51 results about "Phycocyanobilin" patented technology

The invention relates to phycocyanin beta subunits fluorescent protein combined with phycoerythrobilin PEB, fusion protein formed by phycocyanin beta subunits fluorescent protein and streptavidin and a mutant thereof, the sequences include sequence 1, sequence 2, sequence 3 and sequence 4; furthermore, the invention discloses a method of the fluorescent protein fused with streptavidin for fluorescent immunological detection directly; phycocyanin beta subunit conserved cysteine residue can be not only combined with phycocyanobilin PCB by a thioether bond, but the fact that the phycocyanin beta subunit conserved cysteine residue can be combined with the phycoerythrobilin PEB by the thioether bond through the genetic engineering can be realized, so as to obtain the novel fluorescent phycocyanin, the spectroscopy of the protein is completely different from that of the phycocyanin beta subunits fluorescent protein combined with PCB, and the protein has high fluorescence efficiency; in addition, as the protein carries His-tag label, purification is not only convenient, but also the dissolubility of the protein can be improved; the protein is combined with the streptavidin to form the fusion protein for fluorescent immunological detection directly, thereby being beneficial to the application of the protein in all kinds of the field.

The invention discloses environment-friendly efficient multifunctional leaf fertilizer and a preparing method thereof and belongs to the technical field of fertilizer. The environment-friendly efficient multifunctional leaf fertilizer is composed of phycoerythrobilin, phycocyanobilin, seaweed elements, tea saponin and fulvic acid potassium. The preparing method is scientific and simple, and the product is environmentally friendly and has various functions of enhancing the stress resistance, promoting growth and preventing and treating insect diseases for crops.

The invention relates to phycocyanin alpha subunits fluorescent protein combined with phycocyanobilin PCB and fusion protein formed by phycocyanin alpha subunits fluorescent protein and streptavidin, the sequences include sequence 1 and sequence 2; furthermore, the invention discloses a method of the fluorescent protein fused with streptavidin for fluorescent immunological detection directly; phycocyanin alpha subunit conserved cysteine residue can be combined with phycocyanobilin PCB by a thioether bond, and the alpha subunit of the fluorescent phycocyanin can be obtained by utilizing escherichia coli through genetic engineering, the spectroscopy of the protein is completely different from that of natural phycocyanin; in addition, as the protein carries His-tag label, purification is not only convenient, but also the dissolubility of the protein can be improved; the protein is combined with the streptavidin to form the fusion protein for fluorescent immunological detection directly, thereby being beneficial to the application of the protein in all kinds of the field.

The invention relates to a near-infrared fluorescent probe with maximum Stoke displacement, belonging to the technical field of specific molecular recognition diagnostic reagents. The invention provides a fluorescent dye with maximum Stoke displacement, which is prepared by coupling phycocyanobilin in phycobiliprotein with a near-infrared fluorescent dye. The fluorescent dye has maximum Stoke displacement reaching above 300nm, has the characteristic of smaller molecular weight, can be used for marking matters with small molecular weight, such as folic acid and the like, and is used for specific fluorescence detection of a tumor marker, an antibody and the like.

The invention discloses nutrition strengthened wheat flour and belongs to edible flour. The nutrition strengthened wheat flour is prepared by the following raw materials in ratio by weight: 50-65 percent of wheat flour, 13-22 percent of isomaltose hypgather, 20-25 percent of colored fruit and vegetable powder and 2-3 percent of stachyose. According to the nutrition strengthened wheat flour, natural vegetables and fruits are pulped and concentrated and are made into nutrition vegetable and fruit powder by spray drying, and then mixed with the isomaltose hypgather and natural nutrient in a certain ratio, and therefore the produced nutrition strengthened wheat flour is rich in various phytochemicals like calcium, iron, zinc, selenium, vitamin B, natural carotenoids, chlorophyll, lycopene, anthocyanin and phycocyanobilin. The nutrition strengthened wheat flour can be made into various colored flour capable of preventing and treating various metabolic diseases like obesity, hypertension, hyperlipidemia, diabetes and cardiovascular and capable of resisting aging.

