108 results about "C-peptide" patented technology

Methods for synthesizing proinsulin polypeptides are described that include a contacting a proinsulin polypeptide including an insulin polypeptide coupled to one or more peptides by peptide bond(s) capable of being cleaved to yield the insulin polypeptide with an oligomer under conditions sufficient to couple the oligomer to the insulin polypeptide portion of the proinsulin polypeptide and provide a proinsulin polypeptide-oligomer conjugate, and cleaving the one or more peptides from the proinsulin polypeptide-oligomer conjugate to provide the insulin polypeptide-oligomer conjugate. Methods of synthesizing proinsulin polypeptide-oligomer conjugates are also described as are proinsulin polypeptide-oligomer conjugates. Methods of synthesizing C-peptide polypeptide-oligomer conjugates are also described.

Peptide fragments of the p17 gag protein of HIV-1 from Clade C of from about 30 to about 50 amino acids, including the region extending from position 75 to position 129, raise antibodies recognizing other subtypes, including Clade A, Clade B, Clade E as well as peptides of non-coextensive but overlapping portions of the p17 gag protein. DNA sequences can be used to encode for the peptides of interest. An example of the Clade C peptide of this invention is a peptide having about 30 to about 32 amino acids taken consecutively from a part or the entirety of the following sequence: - (SEQ ID NO:2) - 5 10 - Ala Thr Leu Xaa Xaa Val His Xaa Xaa Ile Xaa Xaa Xaa - - 15 20 25 - Asp Thr Lys Glu Ala Leu Asp Xaa Xaa Xaa Glu Glu Gln - - 30 35 - Asn Lys Xaa Gln Gln Lys Xaa Xaa Thr - wherein Xaa at position 4 is Tyr or Trp; Xaa at position 5 is Cys or Ser; Xaa at position 8 is Lys, Glu, or Ala; Xaa at position 9 is Gly, Lys, Asp, Glu, or Arg; Xaa at position 11 is Glu; Xaa at position 12 is Val; Xaa at position 13 is Arg; Xaa at position 21 is Lys; Xaa at position 22 is Ile; Xaa at position 23 is Glu or Lys; Xaa at position 29 is Ile, Ser or Cys; Xaa at position 33 is a direct bond; and Xaa at position 34 is a direct bond.

Methods are described for determining the amount of insulin in a sample. Provided herein are mass spectrometric methods for detecting and quantifying insulin and C-peptide in a biological sample utilizing enrichment and / or purification methods coupled with tandem mass spectrometric or high resolution / high accuracy mass spectrometric techniques. Also provided herein are mass spectrometric methods for detecting and quantifying insulin and b-chain in a biological sample utilizing enrichment and / or purification methods coupled with tandem mass spectrometric or high resolution / high accuracy mass spectrometric techniques.