Human proinsulin-KGD chimeric peptide as new recombinat antithrombotic with thrombocyte GP-IIb-IIIa receptor specificity

A technology of human insulin and chimeric peptides, which is applied in the direction of carrier binding/immobilizing peptides, medical preparations containing active ingredients, hybrid peptides, etc., to achieve the effects of no pollution to the environment, simple purification process, and high anti-platelet aggregation inhibitory activity
CN1810836AInactive Publication Date: 2006-08-02BEIJING NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BEIJING NORMAL UNIVERSITY
Publication Date
2006-08-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention obtains human proinsulin-KGD peptide protein molecule based on modern biochemical technology, by using the human proinsulin mutant, (CysA6Serú¼CysA11Serú¼DeltaB29- B30ú¼Delta A1)-human proinsulin, as molecular rack and through specific replacement with functional sequence CAKGDWNC containing Lys-Gly-Asp(KGD) in the original C peptide position. It is expressed effectively in pronucleus cell-colibacillus expression system, and the expressed product is purified through ultrasonic crushing, isoelectric precipitation, molecular sieve chromatographic separation, ion exchange chromatographic separation and other steps. The physical and chemical property analysis and the biological function detection show that the human proinsulin-KGD peptide protein has excellent molecular homogeneity, high GPIIb-IIIa acceptor recognizing and combining activity, powerful blood platelet coagulation inhibiting activity, no insulin hormone activity and no immunogenicity.
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Description

technical field

[0001] The invention relates to the field of biotechnology, more precisely, a novel antithrombotic agent developed based on modern genetic engineering technology and protein engineering downstream purification technology, and its design, preparation and application characteristics. Background technique

[0002] Proinsulin is the precursor protein of insulin. Insulin is a type of hormone that is normally secreted by the β-cells of the human pancreas. Its main function is to regulate the degradation and metabolism of blood sugar and promote the oxidation and decomposition of blood sugar. Therefore, it has the effect of reducing the glucose content in the blood. Under normal circumstances, the formation and degradation of glucose in the blood reach a dynamic balance and remain within a stable concentration range. When the secretion of insulin in the body is insufficient, or the body is desensitized to the insulin hormone due to pathological reasons, the glucose...

Claims

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