An optical sensitization material disclosed by the invention is phycocyanobilin prepared by means of genetic engineering. In the sensitization material, a semiconductor electrode is processed by a mode of sensitization of a TiO2 particle electrode, vacuum pumping and drying are carried out in a room temperature, the absorption of the electrode to the sensitization material is completed through electrostatic force absorption and hydrogen bonding interaction, the sensitization material is assembled to a dye-sensitized solar cell, the semiconductor electrode is sensitized so that the conversion of solar energy to electric energy can be directly completed under a light condition, and the function of the phycocyanobilin serving as a novel optical sensitization material is achieved.

The invention relates to a molecular design phycoerythrocyanin beta subunit fluorescent protein combining phycoerythrobilin, fusion protein formed by the phycoerythrocyanin beta subunit fluorescent protein and streptavidin, and mutant thereof, having the sequences 1, 2, 3 and 4. The invention also discloses a method for directly using the fluorescent protein fused with the streptavidin for fluorescence immunoassay; and due to thioether bond, 153th cysteine residue conserved by phycoerythrocyanin beta subunit not only can be combined with phycocyanobilin (PCB), but also can be combined with the phycoerythrobilin (PEB) through genetic engineering, so that a novel fluorescent phycoerythrocyanin can be obtained. The spectrum property of the phycoerythrocyanin is completely different from that of the phycoerythrocyanin beta subunit fluorescent protein combined with the PCB, has higher fluorescence efficiency, and is convenient for purification due to being provided with His-tag label. Furthermore, the dissolubility can be improved, the fusion protein can be formed by the phycoerythrocyanin beta subunit fluorescent protein and the streptavidin, the fluorescent protein can be directly used for fluorescence immunoassay, and the invention is beneficial to the application in various fields.

Methods and materials for producing meso-biliverdin are provided where the methods include reacting phycocyanobilin with an amphoteric compound in a solvent to yield meso-biliverdin.

The invention relates to a molecular design method of gene-coded red fluorescent protein, comprising the following steps of: obtaining a corresponding fusion gene hol:pcyA by fusing a heme oxidase gene hol and a heme reducase gene pcyA; coding and synthesizing phycocyanobilin PCB by the fusion gene; generating gene-coded reversible photochromic or non-reversible photochromic red fluorescent protein after fusing the fusion gene hol:pcyA and a genetic fragment of an apoenzyme proteinochrome structure domain which combines the phycocyanobilin PCB again. The molecular designing method of gene-coded red fluorescent protein can be widely used in the biological fields of fluorescent probes, trace labeling or positioning exogenetic organisms, phototherapy, and the like.

The invention discloses a preparation method of a nostoc sphaeroides grain beverage. The method comprises the following concrete steps of nostoc sphaeroides harvesting and selection, nostoc sphaeroides ion replacement, nostoc sphaeroides enzyme activity passivation, nostoc sphaeroides sterilization treatment, beverage proportioning, filling, pasteurization and packaging. The method has the following beneficial effects that through the ion replacement, the combination with ingredients such as phycocyanobilin, allophycocyanin, phycoerythrobilin, chlorophyll and the like inside nostoc sphaeroidesis performed to form a stable chelate, so that a stable pigment can be formed. Meanwhile, by regulating a PH value and blanching and passivating the enzyme activity, the characteristics of nutritional ingredients of the nostoc sphaeroides is ensured, so that the whole appearance of the whole product is glittering and translucent; the sphere is mellow and full; the mouthfeel is tender and elastic.The functions of thirst quenching, pleasant flavor, rich juice nutrient and the like of the beverage are realized; the effective function, the Q elastic mouthfeel and the glittering and translucent appearance of natural freshwater alga of the nostoc sphaeroides are also realized; in addition, the pleasant flavor in the beverage can be blended into the nostoc sphaeroides.

The invention discloses a pharmaceutical composition for treating diabetic retinopathy and a preparation method thereof. The pharmaceutical composition for treating diabetic retinopathy consists of the following components in parts by weight: coarse grain mixed powder, pseudolaric acid, p-chlorophenoxy isobutyric acid, syringin, phycocyanobilin, friedelin, stigmasterol, formononetin, L-alpha-aminoadipic acid, pancreatin, L-malic acid, vitamin and L-glutamic acid. The pharmaceutical composition disclosed by the invention realizes the effects of controlling blood glucose and treating retinopathy by improving glycometabolism, focusing on blood glucose reduction assisted by the medicinal components for nourishing yin and clearing heat and resolving stasis and by improving the human immunity to enhance the tolerance and resistance against lesion; and moreover, the pharmaceutical composition is simple in technology, realizes a good curative effect and can be promoted for application.

