145 results about "Light activated" patented technology

The invention discloses an intelligent defect detection system and a self-repairing method for a flexible base material RFID tag, and belongs to the technical field of Internet of Things electronic device manufacturing. According to the system, a three-dimensional dynamic scanning system is constructed by integrating a high-resolution image acquisition module, a multispectral sensor array and a mechanical arm motion platform, and surface and internal structure characteristics of a flexible substrate are captured in real time. A defect identification algorithm based on deep learning is combined with a multi-scale convolutional neural network and a transfer learning technology, precise classification of 12 types of defects such as microcracks, conductive layer fractures and base material deformation is realized, and the detection precision reaches 99.2%. A dual-mode self-repairing mechanism is put forward, specifically, nano-silver conductive colloid is injected through a microfluid channel for the defects of the conductive layer, and 3D structure reconstruction is achieved through a controllable temperature field; for substrate damage, a photoresponse shape memory polymer patch is adopted, and molecular-level bonding repair is achieved after ultraviolet light activation. According to the scheme, the detection efficiency is improved by more than 5 times, and the radio frequency performance of the tag is recovered to 98.7% of the initial value after self-repairing.

The invention discloses a system for controllably inducing pyroptosis of tumor cells and application of the system. The system comprises a red light activated Cre recombinase system and an expression vector of a GSDMDNT gene sequence of loxP flanking; the Cre recombinase system comprises: (1) an expression vector 1 containing a photosensitive protein PhyA gene sequence and a CreN59 sequence, and a connecting peptide located between the PhyA gene sequence and the CreN59 sequence; (2) an expression vector 2 containing a nucleoplasm shuttle protein FHY1 gene sequence and a CreC60 sequence, and a connecting peptide located between the FHY1 gene sequence and the CreC60 sequence; and (3) phycocyanobilin is contained. The system disclosed by the invention can be used for artificially and controllably regulating and controlling the expression of the cytotoxic gene GSDMDNT, so that directional and controllable pyroptosis induction is realized. The method can be applied to the fields of tumor therapy, gene therapy, cell biology and the like, and particularly has a wide application prospect in application scenarios requiring accurate control of gene expression.

Systems, devices, and methods are provided for all-optical reconfigurable activation devices for realizing various activations functions having normalized output power. The device and systems disclosed herein include an interferometer comprising a first branch formed of a first waveguide and a second branch formed of a second waveguide. A resonator cavity is coupled to the second first waveguide and at least one phase-shift mechanism is coupled to one of the second waveguide and the resonator cavity. The at least one phase-shift mechanism is configured to control biases of the interferometer to achieve a desired activation function at an output of the interferometer, and an optical amplification mechanism is coupled to the output of the interferometer and configured to add optical gain to the desired activation function.

The invention relates to the technical field of fluorescent probes, in particular to a cascade activated fluorescent probe as well as a preparation method and application thereof. The fluorescent probe has a structure as shown in a general formula (I): the diagnosis and treatment integrated fluorescent probe is obtained by combining an indoline group with an oxidative dehydrogenation reaction mechanism and an organic functional group with a distorted configuration, so that a near-infrared two-region fluorescent signal can be quickly activated under the action of ROS (reactive oxygen species), and a tumor focus is positioned; the system can be used as a light-activated guiding radar, can selectively illuminate a tumor area by using high space-time controllability of light, activates and amplifies the phototherapy activity according to needs, realizes effective killing of in-vivo and in-vitro tumor cells, and has important significance for precise tumor phototherapy under dual-mode imaging guidance.

