pretreatment step contains multiple processes for exposing the substrate to a wet-clean solution, a hydrogen fluoride solution, a tungstate solution, a palladium activation solution, an acidic rinse solution, a complexing agent solution or combinations thereof. Generally, the HARC via contains a tungsten oxide surface and the shallow contact via may contain a tungsten silicide surface. In some example, the substrate is pretreated such that both vias are filled at substantially the same time by a nickel-containing material through an electroless deposition process.

The E-Grid Sub-Network Load Manager operates to regulate the demands presented by the vehicles to the associated Sub-Network thereby to spread the load presented to the service disconnect over time to enable the controllable charging of a large number of vehicles. The load management can be implemented by a number of methodologies, including: queuing requests and serving each request in sequence until satisfaction; queuing requests and cycling through the requests, partially serving each request, then proceeding to the next until the cyclic partial charging service has satisfied all requests; ordering requests pursuant to a percentage of recharge required measurement; ordering requests on an estimated connection time metric; ordering requests on a predetermined level of service basis; and the like. It is evident that a number of these methods can be concurrently employed thereby to serve all of the vehicles in the most efficient manner that can be determined.

Systems and methods are disclosed for routing callers to agents in a contact center utilizing a multi-layer processing approach to matching a caller to an agent. A first layer of processing may include two or more different computer models or methods for scoring or determining caller-agent pairs in a routing center. The output of the first layer may be received by a second layer of processing for balancing or weighting the outputs and selecting a final caller-agent match. The two or more methods may include conventional queue based routing, performance based routing, pattern matching algorithms, affinity matching, and the like. The output or scores of the two or more methods may be processed be the second layer of processing to select a caller-agent pair and cause the caller to be routed to a particular agent.

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

Systems and methods are disclosed for routing callers to agents in a contact center utilizing a multi-layer processing approach to matching a caller to an agent. A first layer of processing may include two or more different computer models or methods for scoring or determining caller-agent pairs in a routing center. The output of the first layer may be received by a second layer of processing for balancing or weighting the outputs and selecting a final caller-agent match. The two or more methods may include conventional queue based routing, performance based routing, pattern matching algorithms, affinity matching, and the like. The output or scores of the two or more methods may be processed be the second layer of processing to select a caller-agent pair and cause the caller to be routed to a particular agent.

Disclosed is a method for sharply reducing diurnal breathing loss emissions from automotive evaporative emissions control systems by providing multiple layers, or stages, of adsorbents. On the fuel source-side of an emissions control system canister, high working capacity carbons are preferred in a first canister (adsorb) region. In subsequent canister region(s) on the vent-side, the preferred adsorbent should exhibit a flat or flattened adsorption isotherm on a volumetric basis and relatively lower capacity for high concentration vapors as compared with the fuel source-side adsorbent. Multiple approaches are described for attaining the preferred properties for the vent-side canister region. One approach is to use a filler and / or voidages as a volumetric diluent for flattening an adsorption isotherm. Another approach is to employ an adsorbent with the desired adsorption isotherm properties and to process it into an appropriate shape or form without necessarily requiring any special provision for dilution. The improved combination of high working capacity carbons on the fuel source-side and preferred lower working capacity adsorbent on the vent-side provides substantially lower diurnal breathing emissions without a significant loss in working capacity or increase in flow restriction compared with known adsorbents used in canister configurations for automotive emissions control systems.

According to the present invention, a rearview mirror comprises a first substrate having a front surface and a rear surface, a reflective coating disposed on a surface of the first substrate, and an electronic circuit component secured to the rear surface of the first substrate. The mirror element may be an electrochromic mirror element comprising a transparent second substrate positioned in front of the first substrate. The electronic component secured to the rear surface may be a component of a drive circuit for the electrochromic mirror element. The rearview mirror element may further comprise electrically conductive tracings provided on the rear surface of the first substrate electrically coupled to the electrical component. The tracings may be used to electrically couple the drive circuit to the electrodes of the electrochromic mirror element. The tracings may be deposited on the rear surface using numerous methods including inkjet printing techniques.

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

The present invention regards a variety of methods and compositions for obtaining epigenetic information, such as DNA methylation patterns, through the preparation, amplification and analysis of Methylome libraries. In several aspects of the present invention, there are methods based on methylation-dependent enrichment or depletion of genomic DNA isolated from cellular and cell-free sources. In additional embodiments, there are methods and compositions for single-step high throughput preparations of Methylome libraries.

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA poly-merase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

Devices with high-power light-emitting diodes (LEDs) for use in human and / or animal phototherapy applications are disclosed. The phototherapy device includes a number of select LEDs for emitting a desired range or ranges of wavelengths of high intensity light for use in treatment. Additionally, the phototherapy treatment includes one or more methods for providing a treatment appropriate to the condition desired to be treated. The phototherapy device provides a diversity of high power light settings, intensity levels, and selectable time intervals.

The present invention is directed to an improved pulse oximetry sensor device, in which the top and bottom portions of the sensor housing are affixed at a crease point, and are foldable towards each other to create an enclosure therebetween for retaining the optical elements of the device. The sensor housing may then be affixed to a patient by any number of methods, including using adhesive on a flexible strap, or by using a Velcro® strap on a flexible strap. Additionally discussed is a method of manufacturing such a device, and methods to reduce the manufacturing costs of an otherwise disposable device.

