11 results about "Phycocyanobilin" patented technology

The invention relates to the technical field of remote sensing monitoring, and discloses a lake cyanobacterial bloom detection method and system fused with a remote sensing image. The method comprises the following steps: acquiring a multispectral remote sensing image and a synthetic aperture radar image of a target lake; calculating a phycocyanobilin characteristic index and a chlorophyll fluorescence peak index according to the characteristic wave band reflectivity, and screening pixels meeting discrimination conditions to obtain an optical water bloom distribution mask; calculating a radar inversion phycocyanobilin index through a nonlinear regression model according to the dual-polarization backscattering coefficient to obtain a radar water bloom distribution mask; and fusing the double masks to obtain a cyanobacterial bloom monitoring result. The method solves the problems that an existing cyanobacterial bloom detection method cannot realize all-weather monitoring, lacks cyanobacterial specific recognition capability and is insufficient in reliability of a single data source, and improves the timeliness, accuracy and reliability of cyanobacterial bloom detection.

Disclosed herein is a method for enhancing immune response of a vaccine. The method includes preparing a Phycocyanin compound. The Phycocyanin compound includes at least one of C-phycocyanin (C-PC), Phycoerythrin (PE), Allophycocyanin (APC), phycocyanobilin (PCB), and combinations thereof. The method further includes administering the prepared Phycocyanin along with a vaccine composition to a living body.

The invention relates to a cyanobacterial bloom extraction method based on spectrum and phenological characteristics, and relates to the field of ecology, and the method comprises the steps: selecting a vegetation index PCI which is excellent in cyanobacterial bloom recognition, building a localized phycocyanobilin inversion model, and carrying out the homogeneity test of pixels around a sampling point; by calculating a PCI gradient image time sequence, cyanobacterial bloom and background water boundaries are identified, and a threshold value for distinguishing cyanobacteria and non-cyanobacteria pixels is determined by using a maximum gradient average value; constructing a cyanobacterial bloom recognition model based on the LSTM neural network; calculating cyanobacterial bloom outbreak intensity and coverage area, identifying a hot spot area and a cold spot area, and depicting distribution characteristics of cyanobacterial bloom in space; a PCI time sequence data set is synthesized, the evolution trend of a cyanobacterial bloom key phenological index time sequence is described, the innovation of the method lies in that spectrum and phenological characteristics are fused, cyanobacterial bloom spatial and temporal distribution is accurately analyzed, and guarantee is provided for water environment treatment and drinking water safety.

The application relates to the technical field of remote sensing monitoring, and discloses a lake cyanobacterial bloom detection method and system fusing remote sensing images. The method comprises the following steps: acquiring multispectral remote sensing images and synthetic aperture radar images of a target lake; calculating phycocyanobilin characteristic indexes and chlorophyll fluorescence peak indexes according to characteristic band reflectivity, screening pixels meeting a discrimination condition to obtain an optical water bloom distribution mask; calculating a radar-retrieved phycocyanobilin index according to a dual-polarization backscattering coefficient through a nonlinear regression model to obtain a radar water bloom distribution mask; and fusing the two masks to obtain a cyanobacterial bloom monitoring result. The application solves the problems that the existing cyanobacterial bloom detection method cannot realize all-weather monitoring, lacks cyanobacterial specificity recognition capability, and has insufficient reliability of a single data source, and improves the timeliness, accuracy and reliability of cyanobacterial bloom detection.

The invention belongs to the technical field of gene engineering, and particularly relates to a light-controlled yeast two-hybrid system as well as an establishment method and application thereof. The light-controlled yeast two-hybrid system is obtained by integrating a phycocyanobilin synthesis module into a yeast strain containing a reporter gene and then transferring into a phytochrome A-LexA fusion expression vector and an AD-FHY1 fusion expression vector. The light-controlled yeast two-hybrid system provided by the invention well overcomes the defects that the existing light-controlled yeast two-hybrid system needs exogenous application of phycocyanobilin, the operation is more complex and the cost is higher, and does not cause that the phytochrome apoprotein expressed in yeast cells cannot realize conversion of two conformational forms of Pfr / Pr; and finally, the difference between the interaction intensity of the phytochrome in two conformational forms and the FHY1 signal protein cannot be distinguished.

