268 results about "Dried blood" patented technology

This invention relates to the measurement of analytes in dried blood samples. It has been discovered by measuring the hemoglobin in the dissolved dried blood sample, an estimate of blood volume may be made and used to make a correction for blood volume. Thus, analytes such as cholesterol, PSA, and other blood analytes can be more accurately determined. The invention further describes a blood collection device and disk material on which blood is dried to provide superior results.

Pipette tips, pipetting system and methods for eluting an analyte from a solid phase filtration media that resides within an upper portion of the pipette tips. Each pipette tip includes an upper barrel containing and retaining the solid phase filtration media and a lower cannula. The solid phase filtration media resides between the upper barrel and the lower cannula. An eluting solvent is provided into the pipette tips for eluting the analyte from the solid phase filtration media by soaking, ultrasonic agitation or agitation by mixing. A sorbent material may reside within the cannula portion of each pipette tip for filtering or treating the eluted analyte containing solution prior to it being dispensed from the pipette tip into a microplate for subsequent analytical processing.

The invention discloses a method for simultaneously detecting forty types of amino acids in dried blood spots, blood and urine. The method comprises the following steps of firstly, pretreating a biological sample by a simple liquid and liquid extracting method; performing chromatographic separation and mass spectrometric detection; using the relative retention time and qualitative ions as the qualitative basis for each type of amino acid, and using a standard product to manufacture a standard curve for quantifying. The method has the advantages that the accuracy and validity of the method shall be studied by three levels of quality control products, so as to avoid the distortion of detection results; the forty types of non-derived amino acids in one sample can be simultaneously detected bya HPLC-MS/MS (high performance liquid chromatography-mass spectrograph) technique for the first time; the operation is simple, convenient and rapid, the flux is high, and the cost is low; the level of amino acids in a human body can be effectively monitored in real time; the enough basis is provided for the clinical disease diagnosis; the method is suitable for being clinically popularized and applied.

The invention discloses a personalized medication gene detection kit and application. The personalized medication gene detection kit is suitable for multiple kinds of sample types including dried blood spots, whole blood and buccal swabs, information of all medicine gene polymorphic sites can be acquired at once, the sequencing effective rate is 50% or above, the sequencing cover degree is 100%, the average sequencing depth exceeds 3000 X, the library building success rate is larger than or equal to 98.5%, and the medicine gene polymorphic site detection accuracy is larger than or equal to 99.9%; a primer group is high in specificity and little in nonspecific amplification, interference among primers can be well overcome, under the situation of up to 186 primer sequences, one-tube multiplePCR reaction is achieved, moreover, amplicon is high in uniformity, and under the situation of large amplification area GC content difference, overall effective amplification can be conducted. The detection kit, the primer group and a library building method can be applied to personalized medication gene detection, and guidance is provided for personalized medication.

The invention relates to a liquid chromatogram tandem mass spectrum detection method for 25-hydroxy vitamin D in a dry blood sample. The pretreatment technique mainly comprises the processes of sample pretreatment, deriving and redissolving, and meanwhile, the multi-reaction monitoring MRM scanning mode is adopted. According to the method, the high-flux liquid chromatogram tandem mass spectrum detection method for 25-hydroxy vitamin D in the dry blood sample can be realized; the problems of large demanded quantity of samples and inconvenience in sampling and transporting of the traditional liquid chromatogram tandem mass spectrum method are solved; through the improvement for the detection technique, the detection for the 25-hydroxy vitamin D in the dry blood sample is realized, the detector can voluntarily collect at home, the demanded quantity of samples is small and the sampling and transporting are convenient.

An apparatus for sampling and storing biological fluids from a human or animal subject is provided. In one embodiment of the present disclosure, the apparatus includes a main body, lancet carrier or hub, lancet, lancet trigger, capillary tube, and sample compartments for collecting and storing dried blood and other bodily fluids. The lancet hub supports a lancet and provides for moving the lancet longitudinally between a first retracted position and a second extended position. The device includes a capillary tube having an internal diameter sized to draw and retain fluid from a contacted source using capillary action. The main body of the apparatus further includes a sample compartment for holding sampling and storage materials. In at least one embodiment of the present disclosure, the sample compartment can be accessed by lifting sample compartment lid. Also included is a new “fan” or “daisy” shaped collection material format for use in collecting and preserving samples.

