This invention discloses a method for preparing
phycocyanin and its related applications, relating to the field of active substance extraction technology. Through a specific sequence of membrane
filtration,
cell disruption, and
chromatography, this invention efficiently and selectively separates and purifies high-purity
phycocyanin from the complex composition of *E. coli*
fermentation broth. Compared to the cumbersome traditional process relying on algal extraction followed by chemical
lysis, this method avoids the
seasonality and geographical limitations of raw materials, achieving high
recovery rates and
highly selective enrichment of
phycocyanin. The process flow is clear, the conditions are mild, and it is easy to scale up, demonstrating good industrial
scalability potential. This invention not only provides a stable, economical, and efficient technical
route for the large-scale preparation of phycocyanin but also lays a
solid foundation for its in-depth application in high-value-added fields such as food, pharmaceuticals, and biological detection.