37 results about "Dried blood" patented technology

The utility model discloses a dried blood spot preparation device which comprises a sealing box, a cover body and a lining, a sample bin and a drying bin which are independent are arranged on the lining, a plurality of clamping grooves are formed in the sample bin and used for containing dried blood spot test paper, and a drying agent is arranged in the drying bin; a containing space is formed between the lining and the inner periphery of the sealing box, the containing space is communicated with the sample bin and the drying bin, and an air making system is arranged in the containing space. The dried blood spot preparation device can effectively promote quick drying of dried blood spots, so that water residues are reduced, the integrity of to-be-detected substances in the dried blood spots is guaranteed to the maximum extent, the stability of dried blood spot samples is remarkably improved, and a more reliable basis is provided for subsequent detection and analysis.

Analysis of nucleic acids, e.g cell-free DNA, extracted from a dried blood sample, preferably dried plasma or dried serum. The analysis may encompass detection of fragmentation patterns and / or sequence characteristics of cfDNA for detecting cancer.

The invention discloses an epidemic disease detection super-purification workbench for animal husbandry and veterinary medicine, and relates to the technical field of super-purification workbench cleaning. The animal husbandry veterinary epidemic disease detection super-purification workbench comprises a workbench body and a workbench top located in the workbench body, and further comprises an advancing device and a cleaning device, and the advancing device is used for driving the cleaning device to move in a reciprocating mode to clean the workbench top. The pressure of the scraping strip can be dynamically adjusted, the scraping rate of dry blood stains can be increased, the liquid spraying amount and the mixed concentration of a protease solution and clear water are automatically adjusted according to the cleaning level, the device adapts to stains of different degrees, the binding force of the stains and a table top is greatly weakened, assistance is provided for subsequent scraping of the scraping strip, and the working efficiency is improved. The cleaning effect of the working table top is effectively improved, the cleaning efficiency is improved, resource waste is avoided, and the use cost is reduced.

The application discloses a collecting device for quantitatively collecting dry blood spots, which comprises a sample collecting ring with both ends being permeable, a sample carrying sheet and a quantitative capillary for sampling, and the sample carrying sheet is detachably arranged in the sample collecting ring. The application also discloses a collecting method for collecting dry blood spots by using the collecting device. The application can quantitatively collect blood samples, and the sample can be directly sealed and sent without drying, punching and sampling, so as to reduce cross contamination, improve the accuracy of quantitative detection results of the dry blood spots, and make the quantitative collection, preservation, sample information input and sample sending operation of the sample be integrally completed by the device.

The present invention is directed to a method of preparing dehydrated blood products, comprising the steps of: (a) providing a hydrated blood product; (b) spray-drying the hydrated blood product to produce a dehydrated blood product, as well as dehydrated blood products made by the method. The present invention is directed to a method of treating a patient suffering from a blood-related disorder, comprising the steps of: (a) rehydrating a therapeutic amount of the dehydrated blood products to produce a rehydrated therapeutic composition; and (b) administering the rehydrated therapeutic composition to the patient. The present invention is directed to a bandage or surgical aid comprising the dehydrated blood products described above.

Newborn screening for primary immunodeficiency, cystine disease, and Wilson disease is described. Newborn screening enables the detection of these conditions from dried blood spots that have been conventionally collected at birth. Early detection of these conditions will greatly improve patient outcomes because each of these conditions may be fatal once a symptom occurs.

The invention belongs to the technical field of detection, and particularly discloses a filter paper dried blood spot thyroxine determination kit and a detection method thereof, and the filter paper dried blood spot thyroxine determination kit and the detection method provided by the invention are used for quantitative determination of neonatal thyroxine. Through the reconstitution fluid of the T4 antigen dry powder, the uniformity and the stability of manufacturing of the filter paper dried blood spot calibrator and the quality control product are realized; by optimizing antigen and antibody pairing, the detection sensitivity and precision of the reagent are effectively improved, and the linear range is widened; by optimizing a reaction carrier and a tracer agent process, the detection sensitivity of the reagent is improved, and the working range of a curve is widened; the elution release buffer solution is invented, so that elution, release and detection of TT4 in the filter paper dried blood spot are realized; and a full-automatic intelligent detection method is adopted, so that the detection speed, efficiency and precision are improved.

