The invention relates to an enzyme-labeled antibody conjugate stabilizer and an application thereof. The enzyme-labeled antibody conjugate stabilizer contains rhamnolipid, dextran, mycose, cysteine, an alanine, glycine, lysine, threonine and sodium glutamate mixture, buffer salt powder with the pH value of 7.0-7.6, and water, and is used for protecting an enzyme-labeled antibody and improving the stability of the enzyme-labeled antibody in order to improve the stability and accuracy of enzyme-linked immunoassay. The enzyme-labeled antibody conjugate stabilizer prepared by adopting a biosurfactant used in the invention is novel, green and efficient, makes a enzyme-labeled antibody conjugate still have high activity in 2-8DEG C environment, obviously improves the stability of the enzyme-labeled antibody conjugate, improves the stability and accuracy of the enzyme-linked immunoassay, and has a very protection effect on other protein products.