When there is an obstruction to the passage of air through the back of the mouth and nose, the soft palate and the bell collide, producing a vibration called snoring. There are various ways of reducing or eliminating snoring, nasal masks introducing air pressure to the throat (CPAP), surgery of the throat (OPPBA) and devices that are introduced into the mouth to cause a mandibular advancement what increases the oropharyngeal area, improving ventilation, among other solutions. The present invention disclosure an intraocclusal removable device in the form of a “U” that is placed covering all of the upper jaw teeth, wherein two steps, one in each extreme of the lower part of the element, which impede the mandible be closed completely on its normal occlusion, forcing it to produce a forward displacement of the lower jaw.

Stimulation of target cells using light, e.g., in vivo or in vitro, is implemented using a variety of methods and devices. One example involves a vector for delivering a light-activated molecule comprising a nucleic acid sequence that codes for light-activated molecule. The light-activated molecule includes a modification to a location near the all-trans retinal Schiff base, e.g., to extends the duration time of the open state. Other aspects and embodiments are directed to systems, methods, kits, compositions of matter and molecules for ion channels or pumps or for controlling currents in a cell (e.g., in in vivo and in vitro environments).

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

A system for distribution of video and audio data, the system including a wireless device operating in a wireless network, a protocol stack, a dispatcher of video and audio data, a storage server, and a video encoder. Multiple methods for using video and audio data, including, among others, methods for optimizing use of mobile radio bandwidth, optimizing use of technical limitations in wireless devices, for allowing users to use premium SMS to interact with the data distribution system, and for verifying the status of a user.

The present invention provides improved methods, systems, devices and kits for performing lung volume reduction in patients suffering from chronic obstructive pulmonary disease or other conditions where isolation of a lung segment or reduction of lung volume is desired. The methods are minimally invasive with instruments being introduced through the mouth (endotracheally) and rely on isolating the target lung tissue segment from other regions of the lung. Isolation is achieved by deploying an obstructive device in a lung passageway leading to the target lung tissue segment. Once the obstructive device is anchored in place, the segment can be aspirated through the device. This may be achieved by a number of methods, including coupling an aspiration catheter to an inlet port on the obstruction device and aspirating through the port. Or, providing the port with a valve which allows outflow of gas from the isolated lung tissue segment during expiration of the respiratory cycle but prevents inflow of air during inspiration. In addition, a number of other methods may be used. The obstructive device may remain as an implant, to maintain isolation and optionally allow subsequent aspiration, or the device may be removed at any time.

A network combining wireless and wired elements is described, using a multi-slot modular mesh node to house diverse transceiver elements (e.g. IR, Wi-Fi, Powerline). A radio agnostic tree based mesh network is formed, based on what type of wireless links are formed on the uplink and downlink of the backhaul and what type of radios are used for the Access Points AP. In addition to servicing IP based clients (e.g. Wi-Fi, WiMax, Bluetooth), the modular mesh nodes APs may also serve as receivers / collectors for low cost chirp devices. The method of transport is standard IP based packets yet security is inherent in this chirp-based implementation: only mesh nodes are privy to the routing tables that indicate that packet addresses are not IP. Multiple methods to obfuscate packet flow are presented. An organic approach to providing category / class based form of data type identification is proposed, to support a (dynamic) M2M Social Network Applications in-device discovery, registration and control are presented.

Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but is also highly specific and capable of discriminating highly homologous nucleic acid target sequences. The method produces DNA:RNA hybrids which can be detected by a variety of methods.

Tissue visualization devices and variations thereof are described herein where such devices may utilize a variety of methods for facilitating clearing of the device of opaque bodily fluids and sealing between the device and the underlying tissue surface. Additionally, methods and devices for enhancing navigation of the device through a patient body are also described.

The invention provides an artificial spinal disc prosthesis that can be implanted to replace a damaged natural spinal disc. The implant includes a synthetic polymer ring. The polymers of the ring are oriented along a common pitch angle relative to a common central axis. Orientation of the polymers provides the ring with added strength and durability. Each synthetic polymer ring further comprises an exterior surface and an interior surface thereby forming a hollow area wherein an additional ring or a nucleus may be enclosed. A pair of angulated prosthetic endplates for use with the implant allow for insertion of the device from a multitude of approaches.

The invention comprises multiple methods for playing a game of poker over a communication network such as the internet which are simple and easy to learn. In a first method, all participants place bets of equal value into a pot with no additional opportunities to raise or place additional bets. In a second method, players are provided with an option to place bets on various rules of winning, wherein bets are paid depending on the outcome of the three-card flop. In a third method, players are presented with timed intervals during which players can place a fixed bet into the pot to be eligible to stay in the hand. Significantly, all players decide whether to place additional bets simultaneously or during the same time interval rather than sequentially as is done in traditional versions of poker.

Devices and methods are disclosed which provide a mobile communications device with multiple methods of wireless communication which can use one method, such as WiFi, to connect to an independent wireless access point while using another method, such as cellular, to verify the security of the wireless access point. The wireless access point provides two SSID's: one private SSID, which is usually encrypted to prevent access, and one public SSID, which is open to any mobile communications device. The mobile communications device connects to the public SSID and downloads a digital certificate. The mobile communications device then uses its cellular connection to verify the authenticity of the digital certificate with its service provider. If verified, the mobile communications device can make use of the wireless access point.