The invention relates to the technical field of assay and inspection, in particular to a method for detecting ceramide and phosphatidyl ethanolamine in neonatal dried blood spots, and the preparation method comprises the following steps: S1, drawing a standard curve equation: preparing an internal standard extract; preparing a mixed standard solution; preparing standard substance solutions with a series of concentrations; adding the internal standard extraction liquid into the standard substance solution, carrying out pretreatment, and carrying out HPLC-MS / MS detection to respectively obtain a ceramide standard curve and a phosphatidyl ethanolamine standard curve; and S2, detecting the contents of ceramide and phosphatidyl ethanolamine in the dried blood spot to be detected, namely adding the internal standard extract into the dried blood spot to be detected, performing pretreatment, performing HPLC-MS / MS detection, and obtaining the contents of ceramide and phosphatidyl ethanolamine according to a ceramide standard curve and a phosphatidyl ethanolamine standard curve respectively. The detection method provided by the embodiment of the invention has the characteristics of high accuracy, good sensitivity, stability and simplicity and convenience in operation.

The invention belongs to the technical field of biological detection, and particularly relates to a method for analyzing and detecting IgG N-glycan in dried blood spots. The dried blood spot sample is adopted to replace a traditional serum sample, so that the technical problem of IgG N-carbohydrate chain degradation under the normal-temperature storage condition of the serum sample is effectively solved, and the stability of the sample and the reliability of a detection result are remarkably improved. The dried blood spot sample collecting method is simple and convenient, vein blood sampling is not needed, the method is suitable for large-scale screening and application of special crowds, and the clinical application range of IgG N-glycan analysis is widened. Besides, the dried blood spot sample can be stored for a long time at room temperature, the transportation and storage cost is reduced, meanwhile, analysis can be completed only through a trace amount of blood, the method is suitable for repeated sampling and long-term monitoring, and a technical support is provided for research and application of IgG glycosylation as a biomarker.

A system for collecting menstrual blood and vaginal secretions for diagnostic and health monitoring purposes, and more particularly, to a system for providing absorbent materials, such as female sanitary pads and urinal pads, with a strip or patch for collecting bodily fluids, providing a container comprising a desiccant for transporting and storing the collected sample to a laboratory, dried menstrual blood samples (DMS), dried menstrual blood plasma samples (DMPS), dried blood spots (DBS), and / or dried blood plasma spots (DPS) are analyzed in a laboratory, or some of the containers without desiccant can maintain sufficient hydration to maintain the collected cells intact, for analysis using methods such as DNA testing, slide-based analysis, or cell culture, for example, for use in the field of laboratory analysis of dried menstrual blood samples (DMS), dried menstrual blood plasma samples (DMPS), dried blood spots (DBS), and / or dried blood plasma spots (DPS), or some of the containers without desiccant can maintain sufficient hydration to maintain the integrity of the collected cells. Various collection strip structures are provided that can be used for menstrual blood and vaginal secretions and for collecting and / or separating venous blood and capillary blood, as well as functional pad design elements to aid in sample collection, retention of collection strips, and to facilitate high speed manufacturing of pads.

The application provides a liquid chromatography-mass spectrometry method for detecting phytosterol content in dried blood spots, and belongs to the technical field of detection.The application simplifies a sampling process, uses a disposable blood taking needle and a blood taking card for sampling, replaces traditional serum or plasma samples with obtained dried blood spots, punches a sampling area of the dried blood spot sample through a puncher with a diameter of 3.2 mm, takes three dried blood spot samples, extracts seven kinds of sterol substances contained in the dried blood spot samples through n-hexane, adopts liquid chromatography-mass spectrometry, carries out detection and quantification through chromatographic separation and a mass spectrometry detector, realizes rapid and accurate determination of cholesterols, beta-sitosterols, daidzein, campesterol, dihydrocholesterol, dehydrocholesterol and 7-alkenyl cholestanol, and the method is simple in operation, accurate in quantification, and has a recovery rate of 92.4%-96.7%.

Provided are methods and kits for blood sample collection, protein extraction, and analysis of neuro biomarkers obtained from blood samples on dried blood drop cards.