A supercritical cracking process of phycocyanin is provided. The supercritical cracking process comprises the following steps: S1, loading dried spirulina powder into a supercritical extraction kettle, continuously introducing carbon dioxide into the extraction kettle at a constant flow rate, keeping proper pressure and temperature in the extraction kettle, and collecting an extract that is carotenoid, adding an ethanol solution, and collecting an extract that is chlorophyll; S2, adding a cracking agent into residual spirulina powder residues in the extraction kettle, and performing high-temperature cracking to obtain a phycocyanin solution; and S3, carrying out supercritical chromatographic separation on the phycocyanin solution, pumping the phycocyanin solution at a certain sample injection flow rate, pumping the phycocyanin solution at a certain sample injection flow rate in a manner that the supercritical carbon dioxide fluid carries the solvent, and eluting residual solvent impurities in the phycocyanin to obtain the phycocyanin. According to the method, the phycocyanin is accurately extracted and separated, the effects of complete elution and zero solvent residue can be achieved, the quality is controllable, and the safe edible performance of the phycocyanobilin product is guaranteed.

The invention discloses a pharmaceutical composition for treating II-type diabetes and a preparation method of pharmaceutical composition. The pharmaceutical composition comprises the following ingredients in parts by weight: 80-150 parts of a crude fiber mixture, 60-90 parts of phycocyanobilin, 50-80 parts of patuletin, 38-76 parts of diosgenin, 35-70 parts of rutin, 16-30 parts of polyethylene glycol, 15-24 parts of pyridoxine, 12-18 parts of flavonoid glycosides, 10-16 parts of linoleic acid, 10-15 parts of sciadopitysin, 8-13 parts of kaempferol, 8-12 parts of quercetin, 7-11 parts of glutamic acid, 6-10 parts of lysine, 5-10 parts of tannin, 5-8 parts of flunarizine, 4-7 parts of thiamine and 2-6 parts of mixed enzyme. The pharmaceutical composition disclosed by the invention is a combination of traditional Chinese medicine and western medicines, effectively reduces the blood sugar and improves the glucose tolerance level of a muscle body on the basis of regulating the normal diet, regulates various abnormal indicators of the muscle body, enhances the tolerance of long-term drug use of a diabetic patient and reduces drug side effects. The pharmaceutical composition disclosed by the invention is simple in preparation process and convenient to use and has good clinical popularization significance.

The present invention discloses a wild type ApcE2 protein mutant. The mutant is characterized by being obtained by mutation of at least one amino acid in an amino acid sequence shown in a SEQ ID No.1,and wherein the amino acid mutation is selected from at least one of mutation of a amino acid site outside a loop, mutation of an amino acid site inside a loop, and a loop structure sequence with partial deletion mutation. The ApcE2 protein mutant of the present invention has a higher solubility than that before the mutation, and the unit yield expressed in Escherichia coli is remarkably improved. The ApcE2 protein mutant of the invention can be used for fluorescence labeling tissue localization, preparation and extraction of phytochrome P[phi]B, preparation of other phycobiliproteins, such as phycoerythrin PEB and phycocyanobilin PCB, and in vitro recombination of fluorescent phycobiliprotein. The preparation method of the present invention realizes higher unit yield and better solubility of the pigment protein.

The present invention describes peptides comprising phycocyanobilin (PCB), as well as the medical use of said peptides and that of PCB, due to the neuroprotector and/or neuroregenerative effects identified for them. Furthermore, pharmaceutical combinations of said peptides and of PCB with proteins or other peptides with synergic effect justify their use for ischemic or neurodegenerative CNS disease treatment.