The invention discloses construction of ROS response type self-delivery carrier-free nanoparticles and application of the ROS response type self-delivery carrier-free nanoparticles in breast cancer treatment, firstly, a TLR agonist R848 synthesizes a dimer TKdR through an ROS sensitive bond in one step, then the dimer TKdR and a free photosensitizer Ce6 are co-assembled to form a nano self-assembly body (psTKdR NAs), and an R848 dimer-based photoactivatable supramolecular self-assembly multi-drug delivery system is constructed, pDT can effectively induce ICD to stimulate an autoimmune system, meanwhile, Ce6 can generate ROS under activation of specific light and further induce breakage of TK to release drugs, and R848 can drive cellular immune response and cause polarization of M1 macrophages and is used for tumor immunotherapy. The ROS-responsive intelligent prodrug is designed and introduced into the construction of a nano system, so that the stability of the drug and the sensitivity of a disease site can be improved, and the synergistic curative effect of an intelligent drug delivery system or a traditional drug combination therapy is expected to be realized.

Methods for targeted therapy are disclosed. In certain embodiments, a method includes injecting nanoparticles into a target site in or adjacent to an eye, irradiating the target site with light from a light source, and activating the nanoparticles with the light.

Example eye treatments detennine an area at a surface of a cornea for delivery of a cross-linking agent. The example treatments disrupt tissue at the area at the surface of the conlea up to a depth corresponding to apical layers of superficial squamous cells of the cornea, e.g., no greater than approximately 10 μm to approximately 15 lm. The example treatments apply a cross-linking agent to the area at the surface of the cornea. The cross-linking agent is transmitted through the disrupted area at a greater rate relative to non disrupted areas of the cornea. The example treatments deliver photoactivating light to the cornea. The photoactivating light activates the cross-linking agent to generate cross-linking activity in the cornea.

The application provides a function gradient doped slab laser device, and relates to the technical field of solid-state lasers.The device comprises a pump source, a pump light shaping element and a laser material.The pump light output by the pump source is guided into the laser material through the pump light shaping element, so that the pump light activates the active ions in the laser material to generate laser light.The doping concentration of the laser material satisfies a pre-set doping concentration function, which is used to represent that the materials at different positions in the laser material correspond to different doping concentrations.The technical scheme provided by the application precisely controls the doping concentration of the laser material.Under the condition that the total heat power is unchanged, the temperature distribution of the laser material along the thickness direction is closer to a uniform distribution state, the thermal gradient and thermal effect of the laser material can be reduced, the thermal deformation and thermal stress of the laser material caused by the temperature gradient can be reduced, and the stability and reliability of the function gradient doped slab laser device in operation can be improved.

To treat corneal ectatic disorders, systems and methods can precisely apply photoactivating light to specified areas of a cornea treated with a cross-linking agent. An example system includes a light source that provides a photoactivating light to photoactivate a cross-linking agent applied to an eye. The system includes optical element(s) that transmit the photoactivating light to the eye according to a pattern defined by a plurality of treatment zones. The treatment zones are delivered to different respective areas on the eye. The plurality of treatment zones includes at least a first treatment zone and a second treatment zone. The first treatment zone provides a first dose of the photoactivating light. The second treatment zone provides a second dose of the photoactivating light. The first dose is greater than the second dose. The first treatment zone is disposed within an inner boundary of the second treatment zone.

The invention relates to a method and a system (10) for displaying a viewing direction-dependent view for a predetermined location. The predetermined location, including its surroundings, is captured from one or more viewing directions (15), with or without one or more objects (12) at the location. One or more objects (12) at the predetermined location are then obscured by a three-dimensional display device (20). Based on the images captured in the preparation phase (200), a display model is calculated. This model allows the predetermined location, including its surroundings, to be displayed using the light-activated elements (22) of the display device (20) for each of the one or more predetermined viewing directions (15) or for each viewing direction (15) within the one or more predetermined solid angles. In the absence of one or more objects (12), the location can be displayed from the respective viewing direction (15). In an application phase (206) a momentary viewing direction (15) is determined, a representation of the predetermined location together with its surroundings in the absence of one or more objects (12) is determined from the momentary viewing direction (15) by means of the display model and is displayed by means of the light-active elements (22) of the display device (20) in relation to the momentary viewing direction.