The present invention provides methods, compositions, kits, and devices for detecting heavy metals in dried blood (e.g., dried blood spots). For example, the present invention provides: 1) dried blood spot paper that is detectably free of heavy metals and methods of preparing such paper using organic acid; 2) dried blood extraction solutions optimized for heavy metal detection (e.g., extraction solutions containing acetic acid and / or gold); 3) methods for estimating venous blood volume from dried blood mass; and 4) kits and kit components optimized for heavy metal detection in dried blood (e.g., kits with paper detectably free of heavy metals, heavy metal free skin wipes, metal free collection case, etc.).

The application discloses a quantitative dry blood sample collecting and preserving tube and a collecting method thereof, which comprises a liquid collecting mechanism, a liquid storage tube and a dry sealing mechanism. The top air bag is matched with the blood collecting tube. When the collecting liquid is discharged, the collecting liquid can be quickly discharged by extruding the top air bag, so that the efficiency of discharging the blood into the liquid storage tube is greatly improved. The dry sealing mechanism is separated from the liquid collecting mechanism, the exposure time of the drying agent in the drying tube is reduced, the whole structure is reasonable and ingenious, and the integrated structure is realized.

This invention belongs to the field of biomedical technology and discloses a DBS-LC-MS / MS method for the simultaneous detection of seven types of metabolites in dried blood spots. The method employs a pH / polarity dual-trigger microcapsule solvent carrier system, which internally encapsulates high, medium, and low polarity extraction solvents in layers. During extraction, these solvents are released sequentially according to local pH and polarity changes, efficiently extracting amino acids, acylcarnitines, organic acids, bile acids, steroids, fatty acids, and sugar metabolism intermediates, respectively. This invention overcomes the inherent limitations of traditional static solvent systems and solves the problem of unbalanced recovery rates caused by insufficient solvent compatibility in the extraction of seven types of metabolites with vastly different polarities in dried blood spots. It significantly improves the accuracy, reproducibility, and coverage of the DBS-LC-MS / MS platform in the simultaneous detection of multiple metabolites, providing reliable technical support for newborn screening for inherited metabolic diseases, diagnosis of endocrine diseases, and drug metabolism research.

The invention provides a solution for redissolving dried blood spots as well as a preparation method and application thereof, and belongs to the technical field of medical detection. The solution for redissolving the dried blood spots is a buffer solution containing the following components in percentage by mass: 0.1-10 mM of an antioxidant, 2%-10% of a protein stabilizer, 0.01%-1% of a surfactant and 0.1-5 mM of a metal ion chelating agent, the buffer pH value of the solution is 7.0 to 8.0. The solution protects the activity of an antibody by avoiding denaturation and degradation of the antibody in the dry blood spot redissolving process, so that the accuracy of a detection result is ensured, meanwhile, the dosage of a dry blood spot sample in an immune experiment is reduced, and the experiment cost and the operation difficulty are greatly reduced.

The present invention provides methods, compositions, kits, and devices for detecting heavy metals in dried blood (e.g., dried blood spots). For example, the present invention provides: 1) dried blood spot paper that is detectably free of heavy metals and methods of preparing such paper using organic acid; 2) dried blood extraction solutions optimized for heavy metal detection (e.g., extraction solutions containing acetic acid and / or gold); 3) methods for estimating venous blood volume from dried blood mass; and 4) kits and kit components optimized for heavy metal detection in dried blood (e.g., kits with paper detectably free of heavy metals, heavy metal free skin wipes, metal free collection case, etc.).

Newborn screening for primary immunodeficiency, cystinosis, and Wilson disease is described. Newborn screening can detect these conditions from dried blood spots that are already routinely collected at birth. Early detection of these conditions will greatly improve patient outcomes because each of these conditions can be fatal once symptoms develop.

