52 results about "Dried blood" patented technology

The utility model discloses a dried blood spot preparation device which comprises a sealing box, a cover body and a lining, a sample bin and a drying bin which are independent are arranged on the lining, a plurality of clamping grooves are formed in the sample bin and used for containing dried blood spot test paper, and a drying agent is arranged in the drying bin; a containing space is formed between the lining and the inner periphery of the sealing box, the containing space is communicated with the sample bin and the drying bin, and an air making system is arranged in the containing space. The dried blood spot preparation device can effectively promote quick drying of dried blood spots, so that water residues are reduced, the integrity of to-be-detected substances in the dried blood spots is guaranteed to the maximum extent, the stability of dried blood spot samples is remarkably improved, and a more reliable basis is provided for subsequent detection and analysis.

Analysis of nucleic acids, e.g cell-free DNA, extracted from a dried blood sample, preferably dried plasma or dried serum. The analysis may encompass detection of fragmentation patterns and / or sequence characteristics of cfDNA for detecting cancer.

The invention discloses an epidemic disease detection super-purification workbench for animal husbandry and veterinary medicine, and relates to the technical field of super-purification workbench cleaning. The animal husbandry veterinary epidemic disease detection super-purification workbench comprises a workbench body and a workbench top located in the workbench body, and further comprises an advancing device and a cleaning device, and the advancing device is used for driving the cleaning device to move in a reciprocating mode to clean the workbench top. The pressure of the scraping strip can be dynamically adjusted, the scraping rate of dry blood stains can be increased, the liquid spraying amount and the mixed concentration of a protease solution and clear water are automatically adjusted according to the cleaning level, the device adapts to stains of different degrees, the binding force of the stains and a table top is greatly weakened, assistance is provided for subsequent scraping of the scraping strip, and the working efficiency is improved. The cleaning effect of the working table top is effectively improved, the cleaning efficiency is improved, resource waste is avoided, and the use cost is reduced.

The application discloses a collecting device for quantitatively collecting dry blood spots, which comprises a sample collecting ring with both ends being permeable, a sample carrying sheet and a quantitative capillary for sampling, and the sample carrying sheet is detachably arranged in the sample collecting ring. The application also discloses a collecting method for collecting dry blood spots by using the collecting device. The application can quantitatively collect blood samples, and the sample can be directly sealed and sent without drying, punching and sampling, so as to reduce cross contamination, improve the accuracy of quantitative detection results of the dry blood spots, and make the quantitative collection, preservation, sample information input and sample sending operation of the sample be integrally completed by the device.

The present invention is directed to a method of preparing dehydrated blood products, comprising the steps of: (a) providing a hydrated blood product; (b) spray-drying the hydrated blood product to produce a dehydrated blood product, as well as dehydrated blood products made by the method. The present invention is directed to a method of treating a patient suffering from a blood-related disorder, comprising the steps of: (a) rehydrating a therapeutic amount of the dehydrated blood products to produce a rehydrated therapeutic composition; and (b) administering the rehydrated therapeutic composition to the patient. The present invention is directed to a bandage or surgical aid comprising the dehydrated blood products described above.

Newborn screening for primary immunodeficiency, cystine disease, and Wilson disease is described. Newborn screening enables the detection of these conditions from dried blood spots that have been conventionally collected at birth. Early detection of these conditions will greatly improve patient outcomes because each of these conditions may be fatal once a symptom occurs.

The invention belongs to the technical field of detection, and particularly discloses a filter paper dried blood spot thyroxine determination kit and a detection method thereof, and the filter paper dried blood spot thyroxine determination kit and the detection method provided by the invention are used for quantitative determination of neonatal thyroxine. Through the reconstitution fluid of the T4 antigen dry powder, the uniformity and the stability of manufacturing of the filter paper dried blood spot calibrator and the quality control product are realized; by optimizing antigen and antibody pairing, the detection sensitivity and precision of the reagent are effectively improved, and the linear range is widened; by optimizing a reaction carrier and a tracer agent process, the detection sensitivity of the reagent is improved, and the working range of a curve is widened; the elution release buffer solution is invented, so that elution, release and detection of TT4 in the filter paper dried blood spot are realized; and a full-automatic intelligent detection method is adopted, so that the detection speed, efficiency and precision are improved.

The invention relates to a sample carrier. The sample carrier has an absorbent film layer, which is based on a nonwoven polyolefin and has at least one absorption region with dried blood components. The sample carrier also has a plastic-based foil layer, wherein the foil layer is pretreated in an antistatic manner. The film layer and the foil layer are mechanically connected to one another in a subregion of the sample carrier, wherein the layers are otherwise not mechanically connected to one another in the absorption region. When the sample carrier is placed on a flat surface, the film layer preferably covers the foil layer at least in the absorption region.