The invention discloses a traditional Chinese medicament preparation for treating various types of skin diseases caused by dampness, heat, dry blood and unsmooth venation. A preparation method for the traditional Chinese medicament preparation comprises the following steps: taking long-noded pit viper, indigo naturalis, clamshell, madder, peach seed, schizonepeta spike carbon, wine roasted snake slough, red peony, angelica sinensis, lalang grass rhizome, kochia scoparia, fried xanthium fruit, dried rehmannia root, prepared rehmannia root, fructus forsythiae, honeysuckle, bunge corydalis herb, polygonum cuspidatum, rhizoma smilacis glabrae, golden cypress, spina gleditsiae, platycodon grandiflorum, leonurus, bitter apricot kernel (peeled and fried), divaricate saposhnikovia root, red Tuckahoe, white paeony root, cicada slough, fried burdock, moutan bark, dictamnus dasycarpus turcz, rhubarb fried by wine, caulis lonicerae, lithospermum, rhizoma bolbostemmae,wine roasted ligusticum wallichii, liquorice, black soya bean, radix angelicae dahuricae, muskroot-like semiaquilegia root, bark of chinese redbud, suberect spatholobus stem, lotus leaf, serissa serissoides, duckweed, wine roasted radix rubi parvifolii, safflower and talcum powder; floating pills by water; adding the talcum powder to serve as a coating; adding glutinous rice juice or a suitable amount of maltose juice adhesive. The previous experience prescriptions are inherited and integrated technologically, and the synthesis and innovation are performed after technology integration is performed.

The invention provides a method for joint detection of metabolic markers based on ultra high performance liquid chromatography-tandem mass spectrometry. Qualitative and quantitative analysis of more than 134 metabolites including amino acids and carnitines is completed only through one-time detection of only an extremely low amount, being 3 [mu]L, of a blood sample by adopting an isotope-labeled amino acid standard substance and an isotope-labeled carnitine standard substance as internal standard substances, a high-concentration quality control blood tablet and a low-concentration quality control blood sheet as positive control substances and an acetonitrile-water solution as a mobile phase through reasonably setting parent ion scanning, neutral loss scanning and multi-reaction ion monitoring scanning combined ultra performance liquid chromatograph-tandem mass spectrometer detection conditions. The method has the advantages of small sample volume, high sensitivity, high accuracy, highthroughput and high repeatability, and is suitable for large-scale analysis of clinical dried blood tablet samples.

The invention discloses a grass carp formula feed prepared from the following raw materials by weight: 30-40 parts of soybean meal, 20-30 parts of sorghum flour, 10-15 parts of bone powder, 6-8 parts of garlic, 6-8 parts of pumpkin flower, 5-6 parts of loquat leaf, 5-6 parts of pine needle powder, 2-3 parts of tenebrio molitor powder, 3-4 parts of radix trichosanthis, 5-6 parts of dried blood, 2-3 parts of chestnut root, 1-2 parts of myrcia, 3-4 parts of pomegranate seed, 3-4 parts of jujube tree skin, 1-2 parts of shea butter, 1-2 parts salt, 3-5 parts of a phagostimulant and a proper amount of water; the grass carp formula feed effectively mixing a variety of components is scientific and reasonable in formula, can meet the needs of various stages of grass carp growth, is high in feed utilization and good in palatability, can effectively improve the meat quality and nutritional value of grass carp, promotes the growth of grass carp, and can also improve the sick rate and survival rate of the grass carp. In addition, the grass carp formula feed is less in water body pollution and low in cost, and has good environmental and economic benefits.