This utility model relates to the field of blood dripping device technology, and discloses a blood genetic metabolic spectrum dried blood smear blood dripping auxiliary device, including a support, on which a sleeve is installed. A receiving groove is opened on one side of the sleeve for placing the barrel of a syringe. A bleeding head is installed at the bottom of the sleeve. After the limiting plate assembly is moved away from the top of the receiving groove, the barrel of the syringe is placed into the receiving groove, and then the limiting plate assembly is rotated to limit the movement distance of the core rod. At this time, the core rod of the syringe can be moved to squeeze the blood in the barrel, so that the blood drips onto the cardboard through the bleeding head. The limiting plate assembly can prevent excessive blood from dripping, increasing the accuracy of detection. Furthermore, the lifting and adjusting mechanism can drive the connecting plate assembly and the limiting plate assembly to move, thereby adjusting the position of the limiting plate assembly to drip different amounts of blood, solving the problem of not being able to accurately control the blood dripping volume in the prior art.

The invention relates to the field of environmental chemistry and biological sample analysis, in particular to a kit for detecting bisphenol compounds in dried blood spots and a detection method. The kit comprises a mixed standard stock solution and a mixed internal standard stock solution, and can be used for simultaneously detecting bisphenol compounds at least comprising BPA, BPF, BPS, BPAF, BPAP, BPB, BPC, 2, 4-BPS, BPSIP, BPS-MAE, TGSA, DPS, DDS, TDP, D-90 and DD-70 in dried blood spots. The kit and the detection method disclosed by the invention have the advantages of high absolute recovery rate, low matrix effect, high sensitivity, high accuracy, high stability and the like, and are used as a first report for simultaneously detecting various bisphenol compounds in dried blood spots; the method has wide practical application value and significance for evaluating the health effect of the bisphenol compound and the health condition of a human body.

The utility model provides a puncher with a sample collecting structure, which comprises a punching equipment body and a sample placing component, and the sample placing component used for placing a dried blood filter paper sample is arranged on the left side in the punching equipment body. Compared with the prior art, the dry blood filter paper sample collecting device has the advantages that the punching device body is arranged to be matched with the sample containing assembly, workers do not need to stand for all the time, and therefore the work efficiency is improved, and the labor intensity of workers is reduced. The labor intensity of operators can be reduced, the operators do not need to watch in front of the equipment all the time, a large number of samples can be treated, and the treated filter paper samples are collected into the corresponding sorted collection barrels according to the sequence by arranging the liftable collection assembly, so that subsequent sample operation is facilitated, and the working efficiency is improved. And the equipment can deal with large-batch sample processing work.

The invention relates to a blank substitute matrix for preparing dried blood spots and a preparation method of the blank substitute matrix, and belongs to the technical field of medical biological detection.The blank substitute matrix comprises a biological matrix which is a high polymer material, animal protein and / or plant polysaccharide; a toner; and a solvent. The method provided by the invention can be used for preparing the blank substitute matrix of the dried blood spot simply and safely at low cost, and the blank substitute matrix for preparing the dried blood spot has no biological hazard and good reproducibility.

The invention discloses a method and a kit for detecting five immunosuppressants in dried blood spots, and the method comprises the following steps: step 1, taking pig whole blood as a blank whole blood matrix, sucking whole blood drops onto filter paper, airing at room temperature, sealing, and storing for later use; step 2, taking dried blood spots, adding water, a precipitant solution and an internal standard mixed solution, performing vortex mixing uniformly, performing centrifugation, and taking supernate; and step 3, carrying out liquid chromatography-tandem mass spectrometry sample injection detection on the supernate. According to the method disclosed by the invention, treatment drug monitoring can be simultaneously carried out on five immunosuppressants which are commonly used, so that more complete treatment drug use information can be obtained in time, and an individualized drug administration scheme can be conveniently adjusted; the pretreatment method is simple and convenient, and used reagents are safe and harmless; the blood collection amount is small, the wound surface is small, only 20 microliters of peripheral blood is needed for one dried blood spot, the difficulty of multiple times of detection and blood collection can be solved, and the dried blood spot is easier to store and transport compared with whole blood.

The application provides a solution for reconstituting dry blood spots and a preparation method and application thereof, and belongs to the technical field of medical detection. The solution for reconstituting dry blood spots is a buffer solution containing the following components: 0.1-10 mM antioxidant, 2%-10% protein stabilizer in terms of mass percentage, 0.01%-1% surfactant in terms of mass percentage and 0.1-5 mM metal ion chelating agent; and the buffer pH value of the solution is 7.0-8.0. In the reconstitution process of the dry blood spots, the solution avoids the denaturation and degradation of antibodies, protects the activity of the antibodies, thereby ensuring the accuracy of the detection results, reducing the amount of dry blood spot samples in the immunological experiment, and greatly reducing the experimental cost and operation difficulty.