The present invention discloses a simple dry blood tip quantitative blood sampling device, comprising: a hydrophilic fiber material, a tip, a pipette and a tip box; the hydrophilic fiber material is loaded in the tip 0.8 to 2.0 cm away from the tip's liquid aspiration end. The present invention further analyzes the dry blood tip sample to verify that the present invention has the ability to quantitatively collect and analyze. The blood sampling device of the present invention integrates online collection, storage, transportation and processing, reduces the risk of sample contamination, and improves the accuracy of the quantitative detection results of the dry blood tip; at the same time, it is easy to operate, and patients can complete self-sampling without going to the hospital for blood collection, which can reduce the gathering of people and the occurrence of infectious diseases.

Provided are methods and kits for blood sample collection, protein extraction, and analysis of neuro biomarkers obtained from blood samples on dried blood drop cards.

The invention relates to the technical field of assay and inspection, in particular to a method for detecting ceramide and phosphatidyl ethanolamine in neonatal dried blood spots, and the preparation method comprises the following steps: S1, drawing a standard curve equation: preparing an internal standard extract; preparing a mixed standard solution; preparing standard substance solutions with a series of concentrations; adding the internal standard extraction liquid into the standard substance solution, carrying out pretreatment, and carrying out HPLC-MS / MS detection to respectively obtain a ceramide standard curve and a phosphatidyl ethanolamine standard curve; and S2, detecting the contents of ceramide and phosphatidyl ethanolamine in the dried blood spot to be detected, namely adding the internal standard extract into the dried blood spot to be detected, performing pretreatment, performing HPLC-MS / MS detection, and obtaining the contents of ceramide and phosphatidyl ethanolamine according to a ceramide standard curve and a phosphatidyl ethanolamine standard curve respectively. The detection method provided by the embodiment of the invention has the characteristics of high accuracy, good sensitivity, stability and simplicity and convenience in operation.

The invention belongs to the technical field of biological detection, and particularly relates to a method for analyzing and detecting IgG N-glycan in dried blood spots. The dried blood spot sample is adopted to replace a traditional serum sample, so that the technical problem of IgG N-carbohydrate chain degradation under the normal-temperature storage condition of the serum sample is effectively solved, and the stability of the sample and the reliability of a detection result are remarkably improved. The dried blood spot sample collecting method is simple and convenient, vein blood sampling is not needed, the method is suitable for large-scale screening and application of special crowds, and the clinical application range of IgG N-glycan analysis is widened. Besides, the dried blood spot sample can be stored for a long time at room temperature, the transportation and storage cost is reduced, meanwhile, analysis can be completed only through a trace amount of blood, the method is suitable for repeated sampling and long-term monitoring, and a technical support is provided for research and application of IgG glycosylation as a biomarker.

A system for collecting menstrual blood and vaginal secretions for diagnostic and health monitoring purposes, and more particularly, to a system for providing absorbent materials, such as female sanitary pads and urinal pads, with a strip or patch for collecting bodily fluids, providing a container comprising a desiccant for transporting and storing the collected sample to a laboratory, dried menstrual blood samples (DMS), dried menstrual blood plasma samples (DMPS), dried blood spots (DBS), and / or dried blood plasma spots (DPS) are analyzed in a laboratory, or some of the containers without desiccant can maintain sufficient hydration to maintain the collected cells intact, for analysis using methods such as DNA testing, slide-based analysis, or cell culture, for example, for use in the field of laboratory analysis of dried menstrual blood samples (DMS), dried menstrual blood plasma samples (DMPS), dried blood spots (DBS), and / or dried blood plasma spots (DPS), or some of the containers without desiccant can maintain sufficient hydration to maintain the integrity of the collected cells. Various collection strip structures are provided that can be used for menstrual blood and vaginal secretions and for collecting and / or separating venous blood and capillary blood, as well as functional pad design elements to aid in sample collection, retention of collection strips, and to facilitate high speed manufacturing of pads.

The application provides a liquid chromatography-mass spectrometry method for detecting phytosterol content in dried blood spots, and belongs to the technical field of detection.The application simplifies a sampling process, uses a disposable blood taking needle and a blood taking card for sampling, replaces traditional serum or plasma samples with obtained dried blood spots, punches a sampling area of the dried blood spot sample through a puncher with a diameter of 3.2 mm, takes three dried blood spot samples, extracts seven kinds of sterol substances contained in the dried blood spot samples through n-hexane, adopts liquid chromatography-mass spectrometry, carries out detection and quantification through chromatographic separation and a mass spectrometry detector, realizes rapid and accurate determination of cholesterols, beta-sitosterols, daidzein, campesterol, dihydrocholesterol, dehydrocholesterol and 7-alkenyl cholestanol, and the method is simple in operation, accurate in quantification, and has a recovery rate of 92.4%-96.7%.