The invention discloses a blood cake specimen collecting and treating method and a collection card dry blood cake DNA (deoxyribose nucleic acid) extracting method thereof. The blood cake specimen collecting and treating method includes steps of (1), collecting blood; (2), treating collection card blood cakes; (3), preserving the collection card dry blood cakes; and (4), transferring the collection card dry blood cakes. The blood cake specimens can be preserved at normal temperature, easy to transport, convenient to deliver for examination, and can substitute for anti-coagulation blood as DNA extracting specimen for genetic diagnosis.

The invention provides a kit for quantitatively detecting niacin, nicotinamide and pantothenic acid in peripheral whole blood at the same time, which mainly consists of dried blood slices (Whatman protein saver 903), capillary blood collection tubes, nicotinic acid and nicotinamide and pantothenic acid vitamin standard substance and its isotope internal standard, trichloroacetic acid, and also includes dry blood spot preparation, dry blood spot pretreatment and detection steps. The nicotinic acid, nicotinamide, and pantothenic acid dry blood spot quantitative kit provided by the present invention combines dry blood spot sampling technology with high performance liquid chromatography-tandem mass spectrometry technology for the quantification of B vitamins, a minimally invasive, minimal blood collection at the same time Realize the evaluation of the nutritional status of multiple B vitamins, and realize the quantitative work of simultaneous detection of multiple vitamins under low sample volume conditions that cannot be accomplished by metabolic enzyme-dependent methods and immunoassay methods.

The invention discloses an enzymolysis processing method of whole blood polypeptide albumen powder. In the method, livestock and poultry blood serve as a raw material, proper primer density, effective albumen enzymolysis complex enzyme and reductant and enzymolysis reaction temperature are chosen to cause livestock and poultry blood to be performed with enzymolysis reaction, reduction reaction and low-temperature drying and smashing to obtain the whole blood polypeptide albumen powder. The enzymolysis processing method degrades large-molecular albumen hard to adsorb and utilize in the livestock and poultry blood into small-molecular albumen, polypeptide and free amino acid easy to digest and adsorb, and improves the palatability and the nutritional value of dried blood for feeding. The processing method has small investment, low cost, short reaction time and high production efficiency. The whole blood polypeptide albumen powder obtained by the processing method can lower feed formulation cost if added by 1-5% in animal diet, improve feed compensation, enhance daily gain andresistance to disease and obtain favourable economic benefit.

Described are reagents, methods, and kits for eluting, and amplifying and/or characterizing DNA from liquid and dried blood samples. A one-step DNA elution buffer has been developed that simplifies purification of DNA from blood samples. The purified DNA is suitable for use in subsequent widely used techniques such as enzymatic DNA amplification and quantitative analysis such as real-time PCR.

The invention belongs to the technical field of gene engineering and discloses a composition of primers and probes for detecting polymorphism of human CYP2C19 gene, a kit containing the composition of the primers and the probes, and a fluorescence PCR method for detecting the polymorphism of the human CYP2C19 gene by using the composition of the primers and the probes or the kit. Based on a TaqMan fluorescence PCR technology, the primers, the probes and the kit are simple and rapid, and are high in sensitivity; in addition, through reasonable collocation of the primers and the probes, the interaction between the primers, the interaction between the primers and the probes and the interaction between the probes can be effectively avoided; the detection errors are reduced. When the primers, the probes and the kit, provided by the invention, are used for detecting the polymorphism of the human CYP2C19 gene, the primers, the probes and the kit has the advantages that the sensitivity is high, the specificity is high, the operation is simple, rapid and safe, the result is simply and intuitively determined and read, and blood samples or dried blood spots samples on filter paper can be directly used as templates.

The invention discloses a blood cell morphological analysis internet-of-things detection and diagnosis method and a platform. The method includes the steps that firstly, an operation interface of a computer is displayed to a user, and input information or an operation instruction of the user is received; secondly, a hemogram detection device is connected, a blood image, transmitted by the hemogram detection device, of a current detected person can be displayed on the computer and a mobile terminal synchronously, and an activating blood morphological image and a dried blood morphological image of blood cells can be displayed respectively; thirdly, a save instruction of the user is received, the current blood image, the name of the hemogram and the hemogram are described, identification information of the detected person serves as relevance, and the activating blood morphological image and the dried blood morphological image of the blood cells serve as typical pictures; fourthly, an analysis instruction of the user is received, multiple typical pictures selected by the user are obtained, and a detection analysis report is formed; fifthly, operation instructions transmitted by remote clients including the mobile terminal are received, and corresponding data processing is executed according to the operation instructions.