The method suggests coating the transparent solid with an additional laser radiation absorptive layer of material and then laser texture the coated solid to attain a nanostructured transparent solid with increased transmissivity while the additional coating 5 is removed. This method is applicable but not limited to a substrate transparent solid like glasses of silicate composition, such as fused silica, borosilicates, aluminosilicates, alkali aluminosilicates, float glasses, crown glass, amorphous sapphire etc., crystals, for example crystalline sapphire, laser active medium crystals etc. and polymers, for example polycarbonate, plexiglass etc. It is also applicable to substrates coated with a thin film of 0 above mentioned materials.

This medical system comprises: a balloon catheter having an elongate first shaft and a balloon attached to the first shaft, the balloon catheter being for delivering, to the blood vessel wall of a blood vessel, a drug that can be activated by light emitted from a light irradiation body inserted into a lumen extending through the first shaft; an ultrasonic probe having an elongate second shaft and ultrasonic elements attached to the second shaft, the ultrasonic probe being insertable into the lumen and for transmitting ultrasonic waves toward the blood vessel wall from a plurality of positions on the first shaft along the longitudinal direction thereof and receiving reflected waves of the ultrasonic waves using the ultrasonic elements; and a controller that acquires ultrasonic information relating to the reflected waves received by the ultrasonic elements, calculates the vascular stiffness of the blood vessel corresponding to each of the plurality of positions on the basis of the acquired ultrasonic information, and outputs the calculated vascular stiffness in association with each of the plurality of positions.

A laser activated luminescence system is provided. Another aspect pertains to a system employing a plasma assisted vapor deposition reactor which creates diamond layers on a substrate, in combination with a laser system to at least photoactivate and anneal the diamond layers. Yet another aspect of the present system uses a laser to assist with placement of color centers, such as nitrogen vacancy centers, in diamond. The present method uses lasers to manufacture more than two activated nitrogen vacancy center nodes in a diamond substrate, with nanometer spatial resolution and at a predetermined depth.

The present invention discloses a tumor immune microenvironment-regulated light-activated ferroptosis nanoparticle, a preparation method, and an application thereof, relating to the field of biomedicine technology. The preparation method comprises the following steps: dissolving albumin and ferritin in water, adjusting the pH to alkaline, adding a photosensitizer and lipoic acid, mixing, then adding a solvent, performing a mixing reaction, then adding a cross-linking agent, continuing the reaction, and then centrifuging and drying to obtain the tumor immune microenvironment-regulated light-activated ferroptosis nanoparticle. The tumor immune microenvironment-regulated light-activated ferroptosis nanoparticle reverses the tumor immunosuppressive microenvironment by inducing ferroptosis of tumor cells, activating dendritic cells, and regulating the M1 polarization of M2 tumor-associated macrophages, thereby enhancing the effect of tumor immunotherapy.

Provided herein are various examples of a method of coupling oligonucleotides to a polymer. The method may include selectively irradiating first inactive moieties in a one or more first region of a polymer with light, while not irradiating second inactive moieties in a one or more second region of the polymer, to generate first active moieties in the one or more first region of the polymer. The method may also include coupling the first active moieties to first oligonucleotides. The method may further include irradiating the second inactive moieties in the one or more second region of the polymer with light to generate second active moieties in the one or more second region of the polymer. The method may also include coupling the second active moieties to second oligonucleotides.

The invention relates to a preparation method of a heavy-atom-free photosensitizer based on a BODIPY dye, the photosensitizer comprises a thiocarbamate light cage, not only can generate singlet oxygen (1O2) under red light irradiation, but also can release carbonyl sulfide (COS), and generates hydrogen sulfide (H2S) under physiological conditions through carbonic anhydrase catalysis. By reasonably adjusting the molecular structure, the singlet oxygen quantum yield and hydrogen sulfide release efficiency of the photosensitizer are remarkably improved. The method is the first case which is reported at present and can simultaneously activate and release the organic optical functional micromolecules of H2S and 1O2 through a single beam of red light. The strategy effectively solves the problem of dependence on oxygen in type II photodynamic therapy (PDT), and further effectively improves the effect of PDT hypoxic tumors. In in-vitro and in-vivo experiments, hydrogen sulfide gas and photodynamic synergic treatment trigger pyroptosis of cells, promote maturation of dendritic cells and stimulate antitumor immune response. Therefore, the invention provides a synergistic treatment strategy combining gas, photodynamic and immunotherapy, and has a remarkable effect in the aspect of hypoxic tumor treatment. The photosensitizer material disclosed by the invention is simple to prepare and low in cost, has a wide application prospect, and particularly provides a solution with huge potential for cancer treatment in the field of biomedicine.