The invention discloses a nucleic acid release combination solution for dried blood spots, an extraction kit for genome DNA in the dried blood spots and an extraction method. The nucleic acid release combination solution comprises a cell dispersion solution and a cell lysis solution. The nucleic acid release combination liquid provided by the invention is applied to extraction of genomic DNA in dried blood spots, and the extraction amount of the genomic DNA can be effectively increased.

Provided are methods and kits for blood sample collection, protein extraction, and analysis of neuro biomarkers obtained from blood samples on dried blood drop cards.

The present invention provides methods, compositions, kits, and devices for detecting heavy metals in dried blood (e.g., dried blood spots). For example, the present invention provides: 1) dried blood spot paper that is detectably free of heavy metals and methods of preparing such paper using organic acid; 2) dried blood extraction solutions optimized for heavy metal detection (e.g., extraction solutions containing acetic acid and / or gold); 3) methods for estimating venous blood volume from dried blood mass; and 4) kits and kit components optimized for heavy metal detection in dried blood (e.g., kits with paper detectably free of heavy metals, heavy metal free skin wipes, metal free collection case, etc.).

The invention discloses a dry blood spot quantitative subpackaging device and method. The device comprises a base plate, a dried blood paper sheet and an operation switch. The substrate comprises a first surface and a second surface which are arranged back to back in a first direction. The substrate is provided with a groove and an open groove, the groove is arranged on the first surface or the second surface, and the open groove is arranged on the first surface or the second surface. A leading-in channel and a plurality of capillary channels are arranged in the base plate, each capillary channel has a preset volume, and the outlet end of each capillary channel is arranged on the bottom face of the groove. And the operation switch is movably connected in the open slot. The operation switch is used for switching the dry blood spot quantitative subpackaging device to a connection state and a disconnection state during operation. In the communication state, the introduction channel is communicated with the capillary channels. And the blocking state is that the introduction channel is disconnected from the plurality of capillary channels. The dried blood paper sheet is arranged in the groove and covers the outlet end of each capillary channel. The method can realize quantitative detection.

The invention relates to the field of medical waste recovery, in particular to medical protective article environment-friendly recovery treatment equipment which comprises a mounting table. The device further comprises a collecting box, a motor I, a winding roller, a mounting plate, a cutter and the like. A collecting box is fixedly connected to the mounting table; the collecting box is fixedly connected with a motor I; an output shaft of the motor I is fixedly connected with a winding roller; the collecting box is fixedly connected with a mounting plate; and the mounting plate is detachably connected with a cutter. An external water pump is controlled to input cleaning liquid into a connecting pipe through a liquid inlet pipe, finally, the cleaning liquid is sprayed into a cutter area in an infusion hose from a flow guide groove, then the cutting edge part and stabs of a cutter are cleaned in time, meanwhile, the cleaning liquid flushes the inner wall of the infusion hose, and the cleaning efficiency is improved. When the stab stamps and removes solidified blood attached to the inner wall of the infusion hose, the solidified and dried blood is moistened and softened, and the blood is convenient to remove.

Newborn screening for primary immunodeficiencies, cystinosis, and Wilson disease is described. The newborn screening can detect these disorders from dried blood spots already routinely collected at the time of birth. Early detection of these disorders will greatly improve patient outcome as each of them can be fatal once symptoms emerge.

The application discloses a quantitative dry blood sample collecting and preserving tube and a collecting method thereof, which comprises a liquid collecting mechanism, a liquid storage tube and a dry sealing mechanism. The top air bag is matched with the blood collecting tube. When the collecting liquid is discharged, the collecting liquid can be quickly discharged by extruding the top air bag, so that the efficiency of discharging the blood into the liquid storage tube is greatly improved. The dry sealing mechanism is separated from the liquid collecting mechanism, the exposure time of the drying agent in the drying tube is reduced, the whole structure is reasonable and ingenious, and the integrated structure is realized.

This invention belongs to the field of biomedical technology and discloses a DBS-LC-MS / MS method for the simultaneous detection of seven types of metabolites in dried blood spots. The method employs a pH / polarity dual-trigger microcapsule solvent carrier system, which internally encapsulates high, medium, and low polarity extraction solvents in layers. During extraction, these solvents are released sequentially according to local pH and polarity changes, efficiently extracting amino acids, acylcarnitines, organic acids, bile acids, steroids, fatty acids, and sugar metabolism intermediates, respectively. This invention overcomes the inherent limitations of traditional static solvent systems and solves the problem of unbalanced recovery rates caused by insufficient solvent compatibility in the extraction of seven types of metabolites with vastly different polarities in dried blood spots. It significantly improves the accuracy, reproducibility, and coverage of the DBS-LC-MS / MS platform in the simultaneous detection of multiple metabolites, providing reliable technical support for newborn screening for inherited metabolic diseases, diagnosis of endocrine diseases, and drug metabolism research.

