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Insulin and C peptide double-tagging determination kit

An insulin and double-labeling technology, which is applied in the field of insulin and C-peptide double-labeling kits, can solve the problems of poor stability and achieve high sensitivity, strong specificity, and wide detection range

Inactive Publication Date: 2012-04-18
GUANGZHOU DARUI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Insulin and C-peptide are less stable in serum, especially C-peptide

Method used

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  • Insulin and C peptide double-tagging determination kit
  • Insulin and C peptide double-tagging determination kit
  • Insulin and C peptide double-tagging determination kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The composition of embodiment 1 kit

[0044] 1) Calibrator dried blood film, 1 set, respectively numbered A-E

[0045] 2) Marker, 1 vial, freeze-dried

[0046] 3) 25× lotion, 1 bottle, 150mL

[0047] 4) Enhancement solution, 1 bottle, 150mL

[0048] 5) Assay buffer, 1 bottle, 150mL

[0049] 6) Coated reaction plate, 1 piece, 96 wells / block

[0050] 7) Sealed film, 3 pieces

[0051] 8) Manual, 1 copy

Embodiment 2

[0052] The usage method of embodiment 2 kit

[0053] 1. Sample collection

[0054] After local disinfection of the fingertips of the blood-collected person, puncture the blood-collecting needle to let the blood flow out naturally, wipe off the first drop of blood with a cotton swab or cotton ball, collect blood with the blood-collecting filter paper close to the bleeding site, and let the blood naturally penetrate to the back of the filter paper; sample collection Finally, the filter paper should be placed flat indoors to dry naturally, and stored at 2-8°C.

[0055] 2. Reagent preparation

[0056] (1) Washing liquid: Mix 50 mL of 25× washing liquid and 1200 mL of deionized water as the working washing liquid.

[0057] (2) Marker working solution: Dissolve each bottle of marker with 1 mL of deionized water one hour before use, and dilute 1:25 times with analysis buffer as the working solution.

[0058] 3. Operation steps

[0059] First take the coated reaction plate accordi...

Embodiment 3

[0060] Embodiment 3 compares the impact of buffer solution on the dissolution rate of filter paper dried blood slice

[0061] 1. Kit preparation

[0062] 1) The insulin and C-peptide double-labeled assay kit of Example 1 is referred to as Kit 1.

[0063] 2) Insulin AutoDELFIA kit (Cat. No.: B080-101) and C-peptide AutoDELFIA kit (Cat. No.: B081-101) from PerkinElmer, referred to as Kit 2 and Kit 3.

[0064] 2. The source of test samples

[0065] 24 cases of filter paper dried blood samples were collected from the hospital, numbered 201001-201024, the insulin concentration was between (10-150) μIU / ml, and the C-peptide concentration was between (3.0-18.6) ng / ml.

[0066] 3. Detection method

[0067] The analysis buffers in the above three kits were used respectively, and kit 1 was used to carry out parallel detection on 12 blood slices, and the measurement procedure and results were judged strictly according to Example 2.

[0068] 4. Test results

[0069] Table 1 Compariso...

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Abstract

The invention relates to an insulin and C peptide double-tagging kit, especially relates to a kit for determining insulin and C peptide by a double-tagging time resolution immunofluorescence and by using dried blood spots specimen on filter paper as a sample, and provides a preparation method of europium marked anti C peptide antibody and samarium marked insulin antibody. The kit can carry out simultaneous quantitative detections on two landmarks of insulin and C peptide through one detection, and the sample of dried blood spots specimen on filter paper solves a problem of unstable insulin and C peptide in a serum sample. The kit of the invention can be widely applied to various researching field of aided diagnosis and patient's condition detection, clinical drug guidance and prognosis of diabetes.

Description

technical field [0001] The present invention relates to a double-labeled kit for insulin and C-peptide, in particular to a kit for measuring insulin and C-peptide by using a double-labeled time-resolved immunofluorescence method with dried filter paper as a sample. Both insulin and C-peptide markers can be quantitatively detected at the same time. The application of the kit of the invention solves the problem of instability of insulin and C-peptide in serum samples, and can be widely used in multiple research fields such as auxiliary diagnosis and disease detection of diabetes, guidance of clinical medication and prognosis. Background technique [0002] Diabetes mellitus (DM) is a common and frequently-occurring disease, and it is also one of the three difficult diseases listed by the World Health Organization. How to prevent and treat DM is a major problem in modern medicine. DM is divided into type I (insulin-dependent) and type II ( Non-insulin-dependent), and its labora...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/577G01N21/64
Inventor 李明汤永平吴英松徐伟文朱坤
Owner GUANGZHOU DARUI BIOTECH
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