An Energy Reducing Maintenance Switch (ERMS) connected to an electrical circuit includes: a switch that activates the ERMS upon being closed or turned-on; a normally-open first contact that closes to activate an instantaneous trip setting of a relay upon activation of the ERMS; and a second contact. The instantaneous trip controls an arc flash in the electrical circuit, and the activated instantaneous trip setting of the relay reduces time of the arc flash in the electrical circuit. Upon activation of the ERMS, the second contact closes to initiate ERMS activation alert in two or more forms.

This invention discloses a photoactivated nanoparticle-based drug delivery material for immunoadjuvants, its preparation method, and its applications. The drug delivery material is constructed from photosensitizer monomers, crosslinking monomers, and immunoadjuvant prodrug monomers within a confined space of a nanoemulsion via a copper-free click polymerization reaction to form crosslinked polymer nanoparticles. The nanoparticle size is controllable, and the composition is precisely defined. Under physiological conditions, the drug delivery material exhibits good colloidal stability and high drug loading. Upon near-infrared light irradiation, the generated ROS can cleave the thioketone bonds, causing the nanoparticles to depolymerize and achieving photo-triggered, controllable release of the immunoadjuvant. Under photodynamic action, this drug delivery material efficiently generates reactive oxygen species to induce immunogenic cell death in tumor cells. Simultaneously, the released immunoadjuvant activates the TLR7 / 8 pathway, promotes dendritic cell maturation, and enhances the systemic anti-tumor immune response, thereby inhibiting the growth of tumors in situ and at distant sites, and reducing the risk of metastasis and recurrence.

The invention belongs to the technical field of phosphorescent materials and information encryption materials, and discloses a light activation time dependent afterglow carbon dot polymer film and a preparation method and application thereof.The preparation method comprises the following steps that o-phenylenediamine serves as a carbon source precursor, isopropanol serves as a solvent, a reaction is conducted for 6-8 h at the temperature of 180-250 DEG C under the closed high-pressure condition, and a CDs solution is obtained; and adding the CDs solution into the ABS resin, uniformly mixing to prepare a polymer film, and curing to obtain the ABS / CDs composite film. The obtained polymer film shows double light activated delayed luminescence, emission is generated at 521 nm and 652 nm respectively, the reversible afterglow discoloration process from red to orange to yellow to green is achieved, and the optical memory effect as long as 40 minutes is shown under the environmental condition. The method is a first carbon dot-based system which realizes a photo-activated TEAC behavior and has a reversible optical memory characteristic. Compared with inorganic and organic materials, the carbon dot polymer film has the advantages of simplicity, convenience, rapidness, no metal, long service life, economy, effectiveness, low toxicity and the like.

The application discloses a kind of photoactivation manganese ion bridging type deoxyribozyme probe preparation and its application in Cistanche inhibiting acetylcholinesterase activity.The probe is pSD@MnO2 Composite structure, which is composed of manganese dioxide nanosheet and functional nucleic acid assembly adsorbed on its surface, the assembly includes S chain labeled with fluorescence / quenching group and containing deoxyribozyme cleavage site, and pD chain partially hybridized with S chain and containing photocleavage site.Its preparation includes nucleic acid probe assembly, MnO2 Nanosheet complexing and purification steps.When applied, AChE catalytic product decomposes MnO2, releases Mn 2+ And nucleic acid chain; after centrifugation, pD chain is cut by ultraviolet light activation trigger, and released DNAzyme is cut under the assistance of Mn 2+ It produces amplified fluorescence signal; by comparing signal change before and after adding inhibitor, inhibition activity can be quantitatively evaluated.The application combines photocontrol activation with enzyme cascade amplification, realizes high sensitivity and operation synchronism of detection process, and is especially suitable for high-throughput rapid screening of AChE inhibitor in Cistanche and other natural products.