The invention provides a solution for redissolving dried blood spots as well as a preparation method and application thereof, and belongs to the technical field of medical detection. The solution for redissolving the dried blood spots is a buffer solution containing the following components in percentage by mass: 0.1-10 mM of an antioxidant, 2%-10% of a protein stabilizer, 0.01%-1% of a surfactant and 0.1-5 mM of a metal ion chelating agent, the buffer pH value of the solution is 7.0 to 8.0. The solution protects the activity of an antibody by avoiding denaturation and degradation of the antibody in the dry blood spot redissolving process, so that the accuracy of a detection result is ensured, meanwhile, the dosage of a dry blood spot sample in an immune experiment is reduced, and the experiment cost and the operation difficulty are greatly reduced.

The present invention provides methods, compositions, kits, and devices for detecting heavy metals in dried blood (e.g., dried blood spots). For example, the present invention provides: 1) dried blood spot paper that is detectably free of heavy metals and methods of preparing such paper using organic acid; 2) dried blood extraction solutions optimized for heavy metal detection (e.g., extraction solutions containing acetic acid and / or gold); 3) methods for estimating venous blood volume from dried blood mass; and 4) kits and kit components optimized for heavy metal detection in dried blood (e.g., kits with paper detectably free of heavy metals, heavy metal free skin wipes, metal free collection case, etc.).

Newborn screening for primary immunodeficiency, cystinosis, and Wilson disease is described. Newborn screening can detect these conditions from dried blood spots that are already routinely collected at birth. Early detection of these conditions will greatly improve patient outcomes because each of these conditions can be fatal once symptoms develop.

Presented are methods, systems, and software products useful for determining the concentration of an analyte in a fluid specimen used to produce a dried sample, where the dried sample serves as a source of the analyte in a detection and quantification procedure. Particularly illustrated is the use of dried blood spots for quantifying a polynucleotide analyte.

This utility model relates to the field of blood dripping device technology, and discloses a blood genetic metabolic spectrum dried blood smear blood dripping auxiliary device, including a support, on which a sleeve is installed. A receiving groove is opened on one side of the sleeve for placing the barrel of a syringe. A bleeding head is installed at the bottom of the sleeve. After the limiting plate assembly is moved away from the top of the receiving groove, the barrel of the syringe is placed into the receiving groove, and then the limiting plate assembly is rotated to limit the movement distance of the core rod. At this time, the core rod of the syringe can be moved to squeeze the blood in the barrel, so that the blood drips onto the cardboard through the bleeding head. The limiting plate assembly can prevent excessive blood from dripping, increasing the accuracy of detection. Furthermore, the lifting and adjusting mechanism can drive the connecting plate assembly and the limiting plate assembly to move, thereby adjusting the position of the limiting plate assembly to drip different amounts of blood, solving the problem of not being able to accurately control the blood dripping volume in the prior art.

The utility model discloses a pig spray-dried blood cell powder stirring device, which belongs to the technical field of pig spray-dried blood cell powder preparation, and comprises a stirring barrel, a top cover is arranged above the stirring barrel, a motor is arranged above the top cover, the outer side of the stirring barrel is movably connected with a movable buckle handle, and the movable buckle handle is arranged on the top cover. A plum blossom button is in threaded connection with the upper portion of the movable buckle, a worm is movably connected to the conveying end of the motor, a worm gear is connected to one side of the worm in a meshed mode, a rotating shaft is movably connected to the lower portion of the worm gear, arc-shaped stirring blades are arranged on the outer side of the rotating shaft, and a connecting rod is installed on one side of each arc-shaped stirring blade; a mounting seat is arranged on one side of the connecting rod; the four groups of arc-shaped stirring blades are arranged on the outer side of the rotating shaft, the motor drives the worm and the worm gear to operate, the rotating shaft is used for driving the arc-shaped stirring blades to stir pig blood cell protein powder subjected to enzyme hydrolysis, the overall stirring area is large, stirring is more uniform, and the stirring effect is good.

Provided herein are methods for quantifying fCl-INH from dried blood spots. Such methods may include spotting and drying a blood sample on a support member, extracting protein from the dried blood sample, and measuring the level of fCl-INH in the extracted protein.