The invention discloses fertilizer for improving hotbed chives yield. The following constituents in unit by weight are adopted for each mu of land: 400 to 500 kg of rotten sheep manure, 150 to 200 kg of rotten pig manure, 15 to 20 kg of potassium fulvate, 30 to 50 kg of indoleacetic acid, 10 to 15 kg of ammonium hydroxide, 15 to 30 kg of bamboo leaf powder, 30 to 50 kg of biogas residue, 20 to 30 kg of dried blood, and 20 to 40 kg of vermiculite. The fertilizer is simple in matching and convenient to use, and can increase both production and income. The yield is obviously improved on the premise of reducing the use level.

The invention discloses a method for simultaneously detecting multi vitamins in a dry blood spot card. The method comprises the following steps: (1) mixing a dry blood spot card specimen and an extracting solution; oscillating and incubating; then centrifuging and taking supernatant; (2) after uniformly mixing the supernatant obtained by the step (1) with a mobile phase and an internal standard mixed solution, detecting by adopting a tandem mass spectrometry; comparing ion intensity of the vitamins and internal standard of the vitamins to obtain the content of the vitamins in blood serum to be detected or blood plasma to be detected. Furthermore, the invention also discloses a kit for simultaneously detecting the multi vitamins in the dry blood spot card; the kit comprises the internal standard mixed solution, the extracting solution, the mobile phase and a probe washing solution. According to the method disclosed by the invention, multi water-soluble and fat-soluble vitamins are extracted from the dry blood spot card and more than ten vitamins in one specimen can be simultaneously quantified within 2min to 3min by utilizing a tandem mass spectrometer; a detection period is remarkably shortened, and a detection result of the vitamins has higher precision and accuracy.

The invention discloses a formula of a compound feed of hybrid broiler silkie. The feed comprises the following components in parts by weight: 40-50 parts of soybeans, 20-30 parts of corns, 10-15 parts of wheat, 5-10 parts of grouting corn bran, 4-8 parts of lupin residue, 3-5 parts of mung bean flour pulp albumen powder, 2.5-3.5 parts of shiny-leaved yellowhorn pulp, 2-3 parts of squid viscera meal, 2-3 parts of enteromorpha meal, 1.5-2.5 parts of dried blood, 1.4-2.2 parts of crab shell meal, 2-3 parts of composite mountain flour and 0.5-1.5 parts of a premix. The feed is scientific and reasonable in raw material ratio and balanced and comprehensive in nutrition, the growth speed of the hybrid broiler silkie fed by the feed is obviously superior to that of chickens fed by a common feed, the nutrition absorption performance is good, the feed conversion rate is high, and the feed-meat ratio can be reduced by 0.15-0.2. Moreover, the diseases resistance of the hybrid broiler silkie can be greatly improved, the use of chemical medicines is avoided, the survival rate is improved by 1-1.5 percent, and the produced hybrid broiler silkie is high in meat quality, high in nutritive value, safe and free of pollution and does not have drug residues.

The invention relates to woodlouse feed and a processing method thereof, and belongs to the technical field of feed and processing methods thereof. The woodlouse feed is prepared from, by weight, 20-40 parts of wheat bran, 10-35 parts of rice bran, 10-20 parts of bean cakes, 10-30 parts of corn flour, 8-20 parts of highland barley, 2-5 parts of yeast powder, 5-10 parts of dried blood, 3-8 parts of fish meal, 3-8 parts of shrimp meal, 5-10 parts of silkworm chrysalis meal, 3-7 parts of fly maggot meal, 8-18 parts of purslane and 5-15 parts of folium artemisiae argyi. The woodlouse feed is suitable for being eaten by 4-10-year-old woodlouse larva, the added raw materials contain rich protein, multiple vitamins and other nutrient substances, growth of woodlice can be promoted, the growing period of the woodlice can be shortened, the size of the woodlice can be increased, the disease resistance of the woodlice can be improved, and therefore the economic benefits of artificial feeding are effectively increased.