The application discloses a kind of photoactivation organic long afterglow material and its preparation method and application.The application of compound of formula (III), formula (IV), formula (V) in photoactivation organic long afterglow material;Structural formula of compound of formula (III), formula (IV), formula (V) is as shown below:formula (III)-(V) as the guest component of photoactivation long afterglow material, the efficiency of generating triplet exciton is higher and its triplet exciton energy transfer to oxygen molecule is stronger, the photoactivation process of long afterglow material prepared by being doped in epoxy polymer is short, and the material after activation can be restored to the state without long afterglow emission by heating annealing, so as to effectively overcome the shortcomings that the current photoactivation long afterglow material needs long activation time and is difficult to restore to unactivated state.

This invention discloses a two-dimensional hydrogen germanene nanosheet, its preparation method, and its applications. The two-dimensional hydrogen germanene nanosheet has an ultrathin layered structure, with covalently modified hydrogen atoms on its surface, a lateral dimension of 100–300 nm, and a thickness of 5–10 nm. When activated by a specific wavelength laser, the two-dimensional hydrogen germanene nanosheet can photoexcite and generate singlet oxygen to destroy bacterial biofilms and kill bacteria. Simultaneously, it consumes local oxygen to create a staged hypoxic microenvironment, thereby inhibiting excessive inflammatory responses. After the light exposure ends, as the local oxygen level naturally recovers, it can further enhance the infection clearance function of immune cells, achieving continuous clearance of residual infection. This invention, through staged dynamic regulation of oxygen partial pressure, synergistically integrates direct bactericidal and immune clearance processes, ensuring antibacterial strength while reducing the risk of tissue damage and infection recurrence. It is suitable for the safe and effective treatment of implant-related biofilm infections.

A sensor has a radiation emitter, a plurality of electrodes, a sensing material (transducer), and a recognition element (receptor, bioreceptor). The radiation element irradiates the sensing material, the plurality of electrodes are in electrical contact with the sensing material, and the recognition element is in contact, either directly or through an interface, with the sensing material. The sensing material receives radiation from the radiation source and interacts with the target material largely through the receptor. Further, the sensing material generates a measurable electrical signal upon interaction with a target molecule, when a potential is applied to the electrodes.

The utility model provides a diaphragm, a diaphragm decoration strip, an atmosphere lamp assembly and a vehicle. The diaphragm comprises a weather-proof layer, a color texture layer, a fluorescent development coating and a base material layer which are sequentially arranged in a stacked mode through the INS technology. The color texture layer is a semitransparent structure layer; the fluorescent developing coating comprises a plurality of developing layers, and fluorescent materials of the plurality of developing layers are different. According to the utility model, the fluorescent developing coating integrated by the plurality of developing layers is arranged in the diaphragm, specific backlight patterns are integrated into the same diaphragm, different fluorescent materials are selected for the patterns of different developing layers, and the patterns formed by the different fluorescent materials can be activated by light with different wave band spectrums so as to be developed; therefore, different appearance effects are presented.

The invention discloses an antibody targeting light activated fluorescent protein Dronpa and related application thereof.The amino acid sequences of CDR-H1, CDR-H2 and CDR-H3 in a heavy chain variable region of the antibody are shown as SEQ ID NO.4-6 respectively, and the amino acid sequences of CDR-L1, CDR-L2 and CDR-L3 in a light chain variable region of the antibody are shown as SEQ ID NO.7-9 respectively. According to the antibody and the application thereof, accurate tool support is provided for detection and analysis of Dronpa protein, and the antibody can be applied to detection of Dronpa protein. Meanwhile, a key technical guarantee is provided for life science experiments (such as living cell imaging and molecular mechanism research) and potential clinical application based on the Dronpa protein.

