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Chemiluminescent examining method for insulin content and C-peptide content in human blood serum

A chemiluminescence detection and human serum technology, applied in the field of clinical blood immunodetection, can solve the problems of affecting the health and quality of life of patients, difficult to eliminate interference, inconvenient clinical use, etc., to achieve improved specificity, no radioactive pollution, and easy operation. Effect

Inactive Publication Date: 2007-05-16
AUTOBIO DIAGNOSTICS CO LTD
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Problems solved by technology

[0002] With the improvement of people's living standards and changes in lifestyle, the incidence of insulin dysfunction diseases is increasing day by day, resulting in a sharp increase in the incidence and number of diabetes. Due to the particularity of diabetes and its lesions, it seriously affects the patient's health Health and quality of life have become serious social problems. Currently, radioimmunoassay is mostly used for in vitro detection of insulin function. However, radioimmunoassay must use radioactive markers, and the detection equipment is complicated. Special radioactive detectors must be used for detection. As a result, radioactive pollution and injury to the operators also exist. At the same time, the commonly used iodine-125, iodine-131 and phosphorus-32 nuclides with short half-lives have a short storage period, which also brings inconvenience to clinical use. , which seriously limits the application of this project in clinical diagnosis
In the determination of insulin (INS) and C-peptide (C-P), the main performance is poor specificity, and it is difficult to rule out the interference of proinsulin and other insulin analogues

Method used

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  • Chemiluminescent examining method for insulin content and C-peptide content in human blood serum

Examples

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Embodiment

[0025] Example, (1) Preparation of a quantitative detection kit for human serum insulin:

[0026] 1. Coating plate production: use PH7.4 Tris-HCl buffer as the coating buffer, add 4ug / ml of monoclonal antibody, coat according to 100ul / well, 37°C for 3 hours and 4°C overnight, then shake off the liquid , Wash with washing solution, use 1% bovine serum albumin (BSA-PBS)-2.5% sucrose (0.01M, PH=7.4) as the blocking solution, and block overnight at 4°C.

[0027] 2. Antibody labeling:

[0028] 1) Dissolve 12 mg of horseradish peroxidase in 3 ml of freshly prepared deionized water.

[0029] 2) Add 0.1mol / L sodium periodate solution according to 51ul / 1mg enzyme, mix well, and activate at 4°C for 60 minutes.

[0030] 3) Add ethylene glycol according to 26ul / 1mg enzyme, mix well, react for 30 minutes at 4°C to terminate the reaction.

[0031] 4) Put the above reaction mixture into a dialysis bag, dialyzed against 1mmol / L acetic acid buffer, 4°C overnight, and change the dialysate 2-3 times...

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Abstract

This invention relates to one human serum insulin content and C-peptide chemical test method in clinical serum immune test technique, which uses human serum insulin quantitative test agent case to test human serum insulin content and human serum C-peptide agent case to test human serum C-peptide content.

Description

Technical field [0001] The invention belongs to the technical field of clinical blood immune detection methods, and relates to a method for clinically used for quantitative detection of insulin and C-peptide in human serum to determine diabetes. Background technique [0002] With the improvement of people’s living standards and lifestyle changes, the incidence of insulin dysfunction diseases is increasing, leading to a sharp increase in the incidence and number of diabetes. Due to the particularity of diabetes and its pathology, it has seriously affected patients’ health Health and quality of life have become a serious social problem. Currently, in vitro tests of insulin function mostly use radioimmunoassays. However, radioimmunoassays must use radioactive markers and the detection equipment is complex, so special radioactive detectors must be used for detection. The results are determined, and there is also radioactive contamination and damage to the operators. At the same time,...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N33/74G01N33/543G01N33/577G01N33/68G01N21/76
Inventor 付光宇李桂林李彬张学东马建军吴学炜
Owner AUTOBIO DIAGNOSTICS CO LTD
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