Described are methods and kits for detecting cytomegalovirus DNA in liquid and dried blood samples. Primer and probe combinations for CMV detection are described as well as methods for isolating DNA from blood samples.

A stable blood factor composition contains a stabilizing amount of trehalose in the absence of human serum albumin to provide a product stable at up to 60° C.

The invention provides feed for improving the resistance of leeches. The feed is prepared from snail powder, freshwater mussel powder, dried blood, egg white, soybean meal, activin, rape seed cakes, olive oil, potassium sulphate, monopotassium phosphate, sodium citrate, calcium lactate, calcium hydrophosphate, magnesium sulfate, potassium chloride, lentinan, chitosan, milkvetch root powder, red-rooted salvia root powder, garlic powder, sorbitol, rhizoma atractylodis powder, elecampane powder, compound microbial bacteria, decavitamin and microelements. The feed for improving the resistance of the leeches is reasonable in component, rich and balanced in nutrition, good in palatability, rich in nutritional ingredient and comprehensive in nutrient element, the nutritional ingredients needed by growth of the leeches can be effectively supplemented, growth can be effectively promoted, the growth period of the leaches can be shortened, the breeding cost can be reduced, and economic benefits can be increased; the resistance of the leaches can be improved, the disease resistance can be improved, the diseases of the leaches can be reduced, the breeding cost can be reduced, and the economic benefits can be increased. In addition, the ingredients of the feed are free of pollution to water and environmentally friendly.

The invention relates to a non-fish-meal expanded composite feed for pike and a preparation method thereof, and belongs to the technical field of composite feeds for aquatic products. The method comprises the steps of crushing corn gluten meal, dried blood, soya bean meal, rapeseed meal, cottonseed meal, sunflower seed cake, bran, flour, rice washing bran and wheat; agitating and uniformly mixing with vegetable oil, bile salts, choline chloride, carnitine, premix for fish, bentonite, vitamin E, methionine, lysine, monocalcium phosphate and a potassium sorbate mildew preventive; crushing through an ultramicro crushing machine into a mixture after agitating and uniform mixing; transferring the mixture into a conditioning device; charging steam to condition; transferring into an extrusion expansion granulating machine after conditioning to prepare into feed particles; drying and cooling the expanded feed particles; packing. The non-fish-meal expanded composite feed is complete and rich in nutrition, and can improve the immunity and oxidation resistance of the pike, reduce the stress, improve the disease resistance, improve the palatability and promote the growth.

The invention discloses a type of feed applicable for tilapias intensively cultured in a pond and a preparation method thereof. The feed contains the following ingredients by mass percent: soybean pulp, whole milk puffed soybean, dried blood, rapeseed meal, cottonseed meal, soybeam oil, wheat middling, rice bran, mineral gunk, vitamin gunk, monocalcium phosphate, zeolite powder, bentonite, choline chloride, vitamin C and vitamin E. The preparation method includes the steps of: weighing soybean pulp, whole milk puffed soybean, rapeseed meal and the like according to the mass percent, mixing the mixture to be even, grinding the mixture, mixing the ground mixture with the rest raw materials, tempering and pelleting, drying, removing the powder, inspecting whether the feed is qualified and finally packaging. The raw materials of the feed are common and are low in costs, and the preparation method is simple and reliable; the raw materials are reasonably proportioned; with the improved formulas of the vitamin gunk and the mineral gunk, the feed better satisfies the physiological needs of tilapias intensively cultured in the pond; and after the tilapias with weight of about 180g are fed with the feed disclosed by the invention for two months, the feed coefficient can reach about1.3 and the specific growth rate reaches about 1.9%/d.