Example eye treatments detennine an area at a surface of a cornea for delivery of a cross-linking agent. The example treatments disrupt tissue at the area at the surface of the con1ea up to a depth corresponding to apical layers of superficial squamous cells of the cornea, e.g., no greater than approximately 10 μm to approximately 15 lm. The example treatments apply a cross-linking agent to the area at the surface of the cornea. The cross-linking agent is transmitted through the disrupted area at a greater rate relative to non disrupted areas of the cornea. The example treatments deliver photoactivating light to the cornea. The photoactivating light activates the cross-linking agent to generate cross-linking activity in the cornea.

The present disclosure provides an enhanced windshield display for a vehicle. An autonomous vehicle includes a window having a first polymeric material layer and a second polymeric material layer between an inner glass layer and an outer glass layer. The first polymer material layer includes perovskite crystals that are activated by UV light and emit visible light. The second polymer material layer is disposed between the outer glass layer and the first polymer material layer. The second polymer material layer blocks UV sunlight to prevent activation of the perovskite crystals. The vehicle also includes a projector configured to project UV light onto the inner side of the window to form a luminescent image on the window.

The application provides a novel light-activated channel protein VR3.0 and uses thereof. The light-activated channel protein VR3.0 series provided in this application has a wide range of photosensitive wavelengths, higher sensitivity to light response, faster photoresponse kinetics, maintaining stable photocurrent signals under high-frequency stimulation while exhibiting good response amplitude and frequency under light stimulation of various wavelengths (especially white light, natural light). The light-activated channel protein VR3.0 series provided in this application has a clear therapeutic effect on retinal photoreceptor cell degenerative diseases, and can be used to prepare drugs for restoring the photoreceptor function of the retina, restoring the visual or photosensitive ability of subjects, and treating retinal degenerative diseases.

The invention discloses a photo-activated promoter 4 * pLRE-cPAOX1 in pichia pastoris. The photo-activated promoter 4 * pLRE-cPAOX1 is prepared by inserting four photo-response elements pLRE of a neurospora crassa circadian rhythm system into the 5'end of a core promoter of pichia pastoris AOX1 in series; and meanwhile, a PGAP-WC1-Mit1AD expression cassette and a P0472-WC2 expression cassette are simultaneously inserted into the carrier pGAPZ alpha, so that a high-expression chassis strain is constructed. The light-activated promoter can work only in the chassis strain. Wherein the sequence of the WC1-Mit1AD is as shown in SEQ ID NO: 31 and SEQ ID NO: 30, the sequence of the WC2 is as shown in SEQ ID NO: 32, and the sequence of the 4 * pLRE-cPAOX1 is as shown in SEQ ID NO: 29. By adopting the light regulation and control element disclosed by the invention, the target gene expression level of pichia pastoris can be regulated and controlled by controlling the existence and intensity of illumination.

The utility model discloses a light active calcium production stirring device, and particularly relates to a stirring device which comprises a stirring wheel which is divided into an upper conical cover and a lower conical cover according to the structure, a concave groove is formed between the upper conical cover and the lower conical cover, and a plurality of pulp plates arranged in a circumferential array are arranged in the concave groove; the upper conical impeller is rotationally arranged in the upper conical cover, and a preset distance is kept between the lower edge of the upper conical impeller and the section of a port of the upper conical cover; the lower conical impeller is rotationally arranged in the lower conical cover, and a preset distance is kept between the lower edge of the lower conical impeller and the section of the port of the upper conical cover. The stirring device is simple in structure, and a composite stirring system is formed by the stirring wheel, the upper conical impeller and the lower conical impeller, so that the stirring effect is optimized.