This invention discloses a method for preparing phycocyanin and its related applications, relating to the field of active substance extraction technology. Through a specific sequence of membrane filtration, cell disruption, and chromatography, this invention efficiently and selectively separates and purifies high-purity phycocyanin from the complex composition of *E. coli* fermentation broth. Compared to the cumbersome traditional process relying on algal extraction followed by chemical lysis, this method avoids the seasonality and geographical limitations of raw materials, achieving high recovery rates and highly selective enrichment of phycocyanin. The process flow is clear, the conditions are mild, and it is easy to scale up, demonstrating good industrial scalability potential. This invention not only provides a stable, economical, and efficient technical route for the large-scale preparation of phycocyanin but also lays a solid foundation for its in-depth application in high-value-added fields such as food, pharmaceuticals, and biological detection.

The invention discloses escherichia coli engineering bacteria with high yield of phycocyanobilin as well as a construction method and application of the escherichia coli engineering bacteria, and belongs to the technical field of synthetic biology. According to the engineering bacteria, escherichia coli BL21 (DE3) is taken as a host, heme oxygenase (HO) which is optimized by codons and is derived from Cornebacterium diphtheria and phycocyanobilin reductase (PcyA) which is derived from Synecocystis sp.PCC6803 are expressed, and a trxA dissolution promoting label is fused, so that the solubility and the stability of zymoprotein are effectively improved; meanwhile, a high-copy plasmid pRSFDuet is adopted for enhancing gene expression, a heme synthetase gene hemH, HO and PcyA are used for constructing a multi-enzyme complex through connecting peptides, precursor supply and enzyme space coordination are enhanced, and the synthesis efficiency of phycocyanobilin is remarkably improved. The yield of phycocyanobilin reaches up to 37 mg / L in shake flask fermentation, and the engineering bacterium is simple in process, low in cost and suitable for large-scale production.

The present disclosure addresses the problem of providing technology for preventing or improving aging-related hearing loss. This problem is solved by a composition for preventing or improving aging-related hearing loss, the composition comprising, as an active ingredient, at least one selected from the group consisting of spirulina, phycocyanin, and phycocyanobilin.

Disclosed is a method for producing phycocyanobilin by use of a recombinant Escherichia coli that express heterologous heme oxygenase ho1 and ferredoxin oxidoreductase pcyA derived from Synechocystis sp. PCC6803. According to the present disclosure, heterologous expression of ho1 and pcyA genes leads to conversion of heme to an intermediate biliverdin for phycocyanobilin synthesis, and reduces the accumulation of biliverdin in the process of the phycocyanobilin synthesis. The genome of E. coli is further engineered to overexpress related genes of a metabolic pathway of phycocyanobilin, and a strain of recombinant E. coli with high yield of phycocyanobilin is obtained. The recombinant E. coli strain is cultured for 36 hr in a system using glycerol as a substrate, and the phycocyanobilin yield can reach 147 mg / L.

The invention discloses a method for preparing oil-soluble phycocyanobilin through protease-lipase combination and an obtained product and application of the oil-soluble phycocyanobilin. The method comprises the steps that water-soluble phycocyanobilin is subjected to protease catalytic hydrolysis in a buffer solution, separation and purification are conducted after protease is inactivated, and a phycocyanobilin-peptide compound intermediate is obtained; dispersing the obtained intermediate in a non-polar organic solvent, catalyzing by using immobilized lipase, and carrying out esterification reaction with fatty alcohol with the chain length of C8 to C18, so as to obtain the oil-soluble phycocyanobilin derivative. According to the invention, water-soluble phycocyanin is successfully converted into oil-soluble phycocyanin, and the product has remarkable oil solubility and can be stably dispersed in a grease system; the reaction conditions are mild, the structure of the phycocyanobilin chromophore is effectively protected from being damaged, and the color and the biological activity of the product are ensured; the thermal stability of the modified phycocyanobilin at 60 DEG C is improved; the biological enzyme is used as the catalyst, the specificity is high, byproducts are few, the reaction solvent can be recycled, and the process is green and environment-friendly.

The invention belongs to the technical field of biology, and particularly relates to a photocatalytic biological enzyme and application thereof. The photocatalytic bio-enzyme is obtained through optimization and screening, and the photocatalytic bio-enzyme is obtained by catalyzing the fusion protein and phycocyanobilin through phycocyanin lyase. The photocatalytic biological enzyme and the preparation method are simple, rapid, green and environment-friendly, efficient catalytic reaction can be carried out under mild reaction conditions, and meanwhile, a new thought is provided for preparing clean energy by splitting water, reducing atmospheric carbon dioxide and relieving increasingly serious environmental pollution problems.