The invention relates to the technical field of assay and inspection, in particular to a detection kit for ceramide and phosphatidyl ethanolamine in neonatal dried blood spots and a use method of the detection kit, and the detection kit comprises a calibration product, a dried blood spot quality control product, an internal standard stock solution, an extracting solution, a mobile phase additive and a plurality of microwell plates. The detection kit and the use method thereof have the characteristics of high accuracy, good sensitivity, stability and simplicity and convenience in operation.

This invention relates to the field of automated detection technology, specifically to an optimized disposable adhesive pin and automated punching method and system for dried blood smear sample collection. The method includes the following steps: acquiring the disc number sequence and status code to construct a status vector; comparing and generating an insertion docking table; extracting punching coordinates and correcting offsets; binding parameters to construct a cutting command; controlling the cutting and identifying channel coding features; acquiring displacement and performing consistency judgment to form a completion mark; updating the status vector and remapping empty space relationships. In this invention, by real-time recognition of the dried blood spot disc barcode image and comparison of the coding vector, a one-to-one match between the disc and empty space states is achieved. Coordinate difference correction is completed before the insertion action, effectively avoiding the accumulation of cutting position errors, effectively avoiding the problem of duplicate or missing sample information insertion, improving overall insertion efficiency and accurate traceability, and ensuring the reliability and continuity of automated processing of large batches of samples.

This invention discloses a method for quantitatively detecting sunitinib (SUN) and N-deethylsunitinib (SU12662) in dried blood spots and its application. This invention employs quantitative dried blood spot (qDBS) sampling combined with LC-MS / MS detection. By using fixed-volume sample loading and whole-spot sampling, hematocrit interference is eliminated. Under specific chromatographic and mass spectrometric conditions, precise quantification of SUN and SU12662 in dried blood spots is achieved. Simultaneously, this invention establishes a dried blood spot-plasma concentration conversion model, converting whole blood concentrations to clinically applicable plasma concentrations, forming a home sampling protocol for sunitinib treatment monitoring. This method requires only 15 μL of fingertip blood, the sample is stable and easy to store and transport, exhibits good linearity, high sensitivity, and minimal matrix effect, and can tolerate 30%–50% hematocrit fluctuations. It allows for home self-collection, is non-invasive and convenient, and the results are highly consistent with clinical venous blood collection, effectively improving patient compliance and accessibility to treatment monitoring. It is suitable for personalized sunitinib medication guidance.

Newborn screening for primary immunodeficiencies, cystinosis, and Wilson disease is described. The newborn screening can detect these disorders from dried blood spots already routinely collected at the time of birth. Early detection of these disorders will greatly improve patient outcome as each of them can be fatal once symptoms emerge.

The invention belongs to the field of blood product preparation and preservation, and discloses a processing method and system for improving the stability of a blood product, and the processing method comprises the following steps: starting a vacuum system, and rapidly pumping air by a vacuum pump until the pressure is stabilized within 50-100 Pa; starting the pulsed electric field module, applying an electric field with the intensity of 500-1500V / m, and cooperating with 25kHz ultrasonic waves generated by the ultrasonic transducer to jointly act on the blood product; real-time monitoring is performed through a multi-dimensional temperature and humidity sensor and a vacuum sensor; a PID closed-loop control algorithm is adopted, and according to real-time temperature and humidity and vacuum data, parameters such as the pumping speed, the electric field intensity and the ultrasonic intensity of a vacuum pump are accurately adjusted in real time; and after the drying reaches a set moisture content threshold value, the system automatically stops heating, ultrasonic and electric field actions, the drying process is finished, and a stable and uniform low-temperature dried blood product is obtained. The drying effect and the quality stability of the blood product are greatly improved, the production cost is reduced, and good clinical and industrial popularization and application prospects are achieved.

Management of the health status of an animal colony using a plurality of blood collection cards and the analysis of dried blood from members of the colony that has been collected on the cards. Members of the colony may be removed from the colony as a result of the analysis.