The present invention provides a method for inhibiting the UDP-GDH enzyme, which has in itself important beneficial implications, thus strongly enhancing the absorption and circulation of the natural drug curcumin, which in itself is poorly absorbed by the human organism. The method consists in blending curcumin with whole cyanobacterial algae, particularly Aphanizomenon flos aquae (but also Spirulina) or algal extracts concentrating or purifying the cyanobacterial molecules phycocyanin and phycocyanobilin. The method solves the significant problem of poor curcumin absorption through substances that also add their own nutritional and antioxidant activity to the mix.

The invention relates to a molecular design phycoerythrocyanin beta subunit fluorescent protein combining phycocyanobilin (PCB), fusion protein formed by the phycoerythrocyanin beta subunit fluorescent protein and streptavidin, and mutant thereof, having the sequences 1, 2, 3 and 4. The invention also discloses a method for directly using the fluorescent protein fused with the streptavidin for fluorescence immunoassay; and due to thioether bond, 153th cysteine residue conserved by phycoerythrocyanin beta subunit can be combined with the phycocyanobilin (PCB), and is prepared into beta subunit of fluorescent phycoerythrocyanin by escherichia coli of genetic engineering. The spectrum property of the phycoerythrocyanin is completely different from that of the natural phycoerythrocyanin, and is convenient for purification due to being provided with His-tag label. Furthermore, the dissolubility can be improved, the fusion protein can be formed by the phycoerythrocyanin beta subunit fluorescent protein and the streptavidin, the fluorescent protein can be directly used for fluorescence immunoassay, and the invention is beneficial to the application in various fields.

The invention relates to phycocyanin and phycoerythrin beta subunits fluorescent protein combined with phycocyanobilin PCB, fusion protein formed by phycocyanin and phycoerythrin beta subunits fluorescent protein and streptavidin and a mutant thereof, the sequences include sequence 1, sequence 2, sequence 3 and sequence 4; furthermore, the invention discloses a method of the fluorescent protein fused with streptavidin for fluorescent immunological detection directly; phycocyanin and phycoerythrin beta subunit conserved cysteine residue can be combined with phycocyanobilin PCB by a thioether bond, and the beta subunit of the fluorescent phycocyanin and phycoerythrin can be obtained by utilizing escherichia coli through genetic engineering, the spectroscopy of the protein is completely different from that of natural phycocyanin and phycoerythrin; in addition, as the protein carries His-tag label, purification is not only convenient, but also the dissolubility of the protein can be improved; the protein is combined with the streptavidin to form the fusion protein for fluorescent immunological detection directly, thereby being beneficial to the application of the protein in all kinds of the field.

The invention discloses a tablet for treating hysteromyoma and a preparation method thereof. The tablet mainly comprises the following components: aloe-emodin, ajmalicine, fumarine, kiwifruit extract, tamoxifen, maytanprine, N-acetylglucosamine, solasonine, phycocyanobilin, calcium pantothenate, beta-sitosterol, hericium erinaceus polysaccharide, coumarin, nobiletin, coriolus versicolor polysaccharide, and compound vitamin. The tablet also comprises the following excipients: a filling agent, a disintegrating agent, an adhesive, and a lubricating agent. The tablet disclosed by the invention is prepared by adding purely natural extracts into active ingredients of multiple Western medicines in reasonable proportions, and has the effects of promoting blood circulation to arrest pain, reducing swelling and resolving mass, inhibiting the growth of tumor, and resisting canceration of tumor tissues; and as hormone stimulation can quickly improve the blood circulation of hyperplastic endometrium, hyperplastic connective tissues are gradually softened, diminished and vanished. The tablet disclosed by the invention is simple in preparation process, is clinically verified to be safe and effective, is convenient to use, and has very good clinical promotion significance.

The present invention discloses a preparation method of reversible photochromic biliprotein, and is characterized by that it utilizes gene engineering technique to express the apoprotein of photochrome reversible photochromic biliprotein and phycocyanin lyase lyzed from phycocyanin by correspondently catalyzing polycocyanobilin, then uses the lyase or photochrome aproprotein to catalyze phycocyanobilin to make it transfer from phycocyanin and its subunit, and at the same time externally recombined with biliprotein and apoprotein so as to prepare the reversible photochromic biliprotein. Said invention does not use organic solvent and detergent, only uses protein and aqueous solution. It is suitable for food, health